Central Food Technological Research Institute
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Multifunctional tetrahydrocurcumin reinforced methylcellulose films for shelf life evaluation of chicken meat
No full textTetrahydrocurcumin (THC) (0.5 %, 1.0 %, and 3.0 % concentrations) incorporated methylcellulose (MC) com-
posite films were developed using the solution casting technique. THC, a colourless and more stable curcumin
derivative, offers enhanced bioactivity while overcoming limitations of curcumin, such as poor photostability
and strong pigmentation. Scanning electron microscopy revealed smooth morphology for neat MC and MC/THC
(0.5–1.0 %) films, while the MC/3.0 % THC film exhibited a textured surface indicative of THC aggregation at
higher loading. Water contact angle increased from ~45◦ (neat MC) to ~64◦ (MC/3.0 % THC), demonstrating
improved hydrophobicity. Water vapour permeability was reduced by ~34.5 % for the MC/3.0 % THC film,
highlighting better moisture barrier performance. The MC/THC films showed dose-dependent enhancement in
antioxidant (DPPH and ABTS), UV-blocking, and antimicrobial activity against E. coli and S. aureus. The MC/THC
film with 3.0 % THC extended the shelf life of raw chicken meat stored at 4 ± 2 ◦C, up to 5 days. Additionally,
TBARS analysis revealed a slower increase in lipid oxidation in meat samples wrapped with MC/3.0 % THC film
Distinguishing native trisaccharides having differential monosaccharide composition and linkage using PGC-LC MS/MS
No full textNative glycosyl sucrose derivatives in plants represents primarily the trisaccharide isomers having different
monosaccharides composition and linkage specificity. They can occur as complex mixtures wherein the sepa-
ration and identification of individual trisaccharide becomes challenging. Herein, we employed porous graphitic
carbon (PGC) liquid chromatography coupled to mass spectrometry to evaluate the elution order of major
glycosyl sucrose trisaccharides and determined the structural feature through tandem mass spectrometry (MS/
MS). Major trisaccharides comprised of galactosyl-, glucosyl- and fructosyl sucrose derivatives along with few
reducing trisaccharides revealed distinct elution order. Furthermore, the application of this approach to profile
the oligosaccharides in grape seeds showed the separation and identification of multiple native trisaccharides in
the complex mixture that were hitherto unknown
Chemical composition and bioefficacy of Pimenta dioica, Cinnamomum verum, and Eucalyptus globulus essential oils against stored-product beetles
No full textThe study evaluates the chemical composition and insecticidal properties of essential oils (EOs) from Pimenta
dioica, Cinnamomum verum, and Eucalyptus globulus as sustainable alternatives to chemical fumigants for stored
grain protection. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified key compounds: 3-Allyl-6-
methoxyphenol, tris(tert-butyldimethylsilyloxy)arsane, and limonene in P. dioica oil; methyl eugenol and cin-
namyl ester in C. verum oil; and eucalyptol and α-pinene in E. globulus oil. These bioactive components are linked
to their insecticidal efficacy. Fumigant activity was assessed, revealing LC50 values after 24 h of exposure for
