Online Journal Faculty of Medicine Universitas Sriwijaya
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The Hepatoprotective Effects of Basil Leaf (Ocimum sanctum L.) Extract on Paracetamol Induced Liver Damage in Male Rat
No full textBasil leaf have antioxidants such as flavonoids, so it is thought to have a hepatoprotective effect. This study aims to investigate the effect of basil leaf extract on SGOT and SGPT levels in male rats induced by paracetamol. Basil leaf extract was carried out by the percolation method using ethyl acetate solvent, Some 20 male sprague dawley rats were randomly divided into 5 groups. Basil leaf extract (400 mg/kgBB and 600 mg/kgBB) and sylimarin (100 mg/kgBB) were carried out every day for 28 days, paracetamol was induced 24 hours after giving the last day of basil leaf extract. The parameters measured were SGOT and SGPT level to assess the effect of basil leaf extract on liver damage caused by paracetamol. The results showed that basil leaf extract (400 mg/kgBB dan 600 mg/kgBB) showed that the activities of SGOT and SGPT levels were statistically significant (p<0,05) to negative control. Basil leaf extract shows the effect of hepatoprotector on liver induced by paracetamol, however the effect given was not able to equate with positive control
Granzyme-B Expression as a Predictor of Immune Surveillance (Correlation Study in Patient with Squamous Cell Carcinoma and Uterine Cervical Dysplasia)
No full textBackground: The most common histologic type of cervical cancer is squamous cell carcinoma (SCC), with the risk of recurrence depending on immune surveillance with granzyme-B markers, and tumor infiltrating lymphocytes (TIL) is an immunological determinant associated with a better prognosis. Granzyme-B is produced by TIL. The study was aimed to prove the difference in expression of granzyme-B in SCC epithelial cells with cervical dysplasia precancerous lesions as a predictor of immune surveillance. Methods: Correlation analytic study with cross-sectional design on paraffin block patients with SCC and cervical epithelial dysplasia at the Anatomical Pathology Laboratory, RSUP Dr. Kariadi, Semarang, during 2018 and 2019, using granzyme-B immunohistochemistry assays, assessed TIL activation. Results: Most cases of cervical SCC were 40 years old, 50% were stage IIIB, 20% with the final condition died on observation 1 year after the initial diagnosis was made. The uterine cervical SCC group expressed granzyme-B with a mean score of 5.81±1.17 (p=0.001) and the dysplastic epithelial lesion group with a mean score of 6.83±0.95 (p=022), the Mann-Whitney test showed p value = 0.009, so that the test results are statistically significant. Conclusion: Expression of granzyme-B in dysplasia is higher than in uterine cervical SCC.
 
Hepatitis Lupus in Systemic Lupus Erythematosus in Male Patients
No full textSystemic Lupus Erythematosus (SLE) is a complex autoimmune disease characterized by autoantibodies against the cell nucleus and involves many organ systems in the body with unknown etiologies and various clinical manifestations, disease course and prognosis. SLE can be found at all ages, generally appearing at age 9-58 years with a peak at age 28 years. It is more common in women with a ratio of women to men 15: 1 to 22: 1. The highest incidence and prevalence of SLE was found in North America 23.2 / 100.000 population / year and 241 / 100.0000 population. In Indonesia, there has been an increase in visits to SLE patients from 17.9-27.2% in 2015 to 30.3-58% in 2017. One of the manifestations of SLE is hepatitis lupus, which is inflammation of the liver tissue. Lupus hepatitis can occur in 20-50% of patients with SLE. It was reported that a 20-year-old man presented with complaints of pain in the joints of the right and left hands which