ScienceRise: Medical Science
Not a member yet
556 research outputs found
Sort by
Вплив гіполіпідемічних засобів на рівень модуляторів стеатогенезу та фіброгенезу у щурів з неалкогольною жировою хворобою печінки, асоційованою з гіпергомоцистеїнемією
Get PDFThe aim was to determine the influence of Simvastatin and PUFA ω-3 remedy on the level of pro-fibrogenic neurotransmitters (Homocysteine, TNFα) and the level of neurotransmitters that determine anti-fibrous and liver regeneration potential (H2S and IGF-1) under experimental NAFLD associated with HHC.Materials and methods. The study was carried out using 100 white laboratory male rats divided into 7 experimental and 3 control groups. NAFLD associated with HHC was modeled in 7 groups of rats by applying high fat diet with simultaneous administration of Homocysteine thiolactone (100 mg / kg / intragastrically) for 60 days. From the 61st day and till the end of the experiment, 6 groups of rats with NAFLD + HHC had a standard diet. On this background, the animals of 4 groups were treated by lipid-lowering drugs - Simvastatin or ω-3 PUFAs remedy.The levels of Homocysteine, TNF-α and IGF-1 in bloodserum, as well as the content of triglycerides, cholesterol, phospholipids, hydroxyproline and H2S in rat liver were determined.Results. The use of Simvastatin did not cause statistically significant changes in the levels of Homocysteine, TNFα in blood serum and H2S content in the liver, but it led to IGF-1 worsening deficits in blood serum. At the same time, the use of PUFA ω-3 resulted in a significant reduction in the levels of Homocysteine, TNFα and increase in the levels of IGF-1 in serum and H2S in rat liver. Anti-steatosis and anti-fibrous effects of ω-3 PUFA remedy were significantly higher comparing to Simvastatin.Conclusion. Therefore, PUFA ω-3 drug significantly exceeds Simvastatin by hypohomocystinemic effect, more effectively reduces TNFα in blood serum and increases the H2S content in liver, and stimulates an IGF-1 level increase in blood serum, while Simvastatin enhances its deficitДосліджено вплив гіполіпідемічних засобів на рівень модуляторів стеатогенезу та фіброгенезу у щурів з неалкогольною жировою хворобою печінки, асоційованою з гіпергомоцистеїнемією. Показано, що препарат омега-3 поліненасичених жирних кислот ефективніше, порівняно з симвастатином, зменшує рівень профіброгенних медіаторів (гомоцистеїну, TNF-α), коригує дефіцит антифіброзних медіаторів (H2S, IGF-1), зменшує біохімічні ознаки стеатозу та фіброзу печінк
Методи оцінки центральної гемодинаміки при інгаляційній анестезії у пацієнтів з тиреотоксикозом
Get PDFAim: To assess the central hemodynamics using calculation forms and invasive-noninvasive systems on the background of using inhaled minimal-streaming anesthesia at thyroidectomies in patients with thyrotoxicosis.Materials and methods. The study included 44 patients with the syndrome of thyrotoxicosis (diffuse thyrotoxic goiter – 31 patients (70,45 %), multi-node goiter – 10 (22,74 %) and toxic adenoma – 3 patients (6,81 %)), operated under the general anesthesia as inhaled anesthesia by sevoflurane by the method of minimal stream (FGF=400 ml/min., Sev 3,0 vol%). Women – 40 patients (91,9 %), men – 4 (9,1 %) patients. Mean age 47,61±2,39 years, BMI 25,89±0,53 c.u, body surface area 1,82±0,02 m2. The assessment of systolic arterial pressure (АPs), diastolic (APd), mean AR (MAP), sphygmic pressure (SP), HR, mean pressure in the aorta, (MPA). There was studied the index of the proper minute volume of blood circulation (PMCB) based on values of the proper main metabolism (PMM). The parameters of the central hemodynamics were studied intraoperationally (after patient’s intubation) after esophageal dopplerography on the apparatus Cardio Q ("Deltex Medical", Great Britain) and esCCO™ method (monitor Vismo, Nihon Kohden). esCCO™ method (calculative continuous heart ejection) and calculative formulas by: Starr, Lillier-Shtrander, Tsander, RU No. 2481785.Results and discussion. Calculative indices by M.M. Savitsky formula don’t reliably differ comparing with the objective instrumental method of hemodynamics control by DopplerEchoCG and can be used as initial indices for the comparison with instrumental and calculative ones. At the analysis of APs, APd, MAP, SP in control