Bioscientia Medicina - Journal of Biomedicine and Translational Research
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Effect of Okra Fruit (Abelmoschus esculentus) Extract on Granzyme Expression and Tumor Mass Diameter of Adenocarcinoma Mammae: An In Vivo Study
Get PDFBackground: Breast cancer is currently one of the most common types of cancer suffered by women, with the highest prevalence in all countries in the world. Herbal plants are thought to increase the immune system against tumors, one of which is okra (Abelmoschus esculentus) has the potential as an anti-tumor and anti-carcinogenic. This study aimed to assess the efficacy of ethanol okra extract on granzyme expression and tumor mass diameter in mammary adenocarcinoma of rats treated with adriamycin-cyclophosphamide chemotherapy.
Methods: This study is an experimental study using 28 Sprague Dawley Rats. Data analysis was performed with SPSS version 21 to assess differences in granzyme expression and tumor size.
Results: The combination of chemotherapy and okra fruit extract (P3) showed optimal potential in increasing granzyme expression and reducing mammary tumor size.
Conclusion: The okra fruit extract has potential in the treatment of mammary carcinoma by increasing the expression of granzymes so as to reduce the size of breast carcinoma tumors in vivo
Correlation of LMP-1 Expression with BCL11B in Undifferentiated Type Nasopharyngeal Carcinoma
Get PDFBackground: Nasopharyngeal carcinoma (NPC) is an endemic malignancy in Asia and in Indonesia with a high incidence and mortality. The high mortality rate of patients with NPC is caused by recurrence and metastases early in the disease process, even though they have been given a combination of standard NPC therapy, namely radiotherapy and chemotherapy. Recurrences generally occur after the initial modalities of radiotherapy. Toxicity due to therapy given over a long time can also increase mortality. This study aims to determine the expression of BCL11B in undifferentiated NPC and its correlation with LMP-1 so that it can provide an overview of the nature of CSC in NPC, which is thought to cause recurrence and metastases and provide a poor prognosis in patients with NPC.
Methods: This study was an analytical observational study using a cross-sectional approach using 30 samples from nasopharyngeal biopsy tissue diagnosed with undifferentiated NPC at the anatomical pathology installation of Dr. Saiful Anwar General Hospital, Malang, Indonesia, between 2018-2020. LMP-1 and BCL11B expression was examined using the immunohistochemical method. Data analysis was performed univariate and bivariate with the help of SPSS software.
Results: Statistical analysis using the Spearman correlation test between LMP-1 and BCL11B expression in undifferentiated NPC biopsy tissue showed a significant correlation (p=0.004) with a correlation coefficient of r=-0.511.
Conclusion: The higher the expression of LMP-1, the lower the expression of BCL11B in nasopharyngeal biopsies of patients with undifferentiated
Iron-Deficiency Anemia Related to Ancylostoma duodenale Infection in 67 Years Old Female Patient
Get PDFBackground. Ancylostomiasis is an infection caused by hookworms. It is estimated that hookworms infect 1.2 billion people worldwide and cause higher morbidity than other types, mainly due to iron deficiency anemia. Hookworm infection increases with age. The main clinical manifestations are abdominal pain, features of iron deficiency anemia and hypoproteinemia. Diagnosis is based on the finding of characteristic eggs in the stool.
Case presentation. A 67-year-old female patient had been administered into the Internal Medicine Ward of Dr. M. Djamil Padang Hospital with the main complaint of weakness, fatigue, and lethargy which had increased since 3 days before being admitted to the hospital. The patient also had complaints of pale skin, fever, heartburn, decreased appetite and weight loss. The patient was admitted to the hospital with awareness of compost mentis, cooperative, and his general condition appeared to be moderately ill, blood pressure 130/90 mmHg, pulse rate 104 x/minute, regular pulse, adequate filling, respiratory rate 22 x/minute, temperature 37.8 C. On physical examination, the conjunctiva of the pantient’s eyes were anemic. Laboratory examination revealed Hemoglobin 4 g/dl, Hematocrit 16%, MCV/MCH/MCHC 62fl/15pg/25g/dL, Reticulocytes 0.5%, SI/TIBC 22/315 mg/dL, Ferritin 8.5 ng/mL, and peripheral blood smear showing severe hypochromic microcytic anemia and relative eosinofilia. Ancylostoma duodenale worm eggs were found on routine stool examination. Final diagnosis was Severe microcytic hypochromic anemia caused by an iron deficiency and Ancylostomiasis. Severe microcytic hypochromic anemia caused by iron deficiency and ancylostomiasis was established based on the patient’s history, physical examination and laboratory investigations.
