Universitas Setia Budi Surakarta: USB e-journal
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The Profiles of Hepatitis B and C Virus Infections in Prison-Assisted Citizens
Get PDFPrison-assisted citizens or prisoners are one of the groups of people who are at high risk of Hepatitis B and C virus infections. The data on the prevalence of both viruses in prisoners in Indonesia are still limited. This study aims to determine the profiles of Hepatitis B and C virus infections in prison-assisted citizens in the Class IIA Narcotics Correctional Institution in Bandung Regency. A total of 30 prisoners were used as the samples in this study. All procedures performed in this study were following the applicable codes of ethics. The presence of surface antigens of Hepatitis B virus (HBsAg) in serums was detected using a qualitative sandwich Enzyme-Linked Immunosorbent Assay method. The existence of antibodies of the Hepatitis C virus (Anti-VHC) was detected using the immunochromatography method. The laboratory test results have shown five people (16.7%) were positive on the HBsAg test and one person (3.3%) was positive on the anti-VHC test. One case of Hepatitis B and C co-infection was also found in the prison-assisted citizens who were in the Class IIA Narcotics Correctional Institution in Bandung Regency. All the prisoners infected by the Hepatitis B and C viruses used injection drugs, had tattoos on their bodies and never received vaccinations
Deteksi Dan Isolasi Escherichia coli Resisten Ampisilin Yang Diisolasi Dari Peternakan Ayam Di Surabaya, Indonesia
Get PDFThe study was carried out to isolate and detect the resistance of E. coli bacteria from four livestock in Surabaya, Indonesia.
This research used 40 samples of chicken thigh meat. The first step is isolated Escherichia coli bacteria using BGBB media (Brillilliant Green Bile Broth), and then used EMBA (Eosin Methylen Blue Agar) media for confirmatory tests, IMViC tests (Indole, Methyl Red, Voges Praskauer, and Citrat) to identify the enterobacteriaceae bacteria, and performed the gram staining test. Determined the Escherichia coli sensitivity to antibiotic by using disk diffusion method.
Resistance test has resulted for ampicillin against E.coli is 100% resistance in all farms. In this research 13 of 40 samples from chicken meat had positive E.coli. The result from electrophoresis product of PCR said that resistance Escherichia coli had a specific band. Total 13 samples of positive Escherichia coli resistance ampicillin has specific DNA band primary shv with 768 bp in length.Tujuan penelitian ini yaitu untuk mengisolasi dan mendeteksi bakteri E.coli resisten dari empat peternakan ayam di Surabaya, Indonesia.
Penelitian ini menggunakan 40 sampel daging paha ayam. Escherichia coli diisolasi dari sampel dengan menggunakan media BGBB (Brillilliant Green Bile Broth), selanjutnya digunakan media EMBA (Eosin Methylen Blue Agar) untuk uji konfirmasi, dan dilakukan uji IMViC (Indole, Methyl Red, Voges Praskauer, dan Citrat) untuk mengidentifikasi bakteri enterobacteriaceae, kemudian dilakukan pewarnaan gram. Sensitivitas Escherichia coli terhadap antibiotik ditentukan dengan metode diffuse disc.
Uji resistensi dengan metode MHA menghasilkan persentase resistensi E.coli terhadap ampisilin sebesar 100% pada semua sampel. Dalam penelitian ini 13 dari 40 sampel daging ayam positif mengandung E.coli. Hasil dari produk elektroforesis PCR menunjukkan bahwa resistensi Escherichia coli memiliki band spesifik. Total 13 sampel positif Escherichia coli yang resisten ampisilin memiliki band hasil amplifikasi primer DNA spesifik dengan panjang 768 bp
Stabilitas Kimiawi Sirup Ekstrak Etanolik Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.)