points, it was observed, that at patient’s admission to a surgical room MAP and MPA on 2 control reliably (р<0,001) increased by 7,3 % and 6,7 % respectively comparing with 1 control, connected with the natural anxiety of a patients before the surgical intervention. After the input anesthesia the reliably (р<0,001) lowest indices of MAP, MPA, SP, APs, APs on 3 control by Wilcoxon criterion were observed. They were stabilized at following stages of anesthesia. MVB and HI on all control points, according to the data of calculative formulas by Starr, Lillier-Shtrander and Tsander, have reliably (p<0,05) lower values by Wilcoxon criterion than according to instrumental data by DopplerEchoCG. The calculative indices MVB and HI, according to the RU No.2481785 have reliably (p<0,05) higher values by Wilcoxon criterion on all control points than by DopplerEchoCG instrumental data. MVB, GPVR, HI, obtained using the method of esCCO Vismo monitoring had the least degree of deviations from DopplerEchoCG data. Spearmen correlation between these indices was 0,83 (strong direct connection, p<0,05). Spearmen correlation is absent between indices of the calculative formulas of Starr, Lillier-Shtrander and Tsander and data of DopplerEchoCG (rs=–0,07 and rs=–0,14 respectively). The indices by the data of the patent RU No.2481785 have rather high mistake by the data of DopplerEchoCG comparing with esCCO method, but demonstrate the moderate correlation with them rs=0,38 (p<0,05). There is the strong direct correlation between indices of the invasive CardioQ EDM and non-invasive esCCO method (rs=0,75;p<0,05). Among calculative indices, the most correlation with data of esCCO method is demonstrated by indices by the patent С RU No. 2481785 (rs=0,38, p<0,05), indices by Starr, Lillier-Shtrander and Tsander formulas have no correlation with esCCO method.Conclusions. Calculative indices by M. M. Savitsky formula don’t reliably differ comparing with DopplerEchoCG data and can be used as initial indices for the comparison with instrumental and calculative ones.Calculative indices by the data of Starr, Lillier-Shrander and Tsander formulas underestimate MVB, HI and have no correlations comparing with instrumental assessment methods. Indices by the patent RU No.2481785 give overestimated values of MVB and HI, but have the moderate correlation (rs=0,38; (p<0,05) with both DopplerEchoCG and esCCO data.After the input anesthesia there is observed the reliable (p<0,05) decrease of MVB and HI by doth data of invasive monitoring – esophageal dopplerography by Cardio Q system, and non-invasive esCCO™ monitoring method (calculative continuous hearth ejection), stabilized at 5-6 stages of the operation.There is the moderate correlation between indices of data of invasive (Cardio Q) and non-invasive (esCCO™) monitoring at the level rs=0,75 (p<0,05).esCCO method allows to study fluctuations of the central hemodynamics at equal stages of anesthesia maximally effectively, it is a simple non-invasive method of control of the central hemodynamics with the high degree of correlation with DopplerEchoCg indicesПроведено оцінку центральної гемодинаміки інтраопераційно за допомогою інвазивних/неінвазивних методів та розрахункових формул у пацієнтів з тиреотоксикозом на тлі застосування інгаляційної мінімально-потокової анестезії при виконанні тиреоїдектомій. Розрахункові показники за даними формул Старра, Лілье-Штрандера и Цандера занижують, а за патентом RU №2481785 мають завищені показники хвилинного об’єму кровобігу, серцевого індексу у порівнянні із інструментальними способами оцінк
Дослідження внутрішньоочного тиску при оперативних втручаннях на хребті у положенні на животі
