Journal of Tropical Pharmacy and Chemistry
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Aktivitas Sediaan Gel Antiseptik Tangan Berbahan Aktif Ekstrak Fraksi Etanol Daun Sungkai (Peronema canencens Jack.) terhadap Beberapa Bakteri Patogen
Get PDFA research which antiseptic hand gel preparations containing the active leaf extract fraction ethanol Sungkai (Peronema canencens Jack.) has been done. This study aims to know is antiseptic activity Sungkai leaf fraction (P. canescens. Jack) in vitro against to several microbial pathogens in the preparation of antiseptic hand gel, and to know determine the best concentration of ethanol fraction Sungkai leaf in the preparation of antiseptic gel hands each strain of pathogenic bacteria. Test materials obtained by fractionation of the ethanol extract of the leaf fraction Sungkai, further formulated into a gel base antiseptic, tested its activity against several bacterial pathogens, and determine the best concentration of ethanol fraction Sungkai leaf in the preparation of antiseptic hand gel against Staphylococcus aureus, Salmonella thyposa, Escherichia coli and Bacillus subtilis bacteria. Antibacterial testing using method in vitro solid diffusion test. The results showed the concentration of ethanol fraction in gel dosage of active antiseptic against Staphylococcus aureus, Salmonella thyposa, Escherichia coli and Bacillus subtilis bacteria. The best concentration of ethanol extract in the preparation of antiseptic gel was 4% effective at inhibiting or kill the to three bacteria.
Bioassay-Guided Fractination of Antimitotic Compound from Ongkea Cortex (Mezzettia parviflora Becc) Towards Sea Urchin Eggs
Get PDFOngkea cortex, the wood bark of Mezzettia Parviflora Becc, is a traditional medicine originated from Southeastern Sulawesi (Indonesia). It has been empirically known to have antitumor property. In this study, we examined the antiproliferative activity and obtained the antimitotic compound of ongkea cortex. Antimitotic activity was ultimately determined by the inhibition of cleavage-stage of newly fertilized sea urchin (Lytechinus variegatus) eggs. A bioassay-guided fractination was performed in order to find the bioactive substance of ongkea cortex. The IC50 values of methanolic extract, ethyl acetate-soluble part of metanolic extract and ethil acetat insoluble part of metanolic extract were 1221.68 µg/mL, 2.69 µg/mL, and 15.15 µg/mL, respectively. Ethyl acetate-soluble part of metanolic extract was further investigated. It was partitionated using vacuum liquid column chromatoghraphy with different solvent system by increasing their polarities. There were three different fractions obtained. Fraction III exerted the highest inhibition activity with IC50 value of 1.33 µg/mL. It was separated subsequently to result four groups of compounds. III-C group presented the most potent inhibition activity with IC50 value of 0.7147 µg/mL. It was then subjected to preparative TLC and yieldedsix groups of subfractions. III-C-3 subfraction was indicated as the most potent compound with IC50 value of 0.3378 µg/mL. It was ten times weaker compared with antimitotic activity of Vincristine with IC50 of 0.0351 µg/mL. As a conclusian, ongkea cortex might have antimitotic property with the highest rate inhibition activity exhibited by III-C-3 compound
Aktivitas Antijamur dan Identifikasi Metabolit Sekunder Isolat Jamur Endofit dari Daun Yakon (Smallanthus sonchifolius) terhadap Beberapa Jamur Patogen
Get PDFEach plant is host to one or more endophytes contain bacteria and fungi. Endophytic fungi could potentially have an activity and produce secondary metabolites same as its host. Yacon leaf (Smallanthus sonchifolius) has been known to have antimicrobial activity that allow the endophytic fungi also has potential as antimicrobial. The purpose of this research was to isolated the endophytic fungi from Yacon leaf which has activity as antifungal and can produce secondary metabolites same as its host. The method that used to test the activity of endophytic fungi are using direct planting method, whereas the identification of secondary metabolites using Thin Layer Chromatography (TLC) and spray reagent. The results were obtained that the endophytic fungi has an activity as antifungal against Candida utilis, Candida albicans and Malassezia Purpur, along with the presence of secondary metabolites that same as its host
