Universitas Setia Budi Surakarta: USB e-journal
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The Potential of Iodine Lactoglycerol as an Alternative Fungal Stain in the Laboratory
Get PDFFungi are eukaryotic and opportunistic organisms that can cause damage and disease. Fungi can be identified by direct staining. The composition of the dyes used in fungi staining are toxic, mutagenic and harmful to laboratory personnel and the environment. Consequently, it is necessary to consider use fungi dye continuously, so its need alternative safety dyes to identify fungi in the laboratory. This research aims to determine the potential of iodine lactoglycerol with different concentrations (1.25%, 2.5%, and 5%) as an alternative dye for fungi in the laboratory. The research method used experimental design. The samples originate from moldy bread and made 27 of microscopic slides. The microscopic slides were observed with objective 40x magnification. The parameters assessed in this study were the structure and morphology of fungi including hyphae, conidiophores, vesicles, sterigmata and conidiospores and color intensity using the image analysis of ImageJ. The results showed that various concentrations of iodine lactoglycerol can staining the structure and morphology of the fungi properly and clearly. The value of color intensity of each concentration measured has a different average. The highest average value of color intensity concentration was 5% and the lowest was 1.25%. However, the result of ANOVA test showed a significance of 0.380 (P> 0.05), which means that is no difference significantly from three of various concentrations. The result showed that the difference concentration of iodine lactoglycerol does not affect the color intensity. The conclusion of this study was iodine lactoglycerol solution can be used as an alternative dye or Lactophenol-cotton Blue (LPCB) substitute for fungi identification. It is recommendation for further research be carried out regarding the decrease in the concentration of alternative color solutions below 1.25%, the incubation time for the preparation of the preparations, and the color stability of the prepared mushroom preparations
TEKNIK RELAKSASI OTOT PROGRESIF UNTUK MENGURANGI STRES PASCA TRAUMA PADA PENYINTAS GEMPA PALU YANG DI UNGSIKAN DI KABUPATEN MALANG
Get PDFThe magnitude 7.7 earthquake that struck Palu on September 28, 2018, certainly had a psychological impact on those who experienced the disaster. As a result of traumatic events experienced by survivors require special psychological treatment. The impact experienced is ongoing stress followed by a traumatic experience. This study aims to reduce posttraumatic stress in survivors through progressive muscle relaxation therapy. The population in this study were 50 survivors of hammer earthquake in Malang Regency. The sampling technique used was purposive sampling with samples obtained as many as 3 people who indicated pathological symptoms. Data collection uses observation, interviews and documentation. While the interventions used in this study were Progressive Muscle Relaxation Therapy which consisted of 3 sessions in 1 meeting for 60 minutes and held 4 meetings a week. Session 1 identified the perceived muscle tension, session 2 carried out progressive muscle relaxation therapy, and session 3 distinguishes and compares what is felt when muscles are stretched and relaxed. This progressive muscle relaxation technique aims to reduce stress due to traumatic experiences. The results showed that this progressive muscle relaxation technique can reduce stress resulting in muscle tension such as muscle cramps, headaches, anxiety, insomnia and excessive stress because of the trauma experienced by the three survivors. Progressive muscle relaxation performed has been shown to reduce the physiological and emotional components of stress experienced by the three survivors so that the three survivors have decreased stress.Peristiwa gempa berkekuatan 7,7 SR yang mengguncang Palu pada tanggal 28 September 2018, tentunya memberikan dampak psikologis pada mereka yang mengalami bencana. Di Kabupaten Malang ada 50 orang penyintas gempa palu yang diungsikan. Ditinjau dari kondisi psikologis terdapat 3 penyintas yang terindikasi gejala patologis akibat kejadian traumatik yang dialami dan membutuhkan penanganan khusus secara psikologis yaitu stres pasca trauma atau Post Traumatic Stress (PTS) merupakan stres yang berlangsung mengikuti kejadian traumatis. Penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian studi kasus. Pengumpulan data menggunakan observasi, wawancara dan dokumentasi. Sedangkan Intervensi yang digunakan dalam penelitian ini adalah Terapi Relaksasi Otot Progresif yang terdiri dari 3 sesi dalam 1 kali pertemuan selama 60 menit dan dilakukan sebanyak 4 kali pertemuan dalam seminggu. Pada sesi 1 yaitu mengidentifikasi ketegangan otot yang dirasakan, sesi 2 yaitu pelaksanaan terapi relaksasi otot progresif, dan sesi 3 membedakan perasaan yang dialami dan membandingkan ketika otot-otot ditegangkan dan dilemaskan.. Teknik relaksasi otot progresif ini bertujuan untuk mengurangi stres pasca trauma pada penyintas. Hasil penelitian menunjukkan teknik relaksasi otot progresif ini dapat mengurangi stres yang berakibat ketegangan otot seperti keram otot, otok kaku, sakit kepala dan lain-lain dalam kondisi kecemasan, sulit tidur dan stres yang berlebihan karna pasca trauma bencana yang dialami ketiga penyintas. Setelah dilakukan relaksasi otot progresif diketahui bahwa ketiga penyintas mengalami penurunan terhadap stres. Relaksasi otot progresif yang dilakukan dengan atau tanpa ketagangan otot dan teknik manipulasi pikiran terbukti dapat mengurangi komponen fisiologis dan emosional stres yang dialami oleh ketiga penyintas.
