Universitas Setia Budi Surakarta: USB e-journal
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Performance Test of Homemade Control Materials from Addition and Spike Placebo Simulation on Urine Chloride Examination with Fantus Method
Get PDFThe background of this research was to examine the performance of homemade urine control materials on urine chloride examination. This study was aimed to test the performance of homemade control materials from addition and spike placebo simulation on urine chloride examination using the Fantus method. This research applied an experimental research method. The stages of the study started with standardization of AgNO3 solution, preparation of urine control materials from addition and spike placebo simulation, as well as calculation of accuracy (%R), bias (%d) and coefficient of variation (%CV). The results showed that the normal and pathological levels of accuracy in the urine control materials from addition were 109.55% and 104.30%, while the accuracy values (%R) for urine control materials from spike placebo simulation, for the normal and pathological levels, were high, 110.07% and 104.54%. The bias values (%d) obtained in the urine control materials from addition with normal and high pathological levels were 9.55% and 4.30% bias, while the bias values (%d) in the urine control materials from spike placebo simulation with normal and high pathological levels were 10.07% and 4.54%. The coefficient variation (%CV) values in urine control materials from addition at normal and pathological high levels were 3.75% and 3.07%, while the %CV values in urine control materials from spike placebo simulation at normal and high pathological levels were 4.55% and 3.11%. This study concludes that based on the accuracy (%R) and bias (%d) parameters, the homemade control materials from addition and spike placebo simulation have relatively good performance for urine chloride examination using the Fantus method. Meanwhile, based on the parameter of coefficient of variation (%CVV), the homemade control materials from the addition and spike placebo simulation have slightly lower performance for examining urine chloride with the Fantus method.The background of this research was to examine the performance of homemade urine control materials on urine chloride examination. This study was aimed to test the performance of homemade control materials from addition and spike placebo simulation on urine chloride examination using the Fantus method. This research applied an experimental research method. The stages of the study started with standardization of AgNO3 solution, preparation of urine control materials from addition and spike placebo simulation, as well as calculation of accuracy (%R), bias (%d) and coefficient of variation (%CV). The results showed that the normal and pathological levels of accuracy in the urine control materials from addition were 109.55% and 104.30%, while the accuracy values (%R) for urine control materials from spike placebo simulation, for the normal and pathological levels, were high, 110.07% and 104.54%. The bias values (%d) obtained in the urine control materials from addition with normal and high pathological levels were 9.55% and 4.30% bias, while the bias values (%d) in the urine control materials from spike placebo simulation with normal and high pathological levels were 10.07% and 4.54%. The coefficient variation (%CV) values in urine control materials from addition at normal and pathological high levels were 3.75% and 3.07%, while the %CV values in urine control materials from spike placebo simulation at normal and high pathological levels were 4.55% and 3.11%. This study concludes that based on the accuracy (%R) and bias (%d) parameters, the homemade control materials from addition and spike placebo simulation have relatively good performance for urine chloride examination using the Fantus method. Meanwhile, based on the parameter of coefficient of variation (%CVV), the homemade control materials from the addition and spike placebo simulation have slightly lower performance for examining urine chloride with the Fantus method
