Jurnal Ilmu Peternakan dan Veteriner Tropis (JIPVET - Journal of Tropical Animal and Veterinary Science)
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Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya: Isolation and Identification Escherichia coli form Broiler Farm Water Source and Aloe Vera Antibacterial Activity Test
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Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%.
Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity
Abstrak
Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%. Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%.
Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakter
Analisis Jumlah Coliform dan Faecal Coli (MPN) pada Daging Sapi dan Ayam di Kota Manokwari: Analysis of (MPN) of Coliform Bacteria and Faecal Coli on Fresh Beef and Chicken in Manokwari
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Beef and chicken meat are potential as a medium for microbial growth such as coliform bacteria since it has a high nutrients content. Coliform bacteria in a given amount could be used as a hygienic indicator and a sign for the presence of pathogenic bacteria. The aimed of this research was to determine the amount of coliform and Escherichia coli on fresh beef and broiler chicken. The bacterial tests included qualitative tests as presumptive test, confirmed test, completed test whereas quantitavely test as Most Probable Number (MPN) to test the number of coliform and faecal coli. The results showed that pH in beef meat is normal and chicken meat shows under normal pH (pH in beef anf chicken meat are 5,7 – 5,98). Coliform and faecal coli bacteria was detected in 100% of samples had MPN values > 1 MPN/g. It means that the value do not meet eligibility criteria consumption. The high number of Coliform and faecal coli contamination in beef and chicken meat were supposed in relation with market sanitation, cross contamination with other food, low knowledge about hygiene.
Keywords: Number of coliform; Beef and chicken; Traditional market
Abstrak
Daging sapi dan ayam memiliki kandungan nutrisi yang tinggi sehingga menjadi media yang baik untuk pertumbuhan mikroba. Salah satu jenis bakteri yang sering mengkontaminasi yaitu coliform dan faecal coli yang merupakan indikator higienis dan sebagai tanda keberadaan bakteri patogen. Penelitian ini bertujuan untuk menghitung jumlah coliform dan faecal coli pada sampel daging sapi dan ayam yang dijual di pasar traditional kota Manokwari menggunakan uji kualitatif yaitu uji presumptive test, confirmed test, completed test sedangkan kuantitatif yaitu Most Probable Number (MPN) untuk menguji jumlah coliform dan faecal coli. Hasil penelitian menunjukkan bahwa nilai pH daging sapi dalam kisaran normal dan pH ayam tergolong rendah yang semuanya pada kisaran 5,7 – 5,98; dan 100% sampel daging sapi, ayam dan pedagang daging terkontaminasi bakteri coliform dan faecal coli dengan nilai MPN yang tidak memenuhi kriteria kelayakan konsumsi, yakni > 1 MPN/g. Tingginya cemaran coliform dan faecal coli pada daging sapi dan ayam terkait dengan faktor kualitas sanitasi pasar, kontaminasi silang dengan bahan makanan lain dan pengetahuan higienitas yang rendah.
Kata kunci : Jumlah coliform dan faecal coli; Daging sapi dan ayam; Pasar Tradisiona
Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung: The Effect of Sarang Semut (Myrmecodia Sp) Extract in Ringer Laktat Dilution on the Abnormality and Viability of the Spermatozoa of Ayam Kampung (Gallus gallus)
Get PDFAntioxidants can inhibit the oxidation process by reacting with free radicals, which in turn, maintain the quality of spermatozoa. This study aims to determine the viability and the abnormality of “ayam kampung†(Gallus gallus) spermatozoa after being diluted in ringer lactate infused with “sarang semut†(Myrmecodia Sp) extract. RAL is the research method used, utilizing 4 different Gallus gallus as test subjects. The variables observed are the abnormality and the viability of the spermatozoa sample treated with different amounts of Myrmecodia Sp extract, P0: 0 ml, P1: 0.02 ml, P2: 0.03, and P3: 0.04 ml. The abnormality rate for each sample is as follows; P0: 14 ± 4.05%, P1: 13.5 ± 3.78%, P2: 3.5 ± 1.23% and P3: 12.83 ± 5.04 %. Using the variance analysis, the result showed that the addition of Myrmecodia Sp extract in ringer lactate solution had little to no effect on causing spermatozoa abnormalities. Furthermore, the viability of spermatozoa for each sample is; P0: 14 ± 4.05%, P1: 13 ± 3.78%, P2: 13 ± 1.22% and P3: 12 ± 5.04%. Based on the variance analysis, the result showed that Myrmecodia Sp extract had a significant effect (P <0.05) on the spermatozoa viability. The addition of Myrmecodia sp extract to ringer lactate diluter had little to no effect on the abnormality of the Gallus gallus’ spermatozoa but had a significant effect on the spermatozoa’s viability
Identifikasi Cacing Gastrointestinal pada Babi di Kabupaten Jayawijaya dan Paniai, Provinsi Papua: Identification of Gastrointestinal Worms in Pigs in the District of Jaya Wijaya and Paniai, Province of Papua
Get PDFThe rearing of pigs in the mountains of Papua is generally based on the free-range method during the daylight. Poor environmental sanitation and free-range management have been reported as risk factors of gastrointestinal worm infestation in pigs. In general, gastrointestinal parasite infestation can cause a loss of appetite, impede growth, decrease feed conversion rate, and increase vulnerabilities toward other pathogens. To add to that, the acute levels of infestation can cause death. Furthermore, some species can infest humans (zoonotic). As zoonotic parasites can impact the productivity and health of the people, it is crucial to identify the species of such parasites. This research was conducted in Umpakalo village at Kurulu district, Jayawijaya region, and three other villages namely Obaipugaida, Eyagitaida, and Ugituwataida at Ekadide district, Paniai region. Forty-five fecal samples from different pigs were used as the research material. The native method was used to identify the physical appearance of the eggs, and in turn, identify the species of the parasite. The data was analyzed in the tabulation form. The conclusion obtained in this research is that 95% of the fecal samples were infected by the gastrointestinal worms. Identified worms are Ascaris suum, Strongyloides ransomii, Trichuris suis, and Strongyle types. Most pigs have mixed infections
Kualitas Fisik Telur Ayam Petelur pada Tingkat Pelaku Usaha di Kabupaten Manokwari Provinsi Papua Barat: Physical Quality of Layer Eggs Supply at the Level of Local Business in Manokwari Regency West Papua Province
Get PDFPenelitian ini bertujuan untuk mengetahui kualitas fisik, lama penyimpanan dan rantai pemasaran telur ayam ras pasok pada distributor dan pengecer di Kabupaten Manokwari Provinsi Papua Barat. Sampel telur diambil dari tiga distributor dengan lama penyimpanan 12 hari dari pengecer yaitu pasar tradisional dan pasar moderen untuk masing-masing lama penyimpanan 17 hari dan 22 hari. Kualitas fisik yang diukur adalah kedalaman kantong udara, indeks albumin dan indeks yolk serta lama penyimpanan telur yang meliputi 12 hari, 17 hari dan 22 hari. Hasil penelitian menunjukkan bahwa telur ayam ras pasok yang beredar di Kabupaten Manokwari telah mengalami penurunan kualitas fisik. Setelah lama penyimpanan 17 hari, berdasarkan badan standar nasional telur ayam ras pasok tidak layak dikonsumsi sehingga seharusnya tidak dipasarkan
EFEK PEMBERIAN EKSTRAK KAYU AKWAY (Drymis Sp) TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus L): The Effect of Akway Wood Extract (Drymis Sp) on The Mice (Mus musculus L) Spermatozoa Quality
Get PDFThis research was aimed to study the effect of Akway wood (Drymis Sp) extract treatment on spermatozoa quality of mice (Mus musculus L). The extract was given orally once a day in 2 weeks. The animals were divided into three groups; one control group (K1) and two treatment groups with four replicates. K2 group was given 1.3 ml/gr body weight / day and K3 group was given 3.3 ml/ gr body weight / day. The results indicated that treatments were increased significantly (P<0.05) on acrosome integrity and viability, while on the motility, concentration and abnormality treatments had no significant effect.
