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Chemical exploration of different extracts from Phytolacca americana leaves and their potential utilization for global health problems: ın silico and network pharmacology validation
No full textPhytolacca americana L. is of great interest as a traditional additive in various folk remedies in several countries, including Turkey. We aimed to determine the chemical profile (assisted by high-Performance liquid chromatography-electrospray ionization-tandem mass apectrometry (HPLC-ESI-MS/MS) experiments of three extracts obtained by different polarity solvents viz. ethyl acetate (to extract semipolar compounds), methanol and water (to extract highly polar metabolites) from P. americana leaves. Their anti-diabetic effects were investigated in vitro by assessing their inhibition toα-amylase and α-glucosidase. Assessment of the neuroprotective potential of the three extracts was carried out against acetyl-(AChE) and butyryl-(BChE) cholinesterase enzymes. HPLC-ESI-MS/MS experiments showed a total of 17 chromatographic peaks primarily classified to six flavonoids, two saponins, and six fatty acids. Antioxidant assays revealed remarkable activity for the ethyl acetate and methanol extracts. The BChE inhibition was considerably more significant (4.08 mg galantamine equivalent (GALAE)/g) for the ethyl acetate extract, whereas the methanol extract had good inhibitory efficacy for AChE (2.05 mg GALAE/g). Through network pharmacology, the compounds’ mechanism of action of targeted key gene in their associated diseases were identified. The hubb gene signal transducer and activator of transcription 3 (STAT3) and tumour necrosis factor (TNFα) where the P. americana compound’s site of action in inflammation bowel disease. The results offer possibilities for the prospective application of P. americana in metabolic regulation, blood glucose control, and as a source of bioactive compounds with cholinesterase enzyme inhibitory characteristics which could be of relevance in the cosmetic or pharmaceutical industry for combating melanogenesis. Communicated by Ramaswamy H. Sarma. © 2024 Informa UK Limited, trading as Taylor & Francis Group
Assessing the Chemical Profile and Biological Potentials of Tamarix aphylla (L.) H.Karst. and Tamarix senegalensis DC. by In Vitro, In Silico, and Network Methodologies
No full textThe present study aimed to investigate the chemical profile, antioxidant, and enzyme inhibition properties of extracts from fruits and aerial parts (leaves and twigs) of Tamarix aphylla and T. senegalensis. Hexane, dichloromethane, ethyl acetate (EtOAc), and methanol extracts were prepared sequentially by maceration. Results revealed that EtOAc extracts of T. senegalensis and T. aphylla fruits contained the highest total phenolic content (113.74 and 111.21 mg GAE/g) while that of T. senegalensis (38.47 mg RE/g) recorded the highest total flavonoids content. Among the quantified compounds; ellagic, gallic, 3-hydroxybenzoic, caffeic, syringic, p-coumaric acids, isorhamnetin, procyanidin B2, and kaempferol were the most abundant compounds in the two species. EtOAc extracts of the two organs of T. senegalensis in addition to MeOH extract of T. aphylla aerial parts displayed the highest chelating power (21.00-21.30 mg EDTAE/g, p > 0.05). The highest anti-AChE (3.11 mg GALAE/g) and anti-BChE (3.62 mg GALAE/g) activities were recorded from the hexane and EtOAc extracts of T. senegalensis aerial parts and fruits, respectively. EtOAc extracts of the fruits of the two species exerted the highest anti-tyrosinase (anti-Tyr) activity (99.44 and 98.65 mg KAE/g, p > 0.05). Also, the EtOAc extracts of the both organs of the two species exhibited highest anti-glucosidase activity (0.88-0.90 mmol ACAE/g, p > 0.05) while the best anti-alpha-amylase activity was recorded from the dichloromethane extract of T. senegalensis fruits (0.74 mmol ACAE/g). In this study, network pharmacology was employed to examine the connection between compounds from Tamarix and their potential effectiveness against Alzheimer's disease. The compounds demonstrated potential interactions with pivotal genes including APP, GSK3B, and CDK5, indicating a therapeutic potential. Molecular docking was carried out to understand the binding mode and interaction of the compounds with the target enzymes. Key interactions observed, such as H-bonds, promoted the binding, and weaker ones, such as van der Waals attractions, reinforced it. These findings suggest that these two Tamarix species possess bioactive properties with health-promoting effects