Get PDFThe ethanolic extract of hibiscus flower (Hibiscus rosa-sinensis L.) on concentration 1,25% had mucolytic effect (Septyani 2009) with alkaloid as its marker substances. Syrup was suitable because its local effect so mucus will be able to go off easily. The aim of this research was to determined chemical stability of syrup based on optimum formulation by reduction value of alkaloid as its parameter. Hibiscus powder was extracted using maceration method with PE and its residue remacerated by ethanol 70%. Standard curve was made from isolate of alkaloid with various concentration on 5; 2,5; 1,25; 0,625; and 0,3125 μg/μl with regressional equation Y = -2,225 + 16,777 x. Syrup has been made in 3 formulation with various extract concentration on 1,00; 1,25; and 1,50%. Its physical stability including viscosity and decanted easily test has been checked then the chemical stability has been done by heated syrup on increasing term from 40º, 55ºC and 70ºC. The reduction of stability known by reduction of alkaloid value as AUC from densitometer. It was analyzed using Anova two ways followed by Dunnett T3 with significancy (p 0,05). The result showed that increasing term was influence to reduction of alkaloid value but various extract concentrations were not. So it was concluded that syrup with 1,25% extract concentration had the best stability.Ekstrak etanolik bunga kembang sepatu (Hibiscus rosa-sinensis L.) pada konsentrasi 1,25% berkhasiat sebagai mukolitik (Septyani 2009) dengan zat penanda alkaloid. Sirup dianggap cocok sebagai sediaan karena diharapkan berefek lokal sehingga mempermudah keluarnya mukus. Penelitian ini bertujuan untuk mengetahui stabilitas kimiawi dari sirup yang sudah diketahui formula optimumnya dengan parameter penurunan kadar alkaloid yang terbaca pada densitometer. Ekstraksi dilakukan dengan petroleum eter kemudian residu dimaserasi dengan etanol 70%. Kurva baku dibuat dari isolat dengan konsentrasi 5; 2,5; 1,25; 0,625; dan 0,3125 μg/μl dan didapatkan persamaan Y = -2,225 + 16,777 x. Pembuatan sirup menjadi 3 formula konsentrasi ekstrak yaitu 1,00; 1,25; dan 1,50%. Selanjutnya sirup diuji viskositas dan kemudahan dituangnya sebagai uji mutu fisik. Uji stabilitas kimiawi dilakukan dengan memberi panas terhadap sirup pada suhu 40º, 55º dan 70ºC kemudian penurunan kestabilan dapat diketahui berdasarkan penurunan kadar alkaloid total yang terbaca sebagai luas area puncak pada densitometer. Data dianalisis menggunakan Anova dua jalan dilanjutkan uji Dunnett T3 dengan signifikansi (p 0,05). Hasil penelitian menunjukkan bahwa ada pengaruh kenaikan suhu terhadap penurunan kadar alkaloid, sedangkan perbedaan konsentrasi tidak berpengaruh terhadap penurunan kadar alkaloid. Formula yang paling stabil adalah formula dengan konsentrasi ekstrak etanolik 1,25%
Uji Aktivitas Diuretik Ekstrak n-Heksana Daun Ceplukan (Physalis angulata L.) pada Tikus (Rattus norvegicus) Jantan Wistar
Get PDFCeplukan (Physalis angulata L.) empirically have diuretic effect. This study aimed to determined the diuretic effect of n-hexane extract of ceplukan leaves on Wistar male rats. Ceplukan leaves powder were extracted by maceration with n-hexane solvent. Test animal was used 25 Wistar male rats were divided into 5 groups: group I were given CMCNa 0.5% (negative control), group II (positive control) were given furosemide dose 4.32 mg/200 g BW, group III, IV, V were given n-hexane extract suspension dose of 375, 187.5, 93.75 mg/200 g BW respectivelly. The urine volume were measured in hours-6, 12, 18 and 24. Diuretic effect is obtained by calculating the cumulative urine volume and AUC (Area Under the Curve). The data obtained were analyzed by one way ANOVA with a level of 95% significant, then test by LSD (Least Significant Difference).The results showed that n-hexane extract of ceplukan leaves had diuretic effect. The effective dose of n-hexane extract of ceplukan leaves for diuretic effect was dose 375 mg / 200 g BW.Ceplukan (Physalis angulata L.) secara empiris berkhasiat diuretik. Tujuan penelitian ini untuk menentukan efek diuretik ekstrak n-heksana daun ceplukan pada tikus jantan Wistar. Serbuk daun ceplukan diekstraksi secara maserasi dengan pelarut n-heksana. Hewan uji yang digunakan sebanyak 25 