Zonguldak Bülent Ecevit University Institutional Repository
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Stability of SARS-CoV-2 antibody in serum under various usage and storage conditions
No full textIntroduction: We investigated the effect of two preanalytical variables, temperature change and freezing-thawing of serum samples, on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG levels. Methodology: Serum samples were collected from patients who had coronavirus disease 2019 (COVID-19) prior to vaccination. Six serum samples were included, two each with high positivity (HP), low positivity (LP), and a level of close-to-detection limit (CDL) for SARS-CoV-2 IgG. Each of these six samples was divided into three tubes and placed in refrigerators at 4-8 °C, -20 °C, and -70 °C; removed from the storage temperature once per day for 20 consecutive days; and assayed for SARS-CoV-2 IgG level. Results: The coefficient of variation of all the remaining serum samples were within 95% except for CDL-1 serum at -70 °C, HP-2 serum at 4-8 °C, HP-2 serum at -20 °C, and HP-2 serum at -70 °C. The levels increased significantly when the temperature in the samples with CDL was reduced. The values in samples with LP at -20 °C and -70 °C were significantly higher than those at 4-8°C. In the case of samples with HP, the values of samples at -20 °C were higher than those in samples at 4-8 °C. There was no positive–negative change during any of the freeze-thaw cycles. Conclusions: Antibody value in the samples at 4-8 °C remained stable throughout the 20 freeze-thaw cycles. The antibody value of the samples at -20 °C and -70 °C tended to elevate.</jats:p
Comparison of screw fixation and dynamic fixation in the treatment of ankle fractures with syndesmotic ruptures
No full textSyndesmosis injuries in ankle fractures can significantly impact patient mobility and recovery, making the choice of fixation method crucial for optimal outcomes. This study aimed to compare the quality of reduction and functional results between screw fixation and dynamic fixation in treating syndesmosis injuries in ankle fractures.This cohort study included 48 patients (28 males, 20 females) with an ankle fracture accompanied by syndesmosis injury. Twenty-four patients were treated with single-level TightRope fixation, while another 24 patients received single 3.5-mm cortical screw fixation. The clinical outcomes were measured using the American Orthopaedic Foot & Ankle Society (AOFAS) score, the Olerud-Molander Ankle Score (OMAS), the Visual Analog Scale (VAS) score, and the Foot and Ankle Disability Index (FADI).The mean age of the patients was 37.3±15.1 years. The mean follow-up period was 27.6±13.5 months. There were no significant differences between the groups treated with syndesmotic screw or TightRope in terms of the mean postoperative one-year AOFAS score (89.0 and 86.0, respectively), OMAS (84.5 and 85.1, respectively), and FADI (85.4 and 86.8, respectively). The difference between preoperative and postoperative VAS scores was statistically significant (p=0.020). At the first-year follow-up, the median medial clear space was 4.3 mm (range: 2.1 to 5.7 mm) and 4.3 mm (range: 2.3 to 5.7 mm) in the two groups, respectively. The median tibiofibular clear space was 4.8 mm (range: 3.4 to 6.4 mm) in the screw fixation group and 5.1 mm (range: 4.0 to 6.8 mm) in the dynamic fixation group. Meanwhile, the median tibiofibular overlap was 7.8 mm (range: 4.2 to 10.4 mm) and 7.9 mm (range: 4.4 to 10.9 mm) for the screw fixation and dynamic fixation groups, respectively, one year post-surgery.The dynamic fixation method is as functional as the screw fixation method. Early full weight-bearing and improved pain control were noted as advantages of dynamic fixation compared to screw fixation
Liquid–liquid microextraction method based on switchable hydrophilic solvent for determination of parabens in cream samples
No full textThe Mediation Role of Coping With Stress in the Relationship Between Psychological Flexibility and Posttraumatic Growth in Cancer Patients
No full text Objectives: Active and adaptive coping strategies have been identified as factors that can assist cancer patients in effectively managing and overcoming the crisis, leading to the potential for post-traumatic growth during the diagnosis and treatment stages. Psychological flexibility is another factor that can protect individuals and promote more adaptive reactions to traumatic situations. The study aims to examine the investigation of the mediation role of coping with stress in the relationship between psychological flexibility and posttraumatic growth in cancer patients. Methods: This study was conducted with 97 (63 women, 34 male) participants aged between 38 and 82 who are literate and diagnosed with breast cancer or gynecological cancers (endometrial, cervical, vulvar) undergoing radiotherapy planning at the Radiation Oncology Clinic of Zonguldak Bülent Ecevit University Hospital. Demographic Information Form, Psychological Flexibility Scale, Post-Traumatic Growth Inventory, and Coping Styles Scale were applied to the participants. The mediation effects were interpreted using bootstrap confidence intervals and VAF values. Results: This study showed that the active coping styles partially mediated the relationship between psychological flexibility and posttraumatic growth. Conclusion: In this regard, by organizing psychoeducational programs and interventions aimed at increasing psychological flexibility for individuals diagnosed with cancer or undergoing treatment, individuals can be enabled to cope with stress more actively, and thus post-traumatic growth can be achieved. </jats:p