Energy Absorption of a Novel Lattice Structure-Filled Multicell Thin-Walled Tubes Under Axial and Oblique Loadings
No full textMulticell design and lattice structure as filling material are two effective methods for enhancing the energy absorption performance of thin-walled tubes. This study combines these two approaches to present a multicell tube with a novel lattice structure and investigates the energy absorption performances of these hybrid multicell tubes under axial (0 degrees) and oblique (10 degrees, 20 degrees, and 30 degrees) impact loading conditions. As filling structure, beta-Ti3Au lattice geometry with varying lattice strut diameters and the number of lattice unit cells are used, while the single and multicell thin-walled tubes with different tube thicknesses are employed as main absorbing element. In this context, the effects of numbers of lattice unit cells, lattice strut diameter, cell numbers of the tube, and tube thickness on energy absorption performance of hybrid tubes are examined using validated nonlinear finite element models. This investigation unveils that the synergistic interplay between the multicell tubes and lattice structure during deformation significantly elevates the energy absorption performance of the hybrid structure. Notably, the findings demonstrate that multicell hybrid tubes exhibit a remarkable capacity to absorb up to 30.36% more impact energy compared to the aggregate absorption of individual components in hybrid tubes.This study introduces the beta-Ti3Au lattice structure as a novel filling material to enhance the energy absorption of thin-walled multicell tubes. It examines the effects of lattice unit cell numbers, lattice strut diameter, tube cell numbers, and tube thickness on energy absorption using validated nonlinear finite element models.image (c) 2024 WILEY-VCH Gmb
A helpful new technique in gynecomastia surgery
No full text– OBJECTIVE: The aim of this study was to elucidate the external temporary skin plication (ETSP) technique in gynecomastia surgery and evaluate its role in mitigating complications and enhancing cosmetic outcomes. PATIENTS AND METHODS: This study was conducted on patients diagnosed with gynecomastia, explicitly falling under Rohrich Grades IIB and III, with dermal quality being a crucial determinant. Between September 2018 and November 2021, surgical interventions were performed on 96 qualifying patients by the senior author. The operative protocol consisted of ultrasonic and suction-assisted liposuction, supplemented by lateral periareolar piecemeal gland excision. Within the cohort, 42 patients were subjected to the novel ETSP technique. In contrast, the remaining 54 patients underwent standard treatment, serving as the control group for subsequent comparative assessment. RESULTS: One patient required revisions for contour irregularities, while partial nipple necrosis was observed in two patients but healed without surgical intervention. However, saucer-like deformity and total nipple necrosis were not observed in our series. The overall complication rate in our series was 19%, with 9.5% of cases requiring revision. In our control group, the overall complication rate in our series was found to be 22%, with 13% of cases requiring revision. CONCLUSIONS: ETSP provides a homogeneous spread of the excess skin and greatly reduces or eliminates the amount of skin fold formation. ETSP reduces the need for possible skin excision and reduces visible scars and incisions, and it helps improve the results of skin-protective surgeries that are widespread today. © 2024 Verduci Editore s.r.l. All rights reserved
The Derivation of Vertical Damping Reduction Factors for the Design and Analysis of Structures Using Acceleration, Velocity, and Displacement Spectra
No full textDamping reduction factors (DRFs) play a vital role in the seismic design of structures. DRFs have been widely studied due to their primary importance to the lateral resistance of structures subjected to earthquakes. On the other hand, devastating earthquakes have occurred all over the world, and recently, the Kahramanmara & scedil; earthquakes in Turkey revealed the import of the vertical component of earthquakes and their impact on structures and infrastructures. Considering the importance of this parameter, this paper aims to develop new damping reduction factor (DRF) equations for the acceleration (DRFa), velocity (DRFv), and displacement spectra (DRFd) of the vertical components