Universitas Setia Budi Surakarta: USB e-journal
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House Dust Mites in One of Indonesian Detention Center in 2020
Get PDFHouse dust mite was one of the most common allergens in homes around the world and associated with manifestations of allergies of skin and respiratory tract such as bronchial asthma, allergic rhinitis, and atopic dermatitis. This arthropod was commonly found on carpets, mattresses, children's toys, and other home furnishings. This study was aimed to identify and quantify the number of Dermatophagoides spp in Detention Center. This descriptive study was conducted using flotation method on 52 samples of mattress dust collected in February 2020. The results showed that 49 out of 52 mattress dust samples were positive of Dermatophagoidesspp..Of all Dermatophagoides spp, there were Dermatophagoides pteronyssinus (71.2%) and Dermatophagoides farinae (28.8%). In conclusion, this study found two species of Dermatophagoides spp. in Detention Center and suggested that the fascility maintain the cleanliness of the mattress by drying the mattress and changing the sheets at least once a week
Cytotoxicity Assay of Chitosan-Collagen Wound Dressing using Brine Shrimp Lethality Test Methods
Get PDFWound dressing has a function to protect the wound area from external exposure and provide a moist state around the wound area for accelerating wound healing process. Collagen and chitosan are known as appropriate biomaterials to synthesise of wound dressing because they have anti-bacterial, anti-fungal, biodegradable and biocompatible properties. One of biocompatibility assay for a material is cytotoxicity assay using Brine Shrimp Lethality Test (BSLT) that could be applied before in Vivo assay. The aim of the research was to know the cytotoxicity level of collagen-chitosan wound dressing with variance of concentration such as K0 as control used pure chitosan, K1 used collagen 25% and chitosan 75%, K2 used collagen 50% and chitosan 50%, K3 used collagen 75% and Chitosan 25%. Skin and scales of Gabus fish (Channa striata) were extracted using 2% HCl solvent to obtain collagen and chitosan powder dissolved in 1% acetic acid. Furthermore, wound dressing is made by a combination of collagen-chitosan concentration according to each group (K0, K1, K2 and K3. Citotoxicity assay used Brine Shrimp Level Test (BSLT) method with concentration each sample group were 10, 50, 100, 250, 500, 750 and 1000 ppm. The results showed that each wound dressing group such as K0, K1, K2 and K3 had LC50> 1000ppm that indicated wound dressing was non-toxic.Wound dressing has a function to protect the wound area from external exposure and provide a moist state around the wound area for accelerating wound healing process. Collagen and chitosan are known as appropriate biomaterials to synthesise of wound dressing because they have anti-bacterial, anti-fungal, biodegradable and biocompatible properties. One of biocompatibility assay for a material is cytotoxicity assay using Brine Shrimp Lethality Test (BSLT) that could be applied before In Vivo assay. The aim of the research was to know the cytotoxicity level of collagen-chitosan wound dressing with variance of concentration such as K0 as control used pure chitosan, K1 used collagen 25% and chitosan 75%, K2 used collagen 50% and chitosan 50%, K3 used collagen 75% and Chitosan 25%. Skin and scales of Gabus fish (Channa striata) were extracted using 2% HCl solvent to obtain collagen and chitosan powder dissolved in 1% acetic acid. Furthermore, wound dressing is made by a combination of collagen-chitosan concentration according to each group (K0, K1, K2 and K3. Citotoxicity assay used Brine Shrimp Level Test (BSLT) method with concentration each sample group were 10, 50, 100, 250, 500, 750 and 1000 ppm. The results showed that each wound dressing group such as K0, K1, K2 and K3 had LC50> 1000ppm that indicated wound dressing was non-toxic
Analysis of Interleukin-17 Levels in Patients with Thrombocytopenia
Get PDFThrombocytopenia or platelet deficiency is a condition, in which platelet level in the blood circulation is below normal, which is less than 150,000 cells/µl. Thrombocytopenia is classified into some conditions, including decreased platelet production, increased need for platelets, and other thrombocytopenia. The need for increased platelets can be subdivided into primary immune thrombocytopenia, secondary immune thrombocytopenia, non-primary ITP, and thrombocytopenia that are not immune-mediated. Several cytokines play a role in the process of thrombocytopenia, one of which is Interleukin-17 (IL-17) that will be further discussed in this study. A previous study reported that IL-17 production increased in ITP and cITP patients. The objective of this study was to analyze the IL-17 levels and figure out the differences in IL-17 levels in the serums of patients with primary ITP and secondary ITP. The samples were taken from Wahidin Sudirohusodo Hospital and the specimens were examined in the Research Unit Laboratory of the Faculty of Medicine, Universitas Hasanuddin/Hospital of Universitas Hasanuddin. The comparative test resulted in p-value = 0.005, where p <α = 0.05; and therefore, there was a significant difference between IL 17 levels in ITP and non-primary ITP
