Jurnal Fakultas Farmasi Umi (Universitas Muslim Indonesia)
Not a member yet
463 research outputs found
Sort by
Potensi Antioksidan Daun Lobi-Lobi (Flacourtia inermis Roxb): Penentuan Kadar Total Flavonoid dan Fenolik
Full text linkFlavonoids and phenolics are natural phenolic compounds found in almost all plants, one of which is the batoko plum plant (Flacourtia inermis Roxb), which contains several phenolic compounds and has pharmacological activity. This plant is widely used, especially the fruit, by the people of Lampung province, but the leaves are still very rarely used. The use of this plant needs to be supported by research so that its use can be accounted for. The study used a maceration extraction method with 96% ethanol solvent. Determination of total flavonoid content of the extract used quercetin as the standard. Absorbance was measured using a UV-Vis spectrophotometer with a maximum wavelength of 433.0 nm. Determination of total phenolic content of the extract used a gallic acid standard. Absorbance was measured using a UV-Vis spectrophotometer with a maximum wavelength of 792.0 nm. In this study, the total flavonoid content in batoko plum leaf extract was calculated against quercetin compounds expressed in % QE and phenolics expressed in % GAE, with an average total flavonoid content of 0.0180 mg QE/g, and total phenolics of 0.0015 mg GAE/g. These results show that batoko plum leaves have potential as a source of antioxidants and as a raw material for herbal medicine
In Vitro, Efek Antidiabetes dan Antikolestrol Kopasanda (Chromolaena odorata L.)
Full text linkThe leaves of the kopasanda plant (Chromolaena odorata L.) contain alkaloids, flavonoids, saponins, terpenoids and tannins which have the potential to be anti-diabetic and anti-cholesterol. In vitro research has been carried out on the ethanol extract of kopasanda leaves (Chromolaena odorata L.) to determine the potential of α-glucosidase enzyme inhibitor compounds as antidiabetic by determining the IC50 value and the potential to reduce cholesterol levels as an anticholesterol by determining the EC50 value. Testing for the inhibitory activity of the α-glucosidase enzyme used the spectrophotometer method with a microplate reader at a wavelength of 405 nm, with acarbose as a comparison. The results showed that the activity of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) as an inhibitor of the α-glucosidase enzyme obtained an IC50 value of 444.019 µg/mL in the very weak category. In testing the anti-cholesterol potential using a UV-Vis spectrophotometer with measurements at a wavelength of 672 nm, the calculation results obtained an EC50 value of 1,017.118 µg/L. This shows that the activity of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) has the potential to inhibit cholesterol
Antibacterial Activity of Etanol Extract of Pinang (Areca catechu L.) Clup against Streptococcus mutans and Porphyromonas gingivalis
Full text linkAreca nut (Areca catechu L.) is a plant that has many uses and can be used for traditional medicine. Areca nut skin can be used to treat indigestion (dyspepsia), edema and beriberi due to low urine output. This study aims to determine the antibacterial activity of ethanol extract of areca nut peel against the test bacteria Streptococcus mutans and Porphyromonas gingivalis. In this research, the areca nut peel simplicia was extracted using the maceration method with 96% ethanol solvent, after which a liquid ethanol extract was obtained and then evaporated using a rotary vacuum evaporator to obtain a thick extract. After the extract is thick, a Minimum Inhibitor Concentration (MIC) test and a Minimum Bactericide Concentration (MBC) test are carried out. The results obtained in the Minimum Inhibitor Concentration (MIC) test and Minimum Bactericide Concentration (MBC) test on Streptococcus mutans and Porphyromonas gingivalis bacteria were 0.5%. Antibacterial activity test using the agar diffusion method was obtained, for Streptococcus mutans bacteria, the largest inhibitory zone diameter at a concentration of 16% was 12.58 mm and for Porphyromonas gingivalis bacteria at a concentration of 16%, namely 10.93 mm. Based on research, ethanol extract of areca nut peel has antibacterial potential
Formulasi dan Nilai SPF Sediaan Emulgel Kombinasi Ekstrak Etanol Daun Kelor (Moringa oleifera L.) dan Ekstrak Etanol Daun Kersen (Muntingia calabura L.)
