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銀ナノ粒子はヒト線維芽細胞様肺細胞株と歯根膜線維芽細胞に対しミトコンドリア機能の変化を介してACE2発現を減少させる
Full text link鹿児島大学博士(歯学)Doctor of Philosophy in Dental Science博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨Silver nanoparticles (AgNPs) have demonstrated antibacterial properties and are widely recognized as one of the most prominent types of nanoparticles. Recent studies have highlighted their effectiveness against coronaviruses. However, the detailed molecular mechanisms underlying the action of AgNPs on viruses and their impacts on the human body remain to be fully elucidated. Thus, we attempt to delineate the preventive effects of AgNPs against SARS-CoV-2 infection. Our findings indicate that treatment with AgNPs reduces ACE2 expression, a key receptor for SARS-CoV-2 particularly in normal oral and lung cells. Additionally, we observed a decrease in the binding affinity of the spike protein to the cell after the AgNP treatment. Through western blot analysis, we identified the involvement of the AKT and/or mTOR signaling pathway in this process. Because AKT and mTOR signaling affects mitochondrial function, we investigated the effects of the AgNP treatment on mitochondria. As a result, we observed the localization of AgNPs within mitochondria. Further, an increase in mitochondrial Fe2+ and reactive oxygen species levels was observed, ultimately resulting in mitochondrial dysfunction. Our results show the efficacy of the AgNP treatment in preventing coronavirus infections.Shosei Takahashi, Kazuo Tomita, Kento Igarashi, Yoshikazu Kuwahara, Junichi Kitanaka, Nobue Kitanaka, Koh-ichi Tanaka, Akihiro Kurimasa, Yoshiaki Kamikawa & Tomoaki Sato
Silver Nanoparticles Reduce ACE2 Expression via Changing Mitochondrial Function in Human Fibroblast-Like Lung Cell and Periodontal Ligament Fibroblast Cells
BioNanoSci. 15, 39 (2025)
https://doi.org/10.1007/s12668-024-01630-7The version of record of this article, first published in BioNanoScience, is available online at Publisher’s website: https://doi.org/10.1007/s12668-024-01630-7doctoral thesi
歯科診療中の自律神経活動の解析
Full text link鹿児島大学博士(歯学)Doctor of Philosophy in Dental Science博士論文要約, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨thesi
癌抑制型miRNA (miR-30c-1-3p, miR-30c-2-3p)が制御する癌遺伝子TRIP13は乳癌の攻撃性を促進する
Full text link鹿児島大学博士(医学)Doctor of Philosophy in Medical Science博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨Accumulating evidence suggests that the miR-30 family act as critical players (tumor-suppressor or oncogenic) in a wide range of human cancers. Analysis of microRNA (miRNA) expression signatures and The Cancer Genome Atlas (TCGA) database revealed that that two passenger strand miRNAs, miR-30c-1-3p and miR-30c-2-3p, were downregulated in cancer tissues, and their low expression was closely associated with worse prognosis in patients with BrCa. Functional assays showed that miR-30c-1-3p and miR-30c-2-3p overexpression significantly inhibited cancer cell aggressiveness, suggesting these two miRNAs acted as tumor-suppressors in BrCa cells. Notably, involvement of passenger strands of miRNAs is a new concept of cancer research. Further analyses showed that seven genes (TRIP13, CCNB1, RAD51, PSPH, CENPN, KPNA2, and MXRA5) were putative targets of miR-30c-1-3p and miR-30c-2-3p in BrCa cells. Expression of seven genes were upregulated in BrCa tissues and predicted a worse prognosis of the patients. Among these genes, we focused on TRIP13 and investigated the functional significance of this gene in BrCa cells. Luciferase reporter assays showed that TRIP13 was directly regulated by these two miRNAs. TRIP13 knockdown using siRNA attenuated BrCa cell aggressiveness. Inactivation of TRIP13 using a specific inhibitor prevented the malignant transformation of BrCa cells. Exploring the molecular networks controlled by miRNAs, including passenger strands, will facilitate the identification of diagnostic markers and therapeutic target molecules in BrCa.Mitsueda, R.; Toda, H.; Shinden, Y.; Fukuda, K.; Yasudome, R.; Kato, M.; Kikkawa, N.; Ohtsuka, T.; Nakajo, A.; Seki, N.