Hastalara Verilen Tıbbi Atıkların Kontrolü ve Sıfır Atık Yönetmeliği Eğitiminin Bilgi Düzeylerine Etkisi
No full textGiriş: Sağlık kurumlarında atıkların yönetimi, atık çeşitliliğinden dolayı birden fazla yönetmelik kapsamında yürütülebilmekte, bu durum da atık yönetimini zorlaştırabilmektedir. Amaç: Çalışma hastalara verilen tıbbi atıkların kontrolü ve sıfır atık yönetmeliği eğitiminin bilgi düzeylerine etkisini değerlendirme amacıyla yapıldı. Yöntem: Ön test-son test tek gruplu yarı deneysel araştırma türünde yapılan bu çalışmaya basit rastgele örnekleme yöntemiyle, örneklem kriterlerine uyan 70 hasta dahil edildi. Veriler Sosyodemografik Bilgi Formu ve Tıbbi Atıkların Kontrolü ve Sıfır Atık Yönetmeliği Bilgi Testi ile toplandı. Tıbbi Atıkların Kontrolü ve Sıfır Atık Yönetmeliği eğitimi araştırmacılar tarafından QR karekodlu broşürle verildi. Verilerin analizinde, yüzdelik, ortalama, standart sapma, bağımsız gruplarda t test, bağımlı gruplarda t test, tek yönlü ANOVA, games howell testleri kullanıldı. Bulgular: Çalışmaya katılan bireylerin %41,4’ü 38 - 57 yaş arasında, %68,6’sı kadın, %42,9’u ilköğretim mezunudur. Bilgi puan ortalamalarının girişim öncesinde 13,01 ± 3,78, girişim sonrasında 15,20 ± 3,30 bulundu (p &lt; 0,001). Girişim sonrasında yükseköğretim mezunu bireylerin bilgi puanı ortalamalarının (17,73 ± 1,96), ortaöğretim (15,38 ± 2,81) ve ilkokul mezunlarından (13,46 ± 3,28) daha yüksek olduğu saptandı (p &lt; 0,001). Yaş aralığı 18 - 37 yıl olan bireylerin girişim öncesi de girişim sonrası da bilgi puan ortalamalarının daha yüksek olduğu saptandı (p &lt; 0,05). Sonuç: Tıbbi atıkların kontrolü ve sıfır atık yönetmeliği eğitiminin hastaların bilgi düzeylerini artırdığı, yaşı genç ve eğitim seviyesi yüksek olanların bilgi düzeylerinin daha yüksek olduğu saptandı.</jats:p
Determination of trace amounts of metobromuron herbicide residues in fruits by QuEChERS and DLLME methods
No full textFood products have the potential to contain herbicide residues that may pose a threat to human health and the environment, and the determination of these residues at trace levels is extremely important. In this study, a rapid, simple, accurate and environmentally friendly DLLME-QuEChERS method was developed for the sensitive determination of metobromuron at trace levels in coconut-strawberry samples. Mass spectrometry coupled to gas chromatography was used to perform the analysis. Limit of detection (LOD) and limit of quantitation (LOQ) for the DLLME-GC-MS method providing a highly linear range with a coefficient of determination of >0.99, were found to be 4.2 and 13.9 ng/mL, respectively. Following QuEChERS and DLLME, recovery studies on coconut-strawberry samples showed that the developed method can be accurately applied to food matrices with the giving results close to 100 % and low standard deviation values
TSPO’nun (18 kDa Translokatör Protein) Yapısı, İşlevi ve Patolojik Süreçlerdeki Rolü
No full textFarmakolojik ve yapısal olarak santral benzodiazepin reseptörlerinden farklı olan TSPO, ilk olarak böbrekte diazepam için bağlanma alanı olarak keşfedilmiş ve beyin dışı yerleşimi nedeniyle “periferik benzodiazepin reseptörü” olarak tanımlanmıştır. Daha sonra periferik dokularla birlikte merkezi sinir sistemindeki glial hücreler, endotelyal hücreler ve bazı nöron popülasyonlarında da yüksek oranda bulunduğu tespit edildiğinden bu protein, “18 kDa Translokatör Protein (18 kDa TSPO)’’ olarak yeniden adlandırılmıştır. Mitokondri dış zarında yerleşik olan TSPO, hücre içindeki konumu itibariyle kolestrolün mitokondriye taşınmasını sağlayarak steroid sentezi, proliferasyon, inflamasyon ve apopitoz gibi birçok önemli süreçte rol oynamaktadır. TSPO’nun farklı patolojilerdeki etkilerinin araştırılması amacıyla Ro5-4864 ve PK11195 başta olmak üzere çeşitli TSPO ligandları kullanılmaktadır. TSPO ve ligandları; Alzheimer hastalığı (AH), frontotemporal demans, multipl skleroz, Huntington hastalığı, amyotrofik lateral skleroz ve Parkinson hastalığı gibi nörodejeneratif hastalıkların tanı ve tedavisinde önemli farmakolojik hedefler haline gelmiştir. Ayrıca hücre çoğalmasında öncül proteinlerin mitokondriye taşınmasında da görevli olduğundan bazı malignitelerdeki etkileri de incelenmektedir. Çalışmamızda TSPO’nun çeşitli fizyopatolojik süreçlerdeki rolleri ele alınarak potansiyel tanı ve tedavi hedefi olarak değerlendirilmesi amaçlanmıştır.</jats:p
Effect of Long Glass Fiber Orientations or a Short-Fiber-Reinforced Composite on the Fracture Resistance of Endodontically Treated Premolars
