Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
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Konservasi Eksitu Jenis Begonia Alam Pegunungan Sumatra di Kebun Raya Cibodas
Full text linkKegiatan konservasi secara eksitu Begonia alam dari pegunungan telah dilakukan di Kebun Raya Cibodas sejak tahun 1930. Keanekaragaman jenis dan persebaran Begonia Sumatra didata berdasarkan koleksi Kebun Raya Cibodas, ditambah dengan spesimen herbarium (Universitas Andalas (ANDA) dan Cianjur Hortus Tjibodasensis). Informasi perbanyakan, hama dan penyakit tanaman didata selama kurun waktu dua tahun (2015-2017). Lima belas Begonia alam Sumatra telah dikonservasi di Kebun Raya Cibodas. Secara umum, Begonia dapat diperbanyak secara vegetatif maupun generatif. Perbanyakan secara in vitro dilakukan pada B. leuserensis, B. atricha dan B. scottii, jenis begonia endemik dan langka di Sumatra, telah dilakukan untuk meningkatkan jumlah begonia tersebut dalam botol kultur. Permasalahan konservasi Begonia di Kebun Raya Cibodas didiskusikan secara singkat
Keragaman Bakteri pada Perairan Sabang, Provinsi Aceh
Full text linkSabang seawater have high diversity and its geographical condition allow hot springs to form, therefore, current research on this seawaters become important. The purpose of this study was to discover the quality and diversity of the seawaters based on microbiological aspects specifically the finding of coliform, heterotrophic, halotolerant and sulfur bacteria from several samples from the water and sediment sampling. Analysis and isolation process of the bacteria were using coliform and aerobic count plate petrifilm and specific media. The results indicated that there were coliform bacteria, heterotrophic and halotolerant bacteria and several of hot spring sources under the sea surface with extremely high density of sulfur bacteria as geothermal microbe
KEANEKARAGAMAN UDANG AIR TAWAR (DECAPODA: CARIDEA) DI SUNGAI BATUSUYA, SULAWESI TENGAH, INDONESIA
Full text linkSulawesi is one of the four Greater Sunda Islands characterized by rivers and lakes habitats of freshwater shrimps and prawns with a high degree of endemism. The freshwater shrimp and prawns diversity in the Batusuya river of Donggala is less studied compared to the ancient lakes in the Central Sulawesi area. This study aimed to determine the diversity of freshwater shrimp and prawns of the Batusuya river. The sample was collected in November 2016 using tray net. The study site divided into three sampling stations based on the type of habitat. Nine species of freshwater shrimps and prawns were found, i.e., Macrobrachium australe, M. esculentum, M. horstii, M. lar, M. placidulum, Caridina brevicarpalis, C. gracilipes, C. weberi, and Atyopsis spinipes. Six species were found in all localities including Macrobrachium australe, M. esculentum, M. horstii, M. lar, C. brevicarpalis, and C. gracilipes, while M. placidulum, C. weberi, and A. spinipes were not found in all stations. The highest diversity index (H') was found in the station III indicated freshwater shrimps and prawns favored the environmental conditions of this station. The highest measure of species richness (R) was found in the station I and presumably related to the life cycle of freshwater shrimps and prawns which is amphidromous. The occurrence of nine species of freshwater shrimps and prawns along the Batusuya river indicated the environmental condition of the stream was suitable for most freshwater shrimps and prawns, and the Macrobrachium australe was found as the most abundant species
KEANEKARAGAMAN LICHEN SEBAGAI BIOMONITORING KUALITAS HUTAN DI LERENG SELATAN GUNUNG MERAPI YOGYAKARTA
No full textLichen is an symbiotic organism between algae (Photobiont) and Fungi (Mycobiont). Eruption of Merapi vulcano can be affected lichen diversity. In this research involved a method of descriptive explorative and the aim of this research is to study the diversity of lichen, its character, its distribution and its secondary metabolite in the forest of Turgo dan Kaliurang. Sampling used cruise method. This research based on morphological, chemical test, and Pb and S of metal test. The research was conducted from April to November 2017. The result of research was found three family, there are is Parmeliaceae, Physciaceae, and Lobariaceae. The result of research in Turgo forest found 4 species, there are is Usnea bismolliuscula, U. baileyi, Parmelia sp., and Parmelia crinita, in Kaliurang forest was found 4 species, there are is Parmelia crinita, Parmelia perlata, Physcia sp. and Lobaria amplissima. The result of metal test in Parmelia perlata and Lobaria amplissima was detected sulfur and undetected of Pb metal. The forest of Turgo and Kaliurang was included healthy forest. Lichen is recommended as healthy bioindicator and biomonitoring in forest
Keanekaragaman dan Potensi Lobster (Malacostraca: Palinuridae) di Pantai Pameungpeuk, Garut Selatan, Jawa Barat