Get PDFAim: to estimate intraocular pressure changes at lumbar spine surgery in prone position at the general intravenous anesthesia and at the spinal anesthesia and to compare these data with healthy volunteers.Materials and methods. The research included 10 healthy volunteers and 40 patients ASA І-ІІ, who underwent planned surgical interventions on the lumbar spine in prone position. Patients of І group (n=20, men 7,women 13, mean age 47±14 years) underwent surgical interventions under conditions of the spinal anesthesia. Patients of ІІ group (n=20, men 8, women 12, mean age 44±12 years) underwent surgical interventions under conditions of the general intravenous anesthesia. Patients’ prone position was horizontal in both groups. The head turned at the angle 45° (the left eye lower than the right one). The intraocular pressure was estimated by Maklakov’s method by one researcher in the position on the spine before surgery and immediately after it. Healthy volunteers (n=10, menв 4, women 6, mean age 49±12 years) were examined in the position on the spine, after that they lied in the analogous prone position during 90 minutes and were examined immediately after turning on the spine.Research results: patients of both groups and healthy volunteers demonstrated the increase of the intraocular pressure after lying in prone position (р<0,001), moreover it was higher in the left eye (the lower one). In patients of 2 group (general anesthesia) IOP increase in the low eye was reliably (р=0,03) more than in patients of I group and healthy volunteers. Patients of I group didn’t demonstrated reliable changes compared with the group of healthy volunteers.Conclusions: at turning in prone position healthy volunteers and patients under anesthesia (spinal, general) demonstrate the intraocular pressure increase. IOP increases in patients under the general anesthesia were reliably higher in the lower eye, than in patients of the group of the spinal anesthesia and healthy volunteersПредставлені результати дослідження внутрішньоочного тиску (ВОТ) у пацієнтів при операціях на хребті у положенні на животі в умовах внутрішньовенної або спінальної анестезії, а також у здорових добровольців. Внаслідок знаходження у положенні на животі ВОТ підвищувався у всіх обстежених, проте більше у нижчерозташованому оці при загальній анестезі
Оптимізація підходів до покращення комплаєнсу хворих до лікування цукрового діабету 2-го типу важкого ступеня тяжкості шляхом залучення сімейної терапії
Get PDFAim. To improve the quality of the treatment of patients with DM type 2 of hard severity degree at the expanse of the increase of medicamentous compliance to patients by family therapy involvement.Materials and methods. There were observed 174 patients with DM type 2 of hard severity degree, who underwent out-patient treatment. They were divided in groups depending on applied tactics of psychoprophylaxis of compliance disorders.Methods of research: clinical-anamnestic; clinical-psychopathologic; psychodiagnostic, statistical. Research results and their discussion. It was established that the one of node dyscompliance factors in patients with DM type 2 of hard severity degree is a disorder of cognitive functions that testify to the necessity of a side help involvement, including patients’ relatives into the control of medical prescriptions realization. The other dyscompliance factor of patients with DM type 2 to the therapy is an attitude of relatives to his/her disease. There were established two types of patients’ reaction to medical recommendations, appeared as a response of patient’s family members of his/her disease. All these reactions had dyscompliant character that conditioned a necessity of the correction work with patient’s relatives as a factor psychoprophylaxis of disorders of compliance to the therapy in patients with DM type 2. The elaborated system of prophylaxis of disorders of medicamentous compliance to the therapy in patients with DM type 2 of hard severity degree consisted of two links: indirect work with patients and work with patients’ family members with family therapy involvement. The high effectiveness of the elaborated the system of prophylaxis of disorders of medicamentous compliance to the therapy in patients with DM type 2 of hard severity degree was proved. In all patients, who underwent psychoprophylaxis by the elaborated system, the compliance level increased to maximal values.Conclusions. There were established the factors of dyscompliance to the medicamentous therapy in patients with DM type 2 of hard severity degree. The influence of family members’ reaction to patient’s disease on features of patient’s attitude to the therapy was determined.The system of prophylaxis of disorders of medicamentous compliance to the therapy in patients with DM type 2 of hard severity degree that included family therapy and direct work with patients’ relatives was elaborated. Its high effectiveness was notedВстановлені фактори дискомплаєнтності до медикаментозної терапії у хворих на ЦД 2-го типу важкого ступеня тяжкості. Визначено вплив реагування членів сім’ї до захворювання хворого на особливості ставлення хворого до терапії.Розроблена система психопрофілактики порушення медикаментозного комплаєнсу до терапії у хворих на ЦД 2-го типу важкого ступеня тяжкості, яка включала сімейну терапію та безпосереднью роботу з родичами хворого. Проаналізовано її ефективніст
Дослідження ультраструктури ендотеліоцитів кровоносних капілярів тимуса щура в нормі та під впливом метилтретбутилового ефіра
Get PDFAim of research is the study of the influence of benzene component, namely methyl tret-butyl ether on the structure of hemomicrocirculatory bed of a thymus. It is known that a thymus is a central organ of the immune system that plays an important role in the formation of the immune response to endo- and exogenous antigens. In the connection of growing environmental pollution, the number of diseases, connected with immune system pathologies essentially increased. Experts think that the increase of the number of immune diseases is a result of soil contamination as an inalienable factor of life activity. Methods of research: terms and conditions of water contamination with methyl tren-butyl ether, approximated to real ones were created for the study. The minimum for contamination is 1/1000 – 1/2000 of the mean toxic dose. Hemomicrocirculatory bed of rat’s thymus and its cells were studied under the influence of different doses (500 mg/kg; 50 mg/kg; 5 mg/kg; 0,5 mg/kg) of methyl tret-butyl ether. White laboratory rats were selected for the study because of well developed thymus and its morphological similarity to a human one.Results: general changes of hemomicrocirculatory vessels are reactive and adaptive ones (І and ІІ stages of pathological process), dystrophic and alterative (ІІІ stage), changes of endotheliocytes and basal membrane in all links of hemomicrocirculatory bed.The manifestation of reactive changes is the change of endotheliocytes configuration, microclasmatos and vesiculation. The adaptation of reactive changes is directed on stabilization of the endotheliocyte order. It is manifested as vesiculation transformation from the transport to membranoplastic type. Conclusions: Morphological study of the vascular ultrastructure of hemomicrocirculatiry bed using electric transmissive microscopy proves that at methyl tret-butyl ether penetration in rat’s organism, its effect results in ultrastructural changes of the microhemocirculatory bed and has a cumulative characterУ статті аналізуються власні дані про структуру гемомікроциркуляторного русла щурів під впливом метилтретбутилового ефиру (500 мг/кг, 50 мг/кг, 5 мг/кг, 0,5 мг/кг) в експерименті. Загальним проявом дії метилтретбутилового ефіра на кровоносні судини тимуса є реактивні, адаптаційні, дистрофічні та альтернаційні зміни ендотеліальних клітин та їх базальної мембрани у ланках гемомікроциркуляторного русл
Патологія ендометрія та репродуктивний профіль жінок в пізньому репродуктивному та пременопаузальному віці
Get PDFThe aim of the study was to determine the features of the structure of intrauterine pathology and obstetric and gynecological history of women in the late reproductive and premenopausal period.Materials and methods. In the observational cross-sectional retrospective study by the continuous sampling method, 849 histories of the disease of women of all age groups with pathology of endometrium were selected. Inclusion criteria: presence of one or more endometrium pathologies - endometrial polyp, endometrial hyperplasia, chronic endometritis, intrauterine sinechia.Results and conclusions. More than a third of patients (37.3%) with pathology of endometrium are women of late reproductive and premenopausal age. Women of late reproductive and premenopausal age are a group of high-risk endometrium pathology, whose structure is dominated by endometrial polyps and endometrial hyperplasia, in combination with endocrine-related pathology. Current is the development of a comprehensive system of diagnosis, prevention and treatment of endometrial pathology in women of late reproductive and premenopausal age on the basis of the etiopathogenetic personified approach.Метою дослідження було визначити особливості структури внутрішньоматкової патології та акушерсько-гінекологічного анамнезу жінок в пізньому репродуктивному та пременопаузальному періоді. Жінки пізнього репродуктивного та пременопаузального віку відносяться до групи високого ризику розвитку патології ендометрія (ПЕ), у структурі якої переважають поліпи та гіперплазія ендометрія в поєднанні з ендокринзалежною патологією. Отже, актуальною є розробка цілісної системи діагностики, профілактики та лікування патології ендометрія на основі етіопатогенетичничного персоніфікованого підход
