Jurnal Fakultas Farmasi Umi (Universitas Muslim Indonesia)
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In Silico Screening Inhibitors Histamine H2 Chemical Compounds in Licorice Plants (Glycyrrhiza glabra L.) Using Autodock Vina
Get PDFThe inquