Bioscientia Medicina - Journal of Biomedicine and Translational Research
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Comparison of Phenolic Glycolipid 1 Antibody Levels in Household Contacts of Pausibasilary and Multibacilary Patients
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Background: Leprosy is a chronic infectious disease caused by Mycobacterium leprae (M. leprae). Distribution of leprosy cases in the world in 2015 was highest in the Southeast Asia region, with 156,118 cases. The increase in new cases and the high prevalence of leprosy requires action to break the chain of transmission. Household contact groups are easy to identify by the research group, because the group has good reasons for examining or getting chemoprophylaxis.
Objectives: Our objective was to compare Phenolic Glycolipid 1 (PGL-1) antibody levels in household contacts of pausibasilary (PB) and multibacilary (MB) patients.
Methods: This study was a analytic observational study with a cross-sectional design. ELISA examination will be conducted at the Leprosy Laboratory of the Institute of Tropical Diseases, Airlangga University, Surabaya. The study was conducted from 3 December 2019 to 31 January 2020, with a total sample of 60 samples.
Resuls: PGL-1 antibodies in MB leprosy household contact were greater than PGL-1 antibodies in PB leprosy household contact, and the results showed statistically significant (p = 0.011).
Conclusions: There are differences in the levels of PGL-1 antibodies between household contact PB leprosy patients and MB. The household contact PGL-1 antibody levels in MB leprosy are greater than PB leprosy
Functional Outcome After Neglected Injury In Pediatric Softball Players Measured By Quickdash Score
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Introduction : Pediatric softball players are prone to get injured as a result of high forces motions, with improper technique. Low communication and school-age often leads the injury became neglected. The aim of this study is to determine the incidence of Neglected injury in pediatric softball players in Bandung during a single season year of 2019. Method : The study was conducted in softball clubs in Bandung. The inclusion is the softball players aged 18 years old or below, grouped into; (1)never injured, (2)neglected injury by non-professional/traditional massage, and (3)untreated neglected injury/rest only. The samples were evaluated with the Quick Disabilities of the Arm Shoulder and Hand outcome questionnaire (QuickDASH). Data was analyzed with Anova test. Result : Among the 50 players, aged from 12 to 18 years old, 36 players (72%) were at group 1, 8 players (16%) were at group 2 and 6 players (12%) were at group 3. Most site of injuries for group 2 are at shoulder (62,5%) and group 3 are at elbow (66,6%). Significant differrences were identified between three groups with p=0.001. The highest quickDASH score are in group 2 (38,6), and the lowest are in group 1 (0,0). Conclussion : All of the quickDASH Score in this group of age are categorized in mild difficulty. Those whom treated by non-professional has lower functional outcome compared to never injured and untreated injured players
The Role Protein Sonic Hedgehog in Carcinoma Basal Cell
Get PDFA B S T R A C TIntroduction: Basal cell carcinoma (KSB) is a non-melanoma skin cancer (KKNM),which is most commonly found compared to other skin cancers. KSB originates fromstem cells in the bulk of hair follicles or inter-follicular epidermis, through the Sonichadgehog (SHH) activation pathway, an increase in Sonic hadgehog (SHH) proteinexpression, involving Patches protein (PTCH), smothened protein (SMO), in the formof increased protein transcription activation Glia (GLI) in the nucleus, binds to DNAto initiate tumor-aggressive growth and tissue. Objective: to determine therelationship between Sonic hadgehog (SHH) expression and non-aggressive andaggressive basal cell carcinoma. Methods: The study was carried out in anobservational laboratory with 35 primary KSB patients, the tissue was taken usingelliptic biopsy technique, made paraffin block specimens for histopathologicalexamination of the subtype of KSB consisting of 20 non-aggressive KSB patients,namely