 
POTENTIAL BAGGASE AND MOLASSES WASTE SUGAR FACTORY FOR MIXED SUBSTRATES WITH COW MANURE IN FERMENTATION PROCESS METHANOGENIC
Get PDFThis study aims to analyze the potential of the sugar mill waste, namely baggase and molasses as a mixture of cow manure as a substrate in a methanogenic fermentation. This study uses 4 treatment that is P0 as controls (100% cow manure), later in other treatment using a mixture of cow manure comparison: baggase: molasses, P1 (50:20:30), P2 (50:30:20) and P3 ( 50: 40; 10). Parameters measured were nitrogen, carbon, ratio C / N and pH value. The analyze used in this research is statistic analysis. The results were obtained P3 which is a mixture of substrate (50:40:10) has an average value of the content of nitrogen, carbon, ratio C / N, and high pH value is compared to the other treatments. Ratio C / N at P3 of 24.47 closest Ratio C / N ideal for methanogenic fermentation which amounted to 25. The pH value of the treatment amounted to 7.05 billion P3 also meet the standards for process fermentation ranging from 6.6 to 7.6. 
Kualitas Fisik Antimicrobial Edible Film (AmEF) dengan Ekstrak Daun Teh (Camellia sinensis) dari Gelatin Limbah Tulang Ayam: Antimicrobial Edible Film (AmEF) with Tea Leaf Extract (Camellia sinensis) made From Chicken Waste Gelatin Physical Quality
Get PDFThis study intends to find out the impact of green tea leaves’ concentration and heating temperature on the physical quality of the antimicrobial edible film (AmEF) made from chicken bone gelatin. This study also utilizes chicken bone waste, in oder to increase both monetary value and the usefulness of that waste. The bone gelatin will then be utilized as AMeF and combined with green tea leaves’ extract (Camellia sinensis) that used as sausage wrapper. Several stages have been taken placed as follow: chicken bone gelatin production; green tea leaves extraction; and tea leaves’ extract-AMeF production. cCompletely randomized design with the factorial pattern was used in this study. Data was statistically analyzed using uni-variate analysis with 5% significance rate. Duncan’s Multiple Range Test (DMRT) will later be used to further test if there is a difference between each treatment. The physical qualities of AmEF that tested in this study include clarity, color, and thickness. The result of this study shows that a clarity value of AmEF was ranged between 0.62-2.12 abs, color was 0.87-2.60 abs, and 0.015-0.023 mm of thickness. The addition of tea extract and heating temperature have significant (P<0.05) effect on the clarity and color while have no effect on the thickness
ANALISIS FUNGSI BIAYA STOCHASTIC FRONTIER USAHA PETERNAKAN AYAM PEDAGING PADA PETERNAK YANG MENGGUNAKAN OPEN HOUSE SYSTEM: STOCHASTIC FRONTIER COST FUNCTION ANALYSIS OF BROILER BUSINESS FOR OPEN-HOUSE SYSTEM FARMERS
Get PDFThis study aims to analyze factors that influence the cost function of broiler farms using open house system. The results indicates that all factors included in the model, simultaneously, affect the production costs. Partially, input such as dayold chick (DOC) costs, feed costs, electricity costs, production positively affect the system. Labor costs on the other hand, have a negative effect on the system. The cost of drugs and fuel has no effect on broiler production costs using the open house system
PRODUKSI BAHAN KERING DAN TINGGI TANAMAN RUMPUT SUDAN (Sorghum sudanense) PADA DEFOLIASI KE DUA TANPA PUPUK NITROGEN ULANGAN: THE DRY MATTER PRODUCTION AND HEIGH OF PLANT OF SUDAN GRASS (Sorghum sudanense) AT SECOND HARVEST UNDER DIFFERENT PLANT DISTANCES WITHOUT NITROGEN FERTILIZATION
Get PDFThe aim of this study were to obtain the dry matter (DM) production of sudan grass at second harvest under different plant distances without nitrogen fertilization and to decide whether fertilization have to be added or not. The design of the study was split plot design 4 x 7 with three blocks. The main plots were plant distances, namely :20x40 cm (J1), 40x40 cm(J2), 60x40 cm (J3) and 80x40 cm (J4), and each plot was divided into 7 subplots for randomly N application treaments, namely :N0 (P0); N75 (P1); N150 (P2); N225 (P3); N300 (P4); N375 (P5) dan N450 (P6). Phosphorus and K fertilizer were given at the same rate, that were 300 kg P2O5 and 150 kg K2O. Result of the study showed that planting distances, level of fertilizers and the interaction were significant (P<0.05) affected DM production and height of plants in the second harvest. The DM production in the second harvest was significantly lower (P<0.05) than the DM production in the first harvest. From this study it can be concluded that N fertilizer application should be given again after cutting in order to maintain the DM production of sudan grass in the next harvest