of earthquakes. For this purpose, 775 real ground motion records were selected from the Pacific Earthquake Engineering Research (PEER) strong motion database, and the vertical elastic response spectra of selected records were computed according to linear dynamic analysis. Taking the 5%-damped vertical response spectra as the target, the vertical spectral damping reduction factors (DRFa, DRFv, and DRFd) were computed for 1%, 3%, 10%, 15%, 20%, 30%, and 40% damping ratios. The effect of the earthquake magnitude, distance, and soil types on the DRFs was investigated. The results indicated that magnitude, distance, and soil type had no particular effect on the trend in the DRFs. Based on the evaluations, extensive statistical analyses were carried out, and new prediction equations were developed according to the nonlinear regression method. The developed equations were then compared to those found in the literature and seismic design codes. The comparisons proved that the proposed DRFa, DRFd, and DRFv models are strongly compatible with real DRFs and show strong robustness compared to existing models
An Exploratory Study
No full textIn recent times, the rise of urbanization, industrialization, population growth, food security, and the COVID-19 pandemic have led to an increased demand for indoor spaces with efficient air conditioning systems. As a result, there is a growing interest in creating more complex HVAC systems to improve indoor spaces. Building information modeling (BIM) offers numerous benefits to the HVAC industry, such as clash detection, budget and time reductions, and increased efficiency. However, its implementation is currently hindered by various challenges. This research aims to identify the major barriers to BIM implementation in the HVAC industry in Turkey, using a questionnaire survey of 224 domain experts working in 42 different companies across various fields of the HVAC industry. The study utilized several statistical analyses to categorize and prioritize the most critical barriers, including reliability tests, exploratory factor analysis (EFA), confirmatory factor analysis (CFA), the Kaiser-Meyer-Olkin (KMO) test, Bartlett's test, and ranking of factors (IRI). The results indicate that the "Deficiencies of Infrastructure and Lack of Qualified Personnel (DIP)" factor group constituted the most significant barrier, followed by "Lack of Documentation and Specifications (LDS)", "Deficiencies of Case Studies and Project Drawings (DCP)", and "Lack of Motivation and Resistance to BIM (LMR)". Moreover, our research revealed that 60% of the participants' companies allocate less than 40% of their budgets to technological infrastructure, which hinders the adoption of BIM. To promote BIM in the HVAC sector, we recommend enhancing personnel capacity building, improving skills and knowledge about BIM, promoting guidelines, and providing free access to documentation for practitioners
Biological, computational evaluation of novel benzofuranyl derivatives as acetylcholinesterase and butyrylcholinesterase
No full textAim: A highly efficient one-step method has been developed for the synthesis of benzofuranyl derivatives from 2-benzoylcyclohexane-1-carboxylic acid derivatives using chlorosulfonyl isocyanate. This novel method provides a practical, cost-effective and efficient approach. Materials & methods: The inhibitory effects of benzofuranyl derivatives on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were investigated. Ki values were determined to range from 0.009 to 0.61 mu M for AChE and 0.28 to 1.60 mu M for BChE. Molecular docking analysis provided insights into the interaction modes and binding patterns of these compounds with AChE and BChE. Conclusion: Kinetic findings of our study suggest that some of our compounds exhibited more effective low micromolar inhibition compared with the reference, and these derivatives could be to design more powerful agents
miR-3653-3p Expression in PBMCs: Unveiling the Diagnostic Potential for Ovarian Cancer
