JIKSH: Jurnal Ilmiah Kesehatan Sandi Husada
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Molecular characterization of selected endophytic fungi isolate IDGG 3 leaf galing galing (cayratia trifolia L.) with the polymerase chain reaction method
Full text linkBackground: Endophytic fungi live in plant tissues and are usually not harmful to their host plants. One of the host plants for endophytic fungi is bush grape leaves (Cayratia trifolia L.).
Objective: The research aimed to determine the molecular characteristics of the endophytic fungi isolates selected IDGG 3 of bush grape leaves.
Methods: The test used the polymerase chain reaction method to determine the species-level characteristics using internal transcribed spacers (ITS) 1 and 4. The DNA band was successfully amplified with 500 base pairs with 3000 markers.
Results: The fungi isolates selected IDGG 3 of bush grape leaves in molecular identification based on the Basic Local Alignment Search Tool (BLAST) analysis on Genebank NCBI that the IDGG 3 samples of bush grape leaves had a similarity level of 99%, namely Fusarium incarnatum JL5-2, Fusarium incarnatum JL3-4- 1, Fusarium incarnatum CBB-2, Fusarium incarnatum JL3-3, Fusarium incarnatum CBA-3, Fusarium incarnatum CBB-1, Fusarium incarnatum CBA-2, Fusarium chlamydospore, Fusarium cf. Incarnatum, and Fusarium sp.
Conclusion: The results of the molecular characteristics of the selected endophytic fungi isolate IDGG 3 galing-galing leaves (Cayratia trifoliata L.) have the closest degree of kinship with the species Fusarium incarnatum JL3-4-1. The importance of the PCR method in the molecular characterization of endophytic fungi, as well as opening opportunities for further exploration of the biotechnological potential of endophytic fungi from Cayratia trifolia L
Analysis of blood calcium levels in calcium water consumers
Full text linkIntroduction: Water is a primary and basic human need. One of the chemical parameters that must be met for drinking water needs is mineral content, such as calcium and magnesium. Water hardness is a condition in which there is excessive calcium and magnesium content, which is commonly known as calcareous water. If consumed long-term, high-water hardness that exceeds normal limits can interfere with body health. Specifically, excess calcium in the body can cause hypercalcemia, hyperparathyroidism, kidney stones, and muscle tissue damage (muscle weakness).
Objective: This study aims to determine blood calcium levels in individuals who consume calcareous healthy water.
Methods: The type of research used is descriptive-analytical, with blood calcium level analysis carried out using the Ion Selective Electrode (ISE) method through the Electrolyte Analyzer (CBS400).
Result: Based on the results of the study, it was obtained that blood calcium levels in 30 community respondents in Ranomeeto District (100%) were above the reference value, indicating high blood calcium levels.
Conclusion: blood calcium levels in people who consume lime juice indicate high blood calcium levels. Consuming water with a high lime content can hurt health. Therefore, people need to consider the quality of the drinking water they drink, especially those that have a high lime content, to maintain heart and kidney health in the long term
Dental odyssey: a comprehensive literature review on global strategies, behavioral programs, and creative approaches for ages 6-12 years
Full text linkIntroduction: Oral and dental hygiene serves as an indicator of a condition free from diseases, debris, and health issues in the oral cavity, affecting individuals of all age groups. Health education through various global learning systems, behavioural programmes, and creative approaches has proven effective in enhancing children's understanding of the importance of maintaining oral and dental cleanliness.
Objective: Objective: This research aims to review relevant journals contributing to the development of insights, behaviours, and understanding regarding children's oral and dental health across crucial age ranges.
Methods: Using a literature review method, we filtered articles from Google Scholar, PubMed, Garuda (Digital Reference Library), and Science Direct published within the last six years. Searches in both Indonesian and English languages yielded ten articles that, after a selection process, provided diverse results regarding the implementation of learning models to enhance children's understanding of their oral and dental hygiene.
Result: From the ten articles, it can be concluded that the application of education on oral and dental hygiene through various learning systems can effectively improve children's knowledge.
Conclusion: the oral hygiene of elementary school children from various countries. Variations in research designs were found, and there is low awareness among children regarding dental health in Indonesia. Educational programmes, such as "Brush Day & Night," and collaborations between FDI and Unilever have proven effective in improving children's toothbrushing habits. The use of music and the snake ladder game was also considered appropriate for developing awareness and behaviours related to dental hygiene
Effectiveness of giving dragon fruit and ambon banana to increase hemoglobin levels in anemic adolescent girls
Full text linkBackground: Anaemia is a common health problem among adolescent girls, especially in areas with limited access to nutritious food. One of the efforts to overcome anaemia is to increase the consumption of iron-rich fruits, such as dragon fruit and ambon banana.