Kata Kunci: Relaksasi Otot Progresif, Stres Pasca Trauma, Penyinta
Optimasi Pelarut Ekstraksi, Aktivitas Anthelmintika terhadap Ascaris lumbricoides Suis., dan Telaah Fitokimia Bunga Ekor Kucing (Acalypha hispida Burm. F.)
Get PDFRed hot cat's tail (Acalypha hispida Burm. F.) flowers is a traditional medicinal plant which can be used as an antelmintic. The aim of present study was to determine the solvent extraction of Red hot cat's tail flowers that produce the highest yield, to determine antelmintic activity against roundworms (Ascaris lumbricoides Suis.) and to determine the compounds in the extract. The powder was extracted by maceration with ethanol with four variations concentration. Extract was analyzed the soluble in ethanol extract concentration and the yield. The highest yield extract was tested on roundworms by in vitro. The worms were grouped into 12 groups randomly without regard to gender. The group consists of one negative control group was be given distilled water, the positive control group was be given piperazine citrate in 5 different concentrations, and the red hot cat's tail flowers group with 6 variations of concentration. Observation of the worm is done after 24 hours of treatment. Data were analyzed by probit method to determine the LC50. The results of this study were solvent extraction that produces the highest yield was 70% ethanol. The 70% ethanol extract had an antelmintic activity against roundworms with 6,61% w/v as LC50 values that equivalent to 0,12% w/v piperazine citrate. The 70% ethanol extract contained saponins, flavonoids, and tannins.Bunga ekor kucing (Acalypha hispida Burm. F.) merupakan tanaman obat tradisional yang dapat digunakan sebagai obat cacing. Tujuan penelitian ini adalah mengetahui pelarut ekstraksi bunga ekor kucing yang menghasilkan randemen total terbanyak, mengetahui efek anthelmintiknya terhadap cacing gelang (Ascaris lumbricoides Suis.) dan mengetahui golongan senyawa dalam ekstraknya. Serbuk simplisia diekstraksi secara maserasi dengan etanol dengan empat variasi konsentrasi. Ekstrak dianalisis kadar sari terlarut dalam etanol dan randemen totalnya. Ekstrak bunga ekor kucing dengan rendemen terbanyak diujikan pada cacing gelang secara in vitro. Cacing dikelompokkan menjadi 12 kelompok yang dipilih secara acak tanpa memperhatikan jenis kelamin. Kelompok tersebut terdiri dari 1 kelompok kontrol negatif yaitu aquadest, kelompok kontrol positif yaitu piperazin sitrat dalam 5 konsentrasi yang berbeda, dan kelompok ekstrak bunga ekor kucing dengan 6 variasi konsentrasi. Pengamatan terhadap cacing dilakukan setelah 24 jam perlakuan bahan uji. Data dianalisa dengan metode probit untuk mengetahui LC50. Hasil dari penelitian ini adalah pelarut ekstraksi yang menghasilkan randemen total ekstrak bunga ekor kucing terbanyak adalah etanol 70%. Ekstrak etanol 70% itu mempunyai efek anthelmintik terhadap cacing gelang dengan nilai LC50 sebesar 6,61% b/v yang setara dengan 0,12% b/v piperazin sitrat. Ekstrak etanol 70% bunga ekor kucing mengandung saponin, flavonoid, dan tanin
Pengaruh Pemberian Jus Asam Jawa Secara Oral terhadap Profil Farmakokinetika Asetosal pada Kelinci Jantan New Zealand White
Get PDFAcetosal is the oldest of pain medication which till now the most widely used worldwide and very effective to relieve pain with mild to moderate intensity, but so far the public lack an understanding of the role of food and beverages included when taking the drug. Tamarind juice is a beverage that contains acid, which acetosal also has acidic properties. If acetosal consumed together with the tamarind juice, it will cause gastric pH increases so the absorption of acetosal is increasing. The experiment was aimed to know the influence of tamarind juice given orally on acetosal pharmacokinetic profile in New Zealand White male rabbits. This experiment was divided into two groups i.e. control group and treatment group. The control group