Jurnal Fakultas Farmasi Umi (Universitas Muslim Indonesia)
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Antibacterial Evaluation of Agarwood (Aquilaria malaccensis) Leaf Ethanol Extract Against Pathogenic Bacteria Using TLC-Bioautography
Get PDFPathogenic bacteria are microorganisms that live in the human body that cause disease, such as the bacteria bacillus subtilis, Pseudomonas aeruginosa, and Eschericia coli. compounds such as flavanoid compounds, alcolloids, saponins, tannins, and terpenoids that has antibacterial properties The purpose of this research was to determine the antibacterial activity of agarwood leaf ethanol extract (Aquilaria malaccensis) against pathogenic bacteria by TLC - bioautography. The results shown that agarwood leaf ethanol extract (Aquilaria malaccensis) has potential as antibacterial against, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli, with three active spots identified in the bioautogram profiles an Rf value of 0.20 demonstrated inhibition against Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli Additionally, Rf values of 0.50 and 0.36 exhibited inhibitory effects against Pseudomonas aeruginosa and Escherichia coli, highlighting the potential of agarwood leaf ethanol extract in combating pathogenic bacteri
FORMULASI DAN EVALUASI PASTA GIGI YANG MENGANDUNG EKSTRAK DAUN TEH HIJAU (Camellia sinensis L.) DAN KULIT JERUK KEPROK (Citrus reticulata Blanco.)
Get PDFDental caries is one disease that occurs both in children and adults. One of the dental caries pathogenic microbial found in cariogenic biofilm or plaques is Streptococcus mutans. Natural material is one of the alternatives which can be used to treat the disease, some of them are green tea and tangerine peel. Both contain antibacterial active components against the Streptococcus mutans. This research used the ethanol extract of green tea and tangerine peel. Activity test of both extracts showed inhibition on Streptococcus mutans and Porphyromonas gingivalis 1.6%; 3.25% and 6.4% by green extract and 20%, 25% and 30% by tangerine peel. The goal of the research was to formulate the extract ethanol of green tea and tangerine peel into pharmaceutically stable toothpaste. Toothpaste is a semisolid disperse system preparations that contain amounts of insoluble solid. One of the functions of toothpaste is to clean the teeth. Physical stability test has been done to obtain the most optimum preparations. Formulation and evaluation of physical properties including organoleptic, homogeneity, pH measurement, viscosity, foam height and spreadability. Optimation base result showed that 1% Na CMC is the most stable in consistency. The formulation then continued with formulation into preparations and carried out the stability test and physical evaluations. Based on storage condition and physical test results showed that the preparations were stable pharmaceutically
Antibacterial Activity of Miana Leaf Ethanol Extract (Coleus scutellarioides (L) Against Some Pathogenic Bacteria Using The TLC Method - Bioautography
Get PDFMiana (Coleus scutellarioides (L.). is one of the plants that grows in areas with rather moist or slightly watery soil conditions, this plant is often consumed by people in Indonesia and is believed to be a medicinal plant. Empirically, miana leaves are used as antibiotics because they are known to have antibacterial activity. The purpose of this study was to determine the bioautogram profile of miana leaf ethanol extract against several pathogenic bacteria using the TLC-Bioautography method. As for the results of this study, ethanol extract of miana leaves (Coleus scutellarioides (L.)) has the potential as antibacterial against bacteria Staphylococcus aureus, Escherichia, Pseudomonas aeurigosa, and Streptococcus mutans, where for Staphylococcus aureus bacteria there are 2 active spots with Rf values of 0, 98, 0.32 and Streptococcus mutans bacteria there are 3 active spots with Rf values of 0.98, 0.76, and 0.69 respectively. As for Escherichia bacteria, there is 1 active spot with an Rf value of 0.81 and Pseudomonas aeurigosa bacteria there are 3 active spots, namely 0.87, 0.49, and 0.29, respectively
Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Water and Ethanol Extractsof Eleutherine palmifolia (L.) Merr. Against Staphylococcus aureus and Psedomonas aureginosa
Get PDFDayak onion bulbs (Eleutherine palmifolia (L.) Merr.) are indigenous herbal plants found in Indonesia, rich in phytochemical compounds such as alkaloids, glycosides, flavonoids, phenolics, steroids, and tannins. This study investigates the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of water and ethanol extracts of Dayak onion bulbs against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The water extract of Dayak onion bulbs was obtained by boiling using water, while the ethanol extract was obtained by maceration using 96% ethanol solvent. MIC and KBM were determined using the agar diffusion method. The results showed that water extract exhibited an MIC of 0.4% and MBC of 1.6% against both Staphylococcus aureus and Pseudomonas aeruginosa. Meanwhile, the ethanol extract demonstrated an MIC of 0.4% against Staphylococcus aureus and Pseudomonas aeruginosa bacteria with an MBC of 0.8% against Staphylococcus aureus bacteria and 1.6% against Pseudomonas aeruginosa bacteria. These findings suggest the potential of Dayak onion bulb extracts as antimicrobial agents against these pathogenic bacteria
