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Currículos alternativos para alunos com necessidades educativas especiais : 2º e 3º Ciclo
No full textp. 131-14
Differences In Phenotype, Growth Factor Requirements, Pattern Of Expression Of Adhesion Molecules And Rate Of Apoptosis Displayed By Three New Myeloid Sister Leukemic Cell Lines
No full text
Algumas reflexões sobre o trajecto escolar e as atitudes face à escola do grupo étnico cigano residente no Bairro S. João de Deus e em Espinho
No full textp. 27-4
Critical Evaluation of Techniques to Detect and Measure Cell Death – Study in a Model of UV Radiation of the Leukaemic Cell Line HL60
No full textThe reliability of eight distinct methods (Giemsa staining, trypan blue exclusion, acridine orange/ethidium bromide (AO/EB) double staining for fluorescence microscopy and flow cytometry, propidium iodide (PI) staining, annexin V assay, TUNEL assay and DNA ladder) for detection and quantification of cell death (apoptosis and necrosis) was evaluated and compared. Each of these methods detects different morphological or biochemical features of these two processes. The comparative analysis of the 8 techniques revealed that AO/EB (read in fluorescence microscopy) provides a reliable method to measure cells in different compartments (or pathways) of cell death though it is very time consuming. PI staining and TUNEL assay were also sensitive in detecting very early signs of apoptosis, but do not allow precise quantification of apoptotic cells. These three methods were concordant in relation to induction of apoptosis and necrosis in HL60 cells with the various UV irradiation time periods tested. Both AO/EB (read by flow cytometry) and annexin V‐FITC/PI failed to detect the same number of early apoptotic cells as the other three methods. Trypan blue is valueless for this purpose. Giemsa and DNA ladder might be useful as confirmatory tests in some situations