Formulasi Granul Ekstrak Kulit Buah Manggis (Garcinia mangostana. L) Menggunakan Aerosil dan Avicel PH 101
Get PDFSalah satu tanaman Indonesia yang bisa dimanfaatkan adalah buah manggis (Garcinia mangostana L.). Banyak penelitian yang dilakukan dengan ekstrak kulit manggis terhadap aktivitas farmakologi seperti antiinflamasi, antihistamin, antioksidan dan antimikroorganisme. Variasi pengolahan kulit buah manggis pada masyarakat Indonesia masih terbatas sehingga diperlukan pengembangan dalam bentuk sediaan lain seperti dibuat menjadi granul. Penelitian ini bertujuan untuk mengetahui apakah granul ekstrak kulit buah manggis menggunakan aerosil dan avicel PH 101 memenuhi persyaratan fisik granul yang baik dan untuk mengetahui konsentrasi aerosil dan avicel PH 101 yang memenuhi persyaratan fisik granul ekstrak kulit buah manggis. Penelitian diawali dengan proses ekstraksi simplisia kulit buah manggis secara maserasi dengan menggunakan pelarut etanol 70% dan diuapkan hingga diperoleh ekstrak kental. Pembuatan granul dari ekstrak kulit manggis dilakukan dengan menggunakan kombinasi antara aerosil dan avicel 101 yang divariasikan dalam 4 formula yaitu formula 1 aerosil : avicel 101(0% : 20%), formula 2 aerosil : avicel 101% (20% : 20%), formula 3 aerosil : avicel 101 (0% : 60%) dan formula 4 aerosil : avicel 101 (20% : 60%). Tahap selanjutnya granul hasil dari formulasi dievaluasi sifat fisik granul meliputi uji kandungan lembab, densitas massa, ukuran partikel dan sifat alir. Hasil yang diperoleh dianalisis secara statistik menggunakan analisis variasi (ANAVA). Hasil penelitian menunjukkan dengan konsentrasi avicel 101 : aerosil (20% : 0%) dan konsentrasi avicel 101 : aerosil (60% : 0%) granul ekstrak kulit manggis, memenuhi persyaratan, meliputi uji kandungan lembab, densitas massa, ukuran partikel dan sifat alir. Hal ini menunjukkan bahwa avicel 101 mampu berperan sebagai pengikat yang baik dan kuat sekaligus sebagai penyerap cairan pada granul
Uji Sitotoksik dan Uji Kombinasi Fraksi Etil Asetat Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia Jack.,) dan Doksorubisin pada Sel Limfosit
Get PDFThe cytotoxicity and combination test of ethyl acetate fraction of ethanolic extract of Pasak bumi roots (Eurycoma longifolia Jack.) and doxorubicin have been made to the normal lymphocyte cells in vitro. The tests were carried out by using MTT method. Principle of the MTT method is to measure the mitochondrial dehydrogenase activity in living cells that have the ability to convert MTT into formazan. Cytotoxicity test for fraction performed at concentrations of 2000;1000;500;250;125;62,5;31,25 μg/mL and the concentrations of doxorubicin at 4;2;1;0,5;0,25;0,125;0,0625 μg/mL. From the tests IC50 values obtained ethyl acetate fraction of ethanolic extract of pasak bumi roots and doxorubicin against lymphocyte cells each 44 μg/mL and 1.1 μg/mL. The results of combination index (CI) value is 73,282 (CI>1= antagonist) at concentration of the combination 22 μg/mL for ethyl acetate fraction and 0,5547 for doxorubicin. The Results showed ethyl acetate fraction of ethanolic extract of the pasak bumi roots has lower toxicity than doxorubicin and it can be used for the combination chemotherapy with doxorubicin
Pengaruh Waktu Pengambilan Sampel Darah terhadap Pemeriksaan Testosteron
Get PDFThe research about “The Influence of Sampling Time to Testosterone for Medical Check Up†had been develoved to 19 man aged 19 – 25 years old. Sample was collected at 8 a.m. and 12 a.m. to determine testosterone serum level. Determining of testosteron serum level was done using Electrochemiluminescence Immunoassay(ECLIA). The result show that testosterone serum level mean at 8 a.m. (608.90 ng/dL) is slightly lower than testosterone serum level at 12 a.m. (647.95 ng/dL). The analysis of t test (α=0.05) show that there was not significantly difference between testosteron level mean at 8 a.m. and 12 a.m. So the sampling time to determine testosteron serum level to men can be done until 12.00 a.m
Pengaruh Penambahan Kulit Jeruk Bali (Citrus maxima) terhadap Kualitas Minyak Goreng yang Mengalami Pemanasan