Promosi Kesehatan Edukasi Penerapan Perilaku Hidup Bersih dan Sehat (PHBS) pada Pedagang di Pasar Tradisional
No full textSalah satu pencegahan yang dapat dilakukan untuk memutus rantai penularan Covid-19, yaitu dengan menerapkan perilaku hidup bersih dan sehat (PHBS). Masalah yang dihadapi di masyarakat adalah kurangnya pengetahuan dan kesadaran masyarakat mengenai pentingnya Perilaku Hidup Bersih dan Sehat (PHBS) selama pandemi Covid-19. Sasaran kegiatan pengabdian kepada masyarakat ini adalah masyarakat yang sering berinteraksi dengan orang banyak namun bukan tenaga medis (pedagang) di beberapa daerah di Surakarta, Kudus, Yogyakarta, dan Jambi. Tahapan kegiatan pengabdian meliputi identifikasi kondisi dan kebutuhan masyarakat, promosi kesehatan, dan evaluasi hasil promosi kesehatan melalui observasi dan wawancara. Kegiatan pengabdian kepada masyarakat berupa edukasi PHBS selama pandemi Covid-19 terhadap pedagang di empat kota (Surakarta, Yogyakarta, Kudus dan Jambi) telah berhasil mencapai target, yaitu perbaikan perilaku menggunakan masker, mencuci tangan, dan jaga jarak (social distancing) antar pembeli serta antara pedagang dengan pembeli. Masyarakat sasaran memberikan respon yang sangat baik dan bersedia menyediakan fasilitas tempat cuci tangan
Analysis of Chemical Components and Antibacterial Activity in Essential Oil of Lantana Flowers (Lantana Camara L)
Get PDFThe purpose of this study was to evaluate the essential oil composition as well as antibacterial activities of essential oil of Lantana camara L flowers against four bacterial strains. Essential oil of Lantana camara L flowers was obtained by hydrodistillation method using Sthal and analyzed by GC-MS. The antibacterial activities of essential oil were tested by using disk diffusion method against four bacterial strains. Results showed 72 compounds for Lantana flowers essential oil, of which caryophyllene (10,87%), davanone (9.84%), a-humulene (7.59%), a-curcumene (3.35%), germacrene D (3.09%), calarene (2.42%), a-muurolene (2.27%), p-cymene (1.79%), 1,8 cineole (1.59%) , δ-cadinene (1.59%), a-copaene (1.12%), nerolidol B (1%) and ß-ocimene (0.54%) were detected as major components. Antibacterial activity assay of Lantana camara L flowers essential oil was examined against four bacterial strains including Gram (+and-) by using disk diffusion agar method. All the tested Gram (+and-) bacterial strains displayed slight to moderate antibacterial activity (7 – 10 mm) against all concentrations of essential oil. The essential oil of Lantana camara L flowers showed remarkable antibacterial activity with inhibition in concentrations of 10% and 20% against the phatogen bacterias Basillus subtilis (9.6 mm; 10.8 mm), Propionibacterium acnes ( 7.6 mm; 8.7 mm), Eschechia coli ( 8.4 mm ; 9.6 mm) and Pseudomonas aeruginosa ( 8.3 mm ; 9.3 mm) showed from zone of inhibition that was formed, zone of inhibition in concentration 20% was bigger than concentration 10%.The purpose of this study was to evaluate the essential oil composition as well as antibacterial activities of essential oil of Lantana camara L flowers against four bacterial strains. Essential oil of Lantana camara L flowers was obtained by hydrodistillation method using Sthal and analyzed by GC-MS. The antibacterial activities of essential oil were tested by using disk diffusion method against four bacterial strains. Results showed 72 compounds for Lantana flowers essential oil, of which caryophyllene (10,87%), davanone (9.84%), a-humulene (7.59%), a-curcumene (3.35%), germacrene D (3.09%), calarene (2.42%), a-muurolene (2.27%), p-cymene (1.79%), 1,8 cineole (1.59%) , δ-cadinene (1.59%), a-copaene (1.12%), nerolidol B (1%) and ß-ocimene (0.54%) were detected as major components. Antibacterial activity assay of Lantana camara L flowers essential oil was examined against four bacterial strains including Gram (+and-) by using disk diffusion agar method. All the tested Gram (+and-) bacterial strains displayed slight to moderate antibacterial activity (7 – 10 mm) against all concentrations of essential oil. The essential oil of Lantana camara L flowers showed remarkable antibacterial activity with inhibition in concentrations of 10% and 20% against the phatogen bacterias Basillus subtilis (9.6 mm; 10.8 mm), Propionibacterium acnes ( 7.6 mm; 8.7 mm), Eschechia coli ( 8.4 mm ; 9.6 mm) and Pseudomonas aeruginosa ( 8.3 mm ; 9.3 mm) showed from zone of inhibition that was formed, zone of inhibition in concentration 20% was bigger than concentration 10%
KORELASI ADVERSITY QUOTIENT DENGAN PRESTASI NILAI SKOR TOSM PADA PENGURUS OSIS SMK
Get PDFThis study aims to empirically test the influence of adversity quotient on the achievement of ToSM (test of second mathematics) scores on the student. The hypothesis proposed is the influence of adversity quotient on the accomplishment of ToSM scores on students. The subjects in this study amounted to 34 students from SMK Negeri 1 Dukuhturi, Tegal Regency. The sampling technique used is saturated sampling. The measuring instruments in this study were adversity quotient scales and Android app-based ToSM. The results of this study showed the significance of the correlation test was 0.671 > 0.05 and Pearson correlation = 0.075 < r table = 0.3246, while the linearity test explained the magnitude of the correlation value / R relationship of 0.75 of the output obtained a coefficient of determination (R Square) of 0.006, which contains the understanding that the influence of Adversity Qutotien variable on variable performance ToSM score value is 0.6%, the data can be interpreted that there is no correlation between the variables studied, these findings can also be interpreted that to increase KHDI is not enough just by relying on adversity intelligence alone need other efforts.Penelitian ini bertujuan untuk menguji secara empiris pengaruh adversity quotient terhadap prestasi skor ToSM (test of second mathematics) pada siswa pengurus OSIS. Hipotesis yang diajukan adalah adanya pengaruh adversity quotient terhadap prestasi skor ToSM pada siswa pengurus OSIS. Subjek dalam penelitian ini berjumlah 35 siswa pengurus OSIS dari SMK Negeri 1 Dukuhturi, Kabupaten Tegal. Teknik pengambilan sampel yang digunakan adalah total sampling atau sampel jenuh. Alat ukur dalam penelitian ini adalah skala adversity quotient dan ToSM berbasis aplikasi Android. Analisis data dilakukan dengan menggunakan teknik analisis regresi sederhana dan hasil uji korelasinya adalah 0.671 > 0.05 dan pearson correlation = 0.075 < r table = 0.3246, adapun uji linearitas menjelaskan besarnya nilai korelasi / hubungan R yaitu sebesar 0.75 dari output tersebut diperoleh koefisien determinasi (R Square) sebesar 0.006, yang mengandung pengertian bahwa pengaruh varibel Adversity Qutotien terhadap variabel prestasi nilai skor ToSM adalah sebesar 0.6 % , data tersebut bisa dimaknai bahwa tidak ada korelasi antara variabel yang diteliti, temua ini dapat dimaknai juga bahwa untuk meningkatkan kompetensi hitung dasar intuitif (KHDI) tidak cukup hanya dengan mengandalkan kecerdasan adversity dan motivasi internal saja perlu adanya upaya lain yaitu latihan dan pembiasaan sedini mungkin
Potensi Krim Ekstrak Etanol Daun Stroberi (Fragaria x ananassa var Duchesne) sebagai Tabir Surya
Get PDFEkstrak daun stroberi dilaporkan memiliki aktivitas tabir surya yang baik. Formulasi sediaan yang baik menjadi hal yang diperlukan untuk tahapan berikutnya. Penelitian ini bertujuan untuk mengetahui mutu fisik dari sediaan krim dan menentukan nilai SPF krim ekstrak etanol daun stroberi (Fragaria x ananassa var duchesne) sebagai tabir surya.
