Ufuk Universitesi Akademik Acikerisim Sistemi
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Real-Life Experience Regarding Clinical Characteristics and Treatment Outcome in Non-Cutaneous Peripheral T-Cell Lymphomas: A Multicenter Study of the Turkish Hematology Research and Education Group (ThREG)
No full textObjective: Peripheral T-cell lymphomas (PTCLs) are an uncommon and quite heterogeneous group of disorders, representing only 10%-15% of all non-Hodgkin lymphomas. Although both molecular and clinical studies have increased in recent years, we still have little knowledge regarding real-life practice with PTCLs. In this study, we aimed to investigate the clinical characteristics and treatment outcomes of a large population-based cohort of patients presenting with systemic non-cutaneous PTCL. Materials and Methods: We conducted a multicenter retrospective analysis of 190 patients consecutively diagnosed and treated with non-cutaneous PTCLs between 2008 and 2016. Results: Considering all first-line treatment combinations, the overall response rate was 65.9% with 49.4% complete remission (n=81) and 16.5% partial response (n=27). The 5-year overall survival and event-free survival rates were significantly different between the transplant and non-transplant groups (p<0.01, and p=0.033, respectively). Conclusion: The retrospective analysis of a large volume of real-life data on the Turkish experience regarding non-cutaneous PTCL patients showed consistent results compared to other unselected PTCL cohorts with some minor differences in terms of survival and transplantation outcomes. The long-term outcome of patients who receive autologous hematopoietic cell transplantation as part of upfront consolidation or salvage therapy is favorable compared to patients who are unable to receive high-dose therapy
Evaluation of the Prognostic Role of the Systemic Immune Inflammation Index in Postmenopausal Osteoporosis
No full textObjective: The aim of this study is to evaluate the prognos tic role of the systemic immune inflammation index (SII) in postmenopau sal osteoporosis and to examine the SII, neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), mean platelet volume (MPV), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), femur and lumbar bone mineral densitometry (BMD) T scores and their relationships with each other. Material and Methods: 139 patients were included in this retros pective case-control study. Lumbar vertebra 1-4 and femoral neck BMD T scores, ages, ESR, CRP, MPV values were recorded. NLR, PLR, and SII values were calculated. Patients with lumbar and femoral T scores ?-1 were the control group (n=31); with lumbar or femoral T scores between -1 and -2.5 were the osteopenia group (n=38), ?-2.5 were the osteoporosis group (n=37), ?-2.5 and one or more fragility fractures were the severe osteopo rosis group (n=33). Results: SII value was significantly higher in the os teoporosis group compared to the osteopenia group (p=0.004). SII value had a diagnostic value in predicting osteoporosis in patients with osteopenia (area under curve: 0.686, %95 confidence interval: 0.565-0.806, p0.05). Conclusion: SII value can be a prognostic predictor that can be used to predict the development of osteoporosis in postmenopausal osteo penic patients
Effects of thiostrepton alone or in combination with selumetinib on triple-negative breast cancer metastasis
No full textObjective FoxM1 transcription factor contributes to tumor metastasis and poor prognosis in many cancers including triple-negative breast cancer (TNBC). In this study, we examined the effects of FoxM1 inhibitor Thiostrepton (THIO) alone or in combination with MEK inhibitor Selumetinib (SEL) on metastatic parameters in vitro and in vivo. Methods Cell viability was determined by MTT assay. Immunoblotting and immunohistochemistry was used to assess metastasis-related protein expressions in 4T1 cells and its allograft tumor model in BALB/c mice. In vivo uPA activity was determined by enzymatic methods. Results Both inhibitors were effective on the expressions of FoxM1, ERK, p-ERK, Twist, E-cadherin, and Vimentin alone or in combination in vitro. THIO significantly decreased 4T1 cell migration and changed the cell morphology from mesenchymal-like to epithelial-like structure. THIO was more effective than in combination with SEL in terms of metastatic protein