Full text linkMoringa (Moringa oleifera L.) ethanol extract (EEDK) and kersen (Muntingia calabura L.) ethanol extract (EEDR) have strong antioxidant activity so they have the potential to be used as sunscreen preparations. Emulgel is a topical preparation that combines emulsion and gel so that it provides comfort when applied to the skin. The aim of the research is to formulate and determine the SPF (Sun Protection Factor) value of the emulgel preparation. Emulgel is formulated with EEDK, EEDR, carbopol 940, liquid paraffin, tween 80, span 80, propylene glycol, triethanolamine, methyl paraben, and distilled water. The preparation is made in 6 variations, namely a formula with a single active substance for F1 (EEDK 3%) and F2 (EEDR 2%) and a combination of both active substances for F3 (EEDK and EEDR 2%), F4 (EEDK 3% and EEDR 2%) and F5 (EEDK 2% and EEDR 3%) and F6 (no active substance). Evaluation of the preparation includes organoleptic tests, homogeneity, pH, spreadability, stickiness, viscosity, freeze thaw stability test, and determining the SPF value using a UV-Vis spectrophotometer. Based on the evaluation results, F4 is recommended and stability testing is continued. The results of the organoleptic evaluation showed that the emulgel was dark green in color, had a characteristic extract smell, and was homogeneous, the pH of the preparation was 6.32–5.82, the spreadability was 5.7–6.18 cm, the adhesive force was >4 seconds, and the viscosity was 2506 – 2313 cps. The SPF F1-F6 value was 8.52; 7.56; 10.19; 12.7; 10.62; and 1.09. Based on the research results, it was concluded that the F4 preparation was stable in storage and met all parameters and the SPF value of the preparation, namely 12.4 (maximum protection)
Isolation of Endophytic Fungus from Bay Leaves (Syzygium polyanthum Wight.) as Antibacterials Against Bacteria Causing Digestive Tract Infections by Bioautography-TLC
Full text linkSalam (Syzygium polyanthum (Wight) Walp), belonging to the Myrtaceae family, are noted for their potential antibacterial properties targeting bacteria linked to gastrointestinal infections. This study aims to identify endophytic fungus isolates from bay leaves that produce antibacterial compounds effective against bacteria responsible for gastrointestinal infections, utilizing TLC-Bioautography as a method of analysis. The endophytic fungus from bay leaves was isolated, yielding 17 pure isolates. The findings from the antagonist test of pure isolates of endophytic fungus revealed two isolates with the most significant inhibitory zone diameters: IFSP-8 and IFSP-15. These isolates demonstrated effectiveness against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae bacteria. The active isolate was then extracted by liquid-liquid extraction to obtain ethyl acetate extract for the IFSP-8 and IFSP-15 isolates. The antibacterial activity results of the ethyl acetate extract, analyzed through TLC-Bioautography with a chloroform: methanol (9:1) eluent, indicated the values of Rf1 0,76; Rf2 0,56; Rf3 0,47 for the IFSP-8 isolate. In contrast, the IFSP-15 isolate exhibited the values of Rf1 0,81; Rf2 0,69; Rf3 0,56, demonstrating inhibition of bacteria responsible for gastrointestinal infections
Antibacterial Activity of Ethanol Extract of Cocoa Leaves (Theobroma cacao L.) Against Gastrointestinal Infection Bacteria Tract Infection by Agar Diffusion Method
Full text linkThe cocoa plant (Theobroma cacao L.) leaves contain secondary metabolites, including flavonoids, saponins, and tannins, known for their potential antibacterial properties. This study aims to evaluate the antibacterial activity of the ethanol extract of cocoa leaves (Theobroma cacao L.) against bacteria that cause gastrointestinal infections using the agar diffusion method. The study assessed the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract. The results showed that the ethanol extract exhibited significant antibacterial activity, particularly at concentrations of 2.5%, 5%, and 10%, with the highest inhibition zone observed at 10% concentration. The extract was most effective against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae, with inhibition zones of 15.34 mm, 15.60 mm, 13.81 mm, and 16.43 mm, respectively. These findings suggest that cocoa leaf extract has the potential to be developed as an antibacterial agent against gastrointestinal pathogens
Antibacterial Activity Test of Ceremai Leaf Ethanol Extract (Phyllanthus acidus (L.) Skeels) Against Bacteria Causing Skin Infections by KLT-Bioautography and Agar Diffusion