No full textThis study aimed to evaluate the effect of direct restorations using unidirectional glass fiber orientations and a short-fiber-reinforced composite (SFRC) on the fracture resistance of endodontically treated premolars with mesio-occluso-distal cavities. Ninety double-rooted premolars were selected. Fifteen teeth were left intact/as a control group. The endodontic treatment and cavity preparations of seventy-five teeth were performed and divided into five experimental groups: Resin composite (RC), modified transfixed technique + RC, circumferential technique + RC, cavity floor technique + RC, and SFRC + RC. All teeth were fractured under oblique static loading at a 30° angle using a universal testing machine. The fracture patterns were observed and classified. Data were analyzed with one-way analysis of variance, Pearson chi-square, and Tukey HSD post hoc tests (p = 0.05). The highest fracture strength values were obtained in intact teeth (599.336 N), followed by modified transfixed + RC treated teeth (496.58 N), SFRC + RC treated teeth (469.62 N), RC (443.51 N), circumferential + RC treated teeth (442.835 N), and cavity floor + RC treated teeth (404.623 N) (p < 0.05). There was no significant difference between the RC and the circumferential technique + RC (p > 0.05). Unrepairable fractures were observed at low rates (20%) in the modified transfixed + RC and SFRC + RC teeth, and at higher rates in RC (73.3%), cavity floor + RC (60%), and circumferential + RC (80%) teeth. The application of an SFRC or the modified transfixed technique yielded an improved fracture strength and the fracture pattern of ETPs being restored with a universal injectable composite.</jats:p
Retrospective Investigation of Alcohol Intoxications Followed in Intensive Care Unit
No full textAim: The majority of alcohol-related deaths are due to acute alcohol consumption. There are many factors affecting the prognosis of alcohol toxicity. It has been reported that by determining these factors, mortality rates can be reduced by early diagnosis and early initiation of treatment. In this study, we aimed to determine the prognosis by evaluating the clinical status and laboratory factors of patients followed up in intensive care unit (ICU) due to acute alcohol intoxication. Materials and Methods: The study included 21 patients with acute alcohol intoxication who were followed up in the ICU of our hospital between 2013-2021.Laboratory parameters, demographic characteristics and clinical status of the patients were recorded. Patients were divided into both exitus and survivors and according to the type of alcohol consumed (ethanol and methanol). Results: All patients were male with a mean age of 40.10 ± 15.9 years. 52.4% of the admissions were ethanol poisoning and 47.6% were methanol poisoning. Mortality rate was 33.3%. It was observed that symptoms appeared later in methanol intoxication compared to ethanol intoxication (p</jats:p
LGBTIQ+ Employees’ Coping Strategies to Deal with Gender Discrimination at Work: A Qualitative Study
No full textThe objective of this study is to elucidate the coping mechanisms that LGBTIQ+ individuals employ in response to discrimination based on their sexual orientation and gender identity in the context of business life in Turkiye, with a particular focus on the influence of prevailing social norms and legal deficiencies. It is possible that LGBTIQ+ individuals may refrain from pursuing their rights in the event of discrimination. Consequently, LGBTIQ+ employees develop individual coping strategies in response to discriminatory practices. In this study, interviews were conducted with 19 LGBTIQ+ employees. The objective of the interviews is to ascertain the strategies that employees utilize to cope with discrimination. The structure and generally accepted norms of Turkish society, which justify LGBTIQ+ individuals’ concerns about discrimination and exclusion from social life, have formed the basis of scientific curiosity for this research project. The data obtained from the interviews were transcribed and subjected to a descriptive analysis. The findings were subsequently classified into the following categories: hiding in the workplace, hiding as a result of witnessing discrimination, living a facade, remaining silent in the face of discrimination, confronting discrimination, and not seeking legal rights. The findings indicate that the most prevalent coping strategy employed by LGBTIQ+ individuals is the concealment of their sexual orientation and gender identity. This approach often results in a sense of disconnection from their authentic selves in professional settings. Moreover, the primary strategies employed by LGBTIQ+ employees are identified as working for organizations that are relatively accommodating LGBTIQ+ individuals, working with acquaintances, and imitating a heterosexual lifestyle. The data indicate that individual coping strategies have been employed more frequently by gay and bisexual individuals than by transgender individuals.</jats:p