was given acetosal of 70 mg/1.5kg BW of rabbit, and the treatment group was given acetosal of 70 mg/1.5kg BW of rabbit and tamarind juice of 17.5 ml/1.5kgBW. Blood was drawn through marginal vena with sampling time at minute 5, 10, 15, 20, 30, 40, 50, 60, 120, 180, 240, 300 and 360. Determination of total salisylic acid level in blood was done by UV-Vis spectrophotometer according to modified different method. The result of the experiment showed that tamarind juice affected the pharmacokinetic profile of acetosal indicated by value increased of Ka, ClT, Vc, Vdss, and t½ β and value decreased of t max, K21, K, K12, AUC, α, and βAsetosal merupakan obat nyeri tertua, yang sampai saat ini paling banyak digunakan di seluruh dunia dan sangat efektif meredakan nyeri dengan intensitas ringan sampai sedang, namun sejauh ini masyarakat kurang memahami akan peran makanan maupun minuman yang disertakan saat mengkonsumsi obat. Jus asam jawa merupakan minuman yang mengandung asam, yang mana asetosal juga memiliki sifat asam. Asetosal apabila dikonsumsi bersamaan dengan jus asam jawa akan menyebabkan pH lambung meningkat sehingga absorbsi asetosal meningkat. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian jus asam jawa secara oral terhadap profil farmakokinetila asetosal pada kelinci jantan New Zealand White. Penelitian ini dibagi menjadi dua kelompok yaitu kelompok kontrol dan kelompok perlakuan. Kelompok kontrol diberikan asetosal 70 mg/1,5 kg BB kelinci dan kelompok perlakuan diberikan asetosal 70 mg/1,5 kg BB dan jus asam jawa 17,5 ml/1,5 kg BB. Darah diambil melalui vena marginalis dengan waktu dari menit ke-5, 10, 15, 20, 30, 40, 50, 60, 120, 180, 240, 300, dan 360. Penetapan kadar asam salisilat dalam darah ditetapkan dengan spektrofotometer UV-Vis menurut metode different yang dimodifikasi. Hasil penelitian menunjukkan bahwa jus asam jawa mempengaruhi profil farmakokinetika asetosal berupa kenaikan besaran Ka, Vc, Vdss, ClT dan t½ β dan penurunan besaran t max, K21, K, K12, AUC, α, and β
KEPERCAYAAN PENGGUNAAN SISTEM RESEP ONLINE PADA TENAGA KESEHATAN DI SURAKARTA
Get PDFThis study aims to test the trust caused by technological knowledge, compatibility, and system security. Previous studies have not yet explained the role of trust in the relationship between technological knowledge, compatibility, and security in the intention to continue the online prescription system. Data was obtained through an online questionnaire that was distributed to health professionals using the Surakarta online prescription system. The sampling technique used was purposive sampling with a population of 200 health workers. Hypothesis testing is done by using SEM. The results of this study indicate that technology and security knowledge have a role in increasing trust to form intentions to use online prescription systems. There are hypotheses that are not supported by compatibility which is felt to have no role in increasing trust to form intentions using an online prescription system.This study aims to test the trust caused by technological knowledge, compatibility, and system security. Previous studies have not yet explained the role of trust in the relationship between technological knowledge, compatibility, and security in the intention to continue the online prescription system. Data was obtained through an online questionnaire that was distributed to health professionals using the Surakarta online prescription system. The sampling technique used was purposive sampling with a population of 200 health workers. Hypothesis testing is done by using SEM. The results of this study indicate that technology and security knowledge have a role in increasing trust to form intentions to use online prescription systems. There are hypotheses that are not supported by compatibility which is felt to have no role in increasing trust to form intentions using an online prescription system
