Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
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Karakterisasi Molekuler Ikan Gurami Soang (Osphronemus gouramy Lac.) yang Mati pada Rentang Waktu Berbeda Menggunakan PCR-RFLP Gen Major Histocompatibility Complex Kelas II B
Get PDFGurami (Osphronemus gouramy Lac.) is a popular fish species among Indonesian people. Several Gurami strains have been cultivated by fish farmer, one of which is Gurami Soang. This strain is belived to have a faster growth rate compared to other strains. However, like other strains, the fingerling of Soang strain have also a low survival and suceptible to disease, especially that caused by Aeromonas hydrophila infection. It has been proved that seeds from a single spawning event show varibale disease resistance. The difference in resistance among individuals is suggested related to the difference in their genetic component. One of the genes responsible for resistance is Major Histocompatibility Complex (MHC) class II B gene. Variability in resistance can be analyzed by using PCR - RFLP technique. PCR-RFLP is a technique that can produce a specific DNA fragments by PCR, followed by cutting the PCR product using restriction enzymes to describe the presence or absence of restriction sites in DNA fragments. This research aims to determine genetic marker to differiantiate between resitant and irresistant individual of Gurami Soang infected by A. hydrophila which die at a different time priod based on PCR-RFLP MHC class IIB gene. The study used survey method with purposive random sampling. The Data of PCR-RFLP band patterns were analyzed descriptively. The result indicated that cutting of the MHC class II B gene using HinfI produce two RFLP bands with 300 bp and 100 bp length in all samples. Meanwhile, the MHC IIB gene was not cuted by PstI, HindIII, BamHI and EcoRI enzymes forall samples. These mean that MHC II gene in all individuals were monomorphic. Therefore,it can be concluded that there is no specific genetic marker to differentiate gurami soang individulas which was dying in different time periods
Ekspresi mRNA BRLF1 Virus Epstein-Barr dari Biopsi Jaringan Tumor Formalin-Fixed Paraffin Embedeed sebagai Petanda Biologi Molekul Diagnosis Karsinoma Nasofaring
Get PDFNasopharyngeal carcinoma (NPC) is a malignant tumor that grows in the nasopharynx with a predilection in the fossa Rosenmuller. Epithelial malignancies are often found in populations of China and Southeast Asia, including Indonesia. The NPC incidence in year 2008 as many as 84,400 cases and 51,600 of these cases resulted in death. A total of 120 new cases per year NPC found in hospitals Prof. dr. Margono Soekarjo (RSMS), Purwokerto. The NPC is difficult to be diagnose caused its primary tumor lies closed to the skull base as well as various structures of vital organs. Therefore, methods that can detect early NPC required for inspection.The etiology of NPC is multifactorial consisting of genetic factors, factors of infection Epstein-Barr Virus (EBV) and environmental factors.EBV has two phases in the cycle of infection that is the phase of lytic and latent phase. BRLF1 has an important function as mediator transition from latent e NPC. The research aimed to analysis mRNA BRLF1 expression as a biomarker of NPC diagnosis by RT-PCR and to determine the positivity of RT-PCR method to detect the expression of mRNA BRLF1. The research design was cross sectional study. Samples were FFPE tumor biopsy of NPC WHO III and the total samples were 22 individu from Department of Pathology Anatomy, Prof. Dr. Margono SoekarjoHospital, Purwokerto with informed consent. The positivity of mRNA BRLF1 from FFPE tumor biopsy of NPC WHO III was in 63.6%indicating a high expression
Intensitas dan Variasi Morfometrik Trichodina sp. pada Benih Ikan Gurami (Osphronemus gouramy Lacepede) Pendederan I yang Dijual di Pasar Ikan Purwonegoro Kabupaten Banjarnegara
Get PDFPurwonegoro Fish Market, Banjarnegara district is the largest fish market in Central Java southern and western parts. This fish market serves as a center for buying and selling fish and fish seed traffic in a particular place to the location of the fish farmers. It will possibly spread a particular type of disease from one area to another. Trichodina sp. is found in the seed of gouramy fish hatchery center area with the pathogenicity level reached 80%. Trichodina sp. has a big role in the cultivation of gouramy production by reducing the physical condition of the fish so dangerous and cause death of gouramy in the seed phase. The results showed that as many as 150 gouramy fish fry were examined, 56 gouramy fish fry were infected Trichodina sp. All of Trichodina sp. are found as many as 573 individual from the gouramy seeds infected. Total intensity of Trichodina sp. from gouramy seeds amounted to 10.97 individual. The measurement results morphometric characters Trichodina sp. show the variation of morphometric with body diameter from 47.5 to 112.5 μm; denticle ring diameter from 22.5 to 47.5 μm; denticle diameter of 12.5 to 42.5 μm, adhesive disc diameter 37.5 to 65 μm; membrane width 2.5-7.5 μm and the number of denticle 13-28. Trichodina sp. allegedly found there are three types of T. heterodentata, T. nigra, and T. acuta
EFEKTIFITAS BAKTERI ENDOFIT DAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DALAM MENINGKATKAN PERTUMBUHAN TANAMAN PADI Oryza sativa L.