Спінальна м'язова атрофія – проблеми патогенезу та вибору методів лікування
Get PDFAim. The use of inhibitors of histones deacetylase, especially valproates together with the vitamin therapy and the complex of physical exercises was the main method of the treatment of patients with SMA till today. The elaboration of the first, officially acknowledged FDA preparation for SMA treatment forces to review the expedience of the complex therapy using valporoic acid, vitamin additives and physical exercises. Methods. The study included 12 patients with different forms of amyotrophy. Depending on the complex of received therapeutic arrangements, they were divided in 4 groups. The treatment effectiveness was estimated in points of Hammersmith scale of the motor function. The comparison of the treatment effectiveness in different groups of patients was realized with the calculation of Student coefficients. Result., Motor possibilities of patients in all groups essentially differed from each other depending on SMA type. The number of points, received by patients with the same SMA type, almost didn’t differ depending of a group of patients. Conclusions. Motor possibilities of patients are mainly connected with their initial phenotype, only partially influenced by the used treatment methods. The effectiveness of medical means at SMA depends not on the quantity of selected groups of preparations and additional treatment methods but on the initial phenotypeСпінальна м'язова атрофія (SMA) – генетичне нейродегенеративне захворювання спричинене делецією гену SMN1. Розвиток медичних технологій та генетики дозволили не просто просунутись в розумінні патогенезу даного захворювання, а й розробити перший, визнаний американською організацією FDA, препарат для лікування SMA. Таким чином, доцільність наявних на сьогоднішній день методів лікування аміотрофії, повинна бути переглянут
Методика проведення внутрішньоартеріальної хіміотерапії раку стравоходу
Get PDFEsophagus it is an organ that takes blood from the different sources. Anatomic features of nourishment of esophageal tumors did not allow study them with the help of angiography like the tumors of other organs. In this connection intra-arterial way of chemotherapy drug administration for treating esophageal tumors is practically not studied.Methodology. For creating the maximal concentration of chemotherapy drug in esophageal tumor in the scientific department of the thorax tumors was elaborated the methodology of intra-arterial chemotherapy of esophageal cancer. Patients with cancer of the upper third of esophagus underwent catheterization of the clypeate cervical trunk or lower clypeate artery, middle third – left lower diaphragmatic artery, lower third – left gastric artery. Catheterization of the own esophageal arteries was not realized in any case.Results. At randomized research 34 patients underwent catheterization of esophageal arteries. Locally spread esophageal tumors have the dominant source of the blood supply and several secondary branches. The tumors growth results in hypertrophy of afferent preexisting vessels and development of neovascularization in the injury nidus. Conclusion. The use of intra-arterial chemotherapy in patients with esophageal cancer combined with the radiotherapy can be the method of choice for non-adjunctive and conservative treatmentВнутрішньоартеріальна хіміотерапія раку стравоходу на сьогоднішній день є маловивченою. Мета дослідження: теоретично обґрунтувати та розробити практичні методики артеріальних доступів для селективної хіміотерапії на рак внутрішньогрудного відділу стравоходу. В рамках рандомізованого дослідження у відділенні пухлин органів грудної порожнини Національного інститут раку проведено доопераційну хіміопроменеву терапію 34 пацієнтам. Здійснювали катетеризацію щитошийного стовбура, нижньої щитоподібної артерії, лівої нижньої діафрагмальної артерії або лівої шлункової артерії. Внутрішньоартеріальна хіміотерапія може бути методом вибору для лікування раку стравоход
Стан тромбоцитарного гемостазу у хворих на гіпертонічну хворобу та її поєднання з ішемічною хворобою серця
Get PDFAim of the work is in assessment of spontaneous and induced aggregation in patients with hypertension and revelation of features of aggregative activity of platelets at combination of hypertension with ischemic heart disease.Methods of research: special laboratory studies: spontaneous aggregation of platelets with arachidonic acid, adenosine diphosphate, collagen and adrenalin.Results of research: in the result of the work was revealed the high level of spontaneous aggregation of platelets in both studied groups. It was established, that associated course of hypertension and IHD is attended by preservation of the sufficiently high functional activity of platelets despite the antiplatelet treatment that is typical for patients with hypertension of both II and III stages. Platelets of patients with hypertension of III stage with concomitant IHD even under conditions of aggressive antiplatelet treatment stay more reactive that is manifested in preservation of the high level of spontaneous aggregation and adrenalin-induced aggregation in subgroup of patients who do not receive ß-adrenoblockersВ ході роботи були досліджені пацієнти з гіпертонічною хворобою 2 та 3 стадії і стабільними формами ішемічної хвороби серця та рівень функціональної активності тромбоцитів. В результаті роботи було виявлено збереженням достатньо високої функціональної активності тромбоцитів, незважаючи на проведення антитромбоцитарного лікування, що характерно для хворих на гіпертонічну хворобу як II, так і III стаді
Дослідження якості життя подружжів з різним станом здоровʼя сімʼї та аддиктивної поведінки у жінок
Get PDFAim – the study of life quality of married couples with the different state of family health and addictive behavior (AD) in women for revelation of targets of the further psychocorrectional work with this contingent.Contingent. 321 married couples (MC), who addressed for help because of disorders of family relations and “socially acceptable” addictive behavior in women (studied group, SG). As the groups of comparison there were also examined 50 MC with disorders in family health, where were not the problems of addictive character in women (group of comparison, GC) and 50 conventionally harmonious MC (group of comparison 2, GC 2).Methods. Clinical-psychological and psychodiagnostic ones using the questionnaire of life quality, elaborated by Mezzich, Cohen, Ruiperez, Liu&Yoon (1999).Results. In women with family health disorder the life quality is characterized with essential decrease with heterogenic injury of its separate components, not depending of presence/absence of addictive behavior.The main problem that generates the circle of “pathological” causal connections of the disorder of family functioning in women and formation of addictive behavior in them is the problem of women’s feeling as being psychologically and emotionally “torn away” in family and macrosocial environment. Such situation that is a result of situation-conditioned frustrative (maladjustment) reaction that is, in its turn, conditioned by the presence of emotional and communicative problems between women – family – society, provoke women to solve it in “addictive” way.Conclusions. The basic target of psychological correction and psychoprophylactic support at the family health disorders in women with addictive behavior must be the problem of women’s feeling as being psychologically and emotionally “torn away” in family and macrosocial environment that in most women becomes a background for addictionНа основі аналізу якості життя 421 подружжів,виявлено, що у жінок з порушенням здоровʼясімʼї, незалежно від наявності / відсутності у них аддиктивної поведінки, якість життя характеризується значним зниженням, з неоднорідним ураженням окремих складових. Отримані дані покладені в основу системи психолокорекції та психопрофілактикиздоровʼясімʼї за наявності аддиктивної поведінки у жіно