No full textOvarian cancer is typically diagnosed at an advanced stage, recurs early and often, and currently lacks effective treatment. Therefore, overall survival and progression-free survival are relatively short for this disease. Sensitive and specific biomarkers for early diagnosis and follow-up for effective treatment of the disease are currently lacking. MicroRNA (miRNA/miR) expression studies are widely used in cancer research. Disruption or malfunction of miRNAs, a class of noncoding small RNAs, has been implicated in cancer progression in several publications. Of note, the expression of a series of miRNAs is known to differ in ovarian cancer. In cancer research, it is crucial to analyze expression patterns in both cancer patients and healthy individuals to identify cancer-specific biological markers and to understand their role in cancer. In the present study, the expression levels of miR-3653-3p in the peripheral blood mononuclear cells (PBMCs) of 150 patients with high-risk ovarian cancer were determined, including those with a family history of cancer or an early-age diagnosis of ovarian cancer, as well as 100 healthy individuals. The results were then compared between the two groups. The expression level of miR-3653-3p in the PBMCs of patients with ovarian cancer was determined to be 9.49-fold higher than that in the healthy control group, and this result was statistically significant (P < 0.001). In addition, receiver-operating characteristic curve analysis of PBMC showed statistical significance of miR-3653-3p in discriminating ovarian cancer patients from healthy subjects (P < 0.001). These results suggest that miR-3653-3p detected in peripheral blood may be used as a non-invasive biomarker for ovarian cancer
Combining chemical profiles and biological abilities of different extracts from Tanacetum nitens ( Boiss. & NoE) Grierson using network pharmacology
No full textTanacetum nitens ( Boiss. & No & euml;) Grierson is an aromatic perennial herb used in Turkish traditional medicine to treat headache, fever, and skin diseases. This study aimed to investigate the chemical composition, antioxidant, enzyme inhibition, and cytotoxic properties of T. nitens aerial parts. Organic solvent extracts were prepared by sequential maceration in hexane, dichloromethane, ethyl acetate, and methanol while aqueous extracts were obtained by maceration or infusion. Nuclear magnetic resonance (NMR) and LC-DAD-MS analysis allowed the identification and quantification of different phytoconstituents including parthenolide, tanacetol B, tatridin B, quinic acid derivatives, beta-sitosterol, and glycoside derivatives of quercetin and luteolin. The type and amount of these phytochemicals recovered by each solvent were variable and significant enough to impact the biological activities of the plant. Methanolic and aqueous extracts displayed the highest scavenging and ions-reducing properties while the dichloromethane and ethyl acetate extracts exerted the best total antioxidant activity and metal chelating power. Results of enzyme inhibition activity showed that the hexane, ethyl acetate, and dichloromethane extracts had comparable anti-acetylcholinesterase activity and the latter extract revealed the highest anti-butyrylcholinesterase activity. The best alpha-amylase and alpha-glucosidase inhibition activities were obtained from the hexane extract. The dichloromethane and ethyl acetate extracts exhibited the highest cytotoxic effect against the prostate carcinoma DU-145 cells. In conclusion, these findings indicated that T. nitens can be a promising source of biomolecules with potential therapeutic applications
The Moderated Mediation Model
No full textLife satisfaction is about evaluating life quality. COVID-19 fear affecting individuals’ well-being might cause negativities. COVID-19 fear and life satisfaction were examined with many variables, such as distress, well-being, and mental health. Focusing on the social work perspective, this study aims to examine COVID-19 fear’s indirect effect on life satisfaction through economic anxiety, which is important for refugees and not previously considered. It also aims to examine how this indirect effect varies depending on the age. Between July 2021 and June 2022, 615 Syrian refugees from Turkey participated in this cross-sectional study. This study used the Hayes’ PROCESS macro. The results showed that the COVID-19 fear’s negative indirect effect on life satisfaction through economic anxiety is statistically significant. Furthermore, moderated mediation analysis revealed that the negative indirect effect varies depending on the refugees’ age, and this effect becomes stronger as age increases. Therefore, policymakers need to consider the age and situations that may cause economic anxiety, such as uninsured employment and easy dismissal refugees often encounter, in social work policies. In future studies, different variables related to the crisis might be discussed within various perspectives on similar crises. © 2024 Taylor & Francis Group, LLC