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純チタン金属への大気圧プラズマ処理が血管内皮細胞の初期挙動に与える影響について
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Early osseointegration is important for achieving initial stability after implant placement, and our collaborators used atmospheric pressure plasma treatment as one of the methods to make the titanium surface super hydrophilic. According to the researchers, this was useful for improving the initial adhesion of rat bone-marrow cells and their ability to induce hard-tissue differentiation without changing the properties of the material surface. Therefore, in this study, we compared and investigated the effects of atmospheric pressure plasma treatment of titanium surface on the behavior of rat vascular endothelial cells (ECs). The experimental material was a JIS grade 2 titanium surface. The untreated surface served as the control group, and the one irradiated with atmospheric pressure plasma via a piezo brush served as the experimental group. X-ray photoelectron spectroscopy (XPS) was used to analyze the elemental composition of the sample surface. Furthermore, the contact angle of distilled water on the surface of each group was 3measured. Subsequently, ECs were isolated from the descending thoracic aorta of 8-week-old male SD rats and cultured in primary cultures. The number of cell adhesions between the experimental and control groups and the expression of genes related to the initial adhesion of ECs were measured 2 and 5 days after the culture began. Student’s t-test was used for statistical analysis on various measured values, with the significance level set to 5% or less. In the experimental group, XPS observations revealed a decrease in carbon peak intensity and hydroxide formation. When the cell adhesion numbers were compared, the experimental group exhibited significantly higher values at all measurement times. Furthermore, the experimental group exhibited significantly higher gene expression values, indicating an initial adhesion to the material surface at all measurement times. When compared to the control group, fluorescent microscopic images revealed that the experimental group had longer and more widely attached cell processes. These findings indicate that atmospheric-pressure plasma treatment of titanium is beneficial for improving EC adhesion and gene expression of adhesion factors.doctoral thesi