Metode ekstraksi yang digunakan adalah refluks dengan pelarut etanol 96%. Analisis kandungan ekstrak dilakukan dengan kromatografi lapis tipis (KLT). Konsentrasi ekstrak yang digunakan untuk membuat krim tabir surya adalah 0,250% ; 0,500% dan 1,000%. Evaluasi yang dilakukan pada sediaan adalah uji mutu fisik, uji stabilitas sediaan dan aktivitas tabir surya baik in vivo dengan uji iritasi pada kulit manusia dan in vitro dengan spektrofotometri UV-Vis.
Hasil penelitian ekstrak etanol daun stroberi didapatkan rendemen sebanyak 20,5% dan uji KLT menunjukan bahwa ekstrak daun stroberi positif mengandung flavonoid dan tanin. Krim ekstrak etanol daun stroberi yang paling baik pada konsentrasi 1,000% dengan nilai SPF sebesar 52,90
Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Johar (Cassia siamea Lamk.) dan Daun Alpukat (Persea americana Mill.) terhadap Salmonella Typhi
Get PDFTyphoid fever is an acute febrile disease caused by Salmonella typhi bacteria, WHO 2018 data states that in the world it reaches 11-20 million cases per year, in Indonesia it reaches a prevalence rate of 358-810/100,000 population. The purpose of this study was to determine the antibacterial activity of the combination of johar leaves (Cassia siamea Lamk) and avocado leaves (Persea americana Mill) against the growth of salmonella typhi bacteria.
Testing the antibacterial activity of a combination of 1:1 70% ethanol extract of johar leaves and avocado leaves by diffusion and dilution methods, treatment using control (+) ciprofloxacin and control (-) DMSO 2% with series concentrations of 25, 50, 75 and 100%. Each treatment was repeated 3 times.
The results of the antibacterial activity test of the combination of ethanol extract of johar leaves and avocado leaves had the greatest activity against Salmonella typhi bacteria at a concentration of 100% with a clear zone diameter of 9.9 mm while the smallest average was at a concentration of 25% with a diameter of 6 mm, and ciprofloxacin with a diameter of 29.3 mm. The minimum inhibition level was at a concentration of 12.5%.Demam tifoid merupakan penyakit demam akut yang disebabkan bakteri Salmonella typhi, 11-20 juta kasus setiap tahun dari laporan WHO 2018, 358-810/100.000 penduduk untuk negara Indonesia. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri kombinasi daun johar (Cassia siamea Lamk) dan daun alpukat (Persea americana Mill) terhadap pertumbuhan bakteri salmonella typhi.
Pengujian aktivitas antibakteri kombinasi 1:1 ekstrak etanol 70% daun johar dan daun alpukat dengan metode difusi dan dilusi, perlakuan menggunakan control (+) ciprofloxacin dan control (-) DMSO 2% dengan seri konsentrasi 25, 50, 75 dan 100 %. Masing-masing perlakuan dilakukan pengulangan sebanyak 3 kali.
Hasil uji aktivitas antibakteri kombinasi ekstrak etanol daun johar dan daun alpukat memiliki aktivitas terhadap bakteri Salmonella typhi paling besar pada konsentrasi 100% dengan diameter zona bening 9.9 mm sedangkan terkecil rata-rata pada kosentarasi 25% diameter 6 mm, dan siprofloksasin dengan diameter 29,3 mm. Kadar hambat minimum pada konsentrasi 12,5%
Pengaruh Edukasi Terhadap Pengetahuan Swamedikasi Batuk pada Anak di Kecamatan Wonosari Kabupaten Klaten Jawa Tengah
Get PDFSelf-medication is one way people treat a symptom of a disease without using a doctor's prescription. Knowledge of correct self-medication is needed to cure coughs without other adverse effects on the sufferer. This study aimed to determine the effect of education through online media on the level of knowledge about cough self-medication for children in Wonosari District, Klaten Regency, Central Java in 2020.
This research is a quantitative study with a Quasi-Experimental research method with the technique of Two Group Pre-Test-Post-Test Control Group Design. The population in this study were parents who had children in Wonosari District, Klaten Regency. The sample in this study amounted to 268 people. The data collection tool uses a questionnaire that has been tested for validity and reliability.
