Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
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Serapan Fosfor dan Pertumbuhan Kedelai(Glycine max) pada Tanah Ultisol dengan Pemberian Asam Humat
Get PDFUltisol merupakan salah satu jenis tanah asam yang memiliki kelarutan Al, Fe, dan Mn tinggi, serta kandungan P dan Moyang rendah. KelarutanAl dan Fe yangtinggiakan menjerap fosfat, sehingga ketersediaan dan serapan P bagi tanaman menjadi rendah. Salah satu upaya untuk mengatasi ketidaktersediaan P pada tanah Ultisol yaitu dengan penambahan asam humat. Asam humat merupakan makromolekul polielektrolit yang memiliki gugus fungsional seperti -COOH, -OH fenolat, maupun -OH alkoholat,sehingga asam humat memiliki kemampuan untuk membentuk kompleks dengan ion logam. Penelitian ini dilakukan dengan tujuan untuk: (1) mengetahui pengaruh asam humat terhadap serapan P dan pertumbuhan tanaman kedelai (2) menentukan konsentrasi asam humat yang paling baikdalam meningkatkan serapan P dan pertumbuhan tanaman kedelai. Metode penelitian yang digunakan adalah eksperimental dengan Rancangan Acak Kelompok (RAK). Pengelompokan didasarkan atas perbedaan intensitas cahaya yang ada di rumah kaca. Perlakuan berupa konsentrasi asam humat, dengan 5 konsentrasi yang berbeda yaitu 0 ppm, 400 ppm, 800 ppm, 1200 ppm dan 1600 ppm. Parameter yang diukur meliputi tinggi tanaman, luas daun, bobot basah, dan bobot kering, P tersedia serta P tanaman. Data yang diperoleh dianalisis menggunakananalisis ragam pada tingkat kepercayaan 95% dan 99%. Apabila hasil perlakuan berpengaruh nyata, maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa asam humat berpengaruh terhadap serapan P dan pertumbuhan kedelai pada tanah Ultisol. Asam humat 1200 ppm merupakan perlakuan paling baik dalam meningkatkan ketersediaan P, sehingga mampu meningkatkanpertumbuhan kedelai pada tanah Ultisol
Aktivitas Immunodulator Ekstrak Etanol Umbi Bawang Merah (Allium cepa L.) terhadap Respon Imun Non Spesifik pada Mencit Jantan Galur BALB/C dengan Metode Carbon Clearance
Get PDFThe content of flavonoids on onion useful to maintain endurance. This research aims to know the activity of exstract immunodulator of onion bulb ethanol (Allium cepa L) on phagocytic activity in male mice balb/c with varian dose and campored immunodulator activity with positive control. This study is an experimental reseach with Completely Randomized Design (CRD). Preparation of extract ethanol onion roots done by maceration using 96% ethanol for 9 hours. The test performed on samples which consist of 25 mice with carbon clearance method, devided into 5 groups, which are : K – (without treatment), k+ (with imboos 13,3 ml/Kg Bw), P1 (dose 12%), P2 (dose 24%), and P3 (dose 48%). The test performed for 5 days, and on the 7th day injected carbon (pelicans) as much 0,1 ml/ 10 gr Weight intravenousty, their blood taken before carbon injection (minute 0) and minute 4, 8, 12, and 16. After their transmittance measure using spectrophotometer at 600,5 nm wavelength. This research shows that extract ethanol of onion balb has immunodulator activity effect on non-specific immune response on male mice balb/c with carbon clearance method. The dose of 12% (v/v), 24% (v/v) and 48% (v/v) dose have lower immunostimulatory capacity than positive control
Biosorpsi Krom Total dalam Limbah Cair Batik dengan Biosorben yang Dikemas dalam Kantung Teh Celup
Get PDFKrom dalam limbah cair batik dapat dikurangi dengan metode biosorpsi menggunakan biosorben yang murah, melimpah dan mudah didapat yaitu S. cinereum dan limbah baglog P. ostreotus. Kelemahan ukuran partikel yang kecil adalah sulit dipisahkan dari limbah ketika diaplikasikan, sehingga diperlukan sebuah bentuk atau kemasan biosorben yang memudahkan proses aplikasinya yaitu mengemas dalam kantung teh celup. Tujuan penelitian adalah mendapatkan perbandingan komposisi dan ukuran partikel yang optimum menyerap krom total pada limbah cair batik. Penelitian dilakukan secara eksperimental dengan rancangan petak terpisah. Data yang diperoleh dianalisis menggunakan analisis varian dengan tingkat kesalahan 5%. Hasil penelitian menunjukkan persentase adsorpsi tertinggi terdapat pada perbandingan 3:1 μm sebesar 62.69%. Biosorben yang dikemas dalam kantung teh celup efektif menurunkan krom pada limbah cair batik
TITER EKDISON LOBSTER HIJAU PASIR (Panulirus homarus L.) PADA FASE PREMOLTING AKHIR