Conclusions. Hookworms infect many people worldwide and cause high morbidity, mainly due to iron deficiency anemia. The highest prevalence is found in the tropics and subtropics, including Indonesia. The main clinical manifestations are abdominal pain, features of iron deficiency anemia and hypoproteinemia. Diagnosis is based on the finding of characteristic eggs in the stool. Albendazole 400 mg single dose resulted in 80% cure and a dose of 200 mg/day for 3 days gave 100% cure. Treatment of anemia is by administering ferrous sulfate or ferrous gluconate orally 200 mg three times a day and continued for up to 3 months after normal hemoglobin levels are achieved to maintain iron stores
Gynaecologic History During Adolescence to Predict Endometriosis Earlier
Get PDFBackground. Endometriosis is a chronic gynecological disorder that generally affects women of reproductive age which is characterized by implantation of endometrial tissue, both glands and stroma outside the uterus. Research shows that the initial onset of endometriosis patients begins in adolescence. The high prevalence with atypical symptoms leads to long term delay in establishing the appropriate diagnosis. Further developments in the field of early endometriosis screening will greatly assist clinicians, especially general practitioners in areas with limited health facilities, in early detection of endometriosis.
Methods. Using the electronic databases, comprehensive literature searches were conducted with the specific keywords. Full-text manuscripts published were reviewed for relevancy and importantly, reference lists were cross-checked for additional relevant studies.
Results. Dysmenorrhea, chronic pelvic pain, dyspareunia, dysuria, dyschezia, and glycoprotein were found dominantly in all analyzed literature. The occurrence of endometriosis affects the functioning of women in multiple aspects of life. Endometriosis places a significant burden on teens and adult women, their families, and society as a whole.
Conclusion. Combination of structured past history taking, and several biomarker level might be useful as a screening tool that easy to use and cost effective, as early diagnosis is expected to prevent further progression of endometriosis especially in adolescence
Correlation Epidermal Growth Factor Receptor Mutation with Non-small Cell Lung Cancer in Passive Smokers: A Review
Get PDFEpidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular epidermal growth factor binding domain and an intracellular tyrosine kinase domain through signaling pathways to regulate cellular proliferation. Epidermal growth factor receptor binding to its ligand will result in autophosphorylation by intrinsic tyrosine kinase activity, thereby triggering multiple signal transduction cascades. Constant or sustained activation of these downstream target sequences is thought to result in a more aggressive tumor phenotype. Mutations in EGFR are associated with non-small cell lung cancer in passive smokers
The Role of Health Officer Related to Adherence to Iron Tablet Supplementation among Pregnant Women in Central Aceh in 2021
Get PDFBackground. Coverage of Fe tablets in Aceh Tengah District is very low, even lower than other districts in Aceh; namely 12.4 percent for Fe 1 and 10.56 percent for Fe3. Failure to distribute these tablets in this district may lead to an increase in the prevalence of anemia. One of the reasons is that the monitoring, recording, and reporting system by health officers in consuming Fe tablets and in routinely checking Hb levels is not good enough. This study aims to analyze the effect of the role of leadership (customers, communicators, motivators, facilitators, and counselors) on the adherence of pregnant women to consuming Fe tablets in the Central Aceh Regency in 2021.
Methods. This type of research is a survey with a cross-sectional approach. The population is all pregnant women in the Aceh Tengah Regency area with a gestational age of more than 20 weeks, or it could be less than 20 weeks, provided that this is the first visit and has made a health visit at the health center. Methods of collecting data by interviewing and checking the health development of pregnant women with Editing, Coding, Entry, and Cleaning. Data were analyzed by univariate, bivariate, and multivariate.
Results. The results of the multivariate analysis with logistic regression obtained the facilitator variable has the greatest influence on compliance in consuming Fe tablets with a value of OR = 14,166 meaning pregnant women who accept the role of health officers as facilitators will obediently consume Fe tablets as much as 14,166 times when compared to mothers who do not accept the role of health officers as customers.
Conclusion. The low adherence to Fe tablet consumption was due to the fact that the number of Fe tablets given by health officers to pregnant women in Aceh Tengah Regency was not up to the standard, which was 90 tablets. Customers and facilitators have a relationship with the adherence of pregnant women to consuming Fe tablets. The facilitator has the greatest influence on compliance in consuming Fe tablets
Latent Syphilis Unresponsive to Doxycycline and Azithromycin
Get PDFBackground. Syphilis management is based on the syphilis stage classification. The main treatment for syphilis is currently benzathine penicillin. However, patients allergic to penicillin antibiotics or who are not willing to be injected can be given doxycycline or erythromycin antibiotics.
Case presentation. We present a case of a female with latent syphilis. Patient was previously treated with oral antibiotics, such as doxycycline for 30 days and followed by azithromycin for 30 consecutive days. Clinical laboratory examinations show increase of TPHA and VDRL, and the case was considered as unresponsive case. Later, the patients received benzathine penicillin G for three weeks and showed clinical improvement.
