Publikasi Online Fakultas Biologi UNSOED (Universitas Jenderal Soedirman)
Not a member yet
384 research outputs found
Sort by
FAKTOR RESIKO GANGGUAN FUNGSI GINJAL PADA PEKERJA BENGKEL LAS DI KOTA PURWOKERTO
No full textWelding was one of the industry in Purwokerto City. One of the exhaust particulate material from the welding process, this will be the potential occurrence of exposure to workers and caused renal dysfunction. Several risk factors have contributed in caused kidney difunction of workers. This study aims to determine the levels of cadmium metal (Cd), chromium (Cr), lead (Pb) with creatinine and Glomerulus Filtration Rate (GFR). The correlation of risk factors for renal dysfunction with creatinine and GFR. The risk factors that play a major role in caused kidney dysfunction. The method used is cross sectional. Sampling using purposive sampling with giving inform consent to the respondent. Provision of questionnaires and blood sampling as much as 3 ml according to ethical clearens. Measurement of metal content of Cd, Cr and Pb; serum creatinine and LFG levels. Number of respondents 30 people with 95% confidence level. The results showed that levels of Cr, Cd and Pb were 0.049 + 0.12 ppm; 1,029 + 2.38 ppm; 4,933+ 11,66 ppm. Cr levels are still within normal limits with levels but Cd and Pb exceeding the normal threshold. Creatinine levels of 1.867 + 0.446 mg / dl showed higher than normal. Minimum of GFR decrease in welding workers was 44 mL / min / 1.73 m2. Risk factors significantly correlated with creatinine and GFR was levels of Pb (p <0.05). Risk factors that play a major role in caused kidney dysfunction in welding workers in Purwokerto city is Pb content with 12.9% percentage effect on serum creatinine level and 9% effect on Glomerulus Filtration Rate
PENGGUNAAN PESTISIDA DAN IMPLIKASINYA BAGI KESEHATAN PETANI DI KABUPATEN TIMOR TENGAH SELATAN, NUSA TENGGARA TIMUR
No full textPesticides pose a risk to public health and environmental in agricultural areas. This study described pesticide use among farmers in Oebobo, Batnun, Tupan and Pollo villages, South Central Timor, Indonesia. Some 400 farmers were interviewed about pesticide use, knowledge and attitude, symptoms of intoxication, personal protective equipment and hygiene. The farmers had adequate knowledge about pesticide toxicity, but farmer’s awareness did not necessarily translate into action. Majority did not use appropiate personal protective equipment nor good hygiene when handling pesticides. Farmers in South Central Timor did not use the most hazardous pesticides (class Ia and Ib). However, use of class II pesticides and those of lower toxicity in combination with inadequate practice among farmers was found in the study areas. Expensive protective equipment and behavioral patterns, such as spraying without protective clothing and mixing pesticides with bare hands combine to produce several well-documented health problems. Therefore, alternative crop management systems which could help preventing the negative effects of pesticides on farmer health and the environment are needed. Training farmers in integrated pest management methods, use of proper hygiene and personal protective equipment when handling pesticides should be promoted
Aktivitas Antioksidan Ekstrak Tubuh Buah Jamur Paha Ayam (Coprinus comatus) dengan Pelarut Berbeda
Full text linkCoprinus comatus or with local name is drum stick chiken mushroom that includes edible and medicinal mushroom. This mushroom contains bioactive compounds that have several pharmacological effects, like as antibacterial, anticancer, immunomodulator, anti-inflammatory, prevent diabetes and antioxidant. bioactive compounds includes in fruit body C. comatus can be obtained by extraction used solvent. The objective of this study was to determine whether the C. comatus fungus was potential as an antioxidant producer and to know which solvent produced the best antioxidant activity from the extract of the