increased since 1 week. The patient also complained of reddish patches on the face, hair loss and mouth sores. Physical examination revealed anemic eye conjunctiva, malar rash, oral ulcer. During the joint examination, there was tenderness in bilateral MCP and PIP. The abdominal examination revealed hepatomegaly. Investigations revealed anemia, thrombocytopenia, increased liver function. Abdominal ultrasound revealed hepatomegaly. ANA profile examination was positive for anti RNP, anti-sm, and anti- ribosomal protein antibodies. The patient was diagnosed with Systemic Lupus Erythematosus with lupus hepatitis according to the ACR (American College of Rheumatology) criteria in which the patient had 6 criteria. The patient was given therapy with 2x125 mg of intravenous methyl prednisolone for 3 days and hydroxychloroquine 1x200 mg orally and other symptomatic drugs
Hubungan Aktivitas Fisik, Kualitas Tidur, dan Indeks Massa Tubuh dengan Kadar HbA 1c Pada Pasien DM Tipe 2 yang Datang ke Poliklinik Endokrin Metabolik Diabetik di RSUP DR. Mohammad Hoesin Palembang
No full textDiabetes melitus merupakan salah satu kelompok penyakit metabolik kronis yang ditandai oleh hiperglikemia karenagangguan sekresi insulin, kerja insulin, atau keduanya. Peningkatan jumlah penderita DM tipe 2 berkaitan denganfaktor risiko yang tidak dapat diubah dan faktor risiko yang dapat diubah. Penyakit DM tipe 2 dapat dipantau melaluipengontrolan glukosa darah jangka panjang dengan melakukan pemeriksaan HbA 1c. Penelitian ini bertujuan untukmengetahui hubungan faktor-faktor resiko DM tipe 2 dengan kadar HbA 1c sebagai indikator glikemik. Penelitian inimenggunakan rancangan cross sectional. Populasi penelitian ini adalah semua pasien yang datang ke poliklinikendokrin RSUP Dr. Mohammad Hoesin Palembang pada bulan November-Desember 2016. Didapatkan 47 sampel yangdiambil dengan teknik consecutive sampling. Data primer diambil dengan cara wawancara langsung dan data sekunderdiambil dari rekam medis di Bagian Instalasi Rekam Medis RSUP Dr. Mohammad Hoesin Palembang. Data yangdiperoleh dianalisis menggunakan uji Fisher Test dan uji regresi logistik. Pada uji Fisher Test diketahui terdapathubungan antara aktivitas fisik dan kadar HbA 1c (p=0,000), terdapat hubungan antara kualitas tidur dan kadar HbA 1c(p=0,001), dan tidak ada hubungan antara indeks massa tubuh dan kadar HbA 1c (p=0,128). Pada analisis multivariatdiketahui kualitas tidur merupakan faktor yang paling mempengaruhi kadar HbA1c (OR=0,038)
Korelasi Luaran dan Lama Perawatan Janin dengan Ekstraksi Vakum pada Persalinan Kala II Lama
No full textSecara global 23%, dari kematian neonatal dikaitkan dengan asfiksia lahir. Beberapa penelitian menyebutkanfaktor risiko yang berhubungan dengan nilai APGAR pada kejadian asfiksia neonatorum diantaranya yaitupersalinan (partus lama dan jenis persalinan). Penelitian ini bertujuan untuk mengetahui korelasi luaran dan lamaperawatan janin dengan ekstraksi vakum pada persalinan kala II lama di RSUP Dr. Moh. Hoesin Palembang periodetahun 2012-2014. Penelitian ini adalah penelitian deskriptif observasional, dengan desain case series pada pasienkala II lama yang diekstraksi vakum di RSUP Dr. Moh. Hoesin Palembang 2012-2014. Populasi penelitian adalahseluruh pasien kala II lama di RSUP Dr. Moh. Hoesin Palembang tahun 2012-2014. Sampel penelitian adalah pasienkala II lama yang diekstraksi vakum di RSUP Dr. Moh. Hoesin Palembang tahun 2012-2014. Pasien kala II lama yangdiekstraksi vakum di RSUP Dr. Mohammad Hoesin Palembang tahun 2012-2014 dengan usia terbanyak (83,7%)adalah kategori usia 20-35 tahun, nilai APGAR menit ke-1 terbanyak (87,4%) adalah nilai APGAR 7-10, nilai APGARmenit ke-5 terbanyak (100%) adalah nilai APGAR 7-10. Terdapat korelasi bermakna antara nilai APGAR menit ke-1dan tindakan vakum (r= 0,387 p=0,000) dan ada korelasi antara nilai APGAR menit ke-1 dan lama perawatan janin(r= 0,33 p=0,03)
