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De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab
Get PDFRed king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) are deep-sea crustaceans widely distributed in the North Pacific and Northwest Atlantic Oceans. These giant predators have invaded the Barents Sea over the past decades, and climate-driven temperature changes may influence their distribution and abundance in the sub-Arctic region. Molting and growth in crustaceans are strongly affected by temperature, but the underlying molecular mechanisms are little known, particularly in cold-water species. Here, we describe multiple regulatory factors in the two high-latitude crabs by developing de novo transcriptomes from the molting gland (Y-organ or YO) and eye stalk ganglia (ESG), in addition to the hepatopancreas and claw muscle of red king crab. The Halloween genes encoding the ecdysteroidogenic enzymes were expressed in YO, and the ESG contained multiple neuropeptides, including molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), and ion-transport peptide (ITP). Both crabs expressed a diversity of growth-related factors, such as mTOR, AKT, Rheb and AMPKα, and stress-responsive factors, including multiple heat shock proteins (HSPs). Temperature effects on the expression of key regulatory genes were quantified by qPCR in adult red king crab males kept at 4 °C or 10 °C for two weeks during intermolt. The Halloween genes tended to be upregulated in YO at high temperature, while the ecdysteroid receptor and several growth regulators showed tissue-specific responses to elevated temperature. Constitutive and heat-inducible HSPs were expressed in an inverse temperature-dependent manner, suggesting that adult red king crabs can acclimate to increased water temperatures.publishedVersio
Impact of High-Pressure Processing (HPP) on Selected Quality and Nutritional Parameters of Cauliflower (Brassica oleracea var. Botrytis)
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Potential antihypertensive effect of protein by-products from whitefish filleting after simulated gastrointestinal digestion
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An assessment of voltammetry on disposable screen printed electrodes to predict wine chemical composition and oxygen consumption rates
Get PDFThe present work aimed at determining the applicability of linear sweep voltammetry coupled to disposable carbon paste electrodes to predict chemical composition and wine oxygen consumption rates (OCR) by PLS-modeling of the voltammetric signal. Voltammetric signals were acquired in a set of 16 red commercial wines. Samples were extensively characterized including SO2, antioxidant indexes, metals and polyphenols measured by HPLC. Wine OCRs were calculated by measuring oxygen consumption under controlled oxidation conditions. PLS-Regression models were calculated to predict chemical variables and wine OCRs from first order difference voltammogram curves. A significant number of fully validated models predicting chemical variables from voltammetric signals were obtained. Interestingly, monomeric and polymerized anthocyanins can be differently predicted from the first and second wave of the first derivative of voltammograms, respectively. This fast, cheap and easy-to-use approach presents an important potential to be used in wineries for rapid wine chemical characterization.An assessment of voltammetry on disposable screen printed electrodes to predict wine chemical composition and oxygen consumption ratessubmittedVersio
Optimum contribution selection (OCS) analyses prompted successful conservation actions for Faroese horse population
Get PDFThe Faroese horse, an endangered indigenous horse breed, is a part of the cultural and societal heritage of the Faroe Islands. Population history describes a severe bottleneck, prompting for quantification of the genetic diversity (level of inbreeding, probability of gene origin, effective population size) and assessment of sustainable conservation potential (Optimum Contribution Selection, OCS) of the Faroese horse population. The pedigree completeness (PCI) of the Faroese horse is adequate for a realistic estimation of the level of inbreeding (PCI5 = 0.96). In concordance with the known population history, the average inbreeding is exceptionally high; in the last cohort, it was equal to 26.8%. An estimate of the effective population size, based on individual increase in inbreeding and coancestry, accounting for the whole population history, was eight. OCS offers a tool to understand and control the increase in the average relationships in the population. Within a fixed number of matings, the repetitive use of stallions resulted in the lowest level of average relationships. Successful follow-up of mating schemes planned together with a holistic assessment of the suitability of an individual as a breeding candidate, will minimize the increase in inbreeding in future generations and maximize the possibility to increase the census size of the Faroese horse population.Optimum contribution selection (OCS) analyses prompted successful conservation actions for Faroese horse populationpublishedVersio