ekor tikus jantan galur Wistar yang dibagi menjadi 5 kelompok, yaitu kelompok I diberi CMC Na 0,5 % (kontrol negatif), kelompok II diberi furosemid dosis 4,32 mg/200 g BB (kontrol positif), kelompok III, IV, V diberi ekstrak n-heksana dosis berurut-turut 375; 187,5; 93,75 mg/200 g BB dengan volume pemberian 2,5 ml/200 g BB. Volume urin diukur pada jam ke-6, 12, 18, dan 24. Efek diuretik diperoleh dengan menghitung volume urin kumulatif dan AUC (Area Under the Curve). Data dianalisis dengan ANOVA satu jalan taraf kepercayaan 95%, kemudian dilakukan uji LSD (Least Significant Difference). Hasil penelitian menunjukkan bahwa ekstrak n-heksana daun ceplukan memiliki efek diuretik terhadap tikus jantan Wistar. Dosis efektif ekstrak n-heksana daun ceplukan untuk efek diuretik adalah 375 mg/200 g BB
Optimasi Proporsi Campuran Carbopol 941 dan CMC-Na dalam Pembuatan Gel Lendir Bekicot (Achatina fullica Ferr.) secara Simplex Lattice Design
Get PDFSnail (Achatina fulica Ferr) mucus efficacious as cure wounds. The use of snail mucus directly considered less practical, so that made the gel preparation. This study aims to obtain the optimum formula of snail mucus gel from carbopol 941 and CMC-Na mixtures using simplex lattice design. Snail mucus obtained by solving the top shell snail then shell reversed for inside snail mucus out. Optimum gel formula based on the simplex lattice design with 2 components, i.e: carbopol 941 (K) and CMC-Na (C) using 3 formulas, i.e: formula I (100% K: 0% C), formula II (50% K: 50% C) and formula III (0% K: 100% C). The physical properties observed were viscosity, adhesiveness and dispersive, which then is used to make the SLD equation. The equation used to make the optimum formula gel from the total response of the most large gel physical nature. Results obtained from the simplex lattice design equation compared with actual test results with t-test (T-test). The results showed an optimum gel formula of snail mucus obtained from of 30% carbopol 941 and 70% CMC-Na mixtures. From the results of t-test (T-tesf) against viscosity, adhesiveness and dispersive showed no significant difference between the predictions with actual experimental results.Lendir bekicot (Achatina fulica Ferr) berkhasiat sebagai obat penyembuh luka. Penggunaan lendir bekicot secara langsung dinilai kurang praktis, sehingga dibuat sediaan gel. Penelitian ini bertujuan untuk mendapatkan formula optimum gel lendir bekicot dari campuran carbopol 941 dan CMC-Na dengan menggunakan metode simplex lattice design. Lendir bekicot diperoleh dengan memecahkan bagian pucuk cangkang bekicot kemudian cangkang dibaiik agar lendir bekicot bagian dalam keluar. Formula gel optimum berdasarkan simplex lattice design dengan 2 komponen yaitu: carbopol 941 dan CMC-Na, menggunakan 3 formula yaitu: formula I (100% carbopol 941 : 0% CMC-Na), formula II (50% carbopol 941 : 50% CMC-Na) dan formula III (0% carbopol 941 : 100% CMC-Na). Sifat fisik yang diamati adalah viskositas, daya lekat dan daya sebar, yang selanjutnya digunakan untuk membuat persamaan SLD. Persamaan tersebut digunakan untuk membuat gel formula optimum dan respon total sifat fisik gel yang paling besar. Hasil yang diperoleh dari persamaan simplex lattice design dibandingkan dengan hasil pengujian sesungguhnya dengan uji-t (T-test). Hasil penelitian ini menunjukkan formula optimum gel lendir bekicot didapat dari campuran 30% carbopol 941 dan 70% CMC-Na. Dari hasil uji-t terhadap viskositas, daya lekat dan daya sebar menunjukkan tidak berbeda signifikan antara prediksi dengan hasil percobaan sesungguhnya
Desain Tempat Sampah Edukatif dan Persuasif Membentuk Karakter Cinta Lingkungan Bagi Anak Usia Dini