Rasionalitas Penggunaan Antagonis Kalsium Pada Wanita Hamil
No full textPreeklampsia adalah suatu keadaan hipertensi yang timbul setelah 20 minggu kehamilan disertai denganproteinuria. Eklampsia adalah preeklampsia yang disertai dengan kejang dan atau koma. Di Indonesiapreeklampsia dan eklampsia masih merupakan salah satu penyebab utama kematian ibu dan perinatal. Penelitianini bertujuan untuk mengetahui pola pemberian antagonis kalsium pada wanita hamil di Rumah Sakit MohammadHoesin Palembang. Survei penggunaan obat dilakukan di Ruang Rekam Medis pada November sampai Desember2013. Populasi penelitian adalah semua pasien hipertensi pada wanita hamil di Instalasi Rawat Inap DepartemenObstetri dan Ginekologi Rumah Sakit Mohammad Hoesin. Sampel berjumlah 63 penderita yang memenuhi kriteriainkulsi. Hasil penelitian bentuk deskriptif dan dalam tabel persentase. Didapatkan 63 wanita hamil yang hipertensi.Hipertensi pada wanita hamil ditemukan pada usia 33-37 tahun (40%). Hipertensi ditemukan pada kehamilanpertama (11.3%). Jenis hipertensi terbanyak adalah derajat dua (68.2%). Antagonis kalsium yang digunakan adalahnifedipin dan amlodipin. Nifedipin diberikan peroral dengan dosis 10 mg satu kali makan dengan frekuensi tiga kalidalam satu hari (100%). Amlodipin diberikan peroral dengan dosis 10 mg satu kali makan dengan frekuensi satukali dalam satu hari (100%). Magnesium sulfat sering digunakan bersamaan dengan antagonis kalsium (89%).Penggunaan antagonis kalsium pada wanita hamil di Departemen Obstetri dan Ginekologi Rumah SakitMohammad Hoesin sudah rasional
Sekresi IFN-γ dan IL-10 Setelah Stimulasi Antigen Fusi ESAT-6-CFP-10 (EC610) pada Penderita TB Aktif dan TB Laten
No full textSistem imunitas seluler sangat berperan dalam melawan infeksi TB yaitu peran sel limfosit T yang berdiferensiasimenjadi sel Th1 mensekresikan sitokin pro inflamasi IFN-γ dan sel Th2 yang mensekresi sitokin anti inflamasi IL-10.Antigen fusi EC610 bersifat spesifik dan memiliki antigenitas yang kuat terhadap stimulasi sel T. Tujuan penelitian untukmengetahui perbedaan rerata kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610 pada penderita TB aktif dan TBlaten. Desain penelitian ini adalah eksperimen semu secara in vitro dengan kultur PBMC yang distimulasi oleh antigenEC610 pada kelompok TB aktif dan TB laten. Penelitian dan pemeriksaan dilakukan di RSK Paru Provinsi Sumsel danLaboratorium Pusat Penelitian dan Pengembangan Kesehatan, Jakarta Pusat. Terdapat 21 subjek penderita TB aktif dan28 subjek penderita TB laten yang sesuai dengan kriteria inklusi. Pengukuran kadar IFN-γ dan IL-10 dilakukanmenggunakan ELISA-Reader. Hasil penelitian menunjukkan kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610lebih tinggi pada TB aktif daripada TB laten. Tingginya kadar IFN-γ pada penderita TB aktif menunjukkan adanyarespon imun protektif terhadap kuman M.tb sedangkan tingginya kadar IL-10 menunjukkan perannya sebagai antiinflamasi . Tidak terdapat perbedaan bermakna rerata kadar IFN-γ pada penderita TB aktif dan TB laten (p=0,769) danterdapat perbedaan bermakna reata kadar IL-10 setelah stimulasi antigen EC610 lebih tinggi pada TB aktif daripada TBlaten (p=0,000)
IFN-γ and IL-2 Secretion after ESAT-6-CFP-10 (EC-610) Fusion Antigen Stimulation from Patients with Active Lung Tuberculosis and Latent Lung Tuberculosis