Full text linkCeremai leaves (Phyllanthus acidus) are known for their medicinal properties, including the treatment of infectious diseases. This study aims to evaluate the antibacterial activity of ethanol extract from Ceremai leaves against skin infection-causing bacteria using Thin Layer Chromatography-Bioautography (TLC-Bioautography) and agar diffusion methods. The study began with an antibacterial screening test, followed by TLC-Bioautography, and then determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) at various concentrations (0.125% to 16%). The agar diffusion method was used to further assess antibacterial activity. Screening results showed antibacterial activity at a concentration of 1%. TLC-Bioautography with a chloroform: methanol: water (20:2:0.5) eluent revealed active spots with Rf values of 0.8, 0.54, 0.36, and 0.29. The MIC values were 0.25% for P. acnes and S. aureus, and 0.5% for P. aeruginosa and S. epidermidis. The MBC values were 0.5% for P. acnes and S. aureus, and 1% for P. aeruginosa and S. epidermidis. The largest inhibition zone in the agar diffusion test was observed at a 32% concentration against S. aureus, measuring 20.11 mm. These findings suggest that Ceremai leaf extract has significant antibacterial potential and could be considered for developing alternative treatments for skin infections
PENGARUH METODE EKSTRAKSI TERHADAP AKTIVITAS ANTIOKSIDAN DAUN KEDONDONG (Spondias dulcis) MENGGUNAKAN METODE DPPH & FIC
No full textSpondias dulcis plant is a tropical plant in the Anacardiaceae family. One of the plants from nature that has antioxidant properties. The aim of the research was to see the effect of variations in Spondias dulcis leaf extraction methods on the antioxidant activity of eliminating DPPH and FIC radicals. The % yield of various extracts from the maceration extraction method was 2.625%, UAE was 23.36% and digestion was 23.08%. screening of the phytochemical compound components of the ethanolic extract of Spondias dulcis leaves, the three variations of maceration extraction method, Ultrasonic (UAE) and digestion using thin layer chromatography using the eluent n-hexane: ethyl acetate (8:2) detected flavonoid and tannin content, while only the maceration extraction method was detected containing steroids, then the third alkaloid screening extraction variation was not detected. The conclusion is that the heating process in the digestive extraction method affects the antioxidant activity of the DPPH radical removal method of Spondias dulcis leaf extract, even though the percentage yield of extract from the digestive method is high (IC50 66,620 µg/mL) but the antioxidant activity is at a lower level and is classified as strong compared to extraction without maceration heating (IC50 38,979 µg/mL and UAE (IC50 29.838 µg/mL) are in the very strong category extraction and antioxidant activity of the FIC method in the ethanolic extract of Spondias dulcis leaves, the maceration extraction method IC50 342.72 µg/mL has a weak antioxidant activity category while the UAE extraction method (IC50 54.059 µg/mL) and digestion (IC50 83.093) are classified as strong. So the antioxidant activity method and DPPH reduction more effective than the Fe chelating method.
Antibacterial Activity of Ethanolic Extract of Pandanus Nut (Pandanus julianettii) Gastrointestinal Bacteria Using The Agar Diffusion Method
Full text linkGastrointestinal infections are a major global health concern, often caused by pathogenic bacteria. This study aimed to evaluate the antibacterial activity of the ethanolic extract of Pandanus nut (Pandanus julianettii) against gastrointestinal pathogens using the agar diffusion method. The Minimum Inhibitory Concentration (MIC) of the extract was determined to be 6.4% for Shigella dysenteriae, Salmonella typhi, and Escherichia coli, and 3.2% for Vibrio cholerae. The Minimum Bactericidal Concentration (MBC) for all tested bacteria was 12.8%. Antibacterial activity analysis revealed that at the 12.8% concentration, the largest inhibition zones were observed: 22.68 mm for Salmonella typhi, 22.20 mm for Shigella dysenteriae, 21.26 mm for Escherichia coli, and 20.80 mm for Vibrio cholerae. These findings indicate that the ethanolic extract of Pandanus julianettii possesses strong antibacterial properties and holds potential as a natural therapeutic agent for managing gastrointestinal infections