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Prenatal gender selection: medical, ethical and psychological aspects
Get PDFThe aim: to summarize the methodology of prenatal gender selection and the ethical aspects of its implementation.
Materials and methods: the authors conducted the review in the database of scientific periodicals PubMed.
Results: based on scientific publications, the principal techniques to determine sex in the pre-implantation period, in the embryo and fetus, are presented. Invasive and non-invasive procedures of prenatal diagnosis are described. Invasive procedures include examination of the fetus by chorionic villus sampling karyotyping of cells obtained by amniocentesis. Non-invasive techniques related to pregnant women's blood biochemistry and ultrasound markers of Down syndrome are given. Fetal blood DNA testing is a more sensitive diagnostic parameter that reduces the indication for invasive procedures. The newest and most effective method of sex determination in the pre-implantation period is sperm separation screening. Medical and non-medical reasons for prenatal gender determination, such as prevention of X-linked genetic disorders in a child, a couple's choice to balance the sex of children in the family and social gender preferences are shown. Gender selection for the prevention of X-linked disorders is justified. Ethical issues regarding the balance of children in the family, giving preference to certain sex with the help of advanced diagnostic techniques, and selective abortion are discussed. The bioethical principles for preventing selective abortion resulting from gender reasons are established.
Conclusion: for preventing prenatal gender discrimination is highlighted more strongly by the need legal regulation and the ethical control of scientific technologies in reproductive medicine
A cross-sectional study to assess proteinuria and lipoprotein (a) levels in chronic kidney disease
Get PDFChronic kidney disease (CKD) is a reduced glomerular filtration rate and/or increased urinary albumin excretion. The worldwide prevalence of chronic kidney disease (CKD) ranges from 8 to 16 %, and the prevalence of CKD is rising.
The aim: To study the association between CKD stages, proteinuria, and lipoprotein (a) levels among the study participants.
Materials and methods: This study was an institution-based observational case-control study involving CKD patients as study group and healthy volunteers as control one. Blood samples were tested for urea, serum creatinine, uric acid levels, triglycerides, total cholesterol, HDL cholesterol, VLDL cholesterol and serum lipoprotein. Statistical analysis was done with SPSS version 20.0.
Result: In our study, the most common age group affected among cases was 41 to 50 years (5th decade), and there was a male preponderance in CKD. CKD patients had a higher mean protein creatinine ratio than controls, and this difference was statistically significant. In addition, CKD patients had significantly higher total cholesterol, triglyceride levels and lower HDL cholesterol levels than controls. Also, they had significantly elevated serum lipoprotein (a) levels than controls.
Conclusion: Based on our study findings, we can conclude that because of the potential role of lipoprotein (a) in the development of cardiovascular disease, it is imperative to include an estimation of lipoprotein (a) levels in all CKD patients, especially in later stages to give a targeted therapy for dyslipidemia among CKD patient
Postpartum haemorrhage: minimum allowable haemoglobin level
Get PDFDespite defining the acceptable postpartum haemoglobin levels, there is neither consensus, nor a strong statistically proven analysis of its minimum safe level and its influence on systemic haemodynamic and oxygen metabolism in particular.
The aim. Therefore, this study aimed to determine statistically minimal allowable haemoglobin levels in postpartum women in case of postpartum haemorrhage which allows the cardiac functional status and oxygen metabolism to be maintained on the lower margin of the physiological range.
Materials and methods: Clinical research was on 113 postpartum women who had received blood transfusions for postpartum haemorrhages were selected for this study. The following circulation parameters were assessed: heart rate (HR), systolic blood pressure (BPs), diastolic blood pressure (BPD), mean arterial pressure (MAP), central venous pressure (CVP), cardiac index (СІ), contractility index (ΔS), systemic vascular resistance index (SVRI), systemic oxygen delivery index (IDО2), systemic oxygen consumption index (IVО2), tissue oxygen extraction ratio (O2ER).