Metagenomic Diversity of Gut Microbiota of Gestational Diabetes Mellitus of Pregnant Women
Get PDFGestational Diabetes Mellitus (GDM) is defined as a condition in which a woman without diabetes develops abnormal glucose tolerance that is first recognized during pregnancy. GDM is a significant public health problem with an incidence of 1.9 – 3.6% of all pregnancies in Indonesia. Additionally, women with GDM during pregnancy have a high risk of developing diabetes when they are not pregnant, such as type 2 diabetes (T2D). One alternative variable in the management of T2D globally is gut microbiota. Here, to find out the role of gut microbiota in pregnancy, we characterized the stools of 30 pregnant women, each consisting of fifteen GDM-detected pregnant women, and healthy pregnant women using metagenomic approach with genome analysis by directly isolating genomic DNA from the microbiota ecosystem that occupies the digestive tract. DNA sequencing results were analyzed by MEGA 6 software with the BLASTn algorithm in NCBI. Thus fifteen GDM-detected showed high nucleotide sequence homology with the Proteobacteria at phylum level, and Escherichia, Orchobacterium, Cronobacter, Shigella, Salmonella, Enterobacter, Klebsiella, Kosakonia, Vibrio dan Gamma-Proteobacterium at genus level compared to the healthy pregnant women which found by Firmicutes at phylum level and Ruminococcus, Clostridium, Clostridiales, Lachnospiraceae, Roseburia, Weisella, Eubacterium at genus level had a higher abundance in healthy pregnant women. In this result, we found also one of the fifteen healthy pregnant women showed differential abundance with enrichment of Prevotella species. Gut microbiota of GDM-diagnosed pregnant women has more varied composition, and dominated by the phylum Proteobacteria than in normal pregnant women.Gestational Diabetes Mellitus (GDM) is defined as a condition in which a woman without diabetes develops abnormal glucose tolerance that is first recognized during pregnancy. GDM is a significant public health problem with an incidence of 1.9 – 3.6% of all pregnancies in Indonesia. Additionally, women with GDM during pregnancy have a high risk of developing diabetes when they are not pregnant, such as type 2 diabetes (T2D). One alternative variable in the management of T2D globally is gut microbiota. Here, to find out the role of gut microbiota in pregnancy, we characterized the stools of 30 pregnant women, each consisting of fifteen GDM-detected pregnant women, and healthy pregnant women using metagenomic approach with genome analysis by directly isolating genomic DNA from the microbiota ecosystem that occupies the digestive tract. DNA sequencing results were analyzed by MEGA 6 software with the BLASTn algorithm in NCBI. Thus fifteen GDM-detected showed high nucleotide sequence homology with the Proteobacteria at phylum level, and Escherichia, Orchobacterium, Cronobacter, Shigella, Salmonella, Enterobacter, Klebsiella, Kosakonia, Vibrio dan Gamma-Proteobacterium at genus level compared to the healthy pregnant women which found by Firmicutes at phylum level and Ruminococcus, Clostridium, Clostridiales, Lachnospiraceae, Roseburia, Weisella, Eubacterium at genus level had a higher abundance in healthy pregnant women. In this result, we found also one of the fifteen healthy pregnant women showed differential abundance with enrichment of Prevotella species. Gut microbiota of GDM-diagnosed pregnant women has more varied composition, and dominated by the phylum Proteobacteria than in normal pregnant women
Pengujian Resistensi Staphylococcus aureus Hasil Isolasi Udara Ruang Kelas Sekolah Dasar Di Surakarta terhadap Penisilin G, Metisilin dan Vankomisin