Get PDFSpiny Lobster, Panulirus homarus, farming is very potential to be developed in Lombok Island, but physiological aspects such as nutrient needs and growth hormone, especially ecdysone titer, were little known. This research aimed to determine the ecdysone titer of Panulirus homarus during the last pre-molting phase. This study was conducted as a quasi-experiment with one group post-test design. The lobsters used in this experiment have the length of carapacs and the body weight of 1.5–2.0 cm and 4.33–5.77 g respectively. The lobster fed for 42 days with gel pellets three times a day before ecdysone titer measurement. The ecdysone hormone was then isolated from the second segment of the abdomen on the basal of the fifth pereiopod. Ecdyson titer was measured by using High-Performance Liquid Chromatography (HPLC). The result showed that the titer of ecdysone was 5.27 µg/ml hemolymph. The titer of ecdysone was found higher compared to previous research on Homarus americanus; this result suggested that the spiny lobster Panulirus homarus needs a higher amount of ecdysone hormone to initiate the molting process
AKTIVITAS PROTEASE DAN AMILASE PADA IKAN SIDAT Anguilla bicolor McClelland)
Get PDFThis study was experimental, conducted using a Completely Randomized Design (CRD) with 3 x 2 factorial design, and four replicates have been carried out to evaluate the protease and amylase activities of Anguilla bicolor McClelland. A total of 71 individuals divided into three weight groups were used in this study. The first group with an average weight of 41.25 ± 0.898 g consisted of 51 eels, the second with an average weight of 319.8 ± 4.666 g composed of 14 eels, and the third with a mean weight of 569.5 ± 9.150 g consisted of 6 eels. The results showed the protease activity differed significantly based on eel size and intestinal segment (P ˂ 0.05). This research recorded the highest protease activity was in eels within the smallest weight group (41.25 ± 0.898 g). This study also revealed the protease activity in the anterior intestine was higher than the posterior in all size of eels. The amylase activity did not differ significantly (P>0.05) by eel size and intestinal segment. This study concluded the protein digestion capacity of smaller eels was higher than larger eels, and the protein digestion capacity was greater in the anterior intestine than the posterior intestine. The carbohydrate absorption capacity in eel was not affected by the variety of fish size which indicates no change in the feed category
PERUBAHAN KADAR PROTEIN DAN STATUS LIPOSTATIK IKAN SIDAT, Anguilla bicolor, STADIA SILVER YANG DIPELIHARA PADA SALINITAS YANG BERBEDA
Get PDFEel (Anguilla bicolor) is a catradomous fish, in the growth phase of life in freshwater and upon reaching adult will migrate to ocean waters to reproduce. Eel during the migration will use energy from body reserves to osmoregulation. Increased salinity will affect the osmotic pressure of the body of the fish so that the fish do active transport which requires energy. Energy that used for osmoregulation approximately 20–50% of the total energy from carbohydrates, fats and proteins. Carbohydrates are the first source of energy that is used and then will be replaced by fat and protein as energy after carbohydrate depleted. Thus the energy utilization during osmoregulation will affect the changes in the composition of protein and fat content of the body. Fat content will affect the value of lipostatic fish is one of the growth parameters. The purpose of this study was to evaluate changes in the levels of protein and lipostatic eel (Anguilla bicolor) is maintained in a range of salinity. This research method is experimental research design with CRD, treatment in the form of maintenance medium salinity; 4 ppt (control), 15 ppt, and 30 ppt. Each treatment was repeated 5 times. Test fish used were obtained from the Village Pesanggrahan Cilacap with range size between an average weight of 395–920 g and an average length of 59–81 cm. The variables measured were the levels of protein and lipostatic eel. Protein content measurement was conducted using kjeldahl and the fat content by soxhlet. Research results showed that the eels were exposed to salinity 4 ppt, 15 ppt and 30 ppt no effect on protein content and body lipostatic eel. It can be concluded that the eel is able to adapt well in the range between 4 ppt salinity to 30 ppt
ANALISIS KONDISI HABITAT DAN PERAKARAN TUMBUHAN BAWAH PADA DAERAH TERBUKA DAN DI BAWAH TEGAKAN CEMARA UDANG DI PESISIR LEMBUPURWO, KEBUMEN
