Osaka Dental University Academic Repository / 大阪歯科大学学術リポジトリ
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歯根膜線維芽細胞におけるsodium-dependent vitamin C transporter2の役割
Get PDF大阪歯科大学Osaka Dental University博士（歯学）"Aim: Ascorbic acid (AA) is a water-soluble vitamin that has antioxidant properties and regulates homeostasis of connective tissue through controlling various enzymatic activities. Two cell surface glycoproteins, sodium-dependent vitamin C transporter (SVCT) 1 and SVCT2, are known as ascorbate transporters. The purpose of this study was to investigate the expression pattern and functions of SVCTs in periodontal ligament (PDL) and PDL fibroblast (PDLF).
Methods: Gene expression was examined using real-time polymerase chain reaction (PCR) and reverse transcription PCR. SVCT2 expression was determined by immunofluorescence staining, western blot and flow cytometry. ALP activity and collagen production were examined using ALP staining and collagen staining. Short interfering RNA was used to knock down the gene level of SVCT2. Change of comprehensive gene expression under SVCT2 knockdown condition was examined by RNA-sequencing analysis.
Results: Real-time PCR, fluorescent immunostaining, western blot and flowy cytometry showed that SVCT2 was expressed in PDLF and PDL. ALP activity, collagen production, and SVCT2 expression were enhanced upon AA stimulation in PDLF. The enhancement of ALP activity, collagen production, and SVCT2 expression by AA was abolished under SVCT2 knockdown condition. RNA-sequencing revealed that gene expression of CLDN4, Cyclin E2, CAMK4, MSH5, DMC1, and Nidgen2 were changed by SVCT2 knockdown. Among them, the expression of MSH5 and DMC1, which are related to DNA damage sensor activity, was enhanced by AA, suggesting the new molecular target of AA in PDLF.
Conclusion: Our study reveals the SVCT2 expression in PDL and the pivotal role of SVCT2 in mediating AA-induced enhancements of ALP activity and collagen production in PDLF. Additionally, we identify alterations in gene expression profiles, highlighting potential molecular targets influenced by AA through SVCT2. These findings deepen our understanding of periodontal tissue homeostasis mechanisms and suggest promising intervention targeting AA metabolism."doctoral thesi
家兎下顎骨における異種ブロック骨を用いたリングテクニックによるインレーおよびオンレーグラフトの骨新生
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Xenogenous bone has been proposed as an alternative to overcome the disadvantages of autogenous grafting. The aim of the present study was to study bone dynamics at inlay and onlay xenografts used for bone augmentation applying a ring technique. Methods: The bone at the lateral surface of the mandibular angle of 12 adult male New Zealand White rabbits was exposed bilaterally. The cortical layer received multiple perforations on one side of the mandible, and a xenograft block of collagenated cancellous equine bone, 7 mm in diameter and 3 mm in width, was fixed on the prepared surface using an implant (onlay group). On the opposite side, a defect 7 mm in diameter and 3 mm in depth was prepared, and the xenograft block was adapted to the defect and fixed with an implant (inlay group). Results: After ten weeks of healing, in the onlay grafts, new bone was mainly formed on the trabeculae surface, reaching in some specimens the most coronal regions of the block. In the inlay grafts, new bone was found arranged on the trabecular surfaces but also occupying the spaces among the trabeculae. The entrance of the defect was often found close to the top of the block by newly formed bone. A higher percentage of new bone was found in the inlay (19.0 ･ 9.3%) compared to the onlay (10.4 ･ 7.4%) groups (p = 0.031). The mean gain in osseointegration at the implant in relation to the base of the original 3 mm deep defect was 0.95 ･ 1.05% in the onlay group and 0.78 ･ 0.71% in the inlay group (p = 0.603). Conclusion: The inlay grafts exhibited a higher new bone percentage than the onlay block grafts possibly due to the defect conformation that presented more sources for bone growth. The trabecular conformation and the composition of the grafts made possible the expression of the osteoconductive properties of the material used. This resulted, in several specimens, in the growth of bone on the graft trabeculae toward the most superior regions in both groups and in the closure of the coronal entrance of the defects in the inlay group. The clinical relevance of this experiment is that the ring technique applied as an inlay method could be suitable for bone augmentation.doctoral thesi