Get PDFThe development of educative and persuasive trash design in this study is one of the efforts in early childhood education (golden age), to form the foundation of environmental love character and awareness of disposing of garbage from an early age which is very necessary to be carried to adulthood. As one of the effort to support government programs ini shaping the character attitude and quality of life of human resources, which eventually arises awareness and is willing to move as an effort to preserve, improve and become a long-term environmental safety. To make the design of educative and persuasive trash bins, it is necessary to find the consumer's perception of the object under study, using the Conjoint analysis method. Determination of specific attributes (stimuli) in the development of characteristic specifications divides the concept of education in outline three factors namely auditory, kinestation, visual and developed in several variables. shape: other, design: character, audio: long (10-30 seconds), kinesthetic: automatic.Pengembangan desain tempat sampah edukatif dan persuasif pada penelitian ini merupakan salah satu upaya dalam pendidikan anak usia dini (golden age), untuk membentuk pondasi karakter cinta lingkungan dan kesadaran membuang sampah sejak usia dini yang sangat diperlukan dibawa sampai dewasa. Sebagai salah satu upaya mendukung program pemerintah dalam membentuk karakter, sikap dan kualitas hidup sumber daya manusia, yang akhirnya timbul kesadaran dan mau bergerak sebagai usaha pelestarian, perbaikan dan menjadi keselamatan lingkungan jangka panjang. Untuk membuat desain tempat sampah edukatif dan persuasif perlu dicari persepsi konsumen terhadap objek yang diteliti, dengan menggunakan metode Conjoint analysis. Penentuan atribut (stimuli) secara spesifik dalam pengembangan spesifikasi karakteristik membagi konsep Edukasi dalam tiga faktor secara garis besar yaitu auditori, kinestasi, visual dan dikembangkan dalam beberapa variabel.Berdasarkan hasil kuesioner dengan menggunakan teknik multivariat dapat diketahui bahwa secara umum dari hasil penelitian responden lebih suka bentuk: lainnya, desain: karakter, audio: panjang (10-30 detik), kinestetik: otomatis
Formulasi, Uji kualitas Dan Uji Organoleptik Sabun Mandi Padat Menggunakan Bahan Aditif Daun Jambu Biji (Psidium guajava L.)
Get PDFGuava leaves have flavonoids, tannins, and alkaloids as main compounds which have been shown to be active as antibacterial and anticarcinogenic agents. However, they have never been used as additives in skin care products because they have a rough texture. The purpose of this research are to formulate the method in the production of bath soap with the leaves as additives, and they were examined in quality and organoleptic tests. Formulation in production of the soap was carried out by means of variations of the method of extracting guava leaves as additives, namely: without additive as a control (A), with water solvent (B), with vegetable oil solvent (C), and powder dried as a scrub (D). The soap quality tests based on SNI 2016 including tests for alkaline or free fatty acid levels, insoluble ingredients in ethanol and unsaponified fat fractions. Organoleptic test was carried out involving 20 panelists. Data were analyzed by ANOVA-Duncan test. It was found that soap quality test for all samples meet INS except free alkali levels in D soap. The hedonic test results show that all soap samples can be accepted by panelists. Based on quality and organoleptic tests, the formulation method for adding guava leaf extract as an additive that produces the best soap is extraction using a water solvent (soap B). For the next study, it is necessary to confirm the biological activity of soap with guava leaf additives because it is very potential to be used as an antimicrobial soap.Daun jambu biji mengandung senyawa flavonoid, tanin, dan alkaloid yang telah terbukti aktif sebagai antibakteri dan antikanker. Walaupun demikian, daun jambu biji belum pernah digunakan sebagai aditif dalam produk perawatan kulit karena memiliki tekstur yang kasar. Tujuan dari penelitian ini adalah formulasi produksi sabun mandi dengan variasi metode ekstraksi daun jambu biji sebagai aditif, uji kualitas serta uji organoleptiknya.
Formulasi dalam pembuatan sabun dilakukan dengan cara variasi metode ekstraksi daun jambu biji sebagai aditif, yaitu: tanpa aditif sebagai kontrol (A), ekstrak daun jambu dengan pelarut air(B), ekstrak daun jambu biji dengan pelarut minyak nabati (C), dan serbuk kering daun jambu biji sebagai scrub (D). Uji kualitas sabun berdasarkan pada SNI2016 meliputi uji kadar alkali/asam lemak bebas, bahan tak larut dalam etanol dan fraksi lemak tak tersabunkan. Uji organoleptik dilakukan dengan melibatkan 20 orang panelis. Data dianalisis dengan ANOVA-Duncan test.
