Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
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Analisis Molekuler dan Uji Ketahanan Tanaman Padi Transgenik yang mengandung Gen Kitinase Generasi ke tiga (T2) terhadap Rhizoctonia solani Kuhn dan Pyricularia oryzae Cav
Full text linkSheath blight and rice blast are two important diseases in rice caused respectively by fungi Rhizoctonia solani and Pyricularia oryzae resulting in considerable yield loss. A study on the segregation pattern of chiI gene among four progenies, i.e. lines 1,7,9 and 20, followed by bioassays of both pathogens was conducted. PCR analysis using specific primers for chiI gene in 30 plant samples from each line was employed to know the segregation pattern. The results showed that three of the four lines, i.e. 1, 7, and 9 performed Mendelian segregation of 3 : 1. Southern blot analysis further applied on line 1 revealed the presence of six copies of chiI gene. Bioassay of P. oryzae carried out at vegetative phase indicated that all lines were susceptible to blast, on the other hands, R. solani bioassay held at heading phase showed that they were all resistant to this fungi
Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus)
Full text linkA study on sequence variation in the mitochondrial DNA of Trochus niloticus has been conducted. Polymerase chain reactions (PCR) technique was applied to amplify cytochrome c oxidase I gene of 533 bp and ABI 310 aotomatic DNA sequencer was used to sequence the PCR product. This study was aimed to know the genetic variation of T. niloticus and the suitability of partial sequence of cytochrome c oxidase I gene as a molecular marker in the genetic study. The results showed that from two T. niloticus specimens, 12 polymorphic sites were detected yielding 2 haplotypes. This indicated a highly genetic variation in thecytochrome c oxidase I (COI) gene of T niloticus. In addition, cytochrome c oxidase I gene was proved to be a suitable marker for the genetics studies of various species of organisms
Kebiasaan Makanan Ikan Beloso (Saurida undosquamis) di Perairan Laut Cina Selatan Bagian Selatan (LCS)
Full text linkThe objective of this research is to understand the food habit of the lizard fish (Saurida undosquamis) in Southern South China Sea.For this purpose, purposive random sampling was conducted over 19 stations in trawlable bottom waters using swept area method and sample of 250 of S. undosquamis were examined for their stomach content. The lengths of S. undosquamis under this observation were 15,0-26,8 cm. The result showed that the S. undosquamis is demersal fish that distributed diurnally. The food habit of the S. undosquamis in South China Sea was different between the day and the night. The food habit of S. undosquamis in the day are mainly small demersal fishes, especially Leiognathus leusiscus and Upeneus sulphureus that have the same diurnal distribution pattern as S. undosquamis. Meanwhile, during the night time, the food habit of S. undosquamis was dominated by shrimps
Keanekaragaman Tumbuhan Pakan Bagi Tapir (Tapirus indicus), Kijang (Muntiacus muncak), Kukang (Nycticebus coucang) dan Kondisi Habitat di Kawasan Gunung Tujuh, Taman Nasional Kerinci Seblat, Jambi
Full text linkA study on the diversity of feed plants of tapir (Tapirus indicus), barking deer (Muntiacus muncak), and slow loris (Nycticebus coucang) as well as their habitat condition has been conducted at Gunung Tujuh, Kerinci Seblat National Park, Jambi. Survey was carried out by visiting places where the animals are usually found and by taking collection of herbarium plant specimens of those animals’ feed. The habitat of tapir is in a region of Gunung Tujuh forest up to the altitude of 2,020 m above sea level, while that of barking deer is in Gunung Tujuh forest where dense bushes at the edges of forest until the altitude of 1620 m above sea level is favored and that of slow loris is in low region of forest. Thirty eight species belonging to 25 families of forest plants as feed resources for tapir, barking deer, and slow loris were found. Amaranthaceae, Anonaceae, Clusiaceae, Moraceae, and Myrsinaceae were recorded as feed plants for tapir, while Euphorbiacea, Fagacea, Lauracea, and Urticacea were for slow loris and Solanaceae was for barking deer