Conclusion. For patient who refuse to be treated with penicillin injection, the alternative treatments are oral antibiotics. However, there is a reduced number of successful treatments for patients treated with oral antibiotics
Intraventricular Hemorrhage in Children with COVID-19 Confirmed
Get PDFBackground. The most common symptom of Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 is respiratory symptoms. However, neurological symptoms in adult patients are increasingly being reported. In children, neurological symptoms of COVID-19 are still underreported. This case report was aimed to describe intraventricular hemorrhage in a child with Covid-19 infection.
Case presentation. We report a case of a 15-year-old girl with intraventricular bleeding, which is one of the COVID-19 infection symptoms or a possible symptom of a multisystem inflammatory syndrome in children (MIS-C). Laboratory tests on the first day of treatment showed an increase in leukocytes and decreased lymphocytes. On the 6th day of treatment, the patient had worsening symptoms of consciousness and high fever. The results of laboratory examination showed a decrease in kidney function and an increase in D-dimer.
Conclusion. Severe clinical manifestations of COVID-19 can be in the form of neurological manifestations, one of which is intraventricular hemorrhage
Metabolic Control Status Related to Quality of Life in Children with Type 1 Diabetes Mellitus at Dr. M. Djamil General Hospital Padang
Get PDFBackground. Type 1 Diabetes Mellitus (T1DM) is a chronic autoimmune disease requiring lifelong treatment. Good metabolic control could maintain an optimal quality of life. HbA1C is a reliable parameter of metabolic control. Poor metabolic control results in decreased quality of life for T1DM. This study aims to evaluate the relationship between metabolic control status and quality of life in children with T1DM at Dr. M. Djamil General Hospital Padang.
Methods. This cross-sectional study on 52 T1DM children aged 8–16 years using consecutive sampling methods at the pediatric endocrinology outpatient clinic, Dr. M. Djamil General Hospital Padang, from April-September 2021. HbA1C was calculated using the High-Performance Liquid Chromatography (HPLC) method and classified into good and poor control. Quality of life was assessed using PedsQl 4.0 and classified as impaired and unimpaired. The Chi-square test was used for data analysis. The P-value of <0.05 was considered statistically significant.
Results. The majority of respondents were girls (57.7%), and most of them had good nutritional status (73.1%). The mean age at diagnosis was 11.21±2.57 years, and they had suffered from T1DM for 2.21±1.28 years. A family history of diabetes was found in 57.7% of respondents. Complications found were hypoglycemia (19.2%) and diabetic ketoacidosis (50%). Poor metabolic control was found in 78.8% of respondents. Quality of life was impaired in 40.4% of respondents, especially in school functions. This study found that respondents with impaired quality of life had poor metabolic status (p<0.034).
Conclusion. T1DM patients with poor metabolic control status have impaired quality of life
Relationship between Initial CT Value, Inflammatory Markers, and Onset of Clinical Symptoms with Outcomes of Treatment Outcomes of COVID-19 Patients at Dr. M. Djamil General Hospital Padang
Get PDFBackground: Treatment given for COVID-19 includes the provision of antiviral, anti-inflammatory, immunomodulatory, anticoagulant, and treatment of comorbidities and complications. The success of treatment in COVID-19 patients does not only depend on the drug given but is also influenced by clinical manifestations, clinical phase, and the presence or absence of complications and comorbidities. This study aims to assess the relationship between initial CT values, inflammatory markers, and symptom onset on treatment outcomes for COVID-19 patients at Dr. M. Djamil General Hospital Padang.
Methods: This study is an observational analytic study with a retrospective cohort approach in confirmed COVID-19 patients treated at Dr. M. Djamil General Hospital Padang between 1st January – and 31st May 2021. The sample is the population that meets the inclusion criteria; confirmed COVID-19 from the results of RT PCR/TCM SARS-CoV-2, aged > 18 years, including the clinical degree of moderate, severe, critical illness. Exclusion criteria were patients with incomplete medical record data. Univariate analysis is intended to describe each research variable so that the distribution and percentage of each variable are presented in tabular form. Bivariate analysis using Chi-Square statistical test to see the relationship between variables.
Results: COVID-19 patients being treated at Dr. M. Djamil General Hospital Padang were mostly in the age group above 50 years, namely 59.6%, and the female sex was treated more than the male, namely 55.5%. The moderate clinical grade is the highest proportion of hospitalized patients, namely 64.3%, followed by critical clinical 30.0% and severe clinical 5.6%. There is no relationship between the initial CT value and the outcome of COVID-19 patients. There is a relationship between IL-6 levels (initial) with the outcome of Covid-19 patients at Dr. M. Djamil General Hospital Padang. There is no relationship between the onset of clinical symptoms and the outcome of Covid-19 patients at Dr. M. Djamil General Hospital Padang.
Conclusion: There is a relationship between IL-6 levels (initial) with patient outcomes, need for oxygen therapy, ferritin levels with the incidence of sepsis, initial albumin levels with initial D-Dimer levels, and IL-6 levels with D-dimers in COVID-19 patients at Dr. M. Djamil General Hospital Padang