expressions in vivo. THIO alone significantly inhibited mean tumor growth, decreased lung metastasis rate and tumor foci, however, no significant changes in these parameters were observed in the combined group. Immunohistochemically, FoxM1 expression intensity was decreased with THIO and its combination with SEL in the tumors. Conclusions This study suggests that inhibiting FoxM1 as a single target is more effective than combined treatment with MEK in theTNBC allograft model. The therapeutic efficacy of THIO should be investigated with further studies on appropriate drug delivery systems.Scientific and Technological Research Council of Turkey, (TUBITAK) [1002116S162]; Teaching Staff Training Program of TurkeyThis work was supported by the Scientific and Technological Research Council of Turkey, (TUBITAK) under Grant (number 1002116S162) and Teaching Staff Training Program of Turkey
The association of antiviral drugs with COVID-19 morbidity: The retrospective analysis of a nationwide COVID-19 cohort
No full textBackground and objectivesAlthough several repurposed antiviral drugs have been used for the treatment of COVID-19, only a few such as remdesivir and molnupiravir have shown promising effects. The objectives of our study were to investigate the association of repurposed antiviral drugs with COVID-19 morbidity. MethodsPatients admitted to 26 different hospitals located in 16 different provinces between March 11-July 18, 2020, were enrolled. Case definition was based on WHO criteria. Patients were managed according to the guidelines by Scientific Board of Ministry of Health of Turkey. Primary outcomes were length of hospitalization, intensive care unit (ICU) requirement, and intubation. ResultsWe retrospectively evaluated 1,472 COVID-19 adult patients; 57.1% were men (mean age = 51.9 +/- 17.7years). A total of 210 (14.3%) had severe pneumonia, 115 (7.8%) were admitted to ICUs, and 69 (4.7%) were intubated during hospitalization. The median (interquartile range) of duration of hospitalization, including ICU admission, was 7 (5-12) days. Favipiravir (n = 328), lopinavir/ritonavir (n = 55), and oseltamivir (n = 761) were administered as antiviral agents, and hydroxychloroquine (HCQ, n = 1,382) and azithromycin (n = 738) were used for their immunomodulatory activity. Lopinavir/ritonavir (beta [95% CI]: 4.71 [2.31-7.11]; p = 0.001), favipiravir (beta [95% CI]: 3.55 [2.56-4.55]; p = 0.001) and HCQ (beta [95% CI]: 0.84 [0.02-1.67]; p = 0.046) were associated with increased risk of lengthy hospital stays. Furthermore, favipiravir was associated with increased risks of ICU admission (OR [95% CI]: 3.02 [1.70-5.35]; p = 0.001) and invasive mechanical ventilation requirement (OR [95% CI]: 2.94 [1.28-6.75]; p = 0.011). ConclusionOur findings demonstrated that antiviral drugs including lopinavir, ritonavir, and favipiravir were associated with negative clinical outcomes such as increased risks for lengthy hospital stay, ICU admission, and invasive mechanical ventilation requirement. Therefore, repurposing such agents without proven clinical evidence might not be the best approach for COVID-19 treatment
Bortezomib Potentiates the Effect of Roscovitine Via Dna Damage Induced Apoptosis in A549 Lung Cancer Cells
No full textThe adoption of new treatment modalities remains crucial as lung cancer has one of the lowest survival rates, along with liver and pancreatic cancer. Bortezomib is a proteasome inhibitor that has higher anticancer effect in combination therapies. Therefore, the aim of this study is to investigate whether bortezomib could have additional anticancer effect when combined with cyclin-dependent kinase (CDK) inhibitor-roscovitine in vitro. Apoptosis related gene expression levels of p53, Noxa, Puma, Bcl-xL, Bak, Casp-3 and Casp-7 were measured by quantitative PCR (qPCR) upon treatment with 10-20?M roscovitine and in combination with 30nM bortezomib for 24 hours. Synergistic effect on apoptosis was also investigated at protein levels by analyzing p53, Cleaved Casp-3 and Cleaved Parp expressions. Induction of autophagy was determined by western blotting of B-catenin and LC3B I-II. Roscovitine combined bortezomib treatment induced apoptosis by upregulating p53 pathway and its downstream mediators. Bortezomib increased Parp and Caspase3 cleavage significantly at 24h. Bortezomib inhibited B-catenin and triggered autophagy induction at 24 and 48hours. As cancer cells evade programmed cell death, CDK inhibitors might be used to direct cancer cells into apoptosis. This study concludes that bortezomib potentiates the effect of roscovitine via DNA damage induced apoptosis in A549 lung cancer cell
Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection
No full textHealthcare-associated infection (HCAI) is a global health challenge and methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamases (ESBL) producing gram-negative bacilli, which were isolated from HCAIs, are the most common antimicrobial-resistant pathogens. The present research aimed to determine the chemical composition and antimicrobial activity of ethyl acetate and methanol extracts of Nigella sativa seeds. Antimicrobial activity of plant extracts was observed against 20 clinical yeast (which isolated from swab samples of tongue dorsal surfaces in patients with stomatitis), 18 clinical bacteria isolates (which found from HCAIs), and also eight bacteria, and three yeast standard strains by using a broth microdilution assay. The methanol extract had the highest inhibitory activity against S. aureus standard strain at 0.4 mg/mL. The ethyl acetate extract showed the highest antibacterial activity against Bacillus cereus standard strain at 0.4 mg/mL. The methanol and ethyl acetate extracts did not show inhibitory activity against Gram-negative bacteria and yeast isolates. The LC-Q-TOF-MS profiles of the extracts were found to contain saponin, alkaloid, and fatty acid. Mass spectra revealed the presence of 14 fatty acids, four saponins, and three alkaloids for methanol extract and 13 fatty acids, three saponins, and two alkaloids for ethyl acetate extract. © 2022 Marmara University Press.Gazi ÜniversitesiThe authors would like to thank Prof. Dr. Tijen ONKOL from the Pharmacy Faculty of Gazi University for providing helping at the laboratory
Obsesif Kompulsif Bozukluk Tanılı Ergenlerin ve Annelerin Obsesif İnançları ve Çıkarımsal Karmaşaları Arasındaki İlişki
No full textAmaç: Obsesif kompulsif bozukluk(OKB) tanılı ergenlerin ve annelerinin obsesif inançları ve çıkarımsal karmaşaları arasındaki ilişkiyi saptayarak OKB’deki bilişsel değerlendirmeleri araştırmaktır. Gereç ve Yöntem: Çalışmaya 25 OKB hastası (20 erkek ve 5 kız) ile bu hastaların anneleri dahil edildi. Hastalara Okul Çağı Çocukları İçin Duygulanım Bozuklukları ve Şizofreni Görüşme Çizelgesi-Şimdi ve Yaşam Boyu Şekli (K-SADS-PL) ve Yale-Brown Obsesyon-Kompulsiyon Ölçeği (Y-BOKÖ) uygulandıktan sonra OKB tanısı alan hastalara ve onların annelerine Obsesif İnançlar Ölçeği-44 (OİÖ-44), Çıkarımsal Karmaşa Ölçeği (ÇKÖ), Padua Envanteri (PE) uygulanmıştır. Hastaların ve annelerinin obsesif inançları, çıkarımsal karmaşaları ile obsesif kompulsif belirti puanlarının korelasyonu incelenmiştir. Bulgular: Ergenler ile annelerin, obsesif inançları ve çıkarımsal karmaşa ölçeği arasında yapılan korelasyon analizlerine göre sadece ergenlerin obsesif inançlardan mükemmeliyetçilik/kesinlik puanları ile annelerin abartılmış sorumluluk/tehlike puanları arasında istatistiksel olarak negatif yönde anlamlı korelasyon bulundu(r = -0.42, p = 0.039). Sonuç: Bu çalışma OKB tanılı ergenlerdeki obsesif inançlar ve çıkarımsal karmaşa ile ilgili hipotezlerini kısmen destekler. Klinik tedaviler ailelerdeki olası patolojik bilişsel faktörler göz önüne alınarak düzenlenmesi tedavi başarısını artırabilir
Chronic obstructive pulmonary disease phenotypes in Turkey: the COPET study-a national, multicenter cross-sectional observational study
No full textBackground/aim: While mortality rates decrease in many chronic diseases, it continues to increase in COPD. This situation has led to the need to develop new approaches such as phenotypes in the management of COPD. We aimed to investigate the distribution, characteristics and treatment preference of COPD phenotypes in Turkey. Materials and methods: The study was designed as a national, multicenter, observational and cross-sectional. A total of 1141 stable COPD patients were included in the analysis. Results: The phenotype distribution was as follows: 55.7% nonexacerbators (NON-AE), 25.6% frequent exacerbators without chronic bronchitis (AE NON-CB), 13.9% frequent exacerbators with chronic bronchitis (AE-CB), and 4.8% with asthma and COPD overlap (ACO). The FEV 1 values were significantly higher in the ACO and