Get PDFCooking oil is used to fry foods. The longer and more frequent the cooking oil being used, more its quality is getting lower. The goal of this research is to know if there is an effect of adding grapefruit peels due to the quality of cooking oil that has been used several times. Grapefruit peel contains lycopene which can reduce oxidation process. By adding grapefruit peel, we hope its can reduce the lowering quality. The orientation shows that 3% grapefruit peel weight/weight is the most efficient concentration to be used. And with 3% grapefruit peel weight/weight, the results shows a big difference between cooking oil with no grapefruit peel addition and cooking oil with grapefruit peel addition
Evaluasi Formula Krim Minyak Biji Delima (Punica granatum L.) dan Uji Aktivitas Antioksidan dengan Metode β-Carotene Bleaching
No full textPomegranate seed oil has a potent antioxidant activity that is potential to be formulate into topical antioxidant dosage form. The purpose of this study is to formulate cream of pomegranate seed oil that physically stable, however have antioxidant activity. Cream w/o was formulated using Tween 80 - Span 80 as emulsifier. Evaluation of cream products includes determining pH and viscosity of the preparations stored for 4 weeks at room temperature, physical stability test by Freeze Thaw method which was carried out for 4 cycles in where 1 cycle consists of 48 hours at 4°C and 48 hours at 40°C. Antioxidant activity was tested by β-carotene bleaching method. The results of the research showed that all cream formulas containing pomegranate seed oil 1% were physically stable and can increase the comfortness in use on the skin. The antioxidant test results showed antioxidant activity of pure pomegranate seed oil and pomegranate seed oil as formulated in creams have antioxidant capacity with inhibition percentage of 73.41% and 52.80%, respectively. Based on these results it can be concluded that the antioxidant activity of pomegranate seed oil in cream decreased 0,3 times
Aktivitas Antibakteri dari Fraksi Daun Bandotan (Ageratum conyzoides L.) secara Kromatografi Lapis Tipis Bioautografi
Get PDFScreening Ageratum conyzoides L. fraction for antibacterial activity using thin layer chromatography bioautography have been done. The method is based on thin-layer chromatography–direct bioautography of Ageratum conyzoides chloroform fraction. The aim of study to determine the antibacterial activity of chloroform Ageratum conyzoides L. fraction in TLC bioautography. Extraction was done by maceration using methanol solvent, followed by partition using chloroform solvent using liquid-liquid extraction method is done by chromatographic profile of each eluent comparison is then performed further fractionated using vacuum liquid chromatography method. Screening activity of the compound was done qualitatively raction results are antibacterial activity test using thin layer chromatography bioautography The results showed that the eluent to separate the components of a compound based on the chomatography profile is eluent n-hexane: ethyl acetate (2: 1), the results obtained by fractionation of the active fraction (51-53) Rf value of 0.59. Results of screening activity held chloroform fraction Ageratum conyzoides leaves are antibacterial activity which can be seen from the formation of inhibition zones in the region stain spot on the TLC plate were grown in glucose medium Nutrient. The results were analyzed descriptively, that is to describe the characteristics of the research which is obtained
Isolasi dan Identifikasi Bakteri Lamun Enhalus Acoroides Di Perairan Pasarwajo Buton
Get PDFThis research aims to isolation bacteria in segrass Enhalus acoroides 1) determination optimum growth factor of bacteria that symbiont in seagrass 2) identification kind of bacteria that isolated in seagrass. Base on the research that has done, the results show that bacteria symbiotically in seagrass grow in optimum temperature is 27 °C in 10-3 dilution. Identification characteristic, morphology and gram colouring bacteria that symbionts in seagrass is gram negatif bacteria that showed with result of PCR which species isolated in seagrass is enterobacter cloaceae