Central Food Technological Research Institute
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Nanocomposite-Based Electrochemical Sensor for the Detection of Ethyl Paraoxon, an Acetylcholinesterase Inhibitor, in Food Matrices with Validation via Gas Chromatography
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Apoptotic and anti-inflammatory effect of nisin-loaded sodium alginate-gum arabic nanoparticles against colon cancer cells
No full textColon cancer is one of the leading causes of mortality and morbidity worldwide. Nisin, a polycyclic antibacterial
peptide and food preservative has shown potential to combat cancer. However, it is susceptible to proteolytic
cleavage in the gut. The current study investigates the protective and cytotoxic effects of nisin loaded sodium
alginate gum arabic nanoparticles (Nis/ALG-GA NPs) in Caco2 cells. The physicochemical properties, loading
efficiency and release kinetics were studied. Cytotoxicity (MTT assay), apoptotic effect (Ethidium bromide and
acridine orange staining) and internalisation (FITC tagging) were evaluated. Gene expression of apoptotic
markers and IL-10 were analysed by qPCR. The Nis/ALG-GA NPs were spherical, small with a smooth outer
surface and mean size of 193 ± 4 nm. The loading efficacy was 88 ± 2 % exhibiting slow sustained release of the
peptide under different gut pH conditions. The IC50 value obtained was 500 μg for 48 h and 80 μg for 72 h of
incubation. The Nis/ALG-GA NPs were internalised into Caco2 cells and induced apoptosis with an increased
expression of bax gene and converse decrease of bcl-2 gene. Anti-inflammatory gene IL10 was upregulated upon
treatment with NPs. Thus, the Nis/ALG-GA NPs may be promising oral drug delivery systems against colon
cancers
Natural plant-derived nanovesicles for effective psoriasis therapy via dual modulation of IL-17 and NRF2 pathway
No full textPsoriasis is an inflammatory skin disease with aberrant epidermal differentiation and chronic inflammation.
Plant-derived exosome-like nanovesicles (PDNVs) are exosome-mimetic structures isolated from plants
with enhanced bioavailability and solubility. Herein, we investigated the therapeutic efficacy of PDNVs for
psoriasis treatment. In our initial phenotype-driven screen, PDNVs from apple, garlic, and shallot displayed
both pro-differentiation and anti-inflammatory activity in vitro. In imiquimod (IMQ)-induced psoriasis-like mu
rine model, topical application of either shallot or garlic PDNV hydrogels led to complete recovery of normal
skin phenotype. In porcine skin ex vivo assay, both shallot and garlic PDNVs displayed percutaneous pene
tration up to the suprabasal epidermis. Using next generation sequencing and quantitative mRNA profiling in
keratinocytes and murine skin, we elucidated a suppressive effect of shallot and garlic PDNVs on IL-17
signaling via modulation of upstream nuclear factor erythroid-2-related factor 2 (NRF2) pathway. Since
PDNVs are economical to produce, shallot and garlic PDNVs could be natural and cost-effective topical alter
native for psoriasis