CURRENT AND FUTURE GENETIC TECHNOLOGIES FOR FISHERIES AND AQUACULTURE: IMPLICATIONS FOR THE WORK OF FAO
Get PDFWithin the last few decades, advances in genetic technologies have created powerful and efficient tools for fisheries stock identification, genetic improvement and domestication of aquaculture species and characterization of changes in aquatic life due to environmental or anthropogenic influences. Emerging genetic tools are improving our understanding of organisms in aquatic ecosystems, in terms of diversity, distribution, abundance, movement, function and adaptation, and are being applied in aquaculture facilities and across fishery and aquaculture value chains. In this study expert elicitation was used to examine and predict current and potential future (10 year) impacts of the application of these novel technologies in fisheries and aquaculture. Highlighting the need to prepare sectors for likely changes that will follow. All suggestions received were collated into themes, to provide a conceptual framework that partitions potential impacts, and calls for required action — action required on governance, management and practical application of these innovations at both a national and international level.CURRENT AND FUTURE GENETIC TECHNOLOGIES FOR FISHERIES AND AQUACULTURE: IMPLICATIONS FOR THE WORK OF FAOpublishedVersio
Determination of the Oxidative Stress Biomarkers of 8-Hydroxydeoxyguanosine and Dityrosine in the Gills, Skin, Dorsal Fin, and Liver Tissue of Atlantic Salmon (Salmo salar) Parr
Get PDFOxidative stress is a condition caused by an imbalance in the occurrence of reactive oxygen species in the cells and tissues of organisms. An ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC–ESI–MS/MS) method was developed for the simultaneous determination of two oxidative stress biomarkers, 8-hydroxydeoxyguanosine (8OHDG) and dityrosine (DIY), in the gills, skin, dorsal fin, and liver tissue of Atlantic salmon (Salmo salar) parr. The use of target analyte-specific 13C and 15N internal standards allowed quantification of each target analyte to be performed through the standard solvent calibration curve. The relative recoveries [mean ± (relative standard deviation%)] of 8OHDG and DIY were 101 ± 11 and 104 ± 13% at a fortified concentration of 10 ng/mL (8OHDG) and 500 ng/mL (DIY), respectively, ensuring the accuracy of the extraction and quantification. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. The limits of detection (LODs) were 0.11 and 1.37 ng/g wet weight (w.w.) for 8OHDG and DIY, respectively. To demonstrate the applicability of the developed method, it was applied in 907 tissue samples that were collected from Atlantic salmon parr individuals reared in an experimental land-based recirculating aquaculture system (RAS) treated with peracetic acid. Moreover, the possibility of using the dorsal fin as an alternative matrix for the minimally invasive assessment of oxidative stress in Atlantic salmon parr was introduced. To our knowledge, 8OHDG and DIY were used for the first time as biomarkers for biomonitoring the fish health (oxidative stress) of Atlantic salmon parr in RAS.Determination of the Oxidative Stress Biomarkers of 8-Hydroxydeoxyguanosine and Dityrosine in the Gills, Skin, Dorsal Fin, and Liver Tissue of Atlantic Salmon (Salmo salar) ParrpublishedVersio
Discolouration of Potato Tubers Under Retail Light: Cultivar Variations and Effect of Different Packaging Materials for Folva Potatoes Stored at 20 and 6 °C
Get PDFLight exposure of potatoes induces formation of both chlorophyll (greening) and of toxic glycoalkaloids (GAs). Greening leads to rejection by consumers and thus to food waste and economic loss. The aim of this study was to (1) study light sensitivity with respect to colour changes and GA development for different Norwegian grown potato cultivars stored at 20 °C and (2) evaluate the light protective effect of selected packaging materials on colour development in cv. Folva at 6 °C and 20 °C. Potatoes of seven cultivars were stored under LED illumination for 4 days at 20 °C. Changes in colour were measured during storage by Minolta Chroma meter and by visually assessing the limit for unacceptable change of colour. The tested cultivars became unacceptable at different times (24–60 h) and differed both in absolute colour values and relative changes of values. The levels of total glycoalkaloids in cultivars with and without light exposure did not correspond well to the changes in colour. Potatoes of cultivar Folva were packaged in materials with different light barrier properties, followed by LED illumination for 4 days at 20 °C and 18 days at 6 °C. None of the tested packaging types provided sufficient protection from light. All potatoes at 20 °C were unacceptably green after 2 days in light (16 h/day) while the potatoes at 6 °C were unacceptably green after 9 days. Packaging material for potatoes cv. Folva should aim for a total light transmittance below 0.02 W/m2 to avoid development of green colour during light exposure at 20 °C in grocery stores.Discolouration of Potato Tubers Under Retail Light: Cultivar Variations and Effect of Different Packaging Materials for Folva Potatoes Stored at 20 and 6 °CpublishedVersio