nodular and superficial KSB; 15 patients with aggressive KSB werepigmented KSB; Infiltrates KSB, micronodular KSB, metypical KSB (basosquamousKSB) and SHH immunohistochemical (CPI) examination using SHH antibodies, inthe Anatomy Pathology section, FK Unsri / RSMH Palembang. The characteristics ofKSB patients were recorded, namely sex, age, occupation based on the length ofexposure to BC, namely exposure <3 hours / day, exposure 3-6 hours / day,exposure ≥ 6 hours / day. The data were processed using the Statistical AnalysisSoftware Package (SPSS) version 20.0 (IBM Corporation), tested with Pearsoncorrelation test and chi square test and presented in the form of diagrams, andnarrative tables. Results: Pearson's test showed a significant correlation betweenthe clinical features of KSB and the histopathologic features of non-aggressive andaggressive types of KSB (p 0.020), there was a significant relationship between thesubtypes of histopathologic features of KSB with non-aggressive and aggressivetypes of KSB (p 0.000), there was a significant relationship between strong SHHexpression and BCC aggressive compared to non-aggressive KSB, p 0.000 (p <05α),and r = 732 Conclusion: There is a relationship between SHH expression and KSBaggressiveness. The increase in strong SHH expression shows the aggressiveness ofKSB, SHH expression can be used as a biological gene target both as a prognosticindicator and can be used as a target for treatment of aggressive KSB, especially inthe elderly
Correlation of Lymphocyte-Monocyte Ratio with Grading of Breast Cancer Malignancy
Get PDFIntroduction: Breast cancer cases are increase and fast becoming the leading cause of oncologic mortality among women worldwide. Delay in management will worsen the prognosis of breast cancer patients. The poor prognosis is correlate with high grade of breast cancer malignancy. Histology can be used as an indicator to assess the grade of breast cancer malignancy. In addition, a high predictive value of lymphocyte-monocyte ratio (LMR) has been suggested to indicate favorable prognosis of various cancer, and used as parameters for assessing breast cancer prognosis.
Method: This study is a quantitative research, using an observational approach. Laboratory examination results are collected from 43 breast cancer patients who will conduct treatment at Urip Sumoharjo Hospital from June to September 2019. Data are processed by the Spearman rank test using SPSS 22.0.
Results: Correlation test results between lymphocyte-monocyte ratio with histological grading of breast cancer obtained a P value of 0.376.
Conclusions: Lymphocytes to monocytes ratio (LMR) value cannot be utilize as a parameter for evaluating prognosis of breast cancer
The Efficacy and Safety of Plant Oil Mixtures in the Treatment of Xerosis with Pruritus in Elderly People: Randomized double blind Controlled Trial
Get PDFA B S T R A C TBackground: Xerosis skin and pruritus is the most commonly found in elderly withsymptoms mild to severe recurrent pruritus, the skin looks rough, scaly, fissured orcracked caused defects in the epidermal barrier function, increase co-morbidity aschronic infections and ulcers that are difficult to heal can decrease the quality of life.Virgin Coconut Oil (VCO) has been traditionally used for people in tropical regionas a moisturizer and anti-bacterial effect. In recent study that essential plant oilmixture have effect synergistically as anti inflammatory, anti pruritus, promotingwound healing may act promoting barrier skin and therapeutic. Objective: Efficacyand safety of plant oil mixtures as therapeutic and maintenance moisturizer.forXerosis and pruritus in elderly Method:A randomized controlled trial was involved61 elderly from nursing home and geriatric clinic in Moh. Hoesin General Hospital.They were randomized into 2 groups: plant oil mixtures (n=30) and Virgin Coconutoil (VCO) (n=31), applied on the volar legs twice a day for 4 weeks.Outcomes weremeasured at baseline and weekly, week 2 and 4.To measure of clinical dry scoreused ODSS; pruritus severity and side effect used VAS; for skin hydration usedcorneometer CM825and for sebum level used sebumeter SM 810. Results:Clinicaldry score, pruritus severity of plants oil