Molecular Identification of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.) Using Polymerase Chain Reaction (Pcr)
Get PDFThe roots of the bidara plant have the potential as antibacterial agents. The antibacterial compounds in the roots of the bidara plant originate from secondary metabolites produced by endophytic compounds known as endophytic fungi. The purpose of this research is to identify the types of endophytic fungi in bidara roots. The identification of microorganisms can be done through morphology or molecular methods. However, morphological identification alone is unable to depict the morphospecies to the phylogenetic level of a microorganism, thus molecular identification using the Polymerase Chain Reaction (PCR) method is required. This study utilized root isolates from the bidara plant with the sample code IFAZ-6, which, after sequencing, yielded a pair of base pairs measuring 558bp. The results of BLAST and phylogenetic analysis revealed that the IFAZ-6 isolate has a close relationship with the species Clonostachys rosea., which has been recorded in the database with a 100% identity level
Antibacterial Activity of Endophytic Fungi Isolate from Bidara Plant (Ziziphus mauritiana Lam.) Against Bacteria That Cause Digestive Infections by Agar Diffusion Method
Get PDFIsolates of endophytic fungi coded IFAZ-6 derived from bidara plants (Ziziphus mauritiana Lam) has antibacterial abilities. This study determine whether those isolates has antibacterial activity against bacteria Escherichia coli. Isolates of endophytic fungi were purified and fermented for 21 days and the fermentation results were extracted using ethyl acetate solvent. The ethyl acetate extract obtained was carried out with a minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) as well as activity tests with the agar diffusion method. From the test results, statistical tests were carried out, the highest concentration IFAZ-6 isolate extracts that has highest inhibitory zone was 300,000 ppm in E. coli bacteria but only significantly different to 200.000 ppm
Optimization Time for Antibacterial Production of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.)
Get PDFResearch on optimization time for antibacterial production of endophytic fungal isolated from bidara root (Ziziphus mauritiana Lam.) has been conducted, with the aim of determining the optimum production time for antibacterial compounds. Endophytic fungi are a group of fungi that live within plant tissues without causing harm to their host plants. In this research, the roots of bidara were isolated and purified using PDA media, isolates were followed by screening endophytic fungi isolates against test bacteria. The results of the endophytic fungal isolation from the bidara roots yielded 10 endophytic fungal isolates. The screening test against the test bacteria showed the largest inhibitory zone for IFAZ-6. Subsequently, the growth curve of these isolates was determined through fermentation using MYB media using a shaker speed of 200 rpm at a temperature of 25-28°C for 27 days. Then, mycelia and supernatant were collected on days 11, 13, 15, 17, 19, 21, 23, 25, and 27 with a 48-hour interval. The mycelia were weighed until a constant weight was obtained, while the supernatant was used to test the activity against the test bacteria using MHA media. The growth curve results indicated a stationary phase on day 21. The antibacterial activity test showed that Isolate IFAZ-6 reached optimum activity on day 27 with an inhibitory zone of 12.33 mm against Salmonella thypi
Antibacterial Activity of Endophytic Fungi Isolated from Bidara Roots (Ziziphus mauritiana Lam.) Against Bacteria That Cause Skin Infections Using TLC-Bioutography
Get PDFOne common disease found in tropical countries is skin infection. Inappropriate use of antibiotics to treat skin infections can lead to bacterial resistance, making it necessary to explore the source of bioactive compounds in natural materials. The Bidara plant (Ziziphus mauritiana Lam.) is known to have antibacterial properties. This research aimed to determine the antibacterial activity of endophytic fungal isolated from the parts of the Bidara plant against bacteria that cause skin infections using the TLC-Bioautography method. In this research, the isolate with the code IFAZ-6 was purified to obtain a pure endophytic fungal isolate, and macroscopic and microscopic examinations were conducted on that isolate. The obtained isolate was fermented for 21 days at room temperature using MYB (Maltose Yeast Broth) medium. The fermentation products were extracted using ethyl acetate (1:1). The obtained extract was further tested using TLC with a chloroform: methanol eluent (7:1). The TLC-Bioautography results showed Rf values of 0.81 and 0.58 indicating antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Propionibacterium acnes bacteria. The identification of chemical components in the endophytic fungal isolate from the Bidara plant confirmed the presence of flavonoids, alkaloids, and anthraquinones. The extract from the endophytic fungal isolate with the code IFAZ-6 indicated antibacterial activity