Get PDFCoastal areas with sandy soil structure without trees have high temperature and salinity, strong wind, as well as minimum nutrient content. The limitation of the environment conditions affects the variety of undergrowth. This research aims to figure out the composition, distribution patterns, habitat conditions and rooting from undergrowth species which are found in coastal sandy areas at Lemburpurwo. The research was conducted using line transect method defined by systematic sampling with random start. The observations of undergrowth were carried out on 3 types of treads near from the sea, under and behind Cemara Udang stands. Paths are made perpendicular within approximately 53 m from the shoreline and the distance between lines is 95 m. Each track was divided into segments in the form of 2x2 m2 plots which were laid out diagonally between plots for undergrowth observation. 81 plots were taken, where the size of the area is 72960 m2. Undergrowth plant species were identified, the length and number of roots were measured, environmental conditions and soil quality including pH, DHL, N total content, P and K available were observed. Lembupurwo coastal sandy area consists of 20 species of 13 plant families with 3 types of random distribution pattern and 17 types of clustered distribution pattern. Spinifex littoralis has the longest root as well as the largest number of roots, especially in open areas. The pH values and electrical conductivity below the stands of 6.41; 32,23 µs/cm are lower when compared to locations near the sea. N-total and P available under stands are available 0,03%; 5,05 ppm which is higher than the other two locations
Kelimpahan dan Potensi Biofuel Mikrofitobenthos dari Perairan Sungai Pekacangan yang terkena Limbah Cair Tapioka
Get PDFMicrophytobenthic a benthic plants, found in the waters of the sunlight reaching the bottom waters. In addition to acting as a primary producer also has the potential to produce renewable biofuels The purpose of this study was to determine the microphyitobenthic abundance of river Pekacangan affected tapioca liquid industrial waste and to estimate its potential as a biofuels. This research used survey method with the sampling technique is purposive sampling based on existing environmental setting. As the main parameters are the types of microphytobenthic, while supporting research parameters are temperature, light penetration, pH, dissolved O2, BOD5, current velocity, TSS, TDS, nitrate, orthophosphate and silica. Microphytobenthic obtained at stations affected by the waste, in screening to determine its oil content.Abundance of microphytobenthic at a station directly exposed tapioca liquid waste 620 ind.mm-2.Isolated microphytobenthic River Pekacangan affected tapioca liquid waste had 22 isolates of microphytobenthos and after screened allegedly 7 isolates potential as a biofuel, namely Navicula sp., Amphora libyca, Ulothrix sp., Anabaena sp., Planctonema sp., Cymbella sp., and Eunotia sp
Ekspresi mRNA LMP2A Epstein-Barr Virus dari Biopsi Jaringan dalam Blok Parafin Berpotensi sebagai Biomarka dalam Diagnosis Karsinoma Nasofaring
Get PDFKarsinoma nasofaring (KNF) merupakan tumor epitel yang terletak di nasofaring dan merupakan penyakit genetik multifaktor yang endemik. Penyebab utama KNF adalah infeksi oleh Virus Epstein-Barr (EBV). Keberadaan EBV pada penderita KNF dapat diketahui dengan ditemukannya DNA EBV dalam spesimen biopsi jaringan penderita KNF. Genom EBV dan ekspresi sebagian dari produk gen laten virus secara konsisten terdeteksi hampir di setiap sel dalam kanker ini, salah satunya adalah gen Latent Membrant Protein (LMP). Aktivitas mRNA EBV lebih mencerminkan patogenesis KNF yang sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi. Penelitian dilakukan di Laboratorium PA RSUD Margono Seokarjo, Purwokerto/Lab PA Fakultas Kedokteran dan laboratorium genetika/molekuler Universitas Jenderal Soedirman Purwokerto. Analisis Ekspresi mRNA LMP2A EBV dengan teknik one step RT-PCR dan produk RT-PCR (amplikon cDNA) divisualisasi dengan elektroforesis gel agarosa 1%. Hasil ekspresi mRNA LMP2A EBV adalah 27,3% (6 dari 22 sampel). Kesimpulan, metode one step RT-PCR dapat digunakan untuk menganalisis ekspresi mRNA LMP2A EBV dari sampel biopsi jaringan KNF dalam blok paraffin dan hasil positivitas ekspresi mRNA LMP2A EBV sedang, sehingga berpotensi digunakan sebagai petanda biologi molekul diagnosis KNF