The Screening of Antioxidant Activities of Meniran Plants (Phyllanthus niruri Linn) and Guava Leaves (Psidium guajava Linn) Combination Using DPPH Free Radical Method
Get PDFDegenerative disease is a serious health problem and causes a lot of deaths in Indonesia. One of the most dangerous degenerative diseases is cancer. Free radicals play a role in oxidative stress in the later stages of carcinogenesis. Antioxidant delays or inhibits cellular damage mainly through its free radical scavenging property. Meniran plants and guava leaves have high radical-scavenging activities. The previous studies reported that the combination of ginger and Meniran plant extract has a stronger antioxidant activity than the extract of a single plant. This research aims at determining the antioxidant activities of Meniran plants and guava leaves compared with its singular form. This research was conducted from October to December 2016 at the Chemical Laboratory of Nasional Health Science Institute and the Center for Development and Research of Medicinal Plants and Traditional Medicine, Tawangmangu, Karanganyar. The antioxidant activity assay was done using DPPH free radical method and vitamin C was used as the control. They were measured with UV-Vis Spectrophotometer. This study concludes that the IC50 value of Meniran plants was 30.689 ppm and the IC50 value of guava leaves was 13.7859. The IC50 values of Meniran plant and guava leaf combination with various ratios were 20.6095 ppm (1:1), 12.5629 ppm (1:2), and 16.841 ppm (2:1). The combination of Meniran plant and guava leaf extract (1: 2) had the strongest antioxidant activity of 12.56 ppm
Pengaruh Pemberian Ekstrak Adas (Foeniculum vulgare Mill ) Pada Tikus Laktasi Terhadap Sel Neuroglia Anak Tikus
Get PDFBreast Milk is a natural baby food that is rich in nutrients and contains higher immunological factors compared to formula milk. Foeniculum vulgare Mill plants have long been used by the community as enhancing the production of breast milk (ASI). The purpose of this study was to determine the effect of lactogogum extracts of Foeniculum vulgare Mill leaves and stems in lactation mice, as seen from mouse neuroglia cells.
Foeniculum vulgare Mill extract was made by maceration method using 96% ethanol solvent. The test animals used in the study were white rats, divided into 6 groups namely, normal control, control without drugs (cmc-na 0.5%), comparison of asifit drugs (67.86 mg / KgBB), ethanol extract groups of leaves and stems fennel dosage 315 mg / kg body weight, 630 mg / kg body weight, and 945 mg / kg body weight.
The results of the research that have been done show that the ethanol extract of Foeniculum vulgare Mill leaves and stems dose 315 mg / kg body weight, 630 mg / kg body weight, and 945 mg / kg body weight have a lactagogum effect seen from the parameters of the number of glia cells in the brains of breastfed mice. The effect increase is not as large as the dose increase given. The ethanol extract of Adas leaves and stems of 630 mg / kg BW has an optimal lactagogum effect equivalent to drug control on the market as seen from the parameters of the number of glia cells in the brains of breastfed rat.Air Susu Ibu (ASI) merupakan makanan bayi alamiah yang kaya akan nutrisi dan mengandung faktor imunologis yang lebih tinggi dibanding dengan susu formula. Tanaman adas telah lama digunakan oleh masyarakat sebagai peningkat produksi air susu ibu (ASI). Tujuan dari penelitian ini adalah untuk mengetahui efek laktagogue ekstrak daun dan batang adas pada tikus laktasi, dilihat dari sel neuroglia anak tikus.
Ekstrak adas dibuat dengan metode maserasi menggunakan pelarut etanol 96%. Hewan uji yang digunakan adalam penelitian adalah tikus putih, dibagi menjadi 6 kelompok yaitu, kontrol normal, kontrol tanpa obat (cmc-na 0,5%), pembanding obat asifit (67,86 mg/KgBB), kelompok ekstrak etanol daun dan batang adas dosis 315 mg/kg BB, 630 mg/kg BB, dan 945 mg/Kg BB.
Hasil penelitian yang telah dilakukan menunjukkan bahwa ekstrak etanol daun dan batang adas dosis 315 mg/kg BB, 630 mg/kg BB, dan 945 mg/Kg BB mempunyai efek laktagogum dilihat dari parameter junlah sel glia pada otak anak tikus yang disusui. Kenaikan efek tidak sebesar kenaikan dosis ang diberikan. Dosis ekstrak etanol daun dan batang adas dosis 630 mg/kg BB mempunyai efek laktagogue yang optimal setara dengan kontrol obat di pasaran dilihat dari parameter jumlah sel glia pada otak anak tikus yang disusui
The Effect Cytotoxic and Apoptotic Propagation Chloroform Fraction of Ocimum sanctum L. Towards Hela Cells Line Culture
Get PDFMaterials used in the research are Ocimum sanctum L, a herbaceous shrub used in traditional medicine displaying as immunomodulator, anti-stress, hepatoprotective, kemopreventif, and anti-inflammatory. A research to identify its ethanol extract of Ocimum sanctum has been conducted, showing that it possesses cytotoxic activities to HeLa cells with IC50 value of 209µg/ml. The aim of this research is to find out activities cytotoxic and apoptotic propagation chloroform fraction of Ocimum sanctum towards HeLa cells line. The extraction was done using maceration using ethanol 96% solvent and fractionation was done using chloroform. Effects of chloroform fraction on HeLa cells were tested using MTT at various dosages (500, 250, 125, 62.5, and 31.25) µg/ml. Cisplatin (Kalbe) was applied as positive control. The effect apoptotic propagation chloroform fraction of Ocimum sanctum on HeLa cells was observed by DNA painting method using orange ethridium bromide-acridine. The test results showed that the chloroform fraction of Ocimum sanctum extract on HeLa cells had an IC50 value of 155.67 µg / ml ± 46.99 and Cisplatin had an IC50 value of 16.81 µg / ml ± 20.37. Double staining test shows the existence of cells undergoing apoptosis. Based on these results, the chloroform fraction Ocimum sanctum has been shown to be cytotoxic and has the possibility of stimulating apoptosis of cervical cancer cells (HeLa). This can be used as a basis for the development of these plants as anticancer agents with specific action targets
DINAMIKA SELF-FORGIVENESS MANTAN PECANDU NARKOBA DI YAYASAN MITRA ALAM SURAKARTA
Get PDFABSTRAK
Tujuan penelitian ini adalah untuk menemukan faktor-faktor yang mendorong serta memberikan gambaran dinamika mantan pecandu narkoba di Yayasan Mitra Alam Surakarta untuk melakukan self forgiveness.Informan dalam penelitian ini adalah mantan pecandu narkoba yang merupakan warga binaan dari Yayasan Mitra Alam yang memiliki rasa bersalah terhadap diri sendiri terhadap perilakunya di masa lalu dan saat ini sedang melakukan proses self forgiveness. Metode penelitian dalam penelitian ini menggunakan pendekatan kualitatif deskriptif. Metode pengumpulan data yang dilakukan adalah melalui observasi dan wawancara. Teknik analisis data menggunakan model interaktif Miles & Huberman Hasil penelitian : faktor-faktor yang mempengaruhi pemaafan diri (self forgiveness) kedua informan ada beberapa yang sama yaitu : suka bersosialisasi, mau memaafkan pelaku karena merasa bahwa apa yang informan alami adalah kesalahan mereka bukan pelaku,atribusi terhadap pelaku dimana apa yang sudah dilakukan oleh pelaku didasari oleh motif ekonomi namun perbedaannya subyek kedua juga melihat bahwa pelaku melakukan hal tersebut karena didasari oleh motif keakraban dalam pertemanan. ia mencoba untuk berpikir di sisi lain (reframing) bahwa peristiwa yang terjadi di masa lalunya juga dialami oleh orang lain, bukan hanya dirinya saja. Secara umum, dinamika dari proses pemaafan diri kedua informan yang berawal dari sebuah kejadian penyalahgunaan narkoba hingga akhirnya muncul rasa bersalah dan karena merasa tidak nyaman dengan hal tersebut, kedua informan melakukan tahap pemaafan diri yang didukung oleh faktor-faktor diatas. Kedua informan tersebut mereka sama-sama sudah berusaha untuk melakukan tahapan dalam memaafkan diri (self forgiveness), namun ada perbedaan dalam pencapaian tahap pemaafan
PERAN MEDIASI RENCANA BEROBAT DAN MODERASI KONTROL KEPERILAKUAN AKTUAL DAN KONTEKS SITUASIONAL DALAM HUBUNGAN ANTARA NIAT BEROBAT
Get PDFThis study aims to examine the mediating role of the implementation plan in the relationship between medical intention and treatment decisions, examine the role of moderation of actual behavioral control in the relationship between implementation plans and treatment decisions, and examine the role of situational context moderation in the relationship between treatment plans and treatment decisions. The decision to treat a patient is important for the hospital because it determines the number of patients who will seek treatment at the hospital. Data obtained through questionnaires distributed to patient or someone who want to be a patient. The research sample used amounted 200. This research use purposive sampling in Solo Raya. Hypothesis testing used regression logistic for mediating and moderating role. First hypothesis are supported but second and third hypothesis was not supported