Results: Through a comparison of hemodynamic parameters depending on haematocrit and haemoglobin levels in patients with postpartum blood loss it was discovered that in case of blood loss and consequent intensive therapy aimed at TBV (total blood volume) restoration statistically significant changes of BPs, MAP, СІ and SVRI were observed. Also, during this study linear high-degree correlations between СІ, ΔS and Hb levels were found.
Analysis of systemic oxygen transport dependence on haematocrit and haemoglobin levels for patients during the early postpartum period demonstrates significant differences in cardiac indexes with a strong positive statistically significant correlation between these parameters and haemoglobin levels.
Conclusion. From the calculation of the minimum allowable haemoglobin level in postpartum women in case of blood loss using linear regression with coefficients calculated through the method of least squares the Hb=82.5 g/L value was obtained, which can be considered the minimum allowable level in postpartum women in case of postpartum blood loss which allows the cardiac functional status and oxygen metabolism to be on safety physiological rang
Association between lichen planus and dyslipidemia: an experience from North India
Get PDFAssociation between lichen planus (LP) and dyslipidaemia and other cardiovascular risk factors has been reported in many studies in the past, with variable results between studies.
The aim: this study was undertaken to study the association of lichen planus with dyslipidaemia.
Methods: this was a prospective hospital-based case control study conducted over a period of three years, on 105 prospective newly diagnosed male patients of lichen planus and equal number of age and sex matched controls. Fasting serum lipid profile including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C) were measured, and comparison done between cases and controls.
Results: dyslipidaemia was found in 44 (41.9 %) lichen planus patients (cases) as compared to 28 (26.7 %) controls, the difference being statistically significance (p 0.020). Triglycerides, total cholesterol, LDL cholesterol and VLDL cholesterol were significantly higher in cases than controls, whereas difference in the values of abdominal circumference and body mass index (BMI), although more in cases than controls was not statistically significant. Similarly, HDL-cholesterol values were less in cases than controls, but without statistical significance.
Conclusion: dyslipidaemia was more common in lichen planus (LP) patients, as compared to controls, suggesting that LP patients are at a higher risk of developing derangements of serum lipids and should be routinely and regularly monitored for dyslipidaemia and other cardiovascular risk factors, to detect cardiovascular diseases well in time. The result of present study strengthens the evidence of association between LP and dyslipidaemia
Prevention of gastrointestinal diseases in piglets at weaning using probiotics Bacillus coagylans and Bacillus megaterium
Get PDFThe aim of the study is to determine the preventive effect of the use of probiotics Bacillus coagulans and Bacillus megaterium in case of gastrointestinal diseases in piglets and as a potential strategy to reduce the misuse of antibiotics in monogastric animals by adding probiotics to the diet of pregnant sows and piglets in the period before and after weaning.
Materials and methods. Three research groups were formed to study the preventive effect of probiotics of the genus Bacillus coagulans and Bacillus megaterium. The piglets of the first experimental group received probiotics at the rate of 0.4 kg per 1 ton of feed in the periods before and after weaning, and that is, the moment of the entire study, the second experimental group received probiotics in the period after weaning, before that they received milk from the sow. The third group was the control group, which received only milk from the sow, and fodder during the weaning period. The effectiveness of the prevention result was determined by the absence/presence of clinical signs of morbidity and % survival of animals during the study period.
The results. It was established that the preservation of livestock in the 1st experimental group was 90 %, and in the 2nd group – 70 %, and control – 50 %.
Conclusions. Research results show that probiotics of the genus Bacillus coagulans and Bacillus megaterium, the components of which provide a wide spectrum of antimicrobial activity, are a reliable preventive measure for gastrointestinal diseases in piglets
Prevalence of oral lichen planus among north Indian population: a descriptive epidemiological study
Get PDFThe aim: oral lichen planus is a chronic inflammatory disease affecting the oral mucous membrane. It can present in various clinical forms: reticular, papular, plaque-like, atrophic, erosive and bullous. Though the exact etiology is not certain - autoimmunity, trauma, stress, and habits are considered as etiological factors. Oral lichen planus has been classified as a premalignant lesion. The present study aims to describe the prevalence of oral lichen planus in a sample of north Indian population.
