Journal of Experimental and Molecular Biology
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Collateral Prospects of Polyherbal Formulation as Antidiabetic and Appraising its Toxicity in Early Development of Zebrafish (Danio Rerio) Larvae: Antidiabetic prospects of FASAME
No full textThe polyherbal formulation was prepared using leaves, flowers, fruits of these specific plants Ficus religiosa, Allium sativum, Senna auriculata, Andrographis paniculata, Momordica charantia, Eugenia jambolana (FASAME). The hydroalcoholic extracts of the FASAME and its phytochemical constituents was determined. For toxicity study, the zebra fishes are used as the experimental models and were exposed to different concentration of FASAME formulation (100 μg, 70 μg, 50 μg, 10 μg, and 5 μg). Then its viability and its lethal effect was calculated. Finally the antidiabetic activity of FASAME was determined by β - galactosidase assay.The phytochemical constituents like flavonoids, steroids, terpenoid, saponins, resins, carbohydrates, proteins and essential oil was present in the hydroalcoholic extract of FASAME polyherbal formulation .The effect of FASAME formulation on Zebra fish larvae has a substantial increase in the percentage of viability at their lower concentration. The FASAME formulation exhibited selective anti-diabetic activity. These results showed that the FASAME formulation has anti-diabetic activity and can be used for the treatment of diabetics.The phytochemical analysis of FASAME drug has a mixture of phytochemical compounds and their IC50 value was found to be 74.39 μg/ml. The toxicity analysis on Zebra fish larvae also showed higher viability at their lower concentration and proved to be a powerful antidiabetic drug for diabetics
The academic Gabriel Corneanu (1942 - 2019) - The man who placed profession above it all
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ESTIMATION OF THE EFFECTS OF CHITOSAN-IRON NANOCOMPOSITES DEVELOPED BY DIFFERENT PROCESSES ON RH. GRACILIS CNMN-Y-30 YEAST
Get PDFAbstract. The paper provides information on the estimation of the effects of iron chitosan nanocomposites, elaborated by different procedures on pigmented yeast Rh. gracilis CNMN-Y-30. It was found that the initial amount of chitosan, the concentration of Fe3O4 nanoparticles and the volume of nanocomposite used for growing yeasts are the main factors that influence the efficiency of chitosan-iron nanocomposites. Microbiological indices adequately reflect the effects of chitosan-iron nanocomposites in the process of evaluating the action of nanocomposites obtained by different processes on the representative yeast Rh. gracilis CNMN-Y-30 and it is recommended to test the degree of influence of the nanocomposite. This information can be used by specialists in the food industry, microbiology, medicine, cosmetology, environmental protection, etc., where nanocomposites have applications
Academician PETRE JITARIU (1905-1989) – man and founder of the Romanian school of biomagnetism
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NANOPARTICLES: AN OVERVIEW ABOUT THEIR CLASIFICATIONS, SYNTHESIS, PROPERTIES, CHARACTERIZATION AND APPLICATIONS
Get PDFNanoparticles (NPs) are the main product of nanotechnologies. NPs are organic and inorganic structures, their size being less than one hundred nanometers. Due to their potential application in many fields metallic NPs are becoming increasingly important. There are numerous organisms possessing the ability to synthesise NPs and which therefore have the potential to be exploited and modified to optimise them to fulfil this purpose. Therefore, many bacteria, fungi and plants have shown the ability to synthesise metallic NPs but all have their own advantages and disadvantages. This “green” method of biological NPs production is a promising approach that allows synthesis in aqueous conditions, with low energy requirements and low-costs. The development of an environmentally friendly and inexpensive way of synthesising them is therefore crucial.
 
SIDE COMPARATION OF TWO METHODS FOR QUANTIFYING MALONDIALDEHYDE LEVELS IN ANIMAL TISSUE EXTRACTS
Get PDFMalondialdehyde (MDA) as an important marker used for the assessment of the oxidative level in a tissue of biological fluid. The standard assay method uses thiobarbituric acid for spectrophotometric detection but suffers from a lack of specificity. Here we show that an HPLC based method for quantitating the MDA has the advantages of increased sensitivity as well as a better specificity, allowing the detection of lower MDA levels in tissue extracts with increased accuracy
INDIRECT ORGANOGENESIS OF SYMPHYTUM OFFICINALE L.
Get PDFymphytum officinale L. (Boraginaceae) is a well-known medicinal plant and a source of natural compounds with high antioxidant activity. The initiation of “in vitro” cultures of Symphytum officinale L aimed not only to assess the dedifferentiation capacity depending on explant origin and growth regulators, but also to develop a multiplication protocol based on indirect regeneration through shoots, followed by roots development induction. The proliferative capacity was tested on leaf and shoots explants, cultivated on Murashige-Skoog basal medium ( MS ), testing two auxins:: naphtalenacetic acid (NAA) and indolylacetic acid (IAA) and two cytokinines: kinetine (K) and benzylaminopurine (BAP). The MS medium with 1.0 mg/l IAA and 0,1 mg/l BAP proved to be the best for callus induction from leaf explants. Shoot regeneration was achieved after subculturing the calli on MS medium supplemented with 1 mg/l BAP and 0,1 mg/l IAA. It was found to be the best for multiple shoot regeneration from callus through organogenesis Multiple shoot proliferation was noticed at 3th subculture in medium and shoot proliferation was decreased with the increased number of subculture. Root system development was achieved on MS medium without growth regulators. Rooted shoots (plantlets) were gradually acclimatized
THE EFFECT OF RHIZOPHAGUS IRREGULARIS AND MESORHIZOBIUM LOTI CO-INOCULATION ON LOTUS JAPONICUS
No full textPlants establish symbiotic relationships with soil bacteria or fungi, which colonize the plant root and provide the plant with inorganic nutrients, in exchange for photosynthesis products. Legume plants associate with both arbuscular mycorrhizal fungi (AMF) and the nitrogen-fixing soil bacteria called rhizobia. During the legume-rhizobium symbiosis, biological nitrogen fixation takes place in specific plants organs formed on the root, called nodules. Using the model legume Lotus japonicus, we studied the establishment of the legume-rhizobia-AMF tripartite symbiosis. We examined how the AM fungus Rhizophagus irregularis and the rhizobium Mesorhizobium loti affected one another during the colonization of the same legume roots, by performing co-inoculations. Moreover, we monitored the effect of the co-inoculation on the general plant performance. According to our results, the presence of M. loti had no effect on the root colonization by R. irregularis. However, root colonization by R. irregularis had a positive effect on the formation of root nodules. This study aims to enhance our understanding on how the plant selects, combines and controls its symbionts, towards to a more efficient use of legume plants in agroecosystems
SALINITY AND SELENIUM NANOPARTICLES EFFECT ON ANTIOXIDANT SYSTEM AND MALONDIALDEHYDE CONTENT IN OCIMUM BASILICUM L. SEEDLINGS
Get PDFSelenium (Se) is an essential nutritional element and its presence has a crucial role in fortification of crops. The aim of the work was to investigate the possible use of selenium nanoparticles (SeNPs) in the mitigation of salinity stress on basil (Ocimum basilicum L.) seedlings. Non-enzymatic compounds, enzymatic activity and malondialdehyde (MDA) as indicator of lipid peroxidation products were quantified under the influence of SeNPs and two concentrations of NaCl (50 mM and 100 mM) applied singular or combined. The activity of antioxidant enzymes in O. basilicum seedlings was generally lower in the control regardless of the treatment applied singular or combined. The same decreasing trend was noted in the case of total polyphenols and MDA content. On the other hand, both combined treatments stimulated the soluble protein content in relation to the single variant, the increase being more pronounced at 50mM + SeNPs in respect with the singular treatment
PINUS NIGRA ARN. AND THE INFRATAXON PINUS NIGRA SSP. BANATICA A SHORT REVIEW
Get PDFBanat black pine is an infrataxon sometimes considered as subspecies, sometimes as variety of black pine, with natural spreading area in southwestern Romania, on Banat Mountains, from where it slightly extends into south of the Danube, in Serbia. The controversy surrounding the position of Banat black pine in southwestern Romania has been known since the last century. The Banat black pine population from this part of Romania has never been thoroughly studied at the molecular level. A future direction of study could be that variability analysis and genetic structure of individuals from natural population of Pinus nigra ssp. Banatica in southwestern Romania, using genetic markers