NON- AE than in the AE-CB and AE NON-CB (p < 0.001). The symptom scores, ADO (age, dyspnoea and FEV 1) index and the rates of exacerbations were significantly higher in the AE-CB and AE NON-CB phenotypes than in the ACO and NON-AE phenotypes (p < 0.001). Treatment preference in patients with COPD was statistically different among the phenotypes (p < 0.001). Subgroup analysis was performed in terms of emphysema, chronic bronchitis and ACO phenotypes of 1107 patients who had thoracic computed tomography. A total of 202 patients had more than one phenotypic trait, and 149 patients showed no features of a specific phenotype. Conclusion: Most of the phenotype models have tried to classify the patient into a certain phenotype so far. However, we observed that some of the patients with COPD had two or more phenotypes together. Therefore, rather than determining which phenotype the patients are classified in, searching for the phenotypic traits of each patient may enable more effective and individualized treatment
Beliefs About Medicines and Adherence to Treatment in Turkish Patients with Inflammatory Bowel Disease
No full textBackground: Although studies are investigating the perception and beliefs about treatment and adherence to treatment in different societies related to inflammatory bowel disease, there are no studies on this subject in Turkish people with different sociocultural structures. In our study, we aimed to evaluate the beliefs about treatment and its effect on adherence to treatment in the Turkish population with inflammatory bowel disease. Methods: In the study, the Medication Adherence Report Scale and Beliefs about Medicines Scale scales were used to evaluate the treatment compliance and perception and beliefs about treatment. Characteristics that could affect treatment compliance were evaluated by statistical analysis. Results: A total of 253 patients, 167 with ulcerative colitis and 86 with Crohn's disease, were included in the study. The non-adherence rate to the treatment was found as 41.9% in ulcerative colitis and 24.4% in Crohn's disease (P =.006). Intentional (29.3% in ulcerative colitis and 16.3% in Crohn's disease [P =.031] and unintentional non-adherence to treatment (28.1% in ulcerative colitis, 16.3% in Crohn's disease [P =.037] were significantly higher in ulcerative colitis than in Crohn's disease. Female gender (odds ratio = 2.59, P =.005), low education level (odds ratio = 4.8, P =.015), distal involvement in ulcerative colitis (P =.014), and thoughts about the disease would last too soon in Crohn's disease (odds ratio = 4.17, P =.049) were risk factors for non-adherence to treatment. Conclusion: The negative perception of treatment in inflammatory bowel disease affects adherence to the treatment. Considering some social factors that affect adherence to the treatment and taking measures to enhance the adherence to treatment will increase the success of treatment
The Evaluation of Migraine Frequency in Patients with Psoriasis
No full textAim: Psoriasis, previously thought to be a disease restricted to the skin, is now considered systemic, accompanied by numerous comorbidities. Our study aimed to establish the migraine frequency in psoriasis patients and assess the relationship between the severity and duration of psoriasis and the frequency and severity of migraine. Material and Method: This case-control study was performed in the dermatology outpatient clinic. A total of 80 people, including 40 patients over the age of 18 who applied to the outpatient clinic and were diagnosed with psoriasis, and 40 people with similar gender and age characteristics and other skin problems, were included in the control group. Results: The frequency of migraine in psoriasis patients was 35.0% and 15.0% in the control group. In psoriasis patients, the median Psoriasis Area and Severity Index (PASI) score was 3.60 (1.20–13.20) in patients without migraine and 2.90 (1.20–12.00) in patients with migraine. Migraine frequency was 45.0% in patients suffering from the disease for more than eight years and 25.0% in patients suffering for eight years or less. The incidence of migraine was significantly higher in patients with psoriasis for more than eight years than in the control group. Conclusion: Our study presented important outputs that the severity and duration of psoriasis disease might be related to migraine disease