mixtures group were significantly decreasedthan VCO at week 2 and 4 (p 0.000). Skin hydration and sebum level of plants oilmixture group were significantly increased than VCO at week 2 and 4 (p0.000).Clinical dry score, pruritus severity, skin hydration, andskin sebum levels ofplant oil mixtures were significantly better than VCO at week 4 (p 0.000). There isno side effects on both of plant oil mixtures and VCO groups Conclusion: Plant oilmixtures add pureCelin oil showed better efficacy as a therapeutin and maintanancemoisturizer for xerosis and pruritus in elderly. Both of plant oil mixtures and VCOcan be tolerated and safe to use
Potential of Sriwijaya Thermal Cycler Smart Controlling-Based as a Tool for DNA Sequence Polymerase Chain Reaction
Get PDFIntroduction. Along with the COVID-19 pandemic, the need for a thermal cyclerdevice for examining COVID-19 is getting bigger. Many laboratories have overusedthe use of thermal cyclers due to the limited availability of this tool. This study aimsto test the effectiveness of the Sriwijaya thermal cycler based on a smart controllerin conducting PCR DNA sequences. Methods. The research design was anexperimental study with a posttest control group approach, in order to see anoverview of the results of the polymerase chain reaction (PCR) in the form ofintermediate DNA bands using Sriwijaya Thermal Cycler compared to groups usingfactory-made Thermal cyclers. Results. The PCR results showed precise results fromthe image and band separation, and there was a clear separation of the bands onthe marker. The PCR results from the ACE I / D gene showed quite good and optimalresults in the separation of the PCR results band. Conclusion. Sriwijaya thermalcycler is effective in DNA polymerase chain reaction (PCR) sequences comparable tothat of the manufacturer's Thermal Cycler
The Effect of Ischemic-Reperfusion on Acute Kidney Injury Pathogenesis to the Glomerular Microscopic Appearance and Cystatin C Level in Wistar White Rat.
Get PDFAcute kidney injury (AKI) is a sudden decrease of kidney function. The incidence of AKI is increasing every year. One of the causes of AKI is the lack of blood supply to the kidneys (prerenal). At the present time, there are not many studies that report unilateral ischemic-reperfusion (UIR) duration which can cause kidney damage especially glomerulus. The aim of this study is to determine the effect of UIR duration to glomerular microscopic appearance, GFR and cystatin C produced in Wistar white rats. This study was performed in vivo by using Post-test Only Control Group Design. Unilateral Ischemic-Reperfusion was administered on the rat’s left kidney and recovery was done according to specified time. After recovery time, rat’s blood was taken, the rat was euthanized, and its kidneys were taken and stained with Picrosirius Red coloring. The kidneys were observed by using OptiLED and the photos were analyzed with ImageJ software. Blood samples were tested by ELISA to measure the cystatin C levels and the levels were converted into Larrson formula to obtain Glomerular Filtration Rate. The level of cystatin C increased along with the longer duration of UIR and compared inversely proportional to GFR which decreased along with the rise of UIR duration. Cystatin C and GFR had a significant mean difference (p<0.05) with all groups, except for the duration of the UIR group <60 minutes. The percentage of collagen obtained fluctuated but the whole group which was carried out by UIR had a significantly different collagen amount (p <0.05) with the sham-operated group. The average glomerular picture showed the addition of collagen, Bowman's capsule thickening and vascular retraction. The longer duration of UIR will worsen the kidney function.
Keywords: Unilateral Ischemic-Reperfusion, Cystatin C. Glomerular Filtration Rate, Collagen Area Fraction, Glomerulu
The Effect of Intravenous Vitamin C on Urine Neutrophil Gelatinase-Associated Lipocalin Among Septic Patients: A Randomised Control Trial
No full textBackground
The role of vitamin C to decrease organ dysfunction in sepsis was still controversial. This study aimed to explore the effect of intravenous (IV) vitamin C on urine NGAL (uNGAL) levels among septic patients in ICU.