新規填入用コラーゲン/グリセリン/プルランゲルが骨髄間葉系幹細胞の硬組織分化誘導能ならびにラット頭蓋骨欠損の修復に与える影響
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Bone tissue engineering is a technique that simulates the bone tissue microenvironment by utilizing cells, tissue scaffolds, and growth factors. The collagen hydrogel is a three-dimensional network bionic material that has properties and structures comparable to those of the extracellular matrix (ECM), making it an ideal scaffold and drug delivery system for tissue engineering. The clinical applications of this material are restricted due to its low mechanical strength. In this investigation,a collagen-based gel (atelocollagen/glycerol/pullulan [Col/Gly/Pul] gel) that is moldable and injectable with high adhesive qualities was created by employing a straightforward technique that involved the introduction of Gly and Pul. This study aimed to characterize the internal morphology and chemical composition of the Col/Gly/Pul gel, as well as to verify its osteogenic properties through in vivo and in vitro experiments. When compared to a standard pure Col hydrogel, this material is more adaptable to the complexity of the local environment of bone defects and the apposition of irregularly shaped flaws due to its greater mechanical strength, injectability, and moldability. Overall, the Col/Gly/Pul gel is an implant that shows great potential for the treatment of complex bone defects and the enhancement of bone regeneration.doctoral thesi
脂質異常症マウスの唾液および唾液腺におけるCmpk2遺伝子・蛋白質の発現
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Salivary biomarkers are promising molecules for diagnosing systemic diseases. Cytidine/uridine monophosphate kinase 2 (CMPK2) is associated with various systemic diseases. However, little is known about the role of the CMPK2 gene in saliva and dyslipidemia. This study investigated the relationship between serum lipid levels and Cmpk2 mRNA expression in the saliva of dyslipidemic mice. Additionally, immunofluorescence staining was employed to assess the lo-calization of the CMPK2 protein in the submandibular gland. Two types of dyslipidemic mice were utilized: mice fed a high-fat and high-cholesterol (HFHC) diet and genetically dyslipidemic ApoE-deficient mice. The mice at 9 to 46 weeks were analyzed for serum lipid levels, Cmpk2 mRNA expression in saliva, and CMPK2 protein localization in the submandibular glands. Both dyslipidemic mice displayed elevated low-density lipoprotein cholesterol and total cholesterol in serum. ApoE-deficient mice apparently exhibited increased Cmpk2 expression in saliva. Immuno-fluorescence staining indicated that CMPK2 proteins were primarily localized in the serous acini, potentially associated with the secretion of Cmpk2 mRNA in saliva. These findings suggest that Cmpk2 mRNA increases and is detectable in the saliva of dyslipidemic mice, providing a viable experimental model to assess the potential use of CMPK2 as a biomarker for dyslipidemia.doctoral thesi
Antimicrobial susceptibility surveillance of bacterial isolates recovered in Japan from odontogenic infections in 2018.
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High-dose-rate surface mold with tumor translucency brachytherapy for a 103-year-old patient with skin cancer, dementia, tremor, and kyphosis: a case report.
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最適化された頬脂肪体由来骨誘導性細胞外小胞はヒト脱分化脂肪細胞の骨芽細胞分化を促進する
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Background/purpose: Bone reconstruction in the maxillofacial region typically relies on autologous bone grafting, which presents challenges, including donor site complications and graft limitations. Recent advances in tissue engineering have identified highly pure and proliferative dedifferentiated fat cells (DFATs) as promising alternatives. Herein, we explored the capacity for osteoblast differentiation and the osteoinductive characteristics of extracellular vesicles derived from DFATs (DFAT-EVs).