Get PDFStaphylococcus aureus is the bacteria that cause serious infections. This is supported by the presence of Staphylococcus aureus resistance to antibiotics. Air condition in the Elementary School classroom as a factor enabling the spread of infectious diseases in which elementary school is a community that consist of children aged between 6-12 years on average has not been able to be independent terms of cleanliness. Especially with irrational or excessive use of antibiotics in children appears to be more frequent and increasingly alarming. This research begins by sampling the air of bacteria with medium Vogel Johnson Agar to be opened for 30 minutes in elementary school classrooms. Samples obtained from nine elementary schools in Surakarta. Incubation at 37ºC for 24 hours. Identification test carried Staphylococcus aureus bacteria and then isolated. Staphylococcus aureus obtained is used as the test bacteria to antibiotics penicillin G, methicillin and vancomycin. The result of this study was from 27 point in nine elementary school there are 20 point containing Staphylococcus aureus. Based on sensitivity tests there are 30 % resistant to penicillin G, 5 % for methicillin and not anything for vancomycin. Mean while based on penicillinase test there are 6 point that Staphylococcus aureus produces penicillinase enzyme. Staphylococcus aureus merupakan bakteri penyebab infeksi yang dapat menyebabkan infeksi yang serius. Hal ini didukung oleh adanya resistensi Staphylococcus aureus terhadap antibiotik. Sekolah Dasar merupakan komunitas dari anak usia antara 6-12 tahun yang rata-rata belum mampu mandiri dalam kebersihan. Apalagi dengan penggunaan antibiotika irrasional atau berlebihan pada anak yang semakin meningkat dan mengkhawatirkan. Penelitian ini diawali dengan pengambilan sampel bakteri udara dengan medium Vogel Johnson Agar yang dibuka selama 30 menit di ruang kelas Sekolah Dasar. Sampel diperoleh dari sembilan Sekolah Dasar di Surakarta. Inkubasi pada suhu 37ºC selama 24 jam. Dilakukan uji identifikasi bakteri Staphylococcus aureus kemudian diisolasi. Bakteri Staphylococcus aureus yang diperoleh digunakan sebagai bakteri uji terhadap antibiotik penisilin G, metisilin dan vankomisin. Hasil yang diperoleh dari 27 titik lokasi pengambilan pada sembilan Sekolah Dasar tersebut terdapat 20 titik lokasi yang mengandung Staphylococcus aureus. Berdasarkan uji sensitivitas diperoleh hasil 30 % Staphylococccus aureus resisten penisilin G dan 5 % Staphylococccus aureus resisten metisilin, namun tidak ada yang resisten vankomisin. Pada uji penisilinase diketahui bahwa terdapat Staphylococcus aureus pada 6 titik lokasi pengambilan yang menghasilkan enzim penisilinase
RASA SYUKUR, DUKUNGAN SOSIAL, DAN KESEJAHTERAAN PSIKOLOGIS PURNA JABATAN PEGAWAI NEGERI SIPIL
Get PDFRetirement is a very complex lifestyle transition. If you aren’t ready to deal with these changes then feel unhappiness will most likely occur. Meeting the needs of psychological well-being is a form happiness. Gratitude is a form of happiness expressed by someone and is accosiated to psychological well-being. In addition to gratitude, social support can have a positive impact on individual’s helath and well-being. This studi aims to determine the relationship of gratitude and social support for psychological well-being in post-service civil servants. The subjects used in this study were full-time civil servants, totaling 80 men and women. In this study, Sampling using incidental sampling techniques. This study uses a quantitative approach. The data collection tools used in this study were gratitude scale, social support scale, and psychological well-being scale. Data analysis was performed with multiple regression tests. Based on the result of the analysis shows that there is a positive and significant relationship between gratitude and social support with psychological well-being in the post of civil service.Retirement is a very complex lifestyle transition. If you aren’t ready to deal with these changes then feel unhappiness will most likely occur. Meeting the needs of psychological well-being is a form happiness. Gratitude is a form of happiness expressed by someone and is accosiated to psychological well-being. In addition to gratitude, social support can have a positive impact on individual’s helath and well-being. This studi aims to determine the role of gratitude and social support for psychological well-being in post-service civil servants. The subjects used in this study were full-time civil servants, totaling 80 men and women. In this study, Sampling using incidental sampling techniques. This study uses a quantitative approach. The data collection tools used in this study were gratitude scale, social support scale, and psychological well-being scale. Data analysis was performed with multiple regression tests. Based on the result of the analysis shows that there is a positive and significant relationship between gratitude and social support with psychological well-being in the post of civil servic
APAKAH KINERJA KEUANGAN MEMEDIASI PENGARUH INTELLECTUAL CAPITAL TERHADAP REAKSI INVESTOR PADA PERUSAHAAN PERBANKAN DI INDONESIA (?)
