Journal of Experimental and Molecular Biology
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Abstracts book of the Anual International Conference of the Romanian Society for Biochemistry and Molecular Biology
No full textThe issues containes all the abstracts presented as lectures and posters at the Anual International Conference of the Romanian Society for Biochemistry and Molecular Biology, 26-27th September, Iasi, Romani
GENETIC VARIABILITY OF PHARMACOKINETICS AND PHARMACODYNAMICS OF ANALGESICS (LAYERED MEDICINE)
Get PDFPain therapy, the most widely spread disorder, tends more as other diseases, to administration of drug molecules targeted to the affected tissue at the right dose, or to the patient or patient groups (personalized medicine). A decisive determinant of this strategy is the genetic one, which is to some extent the basis of the variability of pharmacokinetic and pharmacodynamic response to analgesics in the patient population. The differences in action and response to analgesics are due in these cases to hyperfunctional or nonfunctional uni-nucleotide gene polymorphisms encoding enzyme-modified transport proteins or receptors involved in the biotransformation and dynamics of analgesics. Genomic testing increases therapeutic efficacy and avoids adverse effects especially in patients with long-term therapies.
 
THE EFFECT OF ZnO NANOPARTICLES ON THE ACTIVITY OF ANTIOXIDANT ENZYMES AND CAROTENOID CONTENT AT RHODOSPORIDIUM TORULOIDES CNMN-Y-30 YEAST
Get PDFThe present research paper provides new information on the influence of ZnO nanoparticles (ZnO NPs) of size <50 nm and <100 nm on Rhodosporidium toruloides CNMN-Y-30 pigmented yeast. It was established that the activity of antioxidant enzymes such as catalase, superoxide dismutase and content of carotenoid pigments in the studied strain has been modified depending on the size and concentrations of NPs. There were no significant differences between the activity of antioxidant enzymes and content of carotenoid pigments in experimental group and control at the use of significantly low concentration of ZnO NPs. The use of nanoparticles in concentration of 30 mg/l caused a decrease in activity of antioxidant enzyme catalase and contributed to the increase in the activity of superoxide dismutase. This study has revealed that the concentration of 30 mg/L of ZnO NPs initiates an significant decrease in the content of carotenoid pigments - β-carotene, torulene and torularhodin in cell biomass. The results provided opportunities for modeling cell cycle processes and highlighting of carotenoid pigments and antioxidant enzymes as parameters for determining the mode of action of nanoparticles.
 
SITOSTEROLEMIA – RARE CAUSE OF HYPERCHOLESTEROLEMIA IN CHILD
Get PDFSitosterolemia is a rare autosomal recessive disorder caused by mutations in ABCG5 or ABCG8, leading to increased intestinal absorption and to a diminution of the biliary excretion of plant sterols (sitosterol, campesterol, stigmasterol) and cholesterol. We report a sitosterolemia case of a little girl with severe hypercholesterolemia at the age of 11 months. The occurence of the some linear xanthoms at the level of the Achilles tendon, bilaterally and the extremely high cholesterol (total cholesterol: 949.6 mg%, LDL-cholesterol: 837 mg%) have constituted the starting point for subsequent investigations. Cardiac and abdominal ultrasound does not have pointed out the changes. No family history of hypercholesterolemia has been reported. Completing genetic tests confirmed the diagnosis of sitosterolemia. By sequencing the entire genome two mutations were detected in the ABCG5 gene (Q16X and R446X). We present this clinical case due to the rarity and particularity of the disease. Genetic tests play the most important role in diagnosing the disease and in an appropriate therapeutic approach for the child patient. Proper diet and family responsibility is the key to dispensing the case and to avoiding complications
TEXTILE DYE BIOREMEDIATION POTENTIAL OF SOME RHIZOBIAL STRAINS AND THEIR HEAVY-METAL AND HIGH SALINITY TOLERANCE
Get PDFThe discharge of untreated textile dye effluents enriched with toxic pollutants including dyes, heavy metals and other hazardous materials may cause negative impacts on the entire ecosystem. The proposed work aimed to isolate, molecularly identify and characterize the native rhizobial strains with textile dye biodegradation potential in relation with their tolerance to high salinity and heavy metals (usually meet in high concentrations in the textile dye effluents). Native rhizobial strains were isolated from various terrestrial ecosystems originated in Danube – Delta Biosphere Reserve. Most of the strains tolerated ≥ 2.0% NaCl. Our data showed that 3 strains (Agrobacterium sp.CR-B19; Rhizobium giardinii CR- B22 and Ensifer sp.CR-B26) were able to tolerate 15 ppm concentration of cadmium (Cd2+), whereas all strains identified as Rhizobium sp. (except R. leguminosarum CR-B10), and Agrobacterium sp. could tolerate 70 ppm of chromium (Cr6+).. Moreover, 3 indigenous strains (Rhizobium giardinii CR-B13; Rhizobium sp.CR-B15 and Agrobacterium sp. CR-B19) tolerated a concentration of 200 ppm of lead (Pb2+). In regard to azo-dye degrading potential, only Rhizobium leguminosarum CR-B10 was able to degrade the Reactive Orange 16 dye (90.18% decolorization) in stationary conditions, at 30°C. Comparatively, Agrobacterium sp. CR - B19 strain removed Reactive Orange 16 (sulphonic azo-dye) (78.92 % decolorization) and Reactive Blue 4 (antraquinonic dye) (12 % decolorization) by adsorbtion. Based on their bioremediation potential, the newly isolated rhizobial strains could be further used (in pure culture or in consortia) to develop a new environmental friendly and cost–effective biotechnology in order to reduce the toxicity of textile dyes effluents
Aspects of the benign extrasystolic arrhythmia in the child and teenager with tetany
Get PDFThe tetany in children represents a state of pathological hyperexcitability of the central and peripheral nervous system. The aim of this study is to present aspects of the benign extrasystolic arrhythmia in the children and teenagers with tetany, starting from the assumption that electrolyte imbalances of calcium and magnesium ions might be the cause of these dysrhythmias. The patients with hypocalcemic and hypomagnesemic tetany had the greatest share, followed by the patients with normocalcemic and normomagnesemic tetany, with no statistically significant difference between the atrial, respectively ventricular extrasystoles in any of the forms of tetany. The percentage of the occurrence of extrasystoles in the patients with latent tetany was higher than in the patients with manifest tetany, with a significant statistical difference between the types of extrasystoles, in both types of tetany
PREVENTION WITH DESORGESTREL OF ENDOMETRIOUS CYSTS RECURRENCE AFTER SURGICAL TREATMENT
Get PDFCurrently, endometriosis is an important public health issue due to the increasing number of cases and the risk of developing ovarian and endometrial tumours(10% of ovarian endometrial carcinomas are associated with uterine endometrioid carcinomas), which has led to increased research into the mechanisms of appearance and progression of this condition.Although endometriosis is considered a benign chronic disease, it can show an invasive character causing severe symptoms. Endometriosis has an increasing incidence, affecting 2% of the general population, being the third gynecological cause of infertility, chronic pelvic pain, dyspareunia and dysmenorrhea. Endometriosis affects 7-15% of women of childbearing age, with 25-30% of sterility cases having a significant social impact. Endometriomas are difficult to excise due to vascularization and increased adhesion of the cyst wall. Surgical treatment of endometriosis is effective in the short term. Surgery most often reduces ovarian reserve. The rate of recurrence after surgery can be reduced with the help of menstrual suppression medications such as hormonal contraceptives. Desogestrel treatment improves intraoperative conditions mainly by reducing bleeding and increasing laxity between cyst coat and normal ovarian tissue
THE EFFECTS OF HORMONAL THERAPY IN MENOPAUSE
Get PDFAbstract. Through all the immediate and long-term consequences of estrogenic deprivation, menopause is an important public health issue with social, personal and economic impact. If immediate menopausal symptoms are sometimes noisy, the long-term, insidious consequences, especially cardiovascular and bone, are of major importance. In order to eliminate troublesome symptoms and prevent long-term consequences in order to provide a good quality of life for menopausal women, it is nowadays unanimously recognized that hormonal therapy is necessary in the context of adhering to recognized indications and contraindications, also rigourosly following the effects of therapy
Targeting the Ether-à-go-go ion channels in cancer therapy: current knowledge and future perspectives
Get PDFMembers of the Ether-à-go-go (Eag) group of potassium channels, including the human Ether-à-go-go (hEag) and the human Ether-à-go-go-related gene (hERG) ion channels, have been shown to play important roles in cancer pathogenesis and to regulate many aspects of tumour development. It was shown that they are frequently overexpressed or ectopically expressed in different human cancers, which, combined with their cell surface expression, led to different attempts at finding their therapeutic potential as promising cancer therapy targets. This review summarizes the current knowledge drawn from the results of independent studies performed to identify sought-after alternatives of targeting these ion channels in cancer therapy