Pelita Perkebunan (Coffee and Cocoa Research Journal, CCRJ)
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Implications of Cocoa Pod Storage and Temperature to the Performance of Pod Components and Seedling Growth
Full text linkCocoa seeds are recalcitrant with some problems in their storage due to lack of dormancy period. This study aimed to find out the effect of cocoa pod storage in two different temperatures and the storage period towards seed viability and growth of the seedlings. This study applied a split-plot design with storage temperature as main plot and storage period as sub-plot. Cocoa pods of Sulawesi-1 clone were harvested and stored at 20ºC and 29ºC for 3, 7, 14, and 30 days. The stored cocoa seeds were then sown for three months. The findings showed that the storage of cocoa pods at 20ºC for 30 days causing the seed water content was 45%, the growth of the seedlings after the storage was 98.7%, and could press the pod shrinkage (1.6%). The storage of cocoa pods at 29ºC for 30 days could accelerate the reduction process by 68.2%, decreased germination of seeds upto 83.4%, and reduced the water content by 44.2%. The pod storage at 20ºC for 30 days showed a high value in some aspects such as seedling height, stem diameter, leaf area, seedling fresh weight, and shoot/root ratio. The storage of pods at 29ºC for 14 days indicated a high score in the character of germination, seedlingheight, stem diameter, and fresh weight of seedlings. The storage of pods at room temperature could be conducted for 14 days, meanwhile, at controlled temperature, it could be conducted for 30 days
Determination of Cocoa Powder Quality Produced by Small-Medium Enterprise Using Six Sigma Analysis
Full text linkCocoa powder now has become a common craft product of small-medium processing enterprises (SME) in Indonesia. Small-medium enterprises developprocessing sequence in smaller quantity compared to big company, nonetheless it must follow the national cocoa powder standard of SNI 3737:2009. The aim ofthis experiment was to evaluate the SME cocoa process production performance through Six Sigma analysis of the product to comply the standard requirements.The cocoa powder product was evaluated using three critical to quality (CTQ) parameters involving water content, color, and fineness. National standard determinedthe maximum water content value is 5%, the color is brown or Agtron number 65 and minimum fineness percentage of particle (75 µm) must be 99.5%. This resultexplain the stability process of SME which is exceeding the control limit value of non conformity product. Its process capability showing the Sigma value of2.6 with defect per million value was 126,667. The CTQ parameters which contribute to the high non-conformity are fineness 78.95% and color 21.05%. The roughcocoa powder or fineness affects the highest non-conformity related to the high fat content in cocoa cake as input process and can be revised by upgrading thepressing machine performance. 
Callogenesis and Embryogenic Potential of New Superior Cocoa (Theobroma cacao L.) Genotypes Treated with Ascorbic Acid
Full text linkClonal propagation is an alternative method of maintaining the genetic purity of superior cocoa plants (Theobroma cacao L.). The somatic embryogenesis technique is likely the most effective and efficient method for plant propagation. This study aimed at investigating the callogenesis and embryogenic potential of new superior cocoa clones on culture media added with various concentrations of antioxidants and variation in the concentration of auxin hormone combined with ascorbic acid. The superior clones used in this study were MCC 01, MCC 02, Sulawesi 3, ICCRI 07, and ICCRI 09. The parameters observed in this study were percentage of embryogenic callus formation, number of secondary somatic embryos, and percentage of embryo somatic cells that develop into planlets. The addition of 2,4,5-T combined with ascorbic acid affected the initiation of cocoa secondary somatic embryogenesis. Treatment combination of ascorbic acid 100 mg.L-1 and 2,4,5-T 1 mg.L-1 had a significant effect on the initiation of secondary somatic embryogenesis of cocoa in the embryogenic callus phase (4.73%), globular embryos (2.53%), torpedo embryos (4.67%) and sprouts (0.47%). Three clones i.e ICCRI 07, Sulawesi 3 and ICCRI 09 clones showed a higher percentage (3.0%, 2.10%, and 1.80%, respectively) of embryogenic callus growth than the other clones. Meanwhile, on MCC 01 clone, all treatments did not affect the regeneration of embryogenic callus. The combination treatment of organic acid and adenine showed a low embryogenic callus response in MCC 01 and MCC 02 clones. However, MCC 02 clone also did not show a response in form of globular, torpedo, and germination phase somatic embryo regenerations. This indicates that different plant cultivars show different responses to the addition of organic acids. Treatment combinations of adenine 0.0125 mg.L-1 + ascorbic acid 100 mg.L-1 gave the best response to the regeneration of somatic embryos for the globular, torpedo, and g 
