Osaka Dental University Academic Repository / 大阪歯科大学学術リポジトリ
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A serum creatinine-cystatin C-derived index as a predictive marker for sarcopenia incidence and progression in community-dwelling older adults: a prospective cohort study.
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Association of oral frailty with medical expenditure in older Japanese adults: The study of late-stage older adults in Tottori (START Tottori).
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口腔内スキャナーによる歯列を含む口腔周囲スキャンデータを使用した3次元仮想患者表現の精確さに関するin vitro研究
Get PDF大阪歯科大学Osaka Dental University博士（歯学）This in vitro study investigated the effect of skin markers on the accuracy of three-dimensional virtual patient reconstructions created using an intraoral scanner (IOS). A digital reference model (DRM) was fabricated by scanning the mannequin head with a maxillary dentition model. Perioral scans, including dentition (POSD), were acquired using two IOS systems, Medit i700 (Medit, Seoul, Republic of Republic of Korea) and Trios 4 (3shape A/S, Copenhagen, Denmark), both with and without skin markers. The nasal-glabellar region, an area relatively stable during facial movement, was used to integrate POSD data with facial data and maxillary dental models to create digital experimental models (DEMs). Accuracy was evaluated by measuring the three-dimensional positional deviations of the maxillary arch between the DRM and DEMs. The results demonstrated a significant interaction between IOS type and skin marker use. While both IOS systems showed clinically acceptable accuracy, the Medit i700 exhibited a significant improvement in accuracy when skin markers were applied, unlike the Trios 4, which showed minimal difference. This suggests that the effectiveness of skin markers is influenced by the specific scanning technology and algorithms of each IOS.doctoral thesi
ゼラチン不織布を用いた間質細胞由来因子-1の徐放化による骨再生挙動の解析
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Objective of this study was to explore the effect of controlled release for stromal cell-derived factor-1 (SDF-1) on mobilization activity of mesenchymal stem cells (MSCs) and bone regeneration behavior. A gelatin nonwoven fabric (GNF) was selected as the controlled release carrier of SDF-1. After in vitro evaluating the SDF-1 release profile and migration activity for rat MSCs, the GNF incorporating SDF-1 was implanted into the critical-sized cranial defect. Histological analysis showed that the MSC mobilization activity of GNF incorporating SDF-1 was higher than that of GNF alone. Nevertheless, the controlled release of SDF-1 from GNF failed to induce sufficient bone regeneration.doctoral thesi
環境pHが口腔微生物叢の構造と多様性に与える影響：in vitro研究
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Although in vivo studies have explored the relationship between oral environment and microbial communities, a precise analysis of the effects of environmental pH on oral microbiota remains challenging owing to their complexity and external influences. This study aimed to investigate
the effect of environmental pH on the structure and diversity of oral microbiota in vitro. Tongue coating samples from ten participants were cultured anaerobically at six pH levels (5.5–8.0) using adjusted media. After DNA extraction, high-throughput sequencing of the 16s rRNA V3–V4 region was performed. Microbial diversity was analyzed using alpha and beta diversity indices, and differential taxa were identified using linear discriminant analysis effect size (LEfSe). Alpha diversity analysis revealed reduced diversity at pH 5.5 and 8.0. Beta diversity revealed that microbial
communities at pH 5.5, 7.5, and 8.0 were separated from those at pH 7.0. LEfSe identified bacterial species that were significantly altered at pH 5.5 and 8.0. Environmental pH significantly influences the diversity and composition of microbial communities, with substantial changes occurring under acidic or alkaline conditions. These findings provide deeper insight into how oral biofilms respond to pH variations, underscoring the critical role of pH in oral microbiota dynamics.doctoral thesi
3Dプリントシステムと造形角度が義歯床の精確さに及ぼす影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Purpose: This study evaluated the effects of two 3D printing technologies and three orientations on the accuracy of 3D-printed complete dentures. Additionally, whether differences in resin affect the accuracy of denture bases under varying printing systems and orientation conditions was examined.
Methods: The denture bases were printed using digital light processing (DLP) and liquid crystal display (LCD) at three orientations (0°, 45°, and 90°) with two photopolymer resins (n = 6 per condition). Intaglio surfaces were scanned for experimental data. Trueness was evaluated by superimposing experimental data onto master data, while precision was assessed by pairing and superimposing two of six data sets. Deviation analysis was represented as root mean square and color map data. Statistical analysis was conducted using the Kruskal–Wallis method and the Mann–Whitney U test (α = 0.05).
Results: The DLP printer achieved the highest trueness at an orientation of 90°, while precision was minimally affected by orientations or printing systems. DLP printers performed well with both recommended and non-recommended resins; however, LCD printers demonstrated better accuracy with the recommended resins. Both printers at 0° exhibited a negative deviation pattern over the entire palatal surface on the color map data of trueness. The precision data was generally displayed in green on the color map under all conditions, indicating high reproducibility.