Get PDFIntellectual capital is vital for companies to improve competitiveness. Company stakeholders are starting to realize that intellectual capital is one of the intangible assets that need to be managed properly because it helps companies compete. This study aims to examine financial performance in mediating the effect of intellectual capital on investor reaction. Financial performance is measured using a return on assets, intellectual capital as an independent variable is measured using value-added Intellectual capital (VAICTM) which consists of three components, namely: human capital, structural capital, and relational capital, while investor reactions are measured using abnormal returns. The object of research is the banking sector companies listed on the Indonesia Stock Exchange (IDX) in 2016-2018 by the determined purposive sampling. This study uses Structural Equation Modeling (SEM) for data analysis and hypothesis testing, and uses the WarpPLS version 7.0 software to process data. Based on the test results, several conclusions: (a) Intellectual has a positive and significant effect on investor reaction, (b) Intellectual capital has a positive and significant effect on financial performance, (c) financial performance has a positive and significant effect on investor reaction, and (d) financial performance can fully mediate the effect of Intellectual capital on investor reactions
Efektivitas dan Keamanan Terlipressin pada Acute Variceal Haemorhage: Sebuah Kajian Naratif
No full textProviding appropriate yet timely treatment to stop bleeding is the key pathway in the management of acute variceal haemorhage (AVH). Several agents can be used to manage bleeding, and one of the new agent recently approved in Indonesia in 2019 is Terlipressin. Information toward the efficacy dan safety of Terlipressin is essentially needed to help optimising effort to use this agent responsibly, specifically in this early phase of entering Indonesian market.
This narrative review mainly includes the basic profile, the current updated evidence of the efficacy and safety, the position in the therapy, and the cost of Terlipressin compared with other vasoactive agents. Only systematic review and meta-analysis articles were used to describe the efficacy and safety of Terlipressin considering it were ranked at the top level of evidence-based ladder.
Some unique pharmacokinetics and pharmacodynamics properties of Terlipressin, which are not found in other vasoactive, can provide advantages when it is administered to patients in the clinical practice. However, it does not necessarily mean that Terlipression is more superior compared to other vasoactive agents. Findings of our review on three systematic review and meta-analysis found that the efficacy and safety of Terlipressin were not significantly different compared to other vasoactive agents. Therefore, Terlipressin is not the only recommended agent to be used as the first-line vasoactive agent in the management of AVH. Owing to it is relatively new introduced in Indonesia, the cost of using Terlipressin is relatively higher compared to other first-line vasoactive agent. It therefore could be suggested to use Terlipressin only for patients who can attain benefit at most such as patients with contraindications or history of side effect from other vasoactive agentsPenanganan secara cepat dan tepat untuk menghentikan pendarahan merupakan salah satu pilar penting dalam tata laksana kasus acute variceal haemorhage (AVH). Terdapat beberapa pilihan obat yang dapat digunakan untuk menghentikan pendarahan yang terjadi, salah satunya dengan menggunakan Terlipressin, sebuah obat yang baru masuk Indonesia pada tahun 2019. Pengetahuan terkait efektivitas dan keamanan penggunaan Terlipressin sangat diperlukan untuk menunjang penggunaan obat yang optimal dan mencegah penggunaan obat secara berlebihan khususnya pada periode awal keberadaan obat tersebut di Indonesia.