fruit body of C. comatus. The method used in this research is experimental with two treatments ie n-hexane and ethanol 70% solvent. Fruit body of C. comatus is extracted by maceration method, then the viscous extract obtained by phytochemical test and antioxidant activity test using DPPH method (1,1-diphenyl-2-picrihidazil). The results obtained are then analyzed using t-test for independent samples. The results showed that the average yield of ethanol extract 70% higher than n-heksan were each 61.33% ± 9,21 and 14.8% ± 0,87. The test of total phenol extract ethanol was 70% higher than n-hexane , each with an average of 325,19 ppm ± 50,01 and 110,08 ppm ± 34,67. The result of antioxidant activity test using DPPH method of ethanol extract 70% and n-heksan has IC50 value 2,48 mg/ml and 3,86 mg/ml. Phytochemical test results for flavonoid test showed positive result for both samples extract, terpenoid test showed positive result in both samples , the tannin test showed negative results in both samples
Aktivitas Antioksidan Ekstrak Fenol Daun Gayam (Inocarpus fagiferus Fosb)
Full text linkThis study aims to determine the total phenolic content and antioxidant activity in gayam leaf extract (Incarpus fagiferus Fobs). The research method used is a quasi-experiment that aims to predict the situation to be achieved through actual experiments but no treatment. The sample used is old gayam leaves, with the characteristic of dark green leaf and rough leaf surface. The process of preparing simplicia, ie preparing fresh gayam leaves, dried in an oven temperature 45-50oC, and then dried to produce gayam leaf powder. Samples were extracted with methanol solvent and ethanol for 5 days. The total phenol assay method uses Folin-Ciocalteau method and antioxidant activity test using DPPH free radical retardation method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the total phenolic content of gayam leaf extract with ethanol and methanol solvent was 313,704 GAE (Gallic Acid Equivalent) and 273,913 GAE, respectively. Antioxidant activity as a free antidote to free radical DPPH is known to be valued with IC50 (inhibitory concentration)
KERAGAMAN GENETIK VARIETAS PADI japonica DAN indica BERDASARKAN MARKA DNA TERKAIT MUTU RASA
Full text linkPCR-based markers and evaluation of physicochemical properties should be addressed for the improvement of rice varieties with good eating dan eating quality (EQ). This study aimed to examine the genetic diversity of rice varieties based on DNA markers related to physicochemical properties determining EQ. A total of 46 rice varieties consisting of 22 japonica varieties and 24 indica varieties were examined using 43 PCR-based markers. The results showed that polymorphic information content (PIC) ranged from 0.04 to 0.38, in support of genetic diversity indices which ranged from 0.04 to 0.50 across total markers. Pairwise genetic similarity matrix ranged from 0.40 to 0.98 with the closest genetic distance was observed between two japonica varieties (Dongjin and Hwaseong) and the most distant one was between japonica and indica (Onnuri/Manmi with Cigeulis/Fatmawati). The unweighted neighbor-joining tree clustered the rice varieties into two major clades, indica and japonica, and subsequent subclades were differentiating according to the individual genetic background. The genetic diversity of rice from different subspecies and DNA markers for EQ can effectively be utilized for basic information and marker-assisted selection (MAS) for the development of improved varieties with good EQ in rice breeding program
APPLICATION OF PROTEASE ENZYMES IN PROTEIN UTILIZATION AT GROWTH OF WHITE SNAPPER FISH (Lates calcalifer)