The results showed that the level of public knowledge when the pre-test was carried out was classified as good (41.04%), moderate (54.48%), poor (4.48%), not good (0%). The provision of education was able to increase the respondent's knowledge as indicated by the results of post-test knowledge, which were classified as good (89.55%), moderate (10.45%), not good (0%), poor (0%). The Wilcoxon test analysis obtained a p-value of 0.000 000 <α value of 0.05, which means that education through online media affects public knowledge about cough self-medication in children. Pengobatan sendiri atau swamedikasi merupakan salah satu cara masyarakat untuk mengobati suatu gejala penyakit tanpa menggunakan resep dari dokter. Pengetahuan akan swamedikasi yang benar sangat diperlukan agar batuk dapat disembuhkan dengan tidak disertai efek lain yang merugikan penderita. Tujuan dari penelitian ini untuk mengetahui pengaruh edukasi melaui media online terhadap tingkat pengetahuan masyarakat tentang swamedikasi batuk pada anak di Kecamatan Wonosari, Kabupaten Klaten, Jawa Tengah pada tahun 2020.
Penelitian ini merupakan penelitian kuantitatif dengan metode penelitian Quasi Eksperimental dengan teknik Two Group Pre Test-Post Test Control Group Design. Populasi dalam penelitian ini adalah orang tua yang memiliki anak di Kecamatan Wonosari Kabupaten Klaten. Sampel dalam penelitian ini berjumlah 268 orang. Alat pengambilan data menggunakan kuesioner yang telah di uji validitas dan reliabilitasnya.
Hasil penelitian menunjukan bahwa tingkat pengetahuan masyarakat padasaat dilakukan pre-test tergolong baik (41,04%), cukup (54,48%), kurang (4,48%), tidak baik (0%). Pemberian edukasi mampu meningkatkan pengetahuan responden ditunjukan dengan hasil pengetahuan post-test tergolong baik (89,55%), cukup (10,45%), tidak baik (0%), kurang (0%). Analisis uji Wilcoxon didapatkan nilai p 0,000 000 < nilai α 0,05 yang dapat diartikan bahwa pemberian edukasi melalui media online mempengaruhi pengetahuan masyarakat mengenai swamedikasi batuk pada anak
Platelet Morphological Assessment Based on Shelf-Life of Concentrated Platelet Components
Get PDFConcentrated platelet components are the components or platelets that are processed through centrifugation from whole blood or a single donor. The components of the concentrate platelets are stored in an agitator at a temperature of 20-24◦C with a maximum shelf-life of five days. During the storage, a change in component metabolism occurs, which is characterized by alteration in platelet morphology as an indicator of decreasing quality of platelet components. The purpose of this study was to determine the quality of the components of platelets based on the shelf-life by measuring the platelet morphological values with the Kunicki method. This study applied an experimental method by conducting inspections of the regional blood transfusion unit (UTDD) of the Indonesian Red Cross of Jakarta Capital Special Region (PMI DKI Jakarta). Platelet morphological values of six components of platelets were examined by the Kunicki method on day 0 to day 5 of shelf-life. Quality assessment was carried out based on a significant difference-dependent t-test and comparison with normal value. The results of the study by performing statistical analysis of the significant difference-dependent t-test showed a significant difference of values between day 0 and day 1 to day 5 of shelf-life. Comparison with normal values depicted that all samples stored on day 4 had a morphological value of more than 200. On day 5 of shelf-life, the platelet morphology values of five samples were below 200. The quality of the concentrated platelet components is said “good” if it is above 200. This study concluded that there was a significant difference in the platelet morphology values between the components of concentrated platelets on day 0 and day 1 to day 5 of shelf-life. All samples were of good quality until day 4 of storage. However, on day 5 of storage, only one sample was good in quality.Concentrated platelet components are the components or platelets that are processed through centrifugation from whole blood or a single donor. The components