Get PDFRice plant needs essential and non-essential nutrients to grow. However, the use of inorganic fertilizers may affect the soil microbe community which expected to increase the availability of nutrients for both plants and microbes, a condition that could control several types of diseases. Many efforts were made to find alternative fertilizer which environmentally friendly, for instance by exploiting microorganisms such as endophytic bacteria associated with plant tissue or seed plant cell. This research aimed to determine the effect of endophytic bacteria, the concentration of IAA, and the combination of endophytic bacteria with a concentration of IAA, on the growth of rice plant. This research was conducted at the Laboratory of Soil Biology, The Soil Research Institute located in Cimanggu Bogor from December 2015 to February 2016. This research was a Completely Randomized Design (CRD) with two factors, i.e., the inoculant of endophytic bacteria (2.2 KT, KR 6, and I CM), and the concentration of IAA (0, 0.01, 0.1, 0.5, and 1 ppm). Three replicates were applied for each treatment of the inoculant combined with different doses of IAA. The variety of rice plants tested were Inpari 13 and IR 64. The potential test has been applied to determine the factor with the highest yield of the rice plant growth. The parameters observed were the height of the rice plant, the length of the rice plant roots, and the total weight of the plant including root. The results showed the inocula of endophytic bacteria alone had no effect to increase growth for both IR 64 and Inpari 13 rice variety. The IAA concentration of 0.1 ppm was able to increase the height of rice plant and the length of rice plant root for both IR 64 rice plant and Inpari 13. The combined treatment of KR 6 bacterial inoculum with 1 ppm of IAA concentration was effectively promoted rice plant growth for both IR 64 and Inpari 13
SPIDER DIVERSITY (ARACHNIDA: ARANEAE) OF THE TEA PLANTATION AT SERANG VILLAGE, KARANGREJA SUB-DISTRICT, DISTRICT OF PURBALINGGA
Get PDFSpiders are crucial in controlling insect pest population. The various cultivation managements such as fertilizer and pesticide application, weeding, pruning, harvesting, and cropping system affect their diversity. In the plantation, vegetation diversification has applied various practices, including monoculture, and intercropping, which influence the spider community. Thus, this study was intended to determine the spider abundance and diversity of the tea plantation, and the intercropping field (tea and strawberry) at Serang village, Karangreja Sub-District, District of Purbalingga. A survey and purposive sampling techniques were conducted, then the spiders were hand collected. Shannon-Wiener diversity (H’), Evenness (E), Simpson’s dominance (D), and Sorensen’s similarity (IS) indices were used to measure the spider diversity. The results revealed a total number of 575 individual spiders from 10 families, i.e., Araneae, Araneidae, Clubionidae, Linyphiidae, Lycosidae, Nephilidae, Oxyopidae, Salticidae, Tetragnathidae, Theridiidae, and Thomisidae. Araneidae was the most abundant in both fields. The total abundance of spiders in tea plantation (379 individuals), however, was greater than that in the intercropping field (196 individuals). Shannon-Wiener diversity reached H’= 1.873 in the plantation, and H’= 1.975 in the intercropping field
ISOLASI DAN KARAKTERISASI JAMUR LIGNINOLITIK SERTA PERBANDINGAN KEMAMPUANNYA DALAM BIODELIGNIFIKASI
Get PDFFungi can survive in various environments with different media including wood. Lignin in timber is hard to be degraded and hydrolyzed efficiently because of its polymer form, composite and complex structure. Ligninolytic fungi produce an extracellular enzyme to withstand with toxic or mutagenic chemicals exposure and known to degrade different types of pollutant compounds. Lignin decomposers were also known to play a significant role in the pulping process of paper mills, used in waste treatment such as textile and hydrocarbon wastes. This study was conducted to obtain fungal isolates that have delignification capability and to compare the ability of fungal isolates in degrading lignin. Isolates were from rotten wood and soil using selective lignin medium with tannic acid as sole C source. This study characterized the isolates by their morphology and identified them using Morphology and Taxonomy of Fungi book by Bessey (1950). The ligninolytic capability comparison was conducted by measuring the transparent zone formed on selective lignin media. This research found 14 isolates of fungi and all of them had the ligninolytic capability. Aspergillus niger isolate has the highest ligninolytic capability by producing 6.45 cm clear zone diameter on the 7th day of incubation. Aureobasidium sp. has the smallest clear zone diameter of 1.9 cm within the same period
