Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
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Pengaruh Lama Inkubasi Soyghurt Menggunakan Inokulan dengan Penambahan Bifidobacterium sp. terhadap Daya Hambat Bacillus cereus
Get PDFBakteri asam laktat (BAL) yang digunakan dalam pembuatan soyghurt di antaranya Lactobacillus bulgaricus, Streptococcus thermophillus, dan Bifidobacterium sp. dengan suhu optimal pertumbuhan 36°-37°C dan lama inkubasi berkisar 1-2 hari. BAL diketahui mampu menghasilkan bakteriosin yang dapat menghambat bakteri patogen dalam saluran pencernaan salah satunya ialah Bacillus cereus. Tujuan penelitian ini ialah mengetahui perbandingan BAL pada soyghurt terhadap daya hambat B. cereus, mengetahui lama inkubasi soyghurt dengan penambahan Bifidobacterium sp terhadap pertumbuhan B. cereus, dan mengetahui pengaruh konsentrasi BAL dan lama inkubasi pada soyghurt terhadap pertumbuhan B. cereus. Hasil penelitian menunjukkan perbandingan BAL (L. bulgaricus : S. thermophillus : Bifidobacterium sp.) 1:1:1 (K1) pada soyghurt mampu menghambat B. cereus. Lama inkubasi soyghurt dengan penambahan Bifidobacterium sp. optimal pada 24 jam terhadap pertumbuhan B. cereus. Konsentrasi BAL dengan lama inkubasi pada soyghurt tidak mampu meningkatkan daya hambat pertumbuhan B.cereus
Faktor-Faktor Ekologis yang Berpengaruh terhadap Struktur Populasi Kumbang Badak (Oryctes rhinoceros L. )
Get PDFKumbang badak (Oryctes rhinoceros L.) dapat hidup dengan optimal dengan dukungan dari faktor ekologis yang cocok. Faktor ekologis meliputi habitat limbah material organik di vegetasi tersebut, musuh alami, musim, dan faktor cuaca (suhu, curah hujan dan kelembaban) diteliti pengaruhnya terhadap struktur populasi kumbang badak pada Oktober-Desember 2015. Penelitian ini merupakan penelitian survei dengan pengambilan sampel dilakukan sebanyak empat kali, dua kali pada musim kemarau dan dua kali pada musim penghujan. Data dianalisis dengan analisis ANOVA dan untuk mengetahui faktor ekologis yang paling berpengaruh dilakukan uji lanjut dengan Duncan. Berdasarkan ANOVA, ada perbedaan antar habitat F=0,012 untuk perbandingan antara jerami dan serasah daun, 0,002 antara jerami dan tanpa limbah, 0,000 antara serasah daun dan tanpa limbah, serta 0,001 untuk batang pohon dan tanpa limbah (P<0,05). Untuk stadium pupa dan imago hasil analisis ANOVA menunjukkan hasil tidak berbeda nyata. Faktor musim memberikan hasil bahwa terjadi peningkatan di ketiga stadium yang ditemukan dengan jumlah yang berbeda. Stadium larva mengalami peningkatan sebanyak 381 larva, stadium pupa sebanyak 25 pupa, dan peningkatan sebanyak 166 ekor kumbang badak stadium imago. Setelah dilakukan uji statistik dengan uji t, jumlah populasi kumbang badak pada musim kemarau dan penghujan berbeda nyata dengan nilai uji t =0,000 untuk stadium larva, sedangkan pada pupa, t = 0,038 (P<0,05). Setelah dilakukan uji lanjut diperoleh hasil habitat limbah material organik merupakan faktor ekologi yang paling berpengaruh. Habitat limbah material organik yang paling berpengaruh adalah batang pohon, disusul dengan habitat serasah dan jerami
PENGARUH INTENSITAS CAHAYA TERHADAP PERTUMBUHAN DAN KANDUNGAN KAROTENOID Dunaliella sp. PADA MEDIA EKSTRAK DAUN LAMTORO Leucaena leucocephala
Get PDFDunaliella sp. is a green microalga commonly used as a live feed in the hatchery, especially in marine aquaculture. This microalga is capable of producing carotenoids and accumulate a significant amount of β-carotene under stressful conditions, for example, high light intensity during the culture process. The objective of this study was to determine the effect of light intensity on the growth of Dunaliella sp. culture and it carotenoid content. The Dunaliella sp was cultivated for eight days at the Aquaculture Laboratory, Department of Fisheries and Marine Science, Faculty of Agriculture, University of Lampung. The study used a completely randomized design with three different light intensity as treatment, i.e., (A) 2500 lux, (B) 3500 lux, and (C) 4500 lux. All treatments were given three replicates, and the position of culture bottles was randomized with the assumption that all sample units received the equal amount of light intensity. The results showed the Dunaliella sp culture illuminated with light intensity higher than 2500 lux have higher carotenoid content