Methods
This study was a randomized clinical trial held in Cipto Mangunkusumo Hospital from April to July 2019 with consecutive sampling method. Patients aged > 18 years with sepsis based on the criteria of sepsis-3 who were admitted to the ICU were included in this study. Exclusion criteria were those with chronic kidney problems, with kidney stones, undergo renal replacement therapy in the ICU. All subjects were divided into: Group A was treated with combination of vitamin C and thiamine while Group B was given thiamine only. The uNGAL level was measured at baseline, 24, 48 and 72 h after treatment. Anova for repeated measurement using General Linear Model for Repeated Measurement was used with level of significant at p-value <0.05.
Results
Total of 33 subjects were included. In Group A we found uNGAL (ng/ mL) were 74.5 (13.3-102.9), 77.3 (15.2-98.4), 67.2 (22.6-100.6), 77.2 (17.0-100.5) for baseline, 24 h, 48 h, and 72 h respectively. While in Group B uNGAL were 57.7 (11.5-94.5), 57.1 (6.4-97.7), 53.7 (13.3-99.6), 47.6 (4.5-100.9). No significant difference in terms of uNGAL between two groups at each hour was found.
Conclusions
This study showed that intravenous vitamin C administration had no effect on uNGAL among septic patients. Need more study to investigate approaches to improve kidney and inflammatory biomarker among septic patients
Association of Sociodemographic, Knowledge, Attitude and Practice with Pediculosis Capitis
No full textIntroduction : Pediculosis capitis is an infection of scalp and hair in humans caused by infestation of Pediculus humanus capitis and usually extends rapidly in a dense environment such as boarding schools. Aim of study : The aim of this study was to determine the association of students’ sociodemographic, knowledge, attitude and practice with pediculosis capitis in Pondok Pesantren Tahfidzil Qur'an Yayasan Tijarotal Lan Tabur Palembang.
Methods : This research was an analytical observational study with a cross sectional design conducted on November 2018. There were 117 samples that had the inclusion criteria and hadn’t exclusion criteria. Data collected from anamnesis, questionnaires and hair examinations of respondents. The results were analyzed using Chi-Square and Logistic Regression.
Results : Out of 117 students, 57 students (48.7%) has been found positive pediculosis capitis. Statistical test showed that age (p<0.001), gender (p<0.001), education level (p<0.001), knowledge (p=0.035), attitude (p=0.003) and practice (p =0.043) have significant association to the prevalence of pediculosis capitis. However, there was no significant association between father's education (p=1.000), mother's education (p=0.743), father's job (p=0.314), mother's job (p=1.000) and parents' income (p=1.000) to the prevalence of pediculosis capitis. The results of Multiple Logistic Regression analysis showed that gender, knowledge and practice were the most influential factors to the prevalence of pediculosis capitis (p<0.05) with the probability of 96.7%.
Conclusion : There was a significant association between age, gender, education level, knowledge, attitude and practice to the prevalence of pediculosis capitis, and the most dominant variables were gender, knowledge and practice
Association of Stress Level with Menstrual Disturbance Among Female Students in Medical Faculty Sriwijaya University
No full textAssociation Of Stress Level With Menstrual Cycle Disturbance Among Female Students In Medical Faculty Of Sriwijaya University. Menstruation occurs regularly every month will form a menstrual cycle. Normal menstrual cycles in women range from 21-35 days. However, the menstrual cycle is not always normal, many women experience menstrual disturbance. Menstrual cycle disturbance are caused by several factors, which is stress. This study aims to analyze the association of stress level with menstrual cycle disturbance among female students in Medical Faculty of Sriwijaya University. This study is analytical cross-sectional study with primary data from menstrual cycle, stress ISMA, personality type DSM V questionnaires.. In this study, 503 samples are fulfilled inclusion criteria. Among 503 subjects, 59 (11.7%) female students experienced polymenorrhea, 391 (77.7%) female students had normal menstrual cycles, 53 (10.6%) female students experienced olygomenorrhea. Chi Square test showed that there was an association between stress level and menstrual cycle disturbance. There is a significant association between between stress level and menstrual cycle disturbance among female students in Medical Faculty of Sriwijaya University.
Keywords: Stress level, Menstrual cycle disturbance