Oncogenic Targets Regulated by Tumor-Suppressive miR-30c-1-3p and miR-30c-2-3p: TRIP13 Facilitates Cancer Cell Aggressiveness in Breast Cancer
Cancers 2023, 15, 4189
https://doi.org/10.3390/cancers15164189doctoral thesi
センチネルリンパ節と非センチネルリンパ節における樹状細胞とマクロファージの分布の相違について : 早期胃癌に対するセンチネルリンパ節ナビゲーション手術で採取した領域リンパ節を用いた免疫組織染色及び形態学的研究
Full text link鹿児島大学博士(医学)Doctor of Philosophy in Medical Science博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨The sentinel lymph node (SN) concept has a significant impact on cancer surgery. We aimed to examine which morphology of dendritic cells (DCs) and macrophages corresponds to “preconditioning” of the SN against cancer. Although macrophages are generally able to tolerate cancer metastasis, the CD169-positive subtype is believed to be a limited exception. Immunohistochemical and morphometric analyses were performed to examine DC-SIGN-, CD68-, and CD169-positive cells in SNs and non-SNs of 23 patients with gastric cancer with or without nodal metastasis. All patients survived for >5 years without recurrence. DCs were present in the subcapsular, paracortical, and medullary sinuses, the endothelia of which expressed DC-SIGN and smooth muscle actin (SMA). In the non-SNs of patients without metastasis, subcapsular DCs occupied a larger area than SNs, and this difference was statistically significant. Conversely, subcapsular DCs were likely to have migrated to the paracortical area of the SNs. DC clusters often overlapped with macrophage clusters; however, histiocytosis-like clusters of CD169-negative macrophages showed a smaller overlap. We found a significantly larger overlap between DC-SIGN and CD169-positive clusters in SNs than in non-SNs; the larger overlap seemed to correspond to a higher cross-presentation of cancer antigens between these cell populations. DC-SIGN–CD169-double positive cells might exist within this overlap. SNs in gastric cancers are usually preconditioned as a frontier of cancer immunity, but they may sometimes be suppressed earlier than non-SNs. DC-SIGN- and CD169-positive cells appeared to decrease owing to a long lag time from the primary lesion occurrence and a short distance from the metastasis.Tomohiro Sonoda, Takaaki Arigami, Masaya Aoki, Daisuke Matsushita, Masataka Shimonosono, Yusuke Tsuruda, Ken Sasaki, Takao Ohtsuka, Gen Murakami
Difference between sentinel and non-sentinel lymph nodes in the distribution of dendritic cells and macrophages: An immunohistochemical and morphometric study using gastric regional nodes obtained in sentinel node navigation surgery for early gastric cancer
Journal of Anatomy. 2025;246:272–287
https://doi.org/10.1111/joa.14147doctoral thesi
重症心身障がい入院患者における栄養状態予測のための多周波生体電気インピーダンス分析の臨床応用可能性の評価
Full text link鹿児島大学博士(保健学)Doctor of Philosophy in Health Science博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨Background: Persons with severe motor and intellectual disabilities (SMID) have difficulty using general nutritional assessments in the elderly and other populations due to their physical characteristics. The purpose of this study was to investigate the clinical utility of body composition, which has been suggested to be related to biochemical tests in persons with SMID
Methods: We calculated cutoff values of body composition predicting malnutrition and compared the usage of peripheral parenteral nutrition for the two groups divided by each body composition cutoff value. We also compared body composition at the baseline between non-survivors and survivors.
Results: Only group comparisons based on skeletal muscle ratio and extracellular water to total body water (ECW/TBW) cutoffs showed significant differences in the usage of peripheral parenteral nutrition. Non-survivors had significantly higher ECW/TBW than survivors at the baseline.
Conclusions: In persons with SMID, skeletal muscle ratio, and ECW/TBW measured using multifrequency bioelectrical impedance were associated with biochemical indices and ill health. Furthermore, ECW/TBW was also associated with mortality, suggesting that ECW/TBW is a significant measure for nutritional assessment in clinical practice.This is the peer reviewed version of the following article: Nozomu Yano, Akihiko Ohwatashi, Daishi Iwashita; Evaluating the Clinical Applicability of Multifrequency Bioelectrical Impedance Analysis for Nutritional Status Prediction in Hospitalized Persons With Severe Motor and Intellectual Disabilities; Journal of Intellectual Disability Research; 2024; 69(3),204-213, which has been published in final form at https://doi.org/10.1111/jir.13203. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.doctoral thesi
A study on antimicrobial resistance of pathogenic Escherichia coli isolated from livestock in Kagoshima prefecture
Full text link鹿児島大学博士(獣医学)博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨doctoral thesi
Studies on Change of the Transmission Modes and of the Expressive Forms in Amami Shima-uta : Focusing on the Shimauta School
Full text link鹿児島大学博士(学術)Doctor of Philosophy博士論文要約, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨thesi
Poverty and Ethnic Tourism in China : An Analysis of Hosts, Guests, and Intermediaries in Tujia and Miao Regions
Full text link鹿児島大学博士(学術)Doctor of Philosophy博士論文要約, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨thesi