Journal of Experimental and Molecular Biology
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Gheorghe Mustață about The University Professor PhD, Costică Misăilă from Vrancea – At The Retired Age
Get PDFI am also from Vrancea, a so-called vrâncean , but I was born in Adjudu-Vechi, on the Siret bank in Putna County, with great historical resonances, today in Vrancea County; here lived the old man Ion Roată and the fearless sons of Vrancioaia , who honored the homeland, the customs and the people. The biggest city of my childhood was Adjud , or New-Adjud, where I went to the fair every year on the 6thof August, the day of the Transfiguration of Jesus Christ, then during the gymnasium when I commuted a distance of more than 4 kilometers
Vlad Artenie about The University Professor PhD, Costică Misăilă – at the 70th birthday
Get PDFIn August 2018, The University Professor PhD Costică Misăilă celebrates his 70th birthday, alongside all his dear ones, family, friends and peers
IN VITRO STABILITY AND ANTIOXIDANT POTENTIAL OF THE NEUPROTECTIVE METABOLITE 6-HYDROXY-NICOTINE
Get PDFAlzheimer’s disease is complex, and it is unlikely that any one single drug or intervention can successfully treat it. The implication of nicotinic acetylcholine receptors (nAChR) in Alzheimer’s disease pathogenesis has opened a new perspective on finding drugs usable for ameliorating the memory problems associated with AD. By using nAchR modulators, the availability of nAChRs for acetylcholine will be increased and the loss of forebrain cholinergic neurons associated with AD might be overcome. The microbial metabolite 6-hydroxy-D-nicotine has the ability to sustain spatial memory in rats by lowering the levels of oxidative stress in the brain and modulating the nAChRs function probably by binding to a specific site. Its success from a lab chemical to drug depends highly on its stability or shelf life. We have shown that 6-hydroxy-D-nicotine have a more potent antioxidant effect than nicotine when measured as FRAP units. Also, the pH stability of 6-hydroxy-D-nicotine is similar to nicotine, the compounds withstanding 48h exposure to pH above 3 up to 9 at room temperature
STUDY ON PHYTIC ACID CONTENT IN SOME ROMANIAN CEREAL SEEDS
Get PDFThe phytic acid content of the flour and bran from seeds, belonging to four cereal species, as well as the effect of thermal treatment on this acid was the purpose of this paper. The biological material was represented by dried seeds (moisture content 10%) of twelve Romanian cultivars (c), belonging to four cereal species: wheat (3 c), rye (3 c), oat (3 c) and maize (3 c). The seeds of each species were ground to get integral flour and bran. The phytic acid content was analyzed, using Garcia-Villanova method, in the flour and bran, before and after thermal processing (180°C for 50 minutes). They also determined pH values of the integral flour and bran in the four cereal species. Analyzing the phytic acid in integral flour and bran of raw samples, the highest and close values were in rye and maize, and the least ones in wheat and oat. Thermal processing of the both products (flour and bran) led to highest and significant reducing percent of phytic acid in rye, followed by oat and wheat samples with close values, the least reducing percent of phytic acid being in maize samples
INTAKE AND BLOOD CONSTITUENTS OF RED SOKOTO GOATS FED TOTAL DIETS CONSISTING OF HAY MEAL AND PROCESSED BY-PRODUCTS
Get PDFThe intake and blood constituents of Red Sokoto (RS) goats fed total diets consisting of Andropogon tectorum hay meal and two agro-industrial processed by-products; brewer’s dried grains (BDG) and malted sorghum sprouts (MSP) were evaluated. Four diets designated T1, T2, T3 and T4 were formulated to contain varying proportions of BDG and MSP (40:10, 30:20, 20:30 and 10:40). The diets were offered to twenty-four (24) RS goats in a completely randomised design (CRD) experiment for 56 days. Daily feed intakes (g/d) and weekly body weight (kg) changes were measured. Blood samples were collected via jugular vein puncture before and after the experiment for analyses. All the parameters on intake and body weight changes were non-significant (P>0.05); though goats on T4 showed numerical superiority among the other treatments. Apart from the mean corpuscular volume (MCV), white blood cells (WBC) and direct bilirubin which were statistically affected (P<0.05) before the introduction of the experimental diets, all the other blood constituents measured were statistically similar (P>0.05). After the trial, the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), MCV, mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and WBC showed significant differences (P<0.05) among the treatment means while the differential WBC counts were not affected (P>0.05). Goats on T3 and T4 had similar (P>0.05) PCV and Hb values (32.23%; 31.74% and 11.46g/dl; 11.51g/dl). The PCV of goats on T1 (28.99%) and T2 (28.29%) were statistically similar (P>0.05) while their Hb values (9.06g/dl and 9.93g/dl) differed (P<0.05). The WBC counts followed similar trend as the PCV values across the treatments with a range of 12.30×103/μl – 16.95×103/μl. RBC counts were superior for goats on T4 (18.50×106/μl) than the other groups. Blood glucose levels, total bilirubin and creatinine values remained statistically unchanged while blood urea and direct bilirubin values were significantly different (P<0.05) among the treatment means. The glucose values ranged from 58.56mg/dl (T1) – 63.48mg/dl (T2). Higher urea (19.85mg/dl) was recorded for goats on T3 followed by goats on T4 (17.49mg/dl) while goats on T1 had the least urea value (15.22mg/dl). From these findings, it could be deduced that the four dietary treatments did not alter performance and health status of the animals with T4 being adjoined as the best; hence, the use of these agro-industrial processed by-products to support production during periods of forage scarcity should be adopted
Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles
Get PDFThe paper provides new information on β-glucans biosynthesis capacity and the morphological features modification of cells and colonies of Saccharomyces cerevisiae CNMN-Y-20 yeast strain known as producer of β-glucans. It was found that the development cycle and bioproductive capacity of the yeast was affected by cultivation in the presence of ZnO in a concentrations and the contact duration manner. Within 6-24 hours, the reproduction of the nanomodified cell was decreased compared to the control, but after 120 hours of submerged cultivation an insignificant increase in biomass content relative to 1L culture medium was recorded predominantly related to nanoparticle concentrations 0.5-1.0 mg/L. ZnO nanoparticles (<100 nm). Concentrations of 0.5-15 mg/L initiated a 13-15% increase in average dimensions of Saccharomyces cerevisiae CNMN-Y-20 cells and 7-12% increase in the ability to form of β-glucans, especially at concentrations of 1 and 5 mg/L. The correlation coefficient between the cells area and the β-glucans amount is strong (R² = 0.8021). The results provide the possibility of enhancing of the range of analyzes and the formation of reference bases necessary for the strategy to enlarge the biotechnological performance of yeasts