Materials and methods: the present study was conducted in the outpatient department of Dentistry, GMC Badaun. The parameters recorded were age, gender and site affected. The data was collected and analysed.
Results: a total of 6263 patients reported to the outpatient department of dentistry out of which 43 patients were diagnosed with oral lichen planus. The overall prevalence of oral lichen planus was found to be 0.69 %. The mean age of disease presentation was 37.5 years. A higher prevalence of oral lichen planus was reported in females compared to males with a ratio of 1:1.15. Most affected site was found to be buccal mucosa bilaterally (58.14 %).
Conclusion: the present study estimated the overall prevalence of oral lichen planus as well as its distribution according to age, gender and site in north Indian population. This could contribute to the precise assessment of the disease for better policy making for better treatment and management of oral lichen planus
Study of the laser radiation effect in combination with doxorubicin on the survival of MCF7 and MCF7DOX culture cells
Get PDFThe use of lasers in oncology is constantly evolving. In recent years, the mechanisms of the effect of laser radiation, particularly of the infrared spectrum, on tumour cells in combination with various chemotherapeutic agents have been studied.
The aim. To study the effect of laser radiation with a wavelength of 660 and 810 nm on the survival of tumour cell cultures MCF7 and MCF7DOX in the presence of doxorubicin.
Materials and methods. Tumour cells of MCF7 and MCF7DOX cultures were cultured in DMEM medium (Biowest, France) with 10 % fetal calf serum (FST) (Biowest, France) and 40 μg / ml gentamicin (Sigma, USA). Doxorubicin was added to the cells to a final concentration of 1 μg / ml. Cells were irradiated with a laser (Photonica-Plus, Ukraine) with a wavelength of 660 and 810 nm (irradiation time – 5 min, power density – 50 mV/cm2, irradiation dose 15 J/cm2). The results were recorded using a multiwell spectrophotometer (Labsystems Multiskan PLUS, Finland). Photomicrographs of cells were taken using a Carl Zeiss microscope, Germany.
Results. Cell survival assessment and morphological characteristics in micropreparations indicate the antitumor efficacy of the combined effects of laser irradiation and doxorubicin. The most pronounced cytotoxic effect on MCF7-DOX culture cells was caused by doxorubicin exposure for 90 min in combination with infrared laser irradiation (λ=810 nm).
Conclusions. Infrared laser light synergises with the toxic effects of doxorubicin and creates favourable conditions for apoptosis of tumour cells, as evidenced by cytomorphological data
The possibility of using anti-human monoclonal antibody CD3 as pan T-cell marker in guinea pigs
Get PDFThe present study was aimed to evaluate the possibility of using anti-human monoclonal antibody CD3 as pan T-cell marker in the guinea pigs’ trachea and lung in early and late manifestations of the allergic inflammatory process.
Materials and methods.We have studied the distribution and quantitative changes of CD3-positive lymphocytes in trachea and lung of guinea pigs using histological, immunohistochemical, statistical methods in conditions of experimental inflammatory process.
Results. Our results revealed the applicability of anti-Human monoclonal antibody CD3 (Clone SP7, «DAKO», Denmark) cross-reaction with T-cells of guinea pigs’ tracheas and lungs. The most statistically significant elevation of the number of CD3-positive lymphocytes, in comparison with the control group (p*/**<0.05), observed in the experimental group III in the late stages of experimental inflammatory process. The elevation of the number of CD3-positive lymphocytes persists even after the termination of the allergen action, which indicates the continuation of the reaction of pulmonary local adaptive immunity to the allergen.
Conclusions. The results of our study may be useful in conditions of the deficiency of guinea pig-specific tests. The immunohistochemical assessment of guinea pigs’ trachea and lungs proved the possibility to use anti-Human monoclonal antibody CD3 as a panT-cell marker in guinea pigs. We demonstrated the activation of adaptive immune response (T-cells), represented by their immunohistochemical changes, predominantly in the late stages of experimental inflammatory process
Emergency treatment in gynecology using different methods of surgery
Get PDFThe aim. Improving surgical treatment of urgent genital pathology based on comparing the effectiveness of different methods of surgery.