No full textWhite Kakap (Lates calcalifer) is a superior commodity and has a high nutritional value. Growth factors are hampered by the ineffectiveness of the digestive process. Protease is an enzyme capable of hydrolyzing peptide bonds in proteins. Feed is a factor that predominantly affects the growth of fish because feed serves as a supplier of energy to support growth. Protein digestion in aquatic animals requires proteases as a catalyst. Enzymes that play an important role in the process of protein digestion are proteases. The purpose of this study is to examine the effect of adding different protease enzymes to artificial feed on the efficiency of protein utilization in the growth of white snapper (Lates calcalifer). The results of this study are expected to provide information about the importance of protease enzyme application in artificial feed in increasing protein hydrolysis, so that protein can be easily utilized by the body of white snapper (Lates calcalifer). The cultivars tested were white snapper (Lates calcalifer). The cultivation comes from intensively cultivated Lampung with an average weight of 6.5 ± 0.44 g / tail with a length ranging from 4-5 cm, as many as 75 heads. The research method is experimental laboratories using Completely Randomized Design (RAL). The tested feed was in the form of artificial feed with protein content of 40% and different protease dosage. The tested treatment was A feed (0.0% Protease enzyme from artificial feed weight), B (0.05% Protease enzyme from artificial feed weight), C (0.1% Protease enzyme from artificial feed weight), D (0 , 15% Protease enzyme from artificial feed weight), E (0.2% Protease enzyme from artificial feed weight). The ingredients of white snapper fish feed consist of fish meal, corn flour, soybean meal, shrimp flour, fish oil, corn oil, CMC / wheat flour, minerals and vitamins. Research data obtained for 40 days using analysis of variance (anova). If there is a real effect on the treatment then Duncan multiple area test is done. The result of this research indicated that the giving of protease 0,2% in artificial feed with protein 40% able to produce feed consumed Kakap Putih 43,43 ± 0,13 g, FCR 0,35 ± 0,002, Feed Pureness equal to 67,9 ± 1.23%, Protein Digestibility 98.25 ± 0.03%, Protein Retention 68.8 ± 0.23%, PER 7.97 ± 0.04, Fat Retention 0.6 ± 0.005%, E / P 4, 6 cal / g, thus giving effect on Specific Growth (SGR) 6.032 ± 0.17% and Length 8.3 ± 0.2 cm
KONDISI FITOPLANKTON DAN KUALITAS AIR PADA KEDALAMAN PERAIRAN BERBEDA DI PERAIRAN MALUKU
No full textFitoplankton di laut berperan sebagai pembentuk dasar dari rantai makanan melalui produksi primer. Keberadaan fitoplankton sangat terkait dengan faktor fisika kimia perairan. Umumnya kondisi lingkungan perairan memiliki perbedaan konsentrasi di wilayah permukaan dan di perairan yang lebih dalam. Penelitian ini bertujuan untuk mengetahui dinamika planktonik di sekitar wilayah penelitian meliputi kelimpahan, dan komposisi fitoplankton serta kondisi fisika kimia perairan di wilayah perairan permukaan dan termoklin. Penelitian dilakukan pada bulan Oktober 2015, pada delapam lokasi penelitian. Sampling plankton dilakukan pada 2 kolom air yang berbeda, yaitu di kedalaman permukaan dan pada lapisan termoklin. Air sampel diambil dengan menggunakan Rosette sampler, kemudian disaring dengan menggunakan hand plankton net ukuran mata jaring 20 µm. Filtrat yang terkumpul di bucket lalu dimasukkan ke dalam botol sampel 250 ml dan difiksasi dengan formaldehyde 4%. Hasil penelitian menunjukkan bahwa di perairan Maluku ditemukan Bacillariophyceae (14 marga) dan Dinophyceae (6 marga). Terdapat kondisi parameter perairan yang berbeda pada kedalaman yang berbeda, khususnya untuk nitrat, fosfat, silikat, oksigen terlarut (DO) dan pH. Nitrat, fosfat dan silikat memiliki nilai-nilai yang lebih tinggi pada kedalaman termoklin dibandingkan di perairan permukaan, sedangkan DO dan pH memiliki nilai yang lebih tinggi pada perairan permukaan dibandingkan kedalaman termoklin. Kata Kunci: Fitoplankton, kualitas air, permukaan perairan, kedalaman termoklin, perairan Maluku
Penambahan Ekstrak Alga Sargassum duplicatum Bory pada Medium Kultur In Vitro terhadap Pertumbuhan seedling Anggrek Vanda tricolor Lindl.