of the concentrate platelets are stored in an agitator at a temperature of 20-24◦C with a maximum shelf-life of five days. During the storage, a change in component metabolism occurs, which is characterized by alteration in platelet morphology as an indicator of decreasing quality of platelet components. The purpose of this study was to determine the quality of the components of platelets based on the shelf-life by measuring the platelet morphological values with the Kunicki method. This study applied an experimental method by conducting inspections of the regional blood transfusion unit (UTDD) of the Indonesian Red Cross of Jakarta Capital Special Region (PMI DKI Jakarta). Platelet morphological values of six components of platelets were examined by the Kunicki method on day 0 to day 5 of shelf-life. Quality assessment was carried out based on a significant difference-dependent t-test and comparison with normal value. The results of the study by performing statistical analysis of the significant difference-dependent t-test showed a significant difference of values between day 0 and day 1 to day 5 of shelf-life. Comparison with normal values depicted that all samples stored on day 4 had a morphological value of more than 200. On day 5 of shelf-life, the platelet morphology values of five samples were below 200. The quality of the concentrated platelet components is said “good” if it is above 200. This study concluded that there was a significant difference in the platelet morphology values between the components of concentrated platelets on day 0 and day 1 to day 5 of shelf-life. All samples were of good quality until day 4 of storage. However, on day 5 of storage, only one sample was good in quality
The Analysis of Vitamin D Receptor Protein on Salmonella typhi infection in acute recurrent cases in endemic area in Eastern Indonesia
Get PDFThe host susceptibility mechanisms such as Vitamin D Receptor (VDR) is involved in the modulation of macrophage function and may possibly correlate with immunity disease including the severity of typhoid fever symptoms. The study aimed to assess the VDR Protein expression in the serums of recurrent acute typhoid fever (RATF) patients and compares it with typhoid fever (TF) patients, and healthy persons (HP). The study employed 30 RATF patients and 30 TF patients selected from primary health centres and hospitals in Eastern Indonesia as the endemic area. All the samples were obtained from several health centers in South Sulawesi, Southeast Sulawesi, Central Sulawesi, East Kalimantan and Papua and then collected in the sample bank Biology Laboratory of Molecular Immunology, Faculty of Medicine, Hasanuddin University. As a comparison, 30 samples of healthy persons were also selected from the Blood Transfusion Unit in Makassar, South Sulwesi Indonesia. The profile of VDR Protein was analyzed with Enzyme-linked Immunosorbent Assay (ELISA). VDR protein content data on RATF and TF were designed according to completely randomized design T test. Subsequently, it correlated to Pearson correlation to determine the interaction between Widal titre and VDR protein levels. A comparison between Widal titre and VDR Protein level was also made to identify the correlation. It was found that the mean of VDR protein expression of RATF was 13,44 ng/mL, the mean of VDR protein expression of TF was 24,88 ng/mL, and the mean of VDR protein expression of HP was 43,49 ng/mL. The correlation results between RATF-TF Widal titre and VDR protein level indicated a negative correlation with p-value of 0,004. There were significant differences in the VDR expression in the RATF, TF, and HP. RATF VDR expression lower than TF and HP and there was also a correlation between Widal titre with VDR Protein expression.The host susceptibility mechanisms such as Vitamin D Receptor (VDR) is involved in the modulation of macrophage function and may possibly correlate with immunity disease including the severity of typhoid fever symptoms. The study aimed to assess the VDR Protein expression in the serums of recurrent acute typhoid fever (RATF) patients and compares it with typhoid fever (TF) patients, and healthy