Materials and methods. 170 women were selected and divided into three groups. Group 1 included women with acute pelvic inflammatory disease (aPID); to 2nd group – 60 women with intra-abdominal bleeding (IAB); to 3rd group - 50 women with impaired blood supply to tumors of the internal genitalia (uterus and ovaries) (IBSTIG). Each group was divided into two equivalent subgroups depending on the method of treatment: laparoscopic or laparotomy.
During the study, all examined patients had a medical history, gynecological examination, ultrasound examination, surgical treatment by laparoscopy or laparotomy.
Results. The average duration of laparoscopic operations in patients in subgroup 1.1 was 50.4±7.2 minutes and 80.3±10.1 minutes in subgroup 1.2 – with laparotomy method. Intraoperative blood loss was significantly lower in subgroup 1.1 compared with subgroup 1.2 (210.7±31.4 ml and 375.9±44.5 ml) (p <0.01). In the postoperative period in subgroup 1.1, which included women with emergencies due to aPID and in which operations were performed laparoscopically, the maximum temperature rise after surgery was 37.6±0.04 °C, which was significantly lower than in subgroup 1.2, which included women with emergencies due to aPID and in which operations were performed by laparotomy, this figure was 38.0±0.08 °C (p <0.001). The mean duration of the postoperative period in patients of subgroup 1.1 was significantly shorter compared to subgroup 1.2 (7.2±0.6 and 10.2±1.0 days) (p <0.05).
The average duration of operations in subgroup 2.1 was significantly shorter than in subgroup 2.1 (39.8±2.1 and 59.1±2.5 minutes, respectively) (p <0.001). Intraoperative blood loss was on average significantly lower in subgroup 2.1 (189.1±19.8 and 388.5±37.2 ml, respectively) (p <0.05).
In subgroup 3.1, which included women with IBSTIG and underwent surgical treatment by laparoscopy, the average duration of operations was significantly shorter than in subgroup 3.2, where such women with such pathology received surgical treatment by laparotomy (62.1±4.9 and 89.6±7.8 minutes, respectively) (p <0.01). Blood loss was significantly lower in subgroup 3.1 in contrast to subgroup 3.2 (101.7±10.1 ml and 283.3±12.9 ml, respectively) (p <0.001).
Conclusions. The use of laparoscopy for the treatment of urgent genital pathology could increase the effectiveness of surgical treatment. In addition, this method reduces the duration of surgery and surgical blood loss. As a result, it makes it possible to improve the postoperative period
Evaluation of the sleep hygiene index and physical activity states of the elderly living in nursing homes
Get PDFThe aim: the sleep quality and physical performance play a vital role in older adults’ well-being to maintain good overall health. Therefore, the parameters that have a role in sleep and physical capacity are crucial to achieve a healthy aging outcome. In this study, we aimed at comparing the quality of sleep and physical activity state of the elderly living in 5 nursing homes.
Methods: the 181 elderly individuals living in 5 nursing homes were included in this cross-sectional study. Physical activity, sleep hygiene index and demographics were analysed using SPPS 23.0.
Results: overall, 68.5 % of the participants were male, and 31.5 % were female. There was no significant correlation between the sleep hygiene scores and physical activity states (p >0.05). Those who were divorced, those who were on at least one type of medication, those who smoked and those with poor perception of health had statistically significant difference in terms of the Sleep Hygiene Index (p<0.05).
Conclusion: other than findings of the presented study that influence sleep and physical activity, the many other factors in different geographical areas or cultures could be a reason that is interrelated with sleep quality and physical performance of older adults. In this study, for primary care and family physicians to boost the sleep quality of the elderly, we recommend improving their health perception, quitting smoking, discontinuing unnecessary medications, and increasing their social interaction