Kochi University Repository

刈谷, 三郎

宮本, 隆信

Saburo, KARIYA

Takanobu, MIYAMOTO

The Attribution Analysis about the Improvement in Physical Fitness

柴, 英里

Eri, SHIBA

Evaluation of Picture Books for Shokuiku, Applying American Coloring Books, by Nursery school Staff

林, 翠芳

多義語「打」の諸表現に関する一考察　－日中両語の比較を通してー

庵地, 孝嗣

田村, 賢司

降旗, 睦夫

佐竹, 宏文

佐古田, 初音

川田, 千明

亀田, 麻依子

島本, 力

福原, 秀雄

藘田, 真吾

辛島. 尚

西川, 宏志

山崎, 一郎

蒲田, 雅行

井上, 啓史

執印, 太郎

SNRPEはARの発現制御を通して前立腺癌の増殖や進展に関与する

西窪,加緒里

咽喉頭の温度感覚受容体刺激による嚥下障害に対する薬物治療の開発

Fabricio M., Locatelli

Juan J., Lauthier

Jorge D., Marco

Paola A., Barroso

Keiko, Morisawa

Miguel A., Basombrio

Yoshihisa, Hashiguchi

Masataka, Korenaga

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30.Evaluation of two new targets for MLST in Leishmania
Fabricio M. Locatelli 1), Juan J. Lauthier 2), Jorge D. Marco 2), Paola A. Barroso 2), Keiko Morisawa 3),
Miguel A. Basombrío 2), Yoshihisa Hashiguchi 1), 4), Masataka Korenaga 1).
1) Department of Parasitology, Kochi Mededical School, Kochi University,  
2) Institute of Experimental Pathology, National University of Salta-CONICET, Argentina,
3) Science Research Center, Kochi University, 4) PROMETEO, Ecuador
      Leishmaniasis is a protozoal disease caused by parasites of the genus Leishmania.  The clinical 
manifestation goes from the less sever forms (cutaneous leishmaniasis) to the visceral form which is severer and 
can be deathly if remained without treatment. Several different species of Leishmania have been 
described throughout South America. In Argentina two species, Leishmania (V.) brazilensis and L. (L.) 
infantum, have recently emerged. Because of the high variability and diversity in this genus, it is necessary to 
have an accurate method for typing. Multilocus Enzyme Electrophoresis (MLEE) is the reference method 
suggested by the WHO for identification and typing of Leishmania. However, this technique is laborious and 
time-consumable, and also different allozyme may have coincidental mobility.  DNA analysis methods, therefore, 
have been proposed without the disadvantages mentioned. These methods stand out Multilocus Sequence Typing 
(MLST), which originally was proposed for bacteria typing but recently have been used 
for Leishmania and other diploid organisms. The first MLST scheme proposed is difficult to accomplish, 
because it is necessary to develop a new strategy with new targets much shorter and capable to identify the 
different species of Leishmania. In this work we evaluated two new targets, Aspartate Aminotransferase (ASAT) 
and 6 Phosphogluconate Dehidrogenase (6PGD). Two primers were designed for each gene, which amplified a 
sequence of 877bp for ASAT and 700 bp for 6PGD. Both genes were tested over 16 samples (8 reference strains 
and 8 strains from Argentina). 
Conclusion: Both genes PGD and ASAT showed considerable polymorphism and a high power of discrimination, 
which made them excellent candidates for Leishmania isolates typing. 


Evaluation of two new targets for MLST in Leishmania

石田, わか

福田, 憲

楮佐古, 美奈

坂本, 修士

八木田, 秀雄

福島, 敦樹

実験的自己免疫性ぶどう膜炎発症におけるDelta-like ligand4 の関与

堀池, 諒

潤井, 秀枝

栗林, 一恵

長谷, 佳織

高尾, 俊弘

医学部学生における食行動とストレスの関連

沈, 淵

都留, 英美

矢生, 健一

王, 飛霏

江端, 千尋

津田, 雅之

前田, 長正

マウスの新しい低酸素性虚血性脳症モデルの樹立及び末梢血幹細胞を用いた治療法の検討

倉林, 睦

前立腺癌に対するphotodynamic therapy

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