Materials and methods: DFATs were isolated from human buccal fat pads, cultured to confluency, and placed in either a standard or osteogenic induction medium. After culturing for 3 days, the conditioned medium was used to generate EVs using the size-exclusion chromatography and concentration filter method.
Results: Characterization of DFAT-EVs revealed typical EV morphology and positive markers (CD9 and CD63), with no differences between the two groups. In vitro assays demonstrated that EVs derived from the osteogenic induction medium (OI-EVs) significantly increased alkaline phosphatase activity and osteogenesis-related genes (Runx2 and collagen type I) compared to control EVs. Next-generation sequencing identified differentially expressed miRNAs, and gene ontology analysis suggested pathways involved in osteoblast differentiation.
Conclusion: Isolating DFATs from buccal fat pads under osteogenic induction conditions offers a procedure confined to the oral cavity, eliminating the need for harvesting from other sites. Thus, DFAT-EVs hold promise for promoting bone regeneration in maxillofacial applications.doctoral thesi
家兎における異種骨補填材を用いたインプラント体周囲クリティカルサイズ骨欠損の骨再生について
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Background Healing of critical-size defects is a well-known problem that has been challenged in several studies. The aim of the experiment was to evaluate bone formation and osseointegration of implants installed in critical defects of the mandibular body simultaneously grafted with Bio-Oss® or Cerabone®.
Material and methods Defects, 10 mm wide and 3 mm deep, were prepared at both lateral aspects of the mandible in 12
rabbits. One implant was installed in the center of the defect, and bovine xenografts produced either at low (Bio-Oss®;
Low-T) or high (Cerabone®; High-T) temperatures were used to fill the defects. A collagen membrane was placed to cover
the sites. Healing was evaluated 10 weeks after surgery.
Results In both groups, most sites showed optimal healing with closure of the coronal entrance of the defects. However,
residual defects occupied by soft tissues and biomaterial particles were observed, even though generally limited to some
regions of the defect. Osseointegration of the implant surface in the region of the defect was poor in both groups.
Conclusions Circumferential marginal critical-size defects around implants filled with bovine xenografts presented regions
with a complete healing in both groups. However, the healing was not complete at all regions in most defects; therefore, a
complete optimal healing of critical-size marginal defects cannot be predicted.doctoral thesi
水ようかんの食感を表現するオノマトペと関連する因子の検討
Get PDF大阪歯科大学Osaka Dental University博士（歯学）We investigated oral and systemic factors related to the onomatopoeia of food texture. The subjects were randomly selected from 114 patients between 53 and 89 years of age who visited Osaka Dental University Hospital for routine treatment. Based on a general examination and oral cavity survey, 5 subjects with swallowing problems were excluded. In addition, 79 denture wearers were included in the limited analysis. Patients answered a questionnaire after eating a strained type of soft azuki bean jelly. We examined factors related to food texture using logistic regression analysis, in which the outcome variable was the onomatopoeia "chunky", on a binary scale of "not applicable" and "applicable", and the explanatory variables included age, oral wetness and denture status. When the maxillary palate was covered by the palatal plate and the oral wetness was low, the onomatopoeia "chunky" was perceived to describe the texture of azuki bean jelly.doctoral thesi
上部構造と天然歯間のコンタクト離開に咬合が及ぼす影響についての横断調査
Get PDF大阪歯科大学Osaka Dental University博士（歯学）Purpose: We investigated the relationship between occlusion and interproximal contact loss (ICL) by analyzing occlusal force and load distribution on adjacent teeth (AT) in 144 patients with implant-supported restorations (ISR) and no periodontal disease. ICL was defined as the ability to insert a 110 µm contact gauge without resistance. Methods: Using an occlusal force measurement system, we assessed total occlusal force, force on AT and ISR, and load distribution across the occlusal surface. Results: The ICL incidence was 21.2%, with the highest prevalence when occlusal loads were concentrated distally side on mesial AT. Conclusion: Our findings suggest that distal load concentration on mesial AT contributes to ICL, highlighting the importance of load distribution in preventing ICL in patients with ISR.doctoral thesi