Osaka Dental University Academic Repository / 大阪歯科大学学術リポジトリ
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Bone regeneration using three-dimensional spheroids derived from dedifferentiated fat cells.
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Development of a new self-cleaning denture base material using titanium apatite.
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Anesthetic management with remimazolam for adolescent mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS): a case report.
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Can online interactions reduce loneliness in young adults during university closures in Japan? The directed acyclic graphs approach.
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Combined external radiotherapy and single-fraction palliative high-dose-rate interstitial brachytherapy for a patient with a base of tongue cancer who had a previous radiation history.
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Early introduction of oral feeding and its impact on full oral feeding and discharge in preterm infants: a systematic review protocol.
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Evaluation of temporomandibular joint osteoarthritis using a new FRACTURE sequence of 3.0T MRI.
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Identifying key factors in papilla growth around implants: Focus on intraoral negative pressure.
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Autologous Fibrinogen Glueの添加がサンゴ顆粒を用いた抜歯創の治癒に与える影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）In orthodontic treatment, the use of bone replacement material can improve effectiveness of treatment by preventing delayed healing of tooth extraction wounds and bone loss in the extraction wounds. Cells, scaffolds, and growth factors are considered crucial three factors for bone regeneration. In this study, as conditions for bone reconstruction of tooth extraction wound, coral granules were used as a scaffold and autologous fibrinogen glue (AFG) obtained from plasma was used as a growth factor. The extraction wounds experimentally prepared in the mandibles of a beagle dog filled with AFG and coral granules (CG) served as the experimental group and those filled with CG served as the control group. Microfocus X-ray CT images and histopathological samples were observed at 4 and 12 weeks after filling, and plaster casts at the time points immediately after extraction, 4 weeks, and 12 weeks after filling were made to evaluate changes in length of alveolar. At 4 weeks after filling, new bone formation was observed in the control group, while new bone increase was observed in the experimental group. At 12 weeks after filling, the control group showed that new bone and bone marrow formation in the extraction wounds and that CG was barely present.
On the other hand, the experimental group showed that bone reconstruction and bone marrow formation at all layers of the extraction wounds. Therefore, it is suggested that the filling of CG with AFG in extraction wound promotes healing and is effective for bone reconstruction.doctoral thesi
Fusobacterium nucleatum 由来の細胞外膜小胞が神経分化に及ぼす影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）The periodontopathogenic bacterium Fusobacterium nucleatum (F. nucleatum) has attracted significant attention because of its association with many systemic diseases. We investigated the effect of periodontopathogenic outer membrane vesicles (OMVs), which function as a virulence factor of F. nucleatum, on neuronal differentiation. The effect of OMVs on neuronal growth factor (NGF)-differentiated pheochromocytoma cells (PC12 cells) was examined using a tetrazolium dye microculture assay. The differentiation of PC12 cells treated with NGF and/or OMVs was subsequently observed under a microscope. After incubating NGF-differentiated PC12 cells with OMVs, we analyzed the expression of phosphorylated cAMP response element-binding protein (p-CREB), phosphorylated glycogen synthase kinase 3β (p-GSK3β), phosphorylated extracellular signal-regulated kinase (p-ERK), phosphorylated c-Jun N-terminal kinase (p-JNK), and phosphorylated p38 (p-p38). OMVs inhibited PC12 cell proliferation and differentiation. NGF promoted p-CREB expression. OMVs inhibited NGF-induced enhancement of p-CREB expression. NGF also enhanced p-GSK3β, p-ERK and p-p38 expression, but not p-JNK expression. OMVs suppressed the NGF-enhanced expression of p-GSK3β, p-ERK, and p-p38.
This study showed that F. nucleatum OMVs inhibits the proliferation and differentiation of PC12 cells, and that this inhibition is mediated in part by the downregulation of p-CREB expression. Furthermore, the inhibition of p-CREB expression was partially mediated by the suppression of p-GSK3β, p-ERK and p-p38 expression. These results suggest that F. nucleatum OMVs may suppress neuronal proliferation and differentiation.doctoral thesi