CO2 packaging increases shelf life through reduction of off-odor production by CO2 tolerant bacteria in addition to growth inhibition of the spoilage bacteriota
Get PDFOptimized packaging conditions to improve the shelf life of chicken fillets is important to prevent food spoilage and food waste. Anaerobic packaging with CO2 or by vacuum packaging is commonly used to increase the shelf life of skinless chicken fillets, but the literature is inconsistent about the spoilage bacteriota. The aim of this work was to determine which bacterial genera that spoil raw chicken fillets packaged under two common packaging conditions and how the packaging gas itself affects the production of off odors for these genera. The spoilage potential of Pseudomonas, Carnobacterium, Hafnia, Serratia, Brochothrix and Shewanella isolated from spoiled chicken fillets was evaluated. Fresh chicken fillets were inoculated with mono- and multi genera strain cocktails (4 log CFU/cm2) and packaged with 100% N2 or 60% CO2/40% N2, stored at 4 °C, and bacterial numbers, bacteriota, gas in headspace and sensory profiles assessed. Additionally, the effect of CO2 on the production of off-odors from fillets inoculated with similar levels of Shewanella spp. or Brochothrix spp. was determined by both sensory profiling and measuring volatile organic components. All bacterial cocktails grew relatively well in chicken meat packed with 100% N2, while 60% CO2/40% N2 resulted in growth inhibition of all isolates compared to 100% N2. All genera except Serratia and Pseudomonas gave rise to off-odors after 11 days of storage in 100% N2. During storage in 60% CO2/40% N2, only fillets with Carnobacterium spp. and Brochothrix spp. showed significantly higher intensities of off-odors compared to the reference fillets. Shewanella spp. and Brochothrix spp. also exhibited significantly higher intensities of negative odor attributes during storage in 100% N2 compared to 60% CO2/40% N2, at a similar total bacterial count. Thus, CO2 improves shelf life not only by reduction of the growth of CO2 tolerant and sensitive bacteria, but also through inhibition of the production of off-odors.CO2 packaging increases shelf life through reduction of off-odor production by CO2 tolerant bacteria in addition to growth inhibition of the spoilage bacteriotapublishedVersio
A Three-Day Intervention With Granola Containing Cereal Beta-Glucan Improves Glycemic Response and Changes the Gut Microbiota in Healthy Individuals: A Crossover Study
Get PDFIntake of soluble fibers including beta-glucan, is known to improve post-prandial glycemic response. The mechanisms have been attributed to the viscous gel forming in the stomach and small intestine, giving a longer absorption time. However, recent evidence suggests a link between intake of beta-glucan and improved glycemic regulation at subsequent meals through the gut microbiota. We investigated the short-term effect of granola with different amounts of cereal beta-glucan on glycemic response and gut microbiota. After a two-week run-in period (baseline), fourteen healthy, normal weight adults completed a dose-response dietary crossover study. Different amounts of cereal beta-glucan (low: 0.8 g, medium: 3.2 g and high: 6.6 g) were provided in granola and eaten with 200 ml low-fat milk as an evening meal for three consecutive days. Blood glucose and insulin were measured fasted and after an oral glucose tolerance test (OGTT) the following day, in addition to peptide YY (PYY) and glucagon-like peptide (GLP-2), fasting short chain fatty acids (SCFA) in blood, breath H2, and gut microbiota in feces. Only the intervention with medium amounts of beta-glucan decreased blood glucose and insulin during OGTT compared to baseline. Fasting PYY increased with both medium and high beta-glucan meal compared to the low beta-glucan meal. The microbiota and SCFAs changed after all three interventions compared to baseline, where acetate and butyrate increased, while propionate was unchanged. Highest positive effect size after intake of beta-glucan was found with Haemophilus, followed by Veillonella and Sutterella. Furthermore, we found several correlations between different bacterial taxa and markers of glycemic response. In summary, intake of granola containing 3.2 g cereal beta-glucan as an evening meal for three consecutive days reduced the glycemic response after an OGTT 0-180 min and changed gut microbiota composition. Since we cannot rule out that other fiber types have contributed to the effect, more studies are needed to further explore the effect of cereal beta-glucan on glycemic regulation.A Three-Day Intervention With Granola Containing Cereal Beta-Glucan Improves Glycemic Response and Changes the Gut Microbiota in Healthy Individuals: A Crossover StudypublishedVersio