STRUKTUR KOMUNITAS DAN DISTRIBUSI TEMPORAL GASTROPODA DI DANAU SITU GINTUNG, TANGERANG SELATAN, BANTEN
Get PDFThe changes in the physicochemical of waters in the lake ecosystem caused by seasonal variation, anthropogenic and industrial wastes impact the gastropod community. This study aimed to determine the physicochemical parameter of waters, community structure and distribution of gastropod, and the correlation between those two in the dry and rainy seasons. We conducted this research in the lake of Situ Gintung located in Tangerang Selatan, Banten, Indonesia during the dry season (May to August 2015) and the rainy season (February to April 2016). Statistical analysis showed the physicochemical parameter of waters in both seasons has no different, while the gastropod showed a slight difference in occurrence based on the season, in which rainy season had the highest occurrence. Canonical Correspondence Analysis (CCA) suggested that gastropods occurrence were affected by the light intensity in the rainy season and the temperature in the dry season
Analisis Fenetik Kultivar Krisan (Chrysanthemum morifolium Ramat.)
Get PDFChrysanth (Chrysanthemum morifolium Ramat.) atau dikenal sebagai Seruni merupakan tanaman herba hias. Chrysanth merupakan salah satu komoditas tanaman hias utama yang memiliki keragaman kultivar, seperti dapat dilihat dari bentuk dan variasi warna bunganya. Penelitian ini bertujuan untuk menyelidiki keragaman dan hubungan kesamaan kultivar chrysanth. Metode penelitian yang digunakan purposive sampling. Data dari chrysanth kultivar morfologi dan anatomi daun karakter dianalisis berdasarkan hubungan kemiripan dengan UPGMA (Unweighted Pasangan Metode Group dengan Arithmetic mean) metode menggunakan 5.05 software MEGA. Berdasarkan penelitian, delapan kultivar chrysanth diperoleh, yaitu C. morifolium 'Cut Nyak Dien', C. morifolium 'Dewi Ratih', C. morifolium 'Dwina Kencana', C. morifolium 'Dwina Pelangi', C. morifolium 'Elora ', C. morifolium' Pasopati ', C. morifolium' Puspita Nusantara ', dan C. morifolium' Swarna Kencana '. Kesamaan terdekat adalah antara C. morifolium 'Dwina Kencana' dan C. morifolium 'Dwina Pelangi' kultivar, dengan indeks ketidaksamaan terkecil 0,152, sedangkan kesamaan paling jauh adalah antara C. morifolium 'Elora' dan C. morifolium 'Swarna Kencana 'kultivar, dengan indeks ketidaksamaan terbesar 2,256
COMPATIBILITY OF INSECTICIDES WITH ENTOMOPATHOGENIC FUNGI Beauveria bassiana AND Metarhizium anisopliae
No full textInsecticide use has produced negative impact by affecting the non-target predatory organisms in nature, one of which is the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae. Interactions occur, however, between insecticides and the entomopathogens. The combination of insecticides at a low dose and an entomopathogenic fungus can work synergistically to increase pest insect mortality. This combination is particularly advantageous because it decreases the insecticide dose applied, reduces environmental contamination, and decreases pest resistance. The study purpose was to determine the compatible working insecticide dose and the entomopathogenic fungi B. basiana and M. anisopliae. The experimental design applied completely randomized design consisting of 15 treatments and four replicates. There were five types of insecticides with three different doses each (0.5 × Dose of Field (DF), 1 × DF, and 2 × DF), whereas the fungal isolates included B. basiana and M. anisopliae. The parameters observed were the germination percentage of conidia, the percentage of inhibition, and the number of conidia.ml-1. Data were analyzed using ANOVA (5% error rate). The mean values were analyzed by DMRT p < 0.05. Deltamethrin 0.5 x DF, and imidacloprid 0.5 x DF demonstrated the highest conidial germination in B. basiana and M. anisopli, respectively. Insecticides showing the highest vegetative growth on B. basiana and M. anisopliae at 7 DAI was imidacloprid 0.5 x DF, while at 14 DAI was imidacloprid 0.5 x DF and chlorphyrifos 2 x DF, respectively. The highest conidial production of the fungi was triggered by imidacloprid 0.5 x DF. Based on compatibility calculation, imidacloprid 0,5 x DF worked with B. basiana (BI: 67.77) and M. anisopliae (BI: 67.16)