Objective: to evaluate the effectiveness of dragon fruit and ambon banana administration in increasing haemoglobin levels in anaemic adolescent girls
Method: Qualitative research combines observational and interventional approaches. The research sample amounted to 2 adolescent female respondents. Instrument of treatment activities using a checklist sheet on elevated haemoglobin levels.
Results: An increase in haemoglobin levels was obtained in adolescents given dragon fruit juice, where the first week was 10.1 g / dl, the second week was 11.2 g / dl, and the fourth week was 12.3 g / dl. There was a slightly slower increase in haemoglobin levels in adolescents who were given Ambonese bananas in the first week of 10.2 g / dl, the second week of 10.8 g / dl, and the fourth week of 11.3 g / dl.
Conclusion: Respondents who consume dragon fruit increase haemoglobin levels faster than bananas. It is expected to be used as an alternative midwifery care for the midwife profession by giving dragon fruit juice to increase haemoglobin levels. Teenagers are advised to maintain a balanced diet by consuming fruits rich in iron and nutrients, such as dragon fruit, ambon, bananas, and other essential nutrients. By maintaining a balanced diet and increasing the consumption of iron-rich fruits, adolescents can improve their haemoglobin levels and maintain overall body health
Health education lecture method to increase posyandu cadres' knowledge about stunting prevention in children
Full text linkIntroduction: Stunting is a serious health problem in children, especially in developing countries. Sufficient knowledge about stunting prevention is essential for Posyandu cadres, as they play a role in providing information and support to mothers and children in the community.
Objective: evaluate the effectiveness of the lecture method in increasing the knowledge of Posyandu cadres about stunting prevention in children.
Method: This type of research uses a quantitative approach. In this study, using a group of health posyandu cadres, stunting knowledge was given using the lecture method. The population in the study of cadre mothers was 60 people, with a sample size of 36 people, using purposive sampling. Measuring instruments use questionnaire instruments and analysis results are in the form of descriptive data equipped with tables.
Results: based on statistical test wilcoxon test Asymp.Sig. (2-tailed) P Value 0.000 ≤ 0.05 means that there is an increase in knowledge between before and after educational intervention with the lecture method.
Conclusion: The lecture method is effective in increasing the knowledge of Posyandu cadres about stunting prevention in children. It is hoped that the results of this study can be the foundation for a broader health education program to overcome the problem of stunting in children in the community
Analysis of reconstitution process, stability, stability of intravenous antibiotics in hospitalised patients in a private hospital
No full textIntroduction: reconstitution of sterile preparations is a series of changes in the drug from its original condition to a new product by dissolving or adding other ingredients carried out aseptically by the pharmacy.
Aim: determine the suitability of the reconstitution technique, the suitability of stability (solvent type and solvent volume), and the suitability of the sterility of the reconstitution product.
Methods: data collection obtained by observation is carried out with the help of a checklist that is adapted to hospital standard operating procedures (SOPs), Inject-able Drug Guide, guidelines for mixing inject-able drugs, and preparations. 48 processes of reconstitution of intravenous antibiotics were carried out in hospitals that could be observed as samples.
Results: conformity to the preparation stage were 99%, the mixing stage was 73%, the labelling was 100%, and the labelling was 90%. In evaluating the stability of the solvent type category, the suitability of 100%. In the volume category of the solvent used, 90% were suitable, and 10% were unsuitable for the antibiotic’s cefotaxime 1 gram, vancomycin 1 gram, and sulbactam + ampicillin Na 1 gram. From the sterility test results, the reconstitution of ceftriaxone intravenous antibiotic preparations showed that the bacterial preparations were free of microorganisms (sterile).
Conclusion: the personnel performing the reconstitution are nurses trained in intravenous antibiotic recovery. One of the factors that caused the reconstitution process not to be under the hospital's KSB was the lack of training and supervision from the hospital management. SPO recovery is not installed in an intravenous drug mixing room
The physical quality test formulation of ethyl acetate extract of kawista fruit peel (Limonia Acidissima L.) Ointment as an antibacterial against propionibacterium acnes
Full text linkIntroduction: Using medicinal plants in Indonesia still needs to be optimized. Almost all parts of the Kawista plant have been traditionally used to treat various ailments such as antioxidants, antidiabetic, wound healing, and controlling uric acid levels. Existing research on Kawista fruit peel as an acne antibacterial includes studies on the ethyl acetate extract of Kawista fruit peel against Propionibacterium acnes, which falls into the moderate to strong category. In Indonesia, Kawista fruit is limited to the flesh and is used to make syrup and dodol (a traditional sweet). In contrast, the fruit's peel remains a waste product.