Consumer Acceptance on Chocolate Drink Made with a Mixture of Commercial Cocoa Powder and Unfermented Cocoa Powder
Full text linkCocoa is one of the foods that contains polyphenol-rich compounds that act as antioxidants. The processing of cocoa into chocolate products generallycauses a decrease in the number of polyphenols. In the development of chocolate beverage products, it is necessary to evaluate sensory evaluation. The purposeof this study was to determine consumer acceptance, total phenolic content (TPC) and antioxidant activity of chocolate drinks made from commercial cocoa powderadded with unfermented cocoa powder. Seventy-five panellist (17-23 years old) contributed to the consumer acceptance of four samples of chocolate drinks witha ratio of commercial cocoa powder: unfermented cocoa powder = 100%:0%, 90%:10%, 80%:20%, 70%:30%. The sample than was analyzed for its antioxidantactivity using the ascorbic acid equivalent antioxidant capacity and total phenolic content using the Folin-Ciocalteau method. The results showed that the sample witha composition of 100% commercial fermented cocoa had the highest value on each sensory attribute. It had the highest TPC (321.235 mg gallic acid equivalent),antioxidant activity (982 ppm), and inhibition percentage (46%), respectively. Addition of the unfermented cocoa powder composition showed a decrease in the level ofconsumer preference. However, the addition did not significantly affect the total phenolic content, but slightly increased the antioxidant activity. The use of unfermentedcocoa powder for mixing components that are not processed standardly has not been well received by consumers, proper processing of unfermented cocoa powderis required if the polyphenol compound is desired. 
Determinants of Compliance with Standard Practices of Pesticide Use Among Cocoa Farmers in Southwestern Nigeria
Full text linkCocoa production in Nigeria is limited due to pests and diseases. In an attempt to control these, farmers apply pesticides which leave chemical residue in cocoa beans. There are established standard practices to address the abuse in use of pesticides. However, there is dearth of information on factors that predispose cocoa farmers to comply with approved standard practices. Therefore, determinants of compliance with standard practices of pesticide use by cocoa farmers in Southwestern Nigeria were investigated. A three-stage sampling procedure was used to select respondents. Two cocoa producing States (Oyo and Ondo) were purposively selected. Two Local Government Areas (LGAs) known for cocoa production were selected from each state. Systematic random sampling was used to select 10% of registered farmers to give 354 respondents. Interview schedule was used to collect data on respondents’ variables. Data were analysed using descriptive statistics and multiple regression at ï¡ 0.05. Most farmers were male (70.3%) and had secondary education (31.9%). Respondents’ age and farmingexperience were 58.0 and 27.0 years, respectively. Farmers’ yield and annual income were 0.449 tonnes obtained from an average of 6.25 ha cocoa farm size andN1,331,519.60 equivalent to $3,698.67, respectively. Determinants of compliance with standard practices of pesticides use for both dosage and procedure were determined by age (ï¢ = -0.20), income (ï¢ = 0.15), exposure to information (ï¢ï€ = 0.36), constraints (ï¢ = -0.27) and accessibility to approved pesticides (ï¢ = -0.11). Exposure of farmers to information and realising more money motivates them to purchase approved pesticides that will aid compliance with standard practices. As age of farmer increases, the ability to comply will be limited. A farmer who is constraint with poor access to recommended pesticides will go for unapproved types which are against compliance. Concerted efforts should be put in place by relevant bodies to ensure sustainable income, information access for respondents in order to adhere to recommended protocols and enhanced cocoa yield