S. oryzae as 39.9, 147.8, and 38.2 μL L 1, and for T. castaneum as 24.9, 74.9, and 45.3 μL L 1, respectively.
Among the EOs, E. globulus demonstrated the highest fumigant toxicity, achieving 100 % mortality at 425 μL/L
within 72 h. Contact toxicity tests showed LD50 values of 0.69, 0.31, and 0.88 μL/cm2 for S. oryzae, and 1.09,
0.61, and 1.58 μL/cm2 for T. castaneum after 24 h, indicating moderate effectiveness. The findings underscore the potential of E. globulus as a strong fumigant and C. verum for contact toxicity, attributed to compounds like
eucalyptol and methyl eugenol, respectively. These results highlight the potential of these EOs, particularly
E. globulus, as eco-friendly alternatives for managing S. oryzae and T. castaneum infestations in stored grains,
reducing reliance on synthetic chemical fumigants
Characterization of foxtail millet protein concentrate extracted using conventional, novel methods and its application
No full textTargeted next-generation sequencing for pathogen and antimicrobial resistance (AMR) identification and profiling
No full textVolatile fingerprinting of allspice (Pimenta dioica L.) leaf essential oil by GC-MS, E-Nose and NMR
No full textThe study explores the Allspice (Pimenta dioica L.) leaf essential oil (AEO), employing green techniques to enhance yield
and chemical composition, thereby broadening its applicability in food, pharmaceutical, flavoring, and cosmetic indus-
tries. Enzyme-assisted methods, particularly cellulase and viscozyme, significantly increased the AEO yield (2.8 ± 0.5%
and 3.28 ± 0.25%) while maintaining its physical properties. The chemical composition of AEO was analyzed using gas
chromatography-mass spectrometry (GC-MS), fourier transform infrared spectroscopy (FTIR), and nuclear magnetic reso-
nance (NMR), including 1H, 13C, and 2D NMR techniques like HSQC and HMBC. Antioxidant activity was determined
using the ABTS assay, while aroma profiling was conducted using an electronic nose (E-Nose) combined with principal
component analysis (PCA) and soft independent modeling of class analogy (SIMCA). Enzyme pretreatment boosted
total phenols by 26% as well as antioxidant activity (87.2 ± 0.75 to 90.83 ± 0.75 mg TE/ml) through complex enzyme
interactions, indicating improved bioactivity. Eugenol (74.43%), β-myrcene (10.75%), p-chavicol (5.00%), and limonene
(2.67%), are major compounds identified by GC-MS. ATR-FTIR confirmed aromatic ethers, carboxylic acids, alkanes,
alkenes, and alkynes in 750–2900 nm. NMR spectroscopy differentiated β-pinene and β-myrcene (both C10H16 isomers),
while providing detail insight into eugenol, p-chavicol, β-myrcene, and caryophyllene. Aroma profiling using an E-Nose
revealed distinct volatile compound patterns, visualized using PCA and SIMCA. This study insights into the aroma, flavor,
and chemical composition of Allspice leaves, highlighting its potential for diverse industrial applications and promoting
green extraction process
Study on effect of composite nano absorbent on adsorptive removal of nitrate from sugar industry wastewater
No full text
Evaluating the efficacy of Monarda citriodora essential oil as a biorational against Rhyzopertha dominica infestation in stored sorghum and pearl millet
No full textSorghum and pearl millet are vital sources of food and fodder in semi-arid regions, prone to infestation by stored grain insect pests. Essential oil (EO) from the Lamiaceae family plants is gaining attention because of its effectiveness against these pests. We report for the first time on the utilization of essential oil from Monarda citriodora (MC-EO) against Rhyzopertha dominica (RD), a significant pest prevalent in both stored sorghum and pearl millet. The current work highlights fumigant and contact bioassays (without and with grains), germination ability of post-treated grains, EO residual analysis, and in vitro enzyme assays (AChE and GST). The M. citriodora exhibits fumigant (FT) and contact (CT) toxicity in 12 h with an LC50 of 48.94 μL/L and 0.005 μL/cm2, respectively. Moreover, with sorghum and pearl millet for 168 h, FT was 494 μL/L and 272.68 μL/L. The EO treatment showed no residues on grains without any significant change in germination. Inhibition (in vitro) of AChE and GST activity was noticed with 10 % EO. Computational studies with the major components of EO (thymol and o-cymene) showed the binding affinities towards RD AChE and observed to be −6.1 kcal/mol and −5.5 kcal/mol, respectively. Hence, M. citriodora EO can be a good biorational candidate against R. dominica
Insights on mitigation, fumigant persistence and oviposition deterrence of Callosobruchus chinensis using Ocimum gratissimum essential oil.
No full textAn increased human population rate has advanced the need to manage production and shelf life of food grains.
Significant loss of food grains by insect pests has led to the overuse of chemical pesticides for their management.
The present study focuses for an alternative to synthetic pesticides, a natural derived insecticidal biorational from
Ocimum gratissimum (O. gratissimum) essential oil (EO) against Callosobruchus chinensis (C. chinensis) which is a
notable pest responsible for quantitative and qualitative loss of stored pulses. The EO obtained from unique
Ocimum chemotype contains thymol (26.67%), gamma terpinene (19.98%) and o-cymene (13.54%). The
insecticidal toxicities (fumigant and contact) of EO showed significant death rate (100%) of C. chinensis at 24 h.
Additionally, EO concentrations with 10%–50% showed good oviposition deterrence of C. chinensis. SEM analysis
of EO treated C. chinensis exhibited disintegrated setae on elytral surface. Besides, treated grains has revealed no
significant residual persistence of aroma and retained germination. Hence, O. gratissimum EO might be a potential
biorational entrant for the effective control of C. chinensis