Objective: This study aims to develop a formula for an ethyl acetate extract ointment of Kawista fruit peel (Limonia acidissima L.) as an antibacterial agent against Propionibacterium acnes with good physical quality.
Methods: The fruit peel extract was obtained using the maceration method with 96% ethanol. The extract was then formulated into ointment preparations with concentration variations of 6.25% (F1), 12.50% (F2), and 25% (F3). The physical stability tests for the ointment included organoleptic parameters, homogeneity, spreadability, and pH value tests. The data were analyzed to determine the effect of the extract concentration and storage time on the ointment's physical stability.
Result: The test results showed that the ointment formulation of Kawista fruit peel extract (Limonia acidissima L.) could be successfully formulated into an ointment form and met the required evaluation criteria, including organoleptic testing, homogeneity testing, pH testing, and spreadability testing.
Conclusion: Based on the study, it can be concluded that the Kawista fruit peel extract ointment (Limonia acidissima L.) can be successfully formulated into an ointment and meets the required evaluation criteria, including organoleptic testing, homogeneity testing, pH testing, and spreadability testing. A skin irritation test is required to determine whether any irritation occurs after applying the ointment
Analysis of stunting prevention through communication, information and education with empowerment
Full text linkIntroduction: Stunting is a chronic health problem characterized by a child's height being lower than their age standard, often caused by poor nutritional intake and recurrent infections. This research focuses on stunting prevention through Communication, Information, and Education (KIE) with a community empowerment approach.
Research Methodology: This type of research is observational analytics with a cross-sectional approach. The research sample consisted of mothers under five who met the inclusion criteria. Data were collected through questionnaires and interviews and then analyzed using the Wilcoxon statistical test. The intervention was carried out by providing education through digital-based KIE media, developed using the Borg & Gall model.
Result: The analysis showed a significant improvement in the knowledge and attitude of mothers under five after the intervention. Before the intervention, 74% of mothers had low knowledge about stunting, but after the intervention, this figure dropped to 2%, with 98% of mothers having good knowledge. The mother's attitude also increased from 2% good to 96% good after the intervention. The results of the Wilcoxon test showed a p-value of 0.001 for knowledge and 0.005 for attitude, showing a significant influence of KIE media on stunting prevention.
Conclusion: The provision of digital-based KIE with a community empowerment approach has proven to be effective in increasing the knowledge and attitude of mothers under five towards stunting prevention. Implementing KIE is expected to be a sustainable strategy to support the national program in reducing stunting rates. Further research is suggested to compare the effectiveness between the intervention and control groups
Effect of anemia in pregnant women on the incidence of premature labor and low birth weight
No full textIntroduction: Anemia in pregnant women is one of the health problems that often occur and can have a negative impact on pregnancy. Iron and other nutrient deficiencies during pregnancy can increase the risk of complications, including premature labor and low birth weight (BBLR). This study aims to analyze the effect of anemia in pregnant women on premature labor and BBLR incidence.
Research Methodology: This study uses an observational design with a retrospective cohort approach. Data is collected from the medical records of pregnant women who have given birth in the hospital for a certain period. The research sample consisted of pregnant women who experienced anemia and those who did not experience anemia.
Result: The results showed that pregnant women with anemia had a higher risk of preterm labor compared to pregnant women without anemia (OR = 2.5; p < 0.05). In addition, the prevalence of babies with low birth weight was higher in mothers with anemia (OR = 3.1; p < 0.05). The results of the multivariate analysis showed that anemia in pregnant women was an independent risk factor for the incidence of premature labor and BBLR after being controlled by other factors such as maternal age, nutritional status, and obstetric history.
Conclusion: Anemia in pregnant women has a significant effect on the increased risk of premature labor and low birth weight. Therefore, efforts to prevent and treat anemia during pregnancy must be improved through early screening, nutrition education, and iron and folic acid supplementation to reduce adverse impacts on mothers and babie
Education has a significant effect on the performance of implementing nurses in hospitals
No full textIntroduction: Higher education typically gives nurses a more profound knowledge of healthcare practices and recent developments and enhances nurses' analytical and problem-solving abilities, enabling them to provide more effective care.
Aim: Knowing education significantly affects the performance of implementing nurses in hospitals.
Methods: The research design is qualitative analytical descriptive research with a cross-sectional approach. The population is all nurses working in the internal care room, and as many as 50 responses using the sampling technique are total sampling.
Results: Showing the chi-square test with the level of meaning (α) = 0.05, obtained p value = 0.003, education significantly influences the implementing nurse's performance.
Conclusion: Education significantly influences the performance of implementing nurses, and continuing education can be an effective strategy for improving the standard of health care in hospitals. Nurses with higher education tend to have better clinical knowledge and skills, enabling them to provide higher-quality care