Uji kualitas sabun menunjukkan semua sampel memenuhi SNI kecuali kadar alkali bebas sabun D. Hasil uji hedonik menunjukkan semua sampel sabun dapat diterima oleh panelis. Berdasarkan uji kualitas dan organoleptic, maka formulasi metode penambahan ekstrak daun jambu biji sebagai aditif yang menghasilkan sabun terbaik adalah ekstraksi menggunakan pelarut air (sabun B). Kajian berikutnya, perlu dilakukan ujiaktivitas biologis terhadap sabun dengan aditif daun jambu karena sangat potensial digunakan sebagai sabun antimikro
Analisis Biaya Riil Dan Kesesuaian Dengan Tarif INA-CBGs Pada Pasien Kanker Ovarium Di RSUD Dr. Moewardi Surakarta Tahun 2018
No full textOvarian cancer according to the Indonesian Society of Gynecological Oncology ranks second after cervical cancer. Treatment of ovarian cancer is done by surgery, radiotherapy, and chemotherapy. The study aimed to understand the suitability of real costs with INA-CBG rates and the factors that influence real costs in ovarian cancer patients at hospital Dr. Moewardi Surakarta.
The study was taken analytically, the data was taken retrospectively. Subjects were ovarian cancer patients hospitalized at Dr. Moewardi Surakarta in 2018. The analysis used a t-test sample and multivariate analysis to find out research on aid funds with factors related to aid.
The results of the study there were 93 patients, 87 patients included in the classification of chemotherapy and 24 patients included in the classification of surgery. Chemotherapy C-4-13-I class 3 has a difference of Rp-2,685,296, in surgery W-1-20-I class 1 the difference is Rp-3,486,814, for class 3 the difference is Rp -6,933,878, W-1 surgery -20-II class 2 the difference is Rp -6,492,889, grade 3 the difference is Rp -20.450.634, and in surgery W-4-10-III class 2 the difference is Rp -13,714,831. The factor that influences the real cost is LOS (Length Of Stay) (p = 0,000) on chemotherapy. The irregular cycle of chemotherapy leads to the cost of chemotherapy for patient ovarian cancer in Dr Moewardi Surakarta 2018.Kanker ovarium menurut Indonesian Society Gynecologic Oncology berada pada urutan kedua setelah kanker serviks. Pengobatan kanker ovarium dilakukan dengan pembedahan, radioterapi, dan kemoterapi. Tujuan penelitian untuk mengetahui kesesuaian biaya riil dengan tarif INA-CBGs dan faktor-faktor yang mempengaruhi biaya riil pada pasien kanker ovarium di RSUD Dr. Moewardi Surakarta.
Penelitian ini bersifat observasi analitik, data diambil secara retrospektif. Subyek penelitian adalah pasien kanker ovarium yang rawat inap di RSUD Dr. Moewardi Surakarta tahun 2018. Analisis yang digunakan one sample t-test dan analisis korelasi multivariat untuk mengetahua hubungan biaya riil dengan faktor-faktor yang mempengaruhi biaya riil.