No full textThe Secretion of IFN-γ and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Activeand Latent TB Patients. This study held to discover how immune responses work and to know thepathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells withESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-γ and IL-2 with ELISA antibodysandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube withQuantiFERON for IFN-γ assay. The other four tubes were PBMC isolated using Ficoll-Paque, andpre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C andmeasured using T-Spot and ELISA reader. We got from this study that there are no significantdifferences in IFN-γ levels for both groups with active TB and latent TB. Measurement of IL-2 levelsshowed significant differences between the two group
Effectivity of Jatropha multifida L. Leaves Extract as Antibacterial on Streptococcus mutans using In Vitro Testing Methods
No full textIntoduction: Health is the condition of the entire body and body parts of an individual who is free from disease both physically, mentally and socially, thus enabling the individual to be able to carry out daily activities more productively. However, many people neglect their own health, especially in the field of dental and oral health. Caries is one of the most common oral and dental problems. The prevalence of dental caries in Indonesia is high, namely 88.8%. Dental caries is caused by Streptococcus mutans bacteria which plays a role in the conversion of sucrose to lactic acid. Infection due to Streptococcus mutans bacteria can be treated by taking antibiotics. Apart from drugs, antibiotics can also be obtained from natural ingredients, one of which is the tintir castor plant (Jatropha multifida Linn) or commonly known as the betadine.
Objective: To see the effect of the antibacterial content of tintir leaves against Streptococcus mutans bacteria and to determine the differences in the antibacterial effect of betadine leaf extract (Jatropha multifida L.) from a concentration of 25%, 50%, 75%, and 100% against Streptococcus mutans.
Methods: The type of research used in this study was a laboratory experimental study with a post-test-only control group design as the research design and to test the sensitivity of bacteria using the paper disc diffusion test. Data analysis using One-Way ANOVA.
Result: The results of this study indicate the effect of tintir castor leaf extract (Jatropha multifida L.) in inhibiting the growth of Streptococcus mutans bacteria
The Formulation and Physical Evaluation Tests of Ethanol in Telang Flower (Clitoria ternatea L.) Extract Losio Form as Antioxidant
No full textBackground. Antioxidants are defined as compounds that are able to delay, slow down, or inhibit oxidation reactions. One of the plants that has the potential as natural antioxidants is the telang flower. The purpose of this study was to determine whether the ethanol extract of telang flower (Clitoria ternatea L.) can be formulated in a losion form that meets the requirements of the physical evaluation test and to determine the ethanol extract of telang flower (Clitoria ternatea L.) has antioxidant activity.
Methods. The extraction using maceration techniques with 96% ethanol solvent. The yield obtained from the maceration technique was 29.16%. Phytochemical analysis of telang flowers contains alkaloids, flavonoids, polyphenols, saponins and tannins. Determination of the levels of flavonoids obtained levels of 10.05% and for polyphenols of 15.33%.
Results. Telang flower extract (Clitoria ternatea L.) can be formulated into losions and meets the requirements of the physical evaluation test in the extract concentration of 0.1%. The losion of telang flower extract (Clitoria ternatea L.) with a concentration of 0.1% telang flower extract has very strong antioxidant activity because it has an IC50 value <50 ppm, and the IC50 yield of a losion with a concentration of 0.1% telang flower extract (Clitoria ternatea L.) of 37.92 ppm which means that it has very strong antioxidant activity.
Conclusion. The formula of the telang flower extract lotion has strong antioxidant activity and protects the skin from free radicals