ANALISIS BIOMASA DAN CADANGAN KARBON PADA BERBAGAI UMUR TEGAKAN DAMAR (Agathis dammara (Lamb.) Rich.) DI KPH BANYUMAS TIMUR
Get PDFIncreased carbon dioxide in the atmosphere causes global climate change seriously. Forests serve as an important asset that can absorb and store carbon in the form of biomass. One type of potential forest as a carbon sink is forests resin. The amount of carbon stored by standing very dynamic and varies according to the age of its standing. Therefore, studies will be needed to determine the effect of age on biomass and carbon stocks stands resin, determine the relationship between age and standing biomass and carbon stocks resin, and knowing the optimum resin stand age in storing biomass and carbon stocks. This research was conducted at the stands of resin RPH Karang Gandul, KPH Banyumas Timur for four weeks in May 2016. The method used is a survey with a sampling technique using cluster random sampling. Stands resin used in the study were classified into five age groups with 5 replicates. Data were analyzed using ANOVA with an error rate of 5% and continued with LSD for further test results were significant and regression analysis to determine the relationship of age with biomass and carbon stocks stands resin. The results showed that the age effect on biomass and carbon stocks stands resin, the relationship formed between the age of stand with biomass and carbon stocks are quadratic, and age optimum in storing biomass and carbon stocks is 35 years
PENAMBAHAN EKSTRAK KULIT BUAH NAGA KE DALAM PENGENCER TRIS KUNING TELUR UNTUK MENINGKATKAN KUALITAS SEMEN BEKU KAMBING PERANAKAN ETTAWA
Get PDFThe success of artificial insemination is determined by the quality of the frozen semen used. Free radicals and cold shock may reduce the quality of frozen semen. These can be prevented or reduced by the addition of antioxidants. Dragon fruit skin extract is rich in natural antioxidants. This study is aimed to analyze the effect of the addition of dragon fruit skin extract on quality (motility, viability, and abnormality) of frozen goat semen. The most effective concentration in frozen semen. The true experimental research was used with the posttest-only control group design. Data analysis was done by using One Way ANOVA followed by Duncan's test of 0.5% assisted by SPSS 22. The research was conducted in May–June 2017 at BBIB Singosari, Malang. The result showed that 0.50% concentration gave the highest motility (26.00 ± 6.51%) and the lowest abnormality (5.6 ± 2.7%), while at 0.75% gave the highest viability (61.60 ± 8.11%). It can be concluded that there is a significant effect (p < 0.05) addition of dragon fruit’s rind extract to the frozen semen quality (motility, viability, and abnormality) of ettawa goat and the most effective extract concentration varies on each quality parameter
Uji Antagonisme Lentinus cladopus LC4 terhadap Ganoderma boninense Penyebab Penyakit Busuk Pangkal Batang Kelapa Sawit
Get PDFThe growth of oil palm can not be separated from disease attack. One of the diseases that attack is the Base Stem Rot disease caused by Ganoderma boninense. Efforts to control the pest and disease one of them by using Lentinus Cladopus LC4 biological controller so that the purpose of this study is to determine the potential of L.cladopus LC4 as biological controlling agent G. boninense. The method used is L. cladopus LC4 antagonism test against G. boninense. The results showed that L. cladopus LC4 had antagonistic potency against G. boninense pathogen, although the mechanism did not show any inhibition zone. Therefore, L.cladopus LC4 may be considered for the prevention and control of plant diseases in the field