Factors Determining the Characteristics of Cocoa Soil in Sulawesi
Full text linkSulawesi exhibits unique land characteristics as a result of complex geological and geomorphological processes. The information related to what extent of theseprocesses affect regional soil characteristics is still limited. An investigation was performed on cocoa farms from three major cocoa producers, i.e. South Sulawesi, Central Sulawesi, and Southeast Sulawesi. A research was done to point out the key variables contributing to cocoa soil characteristics. Soil survey was performed based on available local information to collect top soil composite samples, i.e. 0–30 cm depth, in the circle of cocoa stand within 50–75 cm radius. A routine soil analysis was done to evaluate soil texture, organic carbon (C), total nitrogen, K, Ca, Mg, soil pH (H2O), and some micronutrients, i.e. Fe, Cu, and Zn. To simplify the complexity, the multivariate data was submitted into principal component analysis (PCA). Additional mapping of soil variable gradient through inverse distance weighting (IDW) interpolation was included in the analysis to improve our interpretations. Data show that the variability for almost all investigated variables is high with coefficient of variation (CV) of up to about 118% for Fe. While low variability with CV about 10% was observed for soil pH (H2O). The PCA demonstrates that the first five principal components (PCs) account for 56.89% of variance in the original variables. Two factors to be considered to explain the variation in the PCs was soil texture and parent material. Further analyses exhibit an association of sand, silt, and clay fractions to Southeast, Central, and South Sulawesi. Mineral compositions, such as K, Ca, Mg, Fe, and Zn, exhibit an important contribution to the parent material factor as they define the variability in the PCs. Additionally, the analysis of gradient maps may indicate the two physical environmental conditions, i.e. landform and geological setting, contribute to the specific characteristics of cocoa soil
Grouping Tolerant Level of Cocoa Genotypes Against Pod Rot Disease in Malaysia
Full text linkCocoa pod rot disease (CPRD), caused by Phytophthora palmivora, is the main disease that caused major losses in Malaysia. It is important for screening the cocoa genotypes available in Malaysia for their tolerance level against the CPRD. This paper has an objective to select the potential genotypes tolerant to P. palmivora by grouping the cocoa genotypes available in Malaysia based on four tolerant levels such as tolerant, moderately tolerant, moderately susceptible and susceptible. The experiment was carried out at the laboratory of Plant Pathology at the Cocoa Development and Research Centre Jengka, Pahang using the detachedpod test. Isolate of P. palmivora was obtained from a naturally infected cocoa pod in cocoa field at the Cocoa Research and Development Centre Tawau, Sabah,Malaysia then inoculated by a single point on the ridges of pod to 40 mature unripe pods of each tested genotypes. Fifty genotypes were tested in this study.The assessed disease severity was the rate of lesion area development from 1 to 7 days after inoculation and the proportion of pod area infected by CPRD. Thedisease severity was significantly different among tested genotypes showing tolerance variability against CPRD. Four nonlinear models consisted of Monomolecularmodel, Exponential model, Logistic model and Gompertz model were used to fit the proportion pod infection area curve. The best fitted Gompertz model was used in calculated the area under disease progress curve (AUDPC). The variability of both disease severity variables was used to group the genotypes into four tolerant levels using the k-means clustering method with 10 genotypes in group I (torelant), 14 genotypes in group II (moderately tolerant), 13 genotypes in group III (moderately susceptible) and 13 genotypes in group IV(susceptible). Six genotypes in group I, namely MCBC 13, PBC 221, BAL 209, KKM 19, QH 1176 and KKM 22 were identified to have lower disease severity values compared to control tolerant genotype PBC 123 that could be suggested to the farmers to be planted in the field. 