Full text linkCoral shrimp (Panulirus spp.) or known as lobster is a potential fishery commodity and economic value. The demand for domestic and export market continues to increase both as local consumption and export. Indonesia is the largest lobster fishery producer in almost all Indonesian waters, from the west coast of Sumatra to the east coast Jayapura, one of which is Pameungpeuk beach, South Garut, West Java. As a result of increasing demand, fishermen try to catch as many lobsters and the impact of lobster prices also in creases and whereas fishermen continuously take it from nature. This study was conducted to determine the diversity and potential of lobster species especially living in high wavy waters along the southern coast of Garut (Pamengpeuk), West Java.The results of observation are only three types of crayfish, namely: a. Panulirus homarus (green lobster sand); b. Panulirus longipes (lobster flower/ red lobster) and Panulirus versicolor (green lobster/bamboo); is most commonly found in the area of south Garut, Pameungpeuk beach with high and strong waves. The percentage of female P longipes species (42.88%) was higher compared to male P. homarus (40%) and ovigerous female of P. versicolor (37.14%). While the sexual ratio between P. homarus (1:2.0:2.0); P. longipes (1:1.2:1.0) and P. versicolor: 1:1.2:1.2 which statistically showed significantly different p <0.05
AKTIVITAS TRIPSIN-LIKE DAN KIMOTRIPSIN-LIKE PADA IKAN SIDAT TROPIK Anguilla bicolor McClelland
Full text linkThe ability of eel (Anguilla bicolor McClelland) to digest feed protein is highly dependent on the alkaline proteases in the intestine which are trypsin-like and chymotrypsin-like. Information on the trypsin-like and chymotrypsin-like activities is needed to understand the digestive capacity of eels to ingest feed proteins. However, the information on this subject is insufficient. This research aimed to determine the activity of trypsin-like and chymotrypsin-like of eel on the different body weight and incubation temperature. Three groups of eel stadia were selected to represent different body weight, i.e., elver, yellow eel, and pre-silver stadia, and three different enzyme incubation temperatures of 30, 40, and 50°C was applied. The trypsin-like and chymotrypsin-like activity were measured using a spectrophotometry method. The results showed the activities of trypsin-like and chymotrypsin-like were significantly different between the body size and the intestine segment (P 0.05). This study showed the eel had a higher digestive capacity of protein at elver stadium, mainly occur in the anterior part of the intestine, whereas, the temperature ranges from 30 to 50°C had no significant effect on the enzyme activities
BIODEGRADATION OF DIESEL OIL BY YEAST ISOLATED FROM MANGROVE’S RHIZOSPHERE
Full text linkDiesel oil-degrading yeast strains isolated from mangrove rhizosphere at Tritih Kulon, Cilacap had been screened with SMSS medium. Four culturable yeast were isolated. Qualitative test was conducted by culturing and incubating the yeasts for one month in a medium added with 1mL diesel oil. By measuring the reduction of diesel oil, two best yeasts were selected. The quantitative test, GC-MS analysis, was conducted to determine the detailed degradation process of diesel oil. Candida lusitaniae and Cryptococcus laurentii performed the degradative ability. Three highest percent area of hydrocarbon compounds were compared for assessment. The results showed that C. lusitanie had better degradative capability than C. laurentii, in which hexadecane and methyl hexadecanoate decreased by 90–95%, and 9-octadecenoic acid, methyl ester declined by 30–40%. The increasing pH medium during incubation suggested that fermentation process occurred
A CHECKLIST OF FREE-LIVING MARINE NEMATODES AT DIFFERENT ECOSYSTEM IN NORTHERN STRAITS OF MALACCA, MALAYSIA
Full text linkNematodes present in all substrates and sediment types with an enormous number of species. The studies on free-living marine nematodes are still limited in Malaysia. The present study provides general information on genus checklist of free-living marine nematodes in the northern part of Peninsular Malaysia in a different ecosystem which are intertidal zones of estuarine, sandy beach and mangrove ecosystems. Samples were randomly collected at three stations in each ecosystem. A total of 34 genera (22 families and ten suborders) were recorded in three ecosystems. Each ecosystem had common genera which also commonly found worldwide. Viscosia genus was observed in all three ecosystems. The nematode genera of Estuary and mangrove ecosystems had greater similarities than those in the sandy beach
CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM Solanum tuberosum L. var. Granola POTATO
Full text linkPotato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe
Pertumbuhan Stek Krisan (Chrysanthemum morifolium (L.) Ramat) pada Berbagai Media Kultur In Vitro
Full text linkChrysanthemum (Chrysanthemum morifolium (L.) Ramat is one of ornamental plant commodities with high aesthetic value. As a consequence of increasing demand, the availability of seeds in adequate number is needed with method in vitro. This study aims to find out the effect of various in vitro culture media on the growth of chrysanthemum explants and to determine alternative media other than MS for in vitro growth of chrysanthemum explants. Experimental method arranged in a Randomized Complete Block Design (RCBD) with four treatments and four blocks is applied. The treatments are Murashige and Skoog (MS), Vacint and Went (V&W), Knudson and modified media, while node position serves as block. Time of shoot emergence, shoot length, number of leaves on each shoot, and number of roots are measured. Data are analyzed using Analysis of Variance (F test) with confidence intervals of 95% and 99%. Least Significant Difference (LSD) test is applied when significant difference among variables is observed. Culture media shows significant effect on chrysanthemum explant in vitro growth. Knudson media is found as an alternative media besides MS sufficiently suitable to increase chrysanthemum explants in vitro growth