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Systematic simulation of the interactions of Pleckstrin homology domains with membranes: dataset
No full textPleckstrin homology (PH) domains can recruit proteins to membranes by recognition of phosphatidylinositol phosphate (PIP) lipids. Several family members are linked to diseases including cancer. In Le Huray et al., "Systematic simulation of the interactions of Pleckstrin homology domains with membranes", Science Advances, 2022, we report the systematic simulation of the interactions of 100 mammalian PH domains with PIP-containing membranes. This dataset contains raw coarse-grained molecular dynamics (CG MD) simulation data for all 100 individual PH domains
Data associated with 'Single residue switches of aggregation in the N-terminal P1-region of alpha-synuclein'
Get PDFAlpha-synuclein (αSyn) is an intrinsically disordered protein (IDP) involved in neurodegenerative disorders including Parkinson’s disease. Previous studies have shown that αSyn aggregation is controlled by interactions between the N- and C-terminal regions with the central aggregation-prone NAC-region. The closely homologous (>50% sequence identity) γ-synuclein (gSyn), by contrast, is resilient to aggregation, despite retaining the NAC-region. Here, using an alanine-scan of a 7-residue sequence in the N-terminus of αSyn previously identified as a ‘master-controller’ of aggregation (P1: residues 36-42), we identify single residues (L38, Y39 and S42) that individually control the aggregation of this IDP in vitro and in C. elegans models. Substituting residues in the P1 region of aSyn individually to those that differ in gSyn (L38M and S42A) inhibits aggregation of aSyn, while switching residues in γSyn to those in αSyn (M38L, A42S) does not change the low aggregation propensity of the γSyn variants. Using NMR-PREs and peptide binding experiments, we show how P1 synergises with residues in the NAC and C-terminal regions to initiate aggregation. Together the results demonstrate a remarkable specificity of the inter-residue interactions that control aggregation of an IDP despite their weak and transient nature
Cholera toxin B-subunit glycopeptide conjugates
Get PDFNMR spectroscopy data for galactosamine derivatives; polyacrylamide gel electrophoresis data for sortase-mediated protein ligation reactions; protein mass spectrometry data for oxime-mediated protein ligation reaction
Data from the 2017 measurement campaign in Barbados (Ice-B). [Dataset].
No full textA dataset from a 6 week campaign at Ragged Point in Barbados focused on measuring atmospheric ice nucleating particles
Dataset associated with 'Directed delivery of terahertz frequency radiation within a dry 3He dilution refrigerator'
No full textContains electrical transport data, temperature log files, QCL characterization, and a few other Misc
Dataset for the study of Enhancing the spectral range of plant and bacterial Light-Harvesting pigment-protein complexes with various synthetic chromophores incorporated into lipid vesicles
No full textThe Light-Harvesting (LH) pigment-protein complexes found in photosynthetic organisms have the role of absorbing solar energy with high efficiency and transferring it to reaction centre complexes. LH complexes contain a suite of pigments that each absorb light at specific wavelengths, however, the natural combinations of pigments within any one protein complex do not cover the full range of solar radiation. Here, we provide an in-depth comparison of the relative effectiveness of five different organic “dye” molecules (Texas Red, ATTO, Cy7, Dil, DiR) for enhancing the absorption range of two different LH membrane protein complexes (the major LHCII from plants and LH2 from purple phototrophic bacteria). Proteoliposomes were self-assembled from defined mixtures of lipids, proteins and dye molecules and their optical properties were quantified by absorption and fluorescence spectroscopy. Both lipid-linked dyes and alternative lipophilic dyes were found to be effective excitation energy donors to LH protein complexes, without the need for direct chemical or generic modification of the proteins. The Förster theory parameters (e.g., spectral overlap) were compared between each donor-acceptor combination and found to be good predictors of an effective dye-protein combination. At the highest dye-to-protein ratios tested (over 20:1), the effective absorption strength integrated over the full spectral range was increased to ~180% of its natural level for both LH complexes. Lipophilic dyes could be inserted into pre-formed membranes although their effectiveness was found to depend upon favourable physicochemical interactions. Finally, we demonstrated that these dyes can also be effective at increasing the spectral range of surface-supported models of photosynthetic membranes, using fluorescence microscopy. The results of this work provide insight into the utility of self-assembled lipid membranes and the great flexibility of LH complexes for interacting with different dyes
Translations and transformations in patienthood: cancer in the post-genomics era
Get PDFA collection of interviews with patients, former patients, carers, advocates and professionals about their perspectives and experiences of targeted treatments for cancer
Human hip joint impingement shape and motion model: input and output data for an initial study of eight typical cam-type hip shapes
Get PDFThis dataset contains the input and output data for a computational study of femoroacetabular impingement. The model is composed of a simplified shape model of the human hip joint and the use of hip movement data from multiple activities and multiple subjects. The relevant study is a first demonstration of the capability of the model, which illustrates the effects of the location of a specific femoral bony shape feature on hip joint impingement measures. The details of the shape model design and the values selected for this study are included. The raw hip joint motion data is available in a separate dataset which is linked to this one. The raw outputs from the modelling performed for this study are also included
Stay on the beat with tensor-valued encoding [Dataset and Code]
Get PDFData and Matlab code used for the publication Lasič et al. 'Stay on the beat with tensor-valued encoding: time-dependent diffusion and cell size estimation in ex vivo heart'