Journal of Experimental and Molecular Biology
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Relationship Between Pattern of Fingerprints and Obesity
Get PDFThe goal was to research the pattern of fingerprints in all the fingers of both hands and to study the association between obesity and fingerprint among university students in different faculties of Koya University with statistical analysis. Dactylography or the fingerprint system relies on the study of stratum ridges and their configurations [dermatoglyphic (derma = skin+ glyph = carving)] in the fingers, palms, and soles. Estimates that probabilities are about one in sixty-four thousand million for two individuals with similar finger impressions. Arbitrate heredity and environment in combination affect the pattern of ridges. We have conducted a study with 120 individual (30 males and 30 females normal and obesity) having the different weight of (normal and obesity), this study was carried out in different faculties in Koya University. All the 10 fingerprint patterns were divided into loop, whorl, and arch. The fingerprint was taken with the help of a stamp pad imprinting the fingerprint ridges over A4 size white paper.The general distribution of the pattern of fingerprint showed high frequency (58.41%) of the loop, whereas whorls were moderate (37.83%) and arches were least (3.75%) in frequency. Loops are dominated in both normal and obesity for both individual males and females. The study suggests an association between fingerprint pattern and obesity ( whorls in left hand of male and female, and arches in different finger of right and left hand of male, also whorls in different finger of right and left hand of female, and arches in different finger of right hand of female) but there is no association between fingerprint pattern and obesity in (loops, whorls, arches, among subject normal and obesity male right hand, loops, and arches among subject normal and obesity of male in left hand, also there is no association between loops, whorls, arches among subject normal and obesity of female right hand, and loops and arches among subjects normal and obesity of female in left hand, also loops and whorls in different finger of right and left hand of male, then loops in different finger of right hand of female, and also loops and arches in different finger of left hand of female) based on statistical analysis of chi-square test when results combined between both genders
Mukherjee Siddhartha, Gena: o istorie fascinantă. Editura “ALL”, 2018, București,
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CANDIDIASIS IN PREGNANCY- PERSONAL STUDY
Get PDFThe impact of an intensive educational program regarding candidiasis in pregnancyon health professionals knowledge at Clinical Hospital of Obstetrics and Gynecology ”Elena Doamna” in Iași, Romania. The study was designed in three phases: Assessment phase, Implementation phase and Evaluation phase. The study was conducted from early January to the end of December 2019. The result of the study shows that its most frequent location is in the mouth and the vagina. The symptoms are reduced and the diagnosis is based on the clinical examination, the confirmation being performed by microscopic examination. Prophylactic treatment of candidiasis involves maintaining a rigorous hygiene, avoiding excessive and unprotected use of antibiotics and increasing the body's immunity through a balanced diet and through the intake of mineral salts and vitamins
FINISHING THE JOB - UTILITY OF LONG-READ SEQUENCING USING THE MINION FOR BACTERIAL GENOMICS
Get PDFSequencing technologies have evolved dramatically since the first two bacterial genomes were published. Currently, due to second generation sequencing, millions of bacterial genomic sequences exist, although a significantly smaller amount represent completely assembled genomes. Third generation sequencing allows the analysis of single molecules, with read-lengths that cover highly complex repetitive regions previously inaccessible by short-read sequencing. However, long-read sequencing is known for producing errors which make long-read-only genome assemblies unreliable or complex if high accuracy is important for further applications. Here, Oxford Nanopore Technology’s MinION, the first handheld nanopore sequencing device, is evaluated in comparison with competing sequencing platforms. The MinION’s applications, potential and limitations are reviewed, focusing on its utility for bacterial genome de novo or hybrid assembly.  