Conclusions: The printing system and orientation affected the accuracy of 3D-printed dentures. DLP printers demonstrated superior trueness at 90°. In certain printing system and under orientation conditions, accuracy was affected by different resins.doctoral thesi
超音波ガイド下によるヒト献体を用いて顎関節の上・下関節腔を標的とした選択的穿刺法の確立
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Objective: To investigate an ultrasound-guided selective puncture method for the temporomandibular joint, targeting superior (SJS) and inferior joint spaces (IJS), using a human cadaver.
Study Design: The study utilized five cadavers donated by members of Osaka Dental University. The cadaver's left side was aimed at the SJS, while the right side was at the IJS. Non-ionic contrast was injected into each joint using a crossing technique, with the linear ultrasound probe placed parallel to the long axis of the mandibular branch and punctured perpendicularly into the joint cavity. Cone-beam computed tomography scans assessed both the temporomandibular joints examining discs and other components for damage using a gross anatomical technique.
Result: Computed tomography images confirmed successful contrast agent injection into all joint spaces without damaging the articular discs or cartilage of the mandibular head.
Conclusion: Our findings suggest that the ultrasound device can selectively puncture the temporomandibular joint in the SJS and IJS. In the future, the success rate is expected to improve with additional cadaver punctures. We aim to establish this technique as a viable treatment method that can be performed by oral surgeons with limited clinical experience in cadaver surgery training and surgical technique training program using human cadavers.doctoral thesi
Fusobacterium nucleatum 亜種に対して効果的な殺菌作用を有する赤色光を使用した最適な光線力学療法
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Fusobacterium nucleatum (F. nucleatum), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets F. nucleatum by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on F. nucleatum has not been evaluated previously. This study investigates the effect of ALA-PDT using red light-emitting diode (LED) light on F. nucleatum subspecies and their porphyrin accumulation. F. nucleatum subspecies were cultured with varying concentrations of 5-ALA under anaerobic conditions. Porphyrin accumulation was measured via fluorescence spectroscopy, and colony-forming units were measured to determine bacterial viability post-treatment. Additionally, other subspecies responded well to 0.01% 5-ALA, and uroporphyrin I accumulation correlated with bacterial death, revealing optimal bactericidal conditions. These results suggest that optimizing light intensity and 5-ALA concentration can significantly enhance the therapeutic potential of ALA-PDT in oral healthcare.doctoral thesi
間葉系幹細胞抽出物は皮膚創傷治癒を促進する
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Recently, it has been reported that mesenchymal stem cell (MSC)-derived humoral factors promote skin wound healing. As these humoral factors are transiently stored in cytoplasm, we collected them as part of the cell extracts from MSCs (MSC-ext). This study aimed to investigate the effects of MSC-ext on skin wound healing. We examined the effects of MSC-ext on cell proliferation and migration. Additionally, the effect of MSC-ext on skin wound healing was evaluated using a mouse skin defect model. The MSC-ext enhanced the proliferation of dermal fibroblasts, epithelial cells, and endothelial cells. It also increased the number of migrating fibroblasts and epithelial cells. The skin defects treated with MSC-ext demonstrated rapid wound closure compared to those treated with phosphate-buffered saline. The MSC-ext group exhibited a thicker dermis, larger Picrosirius red-positive areas, and a higher number of Ki67-positive cells. Our results indicate that MSC-ext promotes the proliferation and/or migration of fibroblasts, epithelial cells, and endothelial cells, and enhances skin wound healing. This suggests the therapeutic potential of MSC-ext in treating skin defects as a novel cell-free treatment modality.doctoral thesi
TiO₂含有試作ボンディング材に関する研究-エナメル質に対する接着性ならびNd:YAGレーザーを用いた歯科矯正装置の除去性について-
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Resin attachments in orthodontic treatment are applications of resin bonding technology. However, damage to the healthy enamel resulting from the hurried removal or debonding of resin attachments is a problem. We measured shear bond strength in order to examine adhesion to enamel with an experimental adhesive containing TiO2 (5, 10, or 20 wt%), which is a photoreaction agent. Additionally, we measured shear bond strength after laser irradiation in order to examine the effects of the Nd:YAG laser on debonding of the experimental adhesive containing TiO2. We found that the shear bond strength of the experimental adhesive containing TiO2 to enamel decreased as TiO2 content increased, and that the bond strength of experimental adhesive containing TiO2 was sufficiently high. The shear bond strength of the control groups and the experimental adhesive containing TiO2 decreased as the Nd:YAG laser irradiation time increased. At TiO2 concentrations of 10% and 20%, resin jigs fell off the specimens with laser irradiation. The cohesion failure of enamel was not observed on the fracture surfaces after irradiation of the Nd:YAG laser. The results of this study suggest an effectiveness of the Nd:YAG laser for removing resin attachment using an experimental adhesive containing TiO2.doctoral thesi