Bioscientia Medicina - Journal of Biomedicine and Translational Research
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The Influence of Knowledge, Attitudes and Behavior of Cleaning Officers with the Number of Mosquito Larvae in the Water Reservoir Punti Kayu Palembang City
No full textBackground Mosquito was one of the animals that can transmit deadly diseases.Information about water reservoirs as their habitat as well as human knowledge,attitudes and behavior factors are considered important to help eradicate mosquito.This study aims to determine the effect of knowledge, attitudes and behavior ofcleaners on the number of mosquito larvae in water reservoirs in Palembang.Methods This research was mixed method research (quantitative and qualitative) inthe field and laboratory. The research samples were all mosquito larvae andcontainers found. The research respondents were all cleaning services. Data werecollected by interviewing and filling in questionnaires, observing water reservoirsand identifying the types of mosquito larvae in microscope. Results A total of 40containers were found in Punti Kayu Nature Park. There were 8 containers that wereindoors (20%) and 32 containers were outdoors (80%) with 17 containers containedlarvae. Of all the containers that were positive for larvae, 6 were filled with clearwater and 11 were filled with turbid water. The number of larvae found were 358larvae outdoors and 82 larvae indoor with a total of 440 mosquito larvae with thegenus Culex spp. and Aedes albopictus species. The index of mosquito larvae wereobtained FLI (Free Larva index)=66,67%, HI (House Index)=33,33%, CI (ContainerIndex)=16,67%, BI (Breteau Index)=33.33% from the 3 buildings examined. Fromthese results, it was obtained DF (density figure) or density level of 5. There were 40containers that could be habitats for Culex spp. and Aedes albopictus. ConclusionThe knowledge, attitude and behavior of the cleaning services were good. Thenumber of mosquito larvae could be influenced by the frequency of cleaning and thelack of knowledge of the cleaning services
Methods of Selection and Maintenance of Experimental Animals for Biomedical Research
Get PDFA B S T R A C TResearch is an activity carried out based on scientific principles and methodssystematically to obtain information, data, and information from relatedsubjects, with understanding the theory and proving assumptions and / orhypotheses. The results obtained are conclusions that can be applied orbecome additional knowledge for the progress of science. However, researchactivities must still respect the rights and dignity of research subjects.Health research includes biomedical, epidemiological, social, and behavioralresearch. Some health research can be done in vitro, using mathematicalmodels, or computer simulations. If the research results are to be used forhumans, further research is needed using living materials (in vivo) such ascell lines and tissue cultures. However, to observe, study, and conclude alloccurrences in living things as a whole, experimental animals are neededbecause experimental animals have a value for each part of the body andthere are interactions between these body parts
The Ethics of Gene Modification: Considerations of Gene Editing and Genetic Selection
Get PDFThe field of hereditary qualities has offered ascend to innovation that will reform the natural sciences: the amazing quality altering device known as CRISPR-Cas9. Between clinical advancement, extreme new age solution for sicknesses and the danger of modifying human DNA in an irreversible manner, the lawful difficulties of applying CRISPR in the wellbeing setting, alongside the administrative and moral issues that may emerge should be drawn nearer with expanded duty. A primary objective or an assortment of purposes? Upgrade or quality altering to improve ordinary human characteristics, changes from altered genomes that may be acquired - repercussions of the new quality altering innovation could bring about adjusting human DNA. Current worldwide enactment and understanding, worldwide agreement worth seeking after regarding this matter and future guidelines required
Prescription Profile of Antibiotic Drugs Post Excision Biopsy of Oral Soft Tissue Disease in Palembang
Get PDFBackgroud An excisional biopsy is one of the surgical treatments given to cases ofsmall benign tumors in the soft tissues of the oral cavity. Antibiotics are prescribedby dentists as a follow-up therapy after excisional biopsy to prevent bacterialinfection. The purpose of this study was to determine the profile of antibiotic drugprescription after excision biopsy of oral soft tissue disease by dentists in Palembang.Methods This type of research is descriptive observational with a survey method.The research was carried out by distributing questionnaires to 40 dentists anddental specialists in Palembang who had performed excision biopsy treatment fororal soft tissue disease. Result The results showed that the antibiotic prescriptionof amoxicillin was 55%, the combination of amoxicillin and clavulanic acid was17.5%, and clindamycin was 12.5%. The cases found in this study were epulis57.5%, mucocele 30%, and traumatic fibroma 10%. Conclusion It can be concludedthat the antibiotic most often prescribed by dentists in Palembang after excisionbiopsy treatment was amoxicillin and the most common case was epulis
Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline
Get PDFELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model
Get PDFWound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14
Laboratory Examination of Syphilis
Get PDFSyphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal disease research laboratory (VDRL), toluidine red unheated serum test (TRUST), unheated serum reagin (USR), rapid plasma reagin (RPR) and treponemal serologic test, such as T. pallidum passive particle agglutination (TPPA), T. pallidum haemagglutination assay (TPHA), fluorescent treponemal antibody absorption (FTA-Abs), enzyme immunoassay (EIA) and rapid test. The algorithm of serologic test can be divided into traditional or reverse
Genital Herpes in Human Immunodeficiency Virus Infected Patients
Get PDFGenital herpes is a recurrent, lifelong sexual transmitted infection caused by HSV, especially type 2. Genital herpes is the most common infection in HIV patient. HSV-2 can increase the risk of HIV acquisition 2 to 3 times. Clinical manifestations of genital herpes can be different between HIV- infected and non-HIV patients. HIV-infected patients have a high risk of developing chronic and severe genital ulcers with atypical manifestation, prolonged healing, and resistant to treatment, depends on CD4 count. Genital herpes can be diagnosed based on history, clinical manifestation, laboratory and histopathologic examination. Management of genital herpes includes education and counseling patients and sexual partners, systemic antiviral, and symptomatic treatment
The Association between Urinary Tissue Inhibitor Metalloproteinase 2 (TIMP-2) and Insulin-like Growth Factor Binding Protein 7 (IGFBP-7) and Renal Recovery in Acute Kidney Injury
Get PDFBackground. Acute kidney injury (AKI) is a common and serious medical condition associated with significant increases in morbidity, mortality, cost of care and non recovery of kidney function that leads to progression to chronic kidney disease. Cell cycle arrest is implicated in the pathogenesis and repair process following AKI. The urinary cell-cycle arrest markers tissue inhibitor of metalloproteinase-2 (TIMP-2) and insulin-like growth factor binding protein 7 (IGFBP-7) have been utilized to predict the risk of AKI in many studies from specific population with good performance. However, their use in predicting recovery is still lacking. The aim of this study was to determine the association between two novel AKI biomarkers, urinary TIMP2 and IGFBP7 and renal recovery after 7 days of treatment in AKI patients at Dr. Mohammad Hoesin Hospital Palembang.
Method. This was a prospective cohort study conducted in dr. Mohammad Hoesin Hospital Palembang from January 2021 until March 2021. Subjects enrolled in this study were patients whom diagnosed AKI based on KDIGO 2012 criteria. Urine samples were collected upon patients’ enrollment within 24 hours of AKI diagnosis. We utilized Sandwich Enzyme Linked Immunosorbant Assay (ELISA) method to detect urinary TIMP-2 and IGFBP-7 levels. The primary outcome is recovery from AKI after 7 days of treatment. Chi square test is used to analyze the association between urinary TIMP-2 and IGFBP-7 levels and renal recovery.
Results. There were 70 subjects, only 22 of them were recovered after 7 days (31%). Median of urinary TIMP-2 and IGFBP-7 was 0,0047(0,0001-0,1439) [(ng/ml)2/1000]. There was significant association between urinary TIMP2 and IGFBP7 and renal recovery (p=0,027; OR 3,19; 95% CI 1,116-9,128).
Conclusion. There was significant association between urinary TIMP2 and IGFBP7 and renal recovery in AKI patients
Jugular Vein Aneurysm, When We Have to Do Surgery?
Get PDFIntroduction: The most common lesions of jugular vein dilatation are aneurysms and ectasia. A jugular vein aneurysm is less common compare to an arterial aneurysm in adults. Because of the rare incidence, treatment guidelines primarily associated with the timing of surgery are not clearly established. Proper treatment can reduce patient complaints without excessive intervention.
Case report: A 54 years old woman complained of swelling in the right neck that started three years ago and cephalgia for two years. From CT angiography, we obtained a jugular vein dilatation of 2.3 cm. During periodic evaluation from ultrasonography doppler, there is no increase in the size of the jugular vein.
Conclusion: Jugular vein aneurysm presenting in adults is an infrequent phenomenon. It is a benign condition, and conservative observation is advised. It should be operated only if symptomatic or progressive enlarging. A periodic examination must be done to evaluate the size of the jugular vein before a surgical decisio