TINGKAT PENCEMARAN WADUK PENJALIN KECAMATAN PAGUYANGAN KABUPATEN BREBES DITINJAU DARI STRUKTUR KOMUNITAS PLANKTON
Get PDFPenjalin Reservoir is located in Brebes Regency, Indonesia. Human activity around Penjalin Reservoir, such as irrigation, aquaculture, and sanitation can affect its water’s quality. Plankton can be used as bioindicator since its existence is heavily affected by water physical and chemical factors. Polluted waters can affect diversity and abundance of plankton. The purpose of this research were to determine waters quality of Penjalin Reservoir, to know plankton community structure (abundance, diversity, and domination), and pollution level of each sampling station based on plankton diversity. This research used survey method on 6 sampling stations covering inlet, middle of reservoir, and outlet with 3 replicates and 4 weeks interval. Water quality were analyzed descriptively based on criteria of PP No. 82 Tahun 2001. Abundance and diversity were estimated as Shanon-Wiener index. Domination were estimated as Simpson index. Plankton abundance values varies from 4,970.30 to 12,923.37 ind/l with the most abundant species was Tetraedon minimum. Diversity index (H’) were high with value around 3.28–3.55 and domination index were around 0.04–0.07. These diversity indexes showed that the water quality of Penjalin Reservoir were not polluted
KERAGAMAN GENETIK 24 VARIETAS PADI SAWAH DAN PADI GOGO (Oryza sativa L.) INDONESIA BERDASARKAN MARKA SSR
Get PDFThe use of molecular marker is an efficient approach to analyze the genetic diversity and it can be used widely in biological studies. The characterization of rice germplasms by using molecular markers technique is more precise because it is not influenced by environmental factors. The purpose of this study was to analyze the genetic diversity of 24 varieties of lowland and upland rice by using 15 SSR markers. The results showed as many as 86 alleles were detected in 24 rice varieties, with the average number of alleles per marker was 5.73 and the range of alleles per locus was 2-10. The average of major allele frequency was 43% with the lowest score was 26% on RM6997 and RM536 markers and the highest score was 65% on RM60 marker. A total of 14 SSR markers were able to discriminate heterozygous alleles within a range between 0.17 (RM105) to 1.00 (RM201, RM263, RM416, RM518 and RM223). The value of gene diversity ranged from 0.48 (RM60) to 0.81 (RM536) with an average of 0.70. The value of PIC (Polymorphic Information Content) ranged from 0.38 (RM105) to 0.78 (RM536) with an average of 0.65. The phylogenetic analysis showed that 24 rice varieties separate into two main clusters in the coefficient of 0.63. The first cluster consists of 12 lowland varieties and the second cluster consists of 12 upland varieties. The genetic diversity data in this study were expected could be a valuable information in the rice plant breeding activities in the future
DIFERENSIAL LEUKOSIT IKAN GURAMI (Osphronemus gouramy Lac.) DENGAN PERBEDAAN LEVEL SUPLEMENTASI Spirulina platensis DALAM PAKAN
Get PDFThe aim of this research was to know the effect of supplemented Spirulina platensis in diet on leukocytes differential of gourami (Osphronemous gouramy Lac.) and to obtain the best dose. This research was carried out experimentally using a completely randomized design (RAL), which consists of 4 treatments and 3 repetitions. The fish fed with diets which contain different levels of (0, 2.4, and 6g/kg) Spirulina platensis were investigated. Sampling was done 4 times at intervals of 2 weeks of the feeding experiment, leukocyte differential (monocyte, lymphocyte, neutrophil, eosinophyl) of blood taken from fish were determined. The results showed that the highest percentage of lymphocytes was found in level of supplementation of 4 g/kg of diets, whereas the highest percentage of monocytes, neutrophils and eosinophils were found at the level suplemetation 2 g/kg of diets. Level supplementation of S. platensis 4 g/kg of diets is an efficient and effective level in stimulating immune system cells of gourami, particularly lymphocytes cell