Kajian pustaka ini bersifat naratif dan memaparkan beberapa data terkait: profil dasar Terlipressin, efektivitas dan keamanan penggunaan pada kasus AVH yang berbasis bukti (evidence-based), posisi terapi menggunakan Terlipressin pada beberapa pedoman terapi, serta komparasi biaya terapi Terlipressin bila dibandingkan obat vasoaktif lainnya. Hanya pustaka terpublikasi dalam bentuk kajian sistematis dan meta-analisis yang digunakan dalam tinjauan efektivitas dan keamanan.
Terlipressin memiliki beberapa karakteristik farmakokinetik dan farmakodinamik yang dapat memberikan keuntungan terkait teknis pemberian dibandingkan dengan beberapa obat vasoaktif yang lain. Namun demikian, hal tersebut tidak berarti bahwa Terlipressin lebih unggul dibandingkan dengan jenis vasoaktif yang lain. Hasil kajian terhadap tiga (3) artikel kajian sistematis dan meta-analisis menunjukkan bahwa efektivitas dan keamanan Terlipressin tidak lebih baik secara signifikan dibandingkan dengan obat vasoaktif lain. Oleh karena itu, Terlipressin bukan merupakan satu-satunya obat yang direkomendasikan sebagai pilihan pertama vasoaktif pada tata-laksana AVH. Sebagai obat yang relatif baru, biaya penggunaan Terlipressin lebih tinggi dibandingkan vasoaktif pilihan pertama yang lain. Dengan mempertimbangkan beberapa hal tersebut, Terlipressin sebaiknya digunakan pada pasien yang dapat memperoleh keuntungan klinis optimal, misalnya pasien dengan kontraindikasi atau riwayat efek samping dengan vasoaktif yang lain
PERBEDAAN PERSEPSI COMPANIONSHIP DALAM PERNIKAHAN ANTARA LAKI-LAKI DAN PEREMPUAN
Get PDFIndonesian’s divorce rate is the highest among countries in Asia-Pacific, with 70% from total cases were submitted by women. Based on BKKBN data, there are 200 thousands divorce cases each year, and over two millions marriages each year. Highest causes of these divorce cases are marital-abuse (physically and emotionally) and infidelity.These causes indicate about failure in maintaining companionship between husband and wife. This research aims to find out whether there are differences of companionship in marriage between men and women. Data were collected online. There were 105 total subjects, 54 men and 51 women. Hypothesis analysis were done with Independent t-test method. Results of this research shown that there is significance differences in companionship between men and women (sig=0,014
The Effect of Seaweed Combination on the Extract of Robusta Coffee (Coffea robusta) Waste Extract in Producing Facial Mask Products
Get PDFAceh is one of the biggest coffee producers in Indonesia’s province. The coffee farmers separate the coffee beans with the flesh of the fruit for processing the seeds, while the coffee fruit is considered as waste used as animal feed. Based on existing research, the coffee flesh produced contains polyphenol compounds as antibacterial. A very promising sea product from Aceh is seaweed. Brown seaweed (Sargassum sp.) is very common in the west coast of Aceh province. One of the derivatives is alginate. In this study, a combination of polyphenols extracts from coffee flesh and alginates from seaweed Sargassum sp. used as a basic ingredient in making face masks. Tests on organoleptic observations, pH testing, testing time of dried preparations, and testing of bacterial activity have been carried out in this study. In addition, variations in the mixture preparation based on the particle size of alginate powder have also been carried out in this study. The results obtained indicate that the yield produced from coffee fruit extracts is 5.86%, the highest yield of alginate is by potassium carbonate of 40.77%, the acidity of the combination is 4.5 - 6.5. For preparations, drying time has an average estimated time of approximately 6 minutes. Bacterial activity test showed that K2CO3 extracting agent concentration of 2% was strong against bacterial growth with a clean area of 14 mm. With these results, it can be seen the combination is able to inhibit the rate of bacterial growth, so it is recommended for alternative ingredients in the manufacture of cosmetics pharmaceutical industry masks