Effect of Addition of a Specific Mixture of Yeast, Lactic and Acetic Bacteria in the Fermentation Process to Improve the Quality and Flavor of Cocoa Beans in Colombia
Full text linkCocoa fermentation process is fundamental to generate flavors and aromas that are characteristics of chocolate. In Colombia, this process is carried out by microbiota that spontaneously colonizes cocoa beans, therefore the quality of the fermentation is inconsistent. Taking into account that the fermentation of cocoa beans is carried out by a consortium of microorganisms, the aim of thisresearch was to describe the effect of the addition of a specific mixture of yeasts, acetic acid bacteria, and lactic acid bacteria on the physicochemical and organolepticcharacteristics of cocoa beans (clone CCN 51). Isolates of two yeasts (Wickerhamomyces anomalus and Debaryomyces hansenii), three acetic acid bacteria (AAB), (Gluconobacter japonicus, Acetobacter tropicalis, and Acetobacter pasteurianus) and three lactic acid bacteria (LAB) (Pediococcus acidilactici, Lactobacillus brevis, and Lactobacillus plantarum) obtained fromprevious cocoa fermentations selected for their pectinases and acid production capacities in a specific mixture were used. Using the micro-fermentation technique, the effect of a biological starter was evaluated under different viable microorganismsratios (Yeasts: LAB: AAB as follows, 1: 1: 1, 1: 2: 2, 1: 2: 1, 1: 1: 2, 2: 1: 1, 2: 2: 1, 2: 1: 2, and 2: 2: 2). The concentration of each microorganism was standardized at 1x107 cfu/mL, then the biomass of 4 mL for ratio 1 and 8 mL for ratio 2 of each suspension of microorganisms was added at time zero. Different doses of inoculum were 0%, 1%, 2%, 3%, 4%, and 5% v/w mL inoculum/g cocoa beans. A beneficial effecton the sensory quality of cocoa beans was evidenced by the addition of microorganisms; the best proportion of microorganisms was 2:1:2 (yeasts:LAB:AAB) and the best inoculum dose was 3% (v/w) showing lower acidity, astringency, and bitterness, and emphasizing the cocoa flavors, fruity, nutty, and panela malt. 
Effect of ascorbic acid on chemical content and viability of cocoa seed after storage
Full text linkStorage of oily seeds will make the seeds deteriorated, this caused by auto-oxidation. It will changes the biochemical and physiology of the seeds. Application of Antioxidant can prevent the activity of auto-oxidation. This research aimed to study the effect of ascorbic acid to minimize the deterioration by observing the chemical content and viability of Theobroma cacao L. seeds. It was conducted at kaliwining experimental station, Indonesia Coffee and Cocoa Research Institute, Jember, East Java and at Universitas Kristen Satya Wacana, Salatiga, Central Java. The research used Completely Randomized Design and T-test analysis. The treatments consisted of four ascorbic acid concentrations, i.e. 0 ppm as control, 250 ppm, 300 ppm, and 350 ppm. The treated cacao seeds were stored on two shelf life i.e. 1 week and 2 weeks. Each treatment was repeated with seven replications. Data analysis used SAS 9.0. The results showed that ascorbic acid application can effect on reducing sugar, protein, lipid, free fatty acid content, and viability of cocoa seeds. Ascorbic acid with concentration of 250 ppm and 300 ppm can maintain cocoa seeds viability until 2 weeks of shelf life
Evaluation of Cocoa Agribusiness in South Manokwari Regency, West Papua, Indonesia
Full text linkSouth Manokwari has triumphed with cocoa since 1980 but that glory has faded in recent years. Recently, this regency is faced with various problems for the development of cocoa agribusiness. The purpose of the study was to evaluate the current cocoa-based business in South Manokwari. This research was conductedin three districts in South Manokwari namely Oransbari, Ransiki, and Momi Waren. The study was conducted using the purposive random sampling technique on 119 respondents. The distribution of respondents in the three districts was: Oransbari 44 respondents; Ransiki 42 respondents; and Momi Waren 33 respondents. Basedon the study results, it was known that the average plantation ownership by the farmers was 1.09 ha. Ninety nine percent of farmers were still in productive age (15-65 years). The level of education was still low with 67.13% of farmers had no formal education or did not finish elementary school. Cultivation practices thatmust be improved included pruning (74.71%), fertilization (95.89%), pest and disease control (55.16%), and waste utilization (82.07%). The cocoa beans’ average production was in the range of 71.67–173.33 kg dry beans or 21.67–1000 kg fresh beans per ha harvested from 1,574 ha of farmers’ cocoa land in South Manokwari. Meanwhile, the average prices for cocoa beans were USD 0.24–USD 0.36/kg for the fresh beans and USD 1.20–USD 2.23/kg for the dried beans