Bioscientia Medicina - Journal of Biomedicine and Translational Research
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Telmisartan Prevents Myocardial Fibrosis via Decreasing Fraction of Colagen Type 1 Volume in Myocardial Tissue in Wistar Rats-Induced High Salt Intake
No full textMyocardial fibrosis is a pathological condition that responsible for initiation of heart failure. Neurohormonal endogen, angiotensin II, has a potential role to activate endothelin I, TGF-β1, myocardial fibroblast, extracelullar matrix deposition, structural changes and decreasing of cardiac function. Fibrotic process is also influenced by PPAR γ. Telmisartan has a potential effect to inactivate angiotensinergic system and to activate PPAR γ. It is expected that telmisartan has optimal effect to protect myocardial fibrosis. To know the role of variation dose of telmisartan to decrease collagen type 1 fraction volume in cardiac tissue of Wistar rats.
Ten-week-old male Wistar Rat (n = 30) were randomized into five groups, and each group consisted of 6 rats. Group 1 : negative control. Group 2 : rats were induced by intake Nacl 8% doses 2% body weight for eight weeks. Group 3 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 3 mg/kgBB for eight weeks. Group 4 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 6 mg/kgBB for eight weeks. Group 5 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 12 mg/kgBB for eight weeks. Collagen volume fraction was assessed by immunohistochemistry and ImageJ program. ANOVA test followed pos hoc test was used to analyzed each variable.
Collagen volume fraction significantly decreased in group 3, 4 and 5 compared in group 2. Telmisartan decreases collagen type 1 volume fraction of myocardial tissue
Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study
No full textoai:ojs2.bioscmed.com:article/5Introduction
Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa).
Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro.
MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20.
ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans.
ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity
The Efficacy of Temu Putih Fraction (Curcuma Zedoaria (Berg) Roscoe) Related Quality and Quantity of Spermatozoa in Male Wistar Rats
No full textBackground
Male participation in KB is still relatively low when compared to the participationof women. Researchers have to do research to find the contraception drug. Temu putih (Curcuma Zedoaria (Berg) Roscoe) is one of traditional herb that used as antifertility.
Aim of Study
Aim of this study to examine change in the amount, motility, morphology, and viability spermatozoa male rats (rattus norvegicus) due to temu putih fraction suplementation.
Methods
This study was an experimental study using a completely randomized design (CRD), post test with control group design. The sample in this study was 30 male rats, 10 weeks old, weight 150-200 gram. Rats were given temu putih fraction (n hexan, etylacetate and methanol-water) at dose of 300 mg/kgBB/day for 48 days. Temu putih was extracted by ethanol and did fractionation by liquid-liquid methods. The results of this study were assayed by SPSS 18.
Results
The amount, motility, morphology and viability of spermatozoa in the group of metanol fraction of water decreased compared with the control group (p= 0,000), motilitas of spermatozoa in the group of metanol water fraction decreased compared with the control group.
Conclusion
Temu putih fraction can reduce the amount, motility, morphology, and viability of spermatozoa in male rats.
Keywords: Fraction, Temu Putih, Amount of spermatozoa, Motility of spermatozoa, Viability Of Spermatozo
The Efficacy of Lerek Fruits (Phrynium maximum)Extract Related Body Weight, Lipid Profile and Leptin in Wistar Rats-Induced High Fat Diet
No full textBackground
Phrynium maximum (local name : lerek ) is a plant native to Sumatera, Malaya and Borneo. This plants have a potential as a local wisdom for therapeutics. In Sumatera, Lerek was used as traditional treatment for fever, diarrhea, diabetics and for wound healing.
Aim of Study
Aim of this study to explore the efficacy of lerek fruits extract in body weight, lipid profile and leptin level in Wistar Rats-Induced High Fat Diet
Methods
This study was an experimental study , pre-post test with control group design. The sample in this study was 30 male rats, 8 weeks old, weight 150-200 gram. Rats were given high fat diet and lerek fruits extract at dose of 50, 100 and 200 mg/kgBW/day for 2 weeks. Lerek fruits was extracted by infusion methods. The results of this study were assayed by SPSS 18.
Results
Lerek fruit extract 100 mg/kgBW was more potent to reduce body weight gain, triglyseride level and leptin level than lerek fruit extract 50 mg/kgBW, 200 mg.kgBW, negative control and positive control.
Conclusion
Lerek fruit extracts had a potention to reduce body weight, triglyceride and leptin level.
Keywords: Lerek fruit extract – body weight- triglyserida - lepti
The Relationships Between Single Nucleotide Polymorphisme (rs867500) STX1A Gene Exon 10 and Intelectual Intelligence
No full textBackground:
STX1A consists of 10 exons covering a genome overlay of 20.42 kb (2064 base mRNA) . The location of HPC-1 / Syntaxin 1A is on chromosome 7q11.23. Genes STX1A or HPC-1 or Syntaxin 1A are genes that encode membrane proteins that play an important role in neurotransmitter exocytosis in nerve cells.
Aim of Study:
The purpose of this study was to determine the relationship SNP (rs867500) in the gene STX1A Exon 10 with level intellect.
Method:
This research was observational analytic with cross sectional study design with a population of 368 and a sample of 36 were divided into two groups, namely the above-average IQ and IQ group average. To determine the relationship SNP (rs867500) STX1A gene Exon 10 with a level of intelligence used X2 test (Chi-square). Results:
The results showed not found SNPs in genes STX1A exon 10.
Conclusion :
STX1A Genes can be said to contribute to the collective intelligence of other genes, ie genes and gene IGFR2 FNBPL1 although there is no STX1A gene polymorphism in exon 10.
Keywords: Gene STX1A Exon 10, polimorphism, SNP, intelectual, intelligence