Full text linkVanda tricolor is an orchid species that has many advantages, some of which are due to its beautiful and various flowers of unique shape and size. Propagation of V. tricolor can be performed by in vitro culture.In vitro culture technique needs appropriate medium to support orchid growth. An algae species, Sargassum duplicatum, can be added into the medium, since it contains growth regulating substances, such as auxin and gibberellin, which are useful for seedling growth. This study aims to know the effect of S. duplicatum application into in vitro culture medium of V. tricolor on the seedling growth and to know the best application of the algaein increasing seedling growth. An experimental method was arranged in a Completely Randomized Design (CRD). The treatments were S. duplicatum weight of 0, 12, 24, 36, 48 and 60 g/L replicated three times respectively. The variable examined was seedling growth with parameters comprising day of new leaf emergence, leaf number, leaf length, day of new root emergence, root number, root length and plant height. Data were analyzed using ANOVA or F test with confidence level of 95% and 99%. LSD was carried out when F test showed significant difference. The results reveal that application of S. duplicatum extract has significant effect on the growth of V. tricolor seedling. The best application is of 36 g/L algae weight
HUBUNGAN ANTARA KADAR UREUM, KREATININ DAN KLIRENS KREATININ DENGAN PROTEINURIA PADA PENDERITA DIABETES MELLITUS
No full textPemeriksaan ureum, kreatinin dan klirens kreatinin adalah pemeriksaan untuk monitoring fungsi ginjal seseorang. Pemeriksaan ini dapat dilakukan pada laboratorium pratama dengan akses yang terjangkau. Pemeriksaan albumin urin sebagai petanda dini dari komplikasi mikrovaskuler pada DM. albuminuria relative mahal dan tidak semua laboratorium dapat melaksanakan pemeriksaan ini, sehingga penelitian ini diharapkan dapat memberikan alternatif deteksi komplikasi nefropati diabetikum pada penderita DM. Membuktikan adanya hubungan ureum, kreatinin dan klirens kreatinin dengan proteinuria pada penderita DM. Harapannya pemeriksaan fungsi ginjal dapat dilaksanakan rutin pada pusat pelayanan kesehatan 1 untuk mendeteksi dini komplikasi nefropati pada penderita Diabetes Mellitus. Desain observasional analitik dengan pendekatan belah lintang (cross sectional). Responden 35 orang DM dilakukan pemeriksaan ureum dan kreatinin metoda enzimatik, Klirens kreatinin dengan perhitungan cockroft gault formula dan proteinuria dengan pemeriksaan albumin urin metoda immuno chromatografi. Hubungan antar variabel dianalisis dengan uji korelasi Pearson. Terdapat hubungan antara kadar ureum, kreatinin, klirens kreatinin dengan proteinuria (p=0,298;0,386;0,382).Komplikasi DM mikrovaskuler yang terjadi akibat kerusakan glomerulus menyebabkan sejumlah protein darah diekskresikan ke dalam urin secara abnormal. Protein utama yang diekskresikan adalah albumin. Peningkatan kadar albumin dalam urin merupakan tanda awal adanya kerusakan ginjal oleh karena diabetes. Terdapat hubungan sinifikan antara ureum, kreatinin dan klirens kreatinin dengan proteinuria pada pasien Diabetes Mellitus
STUDI PENYEBARAN SPASIAL PALEM DI HUTAN LINDUNG GUNUNG SLAMET, JAWA TENGAH [Study on Palm spatial distribution at Gunung Slamet Nature Forest, Central Java]
No full textEcological studies and the spatial distribution of two forest ecosystem, natural forest and mixed forest, on Mount Slamet, Baturaden, Central Java has been done. There are 12 species of palms in natural forests, Calamus asperimus, C. heteroideus, C. javensis, Calamus sp. 1, Calamus sp. 2, Caryota maxima, C. mitis, Daemonorops rubra, Pinanga coronata, P. javana, Plectocomia elongata, and Salacca zalacca. While in the mixed forest were found six species of palm that were C. asperimus, C. ciliaris, Caryota maxima, Pinanga coronata, P. javana, and Plectocomia elongata. The species that had highest Importance Value Index (IVI) in the two types of ecosystems were Pinanga coronata. Pattern of its distribution both in natural and mixed forests have Morisita index value> 0. This value means that palm has a clustered distribution pattern. The highest spatial distribution of natural palm forest was found in 8-15% slopee class and height class 1,100 - 1200 m asl. Whereas for mixed forest it is found mostly in slopee class 8-15% and height class 900 - 1,000 m asl