Hasil penelitian menunjukkan terdapat 93 pasien, 87 pasien masuk dalam klasifikasi kemoterapi dan 24 pasien masuk dalam klasifikasi pembedahan. Kemoterapi C-4-13-I kelas 3 memiliki selisih Rp -2.685.296, pada pembedahan W-1-20-I kelas 1 selisihnya Rp -3.486.814, untuk kelas 3 selisihnya Rp -6.933.878, pembedahan W-1-20-II kelas 2 selisihnya Rp -6.492.889, kelas 3 selisihnya Rp -20.450.634, dan pada pembedahan W-4-10-III kelas 2 selisihnya Rp -13.714.831. Faktor yang mempengaruhi biaya riil yaitu LOS (Length Of Stay) (p=0,000) pada tindakan kemoterapi. Siklus kemoterapi yang tidak teratur menyebabkan besarnya biaya kemoterapi kanker ovarium pasien di RSUD Dr. Moewardi Surakarta tahun 2018
DETERMINAN KESUKSESAN SISTEM INFORMASI DI RSUD Dr. MOEWARDI
Get PDFThe study of success information system organized is still a study important in researchs systems information. It is caused by existence of the diversity of the results of research on the success of the the information system in organizations on various research an object that impact on variable user and the weapons of the analysis that adapted to the purposes of research (Delone & Mclean, 2003; Wu & Wang, 2006; Holsapple & Le-post, 2006). The purpose of this research is to test antaseden in the form of satisfaction in building intention use the information at RSUD Dr .Moewardi Surakarta. Research sample areas are employees at RSUD Dr .Moewardi as many as 200 respondents. Sampling technique use two stage clusters random sampling, a measuring instrument use testing spss and the testing of hypotheses use SEM (Structural Equation Modelling) with software AMOS. Based on the results of the analysis showed that all hypothesis supported that is significant the connection between satisfaction with the intention to continue, a significant relation between quality information with satisfaction user, a significant relation between quality the system with satisfaction users, and a significant relation exists between the quality of services with satisfaction users information systemThe study of success information system organized is still a study important in researchs systems information. It is caused by existence of the diversity of the results of research on the success of the the information system in organizations on various research an object that impact on variable user and the weapons of the analysis that adapted to the purposes of research (Delone & Mclean, 2003; Wu & Wang, 2006; Holsapple & Le-post, 2006). The purpose of this research is to test antaseden in the form of satisfaction in building intention use the information at RSUD Dr .Moewardi Surakarta. Research sample areas are employees at RSUD Dr .Moewardi as many as 200 respondents. Sampling technique use two stage clusters random sampling, a measuring instrument use testing spss and the testing of hypotheses use SEM (Structural Equation Modelling) with software AMOS. Based on the results of the analysis showed that all hypothesis supported that is significant the connection between satisfaction with the intention to continue, a significant relation between quality information with satisfaction user, a significant relation between quality the system with satisfaction users, and a significant relation exists between the quality of services with satisfaction users information syste
Pengaruh Kelekatan Ayah Terhadap Gejala Depresi Remaja Perempuan Di Kota Bandung
Get PDFAbstract
The purpose of this study was to examine the effect of father-daughter attachment towards adolescent depressive symptoms in Bandung. The sampling technique in this study was purposive sampling with a questionnaire data collection method. The instruments used in this study were the Inventory of Parent And Peer Attachment (IPPA) and Beck Depression Inventory (BDI). The sample in this study were 64 teenage girls in Bandung aged 15-24 years who have experienced three symptoms of depression for at least 2 weeks. The simple linear regression test results show that the higher the father attachment, the lower the depressive symptoms of female adolescents in Bandung. However, the magnitude of this influence is only 13.9%. For future researchers who are interested in investigating further about this variable, it is suggested to include control variables that can contribute to depressive symptoms, such as self-esteem, self-concept, self-efficacy, attributional style, and social support.Abstrak
Tujuan penelitian ini adalah untuk menguji pengaruh kelekatan ayah terhadap gejala depresi remaja perempuan di Kota Bandung. Teknik sampling dalam penelitian ini adalah purposive sampling dengan metode pengumpulan data kuesioner. Alat ukur yang digunakan dalam penelitian ini adalah Inventory of Parent And Peer Attachment (IPPA) dan Beck Depression Inventory (BDI). Sampel pada penelitian ini sebanyak 64 remaja perempuan di Kota Bandung berusia 15-24 tahun yang pernah mengalami tiga gejala depresi selama minimal 2 minggu. Hasil uji regresi linear sederhana menunjukkan bahwa semakin tinggi kelekatan ayah, maka semakin rendah gejala depresi remaja perempuan di Kota Bandung. Meski demikian, besarnya pengaruh tersebut hanya sebesar 13,9%. Bagi peneliti selanjutnya yang tertarik untuk menyelidiki lebih jauh mengenai variabel ini disarankan untuk menyertakan variabel kontrol yang dapat berkontribusi terhadap gejala depresi, seperti self-esteem, self-concept, self-efficacy, attributional style, dan dukungan sosial