persons (HP). The study employed 30 RATF patients and 30 TF patients selected from primary health centres and hospitals in Eastern Indonesia as the endemic area. All the samples were obtained from several health centers in South Sulawesi, Southeast Sulawesi, Central Sulawesi, East Kalimantan and Papua and then collected in the sample bank Biology Laboratory of Molecular Immunology, Faculty of Medicine, Hasanuddin University. As a comparison, 30 samples of healthy persons were also selected from the Blood Transfusion Unit in Makassar, South Sulwesi Indonesia. The profile of VDR Protein was analyzed with Enzyme-linked Immunosorbent Assay (ELISA). VDR protein content data on RATF and TF were designed according to completely randomized design T test. Subsequently, it correlated to Pearson correlation to determine the interaction between Widal titre and VDR protein levels. A comparison between Widal titre and VDR Protein level was also made to identify the correlation. It was found that the mean of VDR protein expression of RATF was 13,44 ng/mL, the mean of VDR protein expression of TF was 24,88 ng/mL, and the mean of VDR protein expression of HP was 43,49 ng/mL. The correlation results between RATF-TF Widal titre and VDR protein level indicated a negative correlation with p-value of 0,004. There were significant differences in the VDR expression in the RATF, TF, and HP. RATF VDR expression lower than TF and HP and there was also a correlation between Widal titre with VDR Protein expression
HUBUNGAN INTERAKSI SOSIAL DAN HARGA DIRI DENGAN KOHESIVITAS KELOMPOK PADA SISWA SEKOLAH SEPAKBOLA (SSB) DI KUDUS
Get PDFThis study aims to determine the relationship of social interaction and self-esteem with group cohesiveness of SSB students in Kudus. The subjects of this study were 80 students of SSB in Kudus. The sampling technique in this study used a quota sampling technique. The measuring tools used to obtain the data are the social interaction scale, self-esteem scale, and group cohesiveness scale. The results of data analysis using Regression Analysis where the calculation uses the SPSS 15.0 program. The results of the correlation coefficient rx1,2y of 0.836 with p of 0.000 (p<0.01) mean that there is a very significant relationship between social interaction and self-esteem with group cohesiveness. The result of the correlation coefficient between social interaction variables and group cohesiveness rx1y is 0.836 with p of 0.000 (p <0.01) this means that there is a very significant positive relationship between social interaction and group cohesiveness. While the correlation coefficient between the variable self-esteem and group cohesiveness rx2y is 0.708 with p of 0.000 (p <0.01) this means that there is a very significant positive relationship between self-esteem and group cohesiveness. The effective contribution of the independent variable to the dependent variable is 69.9%.Penelitian ini bertujuan untuk mengetahui hubungan interaksi sosial dan harga diri dengan kohesivitas kelompok pada siswa SSB di Kudus. Subjek penelitian ini adalah 80 siswa SSB di Kudus. Teknik pengambilan sampel pada penelitian ini menggunakan teknik kuota sampling. Alat ukur yang digunakan untuk memperoleh data adalah skala interaksi sosial, skala harga diri, dan skala kohesivitas kelompok. Hasil analisis data dengan menggunakan Analisis Regresi dimana perhitungan menggunakan program SPSS 15.0. Diperoleh hasil koefisien korelasi rx1,2y sebesar 0,836 dengan p sebesar 0,000 (p<0,01) ini berarti ada hubungan yang sangat signifikan antara interaksi sosial dan harga diri dengan kohesivitas kelompok. Hasil koefisien korelasi antara variabel interaksi sosial dengan kohesivitas kelompok rx1y sebesar 0,836 dengan p sebesar 0,000 (p<0,01) ini berarti ada hubungan positif yang sangat signifikan antara interaksi sosial dengan kohesivitas kelompok. Sedangkan koefisien korelasi antara variabel harga diri dengan kohesivitas kelompok rx2y sebesar 0,708 dengan p sebesar 0,000 (p<0,01) ini berarti ada hubungan positif yang sangat signifikan antara harga diri dengan kohesivitas kelompok. Sumbangan efektif variabel bebas terhadap variabel terikat sebesar 69,9%