Micronucleus Test In Epithelial Cells From Oral Cavity In Koya University student Somokers And Non- Somokers
Get PDFThis work aimed to investigate the use of epithelial cells from the oral cavity in identifying smoking-related effects in male smokers, normal male, normal female. To establish the relationships between micronucleated cell, binucleated cell, condensed chromatin cell. A total of 59 subjects, corresponding to 11 normal males, 19 normal female, 29 male smokers were registered for this study. The buccal epithelial cell was selected because of the direct exposure of tobacco smoke. We appraised the incidence of micronucleus formation from 29 male smokers and who had smoked a minimum of 1 pack-year and a maximum of 12. Because of their increased smoke intake, male smokers group showed high buccal micronuclei frequency, significantly P<0.05 increased micronucleus frequency was observed in the male smokers group. Micronuclei are cytoplasmic chromatin mass with the appearance of small nuclei that arise from chromosome fragments in the anaphase stage of cell division. Their presence in cells is a reaction of structural and numerical chromosomal aberration arising during mitosis. In an analysis of the frequency of Binucleated cell in 29 male smokers, 11 normal male statistically non-significant differences were noted. The average frequency of condensed chromatin cell in 11 normal male and 29 male smokers were high P<0.05, this is statistically significant and there is a relationship between smoking and increasing in condensed chromatin cell as we mentioned before smoking leads to cytogenetical damage to the human buccal epithelial cell.
 
A SENSITIVE METHOD FOR SALIVA DETECTION IN FORENSICS USING SALIVARY AMYLASE COUPLED WITH AMPLEX RED OXIDATION
Get PDFA new sensitive method for saliva detection was developed, based on salivary amylase detection but with a final fluorescent product, which increases its sensitivity. After the starch is degraded due to the presence of salivary amylase, glucose is oxidised and generates hydrogen peroxide which is able to transform Amplex Red in resorufin - a highly fluorescent product. The final product is visible both under normal and UV light. The method is fast, accurate, can detect trace amounts of saliva and shows little to no interference with other body fluids. A further increase in sensitivity could be obtained by using horseradish peroxidase in the final step, but this would also lead to an increased background signal and stronger interference with urine
THE EPIDEMIOLOGICAL CHARACTERISTICS OF CHILDREN’S POISONING WITH HOUSEHOLD SUBSTANCES - RETROSPECTIVE STUDY: poisoning with household substances
No full textPoisoning with household substances is responsible for a significant morbidity in children. The objective of this study is to specify the epidemiological characteristics of accidental and voluntary poisoning with these products at pediatric age. Research Design and Method.The study was observational, retrospective and included acute intoxication by ingestion of household substances in children aged 0-18 years hospitalized in The Regional Toxicology Center of the Emergency Hospital for Children "St. Maria “Iasi. Results.The study group included 230 children admitted in the last 6 years with this diagnosis. Data were obtained from patient observation sheets, centralized and subsequently processed statistically. Conclusion.Knowing the epidemiological aspects of these poisonings can contribute to the elaboration and application of appropriate prevention strategie
Book of Abstracts - New trends in Biology: from molecules to complex systems, 28 - 29 October 2021, Iași, Romania
No full textThis volume is a compilation of the scientific work abstracts that have been presented in the Scientific Session of the Faculty of Biology (SSFB), New Trends in Biology: from Molecules to Complex Systems, successfully held on October 28th-29th, 2021
A perfect educator and a special person departed from our midst – PhD Professor Vlad Artenie (1936 -2020)
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TOTAL POLYPHENOLS, FLAVONOIDS CONTENTS AND ANTIOXIDANT ACTIVITY OF ROSA SP. GENOTIPES FROM DIFFERENT ALTITUDE OF ROMANIAN REGIONS
Get PDFEight wild rose hip genotypes from different altitudes varying from 3m to 902m were analyzed in order to evaluate the total polyphenols, flavonoids content and the antioxidant activity. The Rosa spp.rosehips collected from the Northeastern and the Southeasternof Romanianwere, as follows: Rosa canina, R. caesia, R. corymbifera, R. micrantha, R. nitidula, R. rubiginosa, R. subcanina, and R. vosagiaca. In some genotypes, the level of flavonoids and polyphenol content increased with the increasing altitude while in other it was observed a decrease. Polyphenol content reached a maximum of 144.36 mg GAE/g DW in R. rubiginosa whereas the lowest content of 61.72 mg GAE/g DW was recorded in R. caesia. The highest polyphenol content was reached at altitude of 860 m. The amount of flavonoids content ranged between 7.32 mg CE/g DW and 19.45 CE/g DW in R. caesia and in R. nitidula, respectively. The radical scavenging capacities of Rosa genotypes extracts were not positively correlated with altitude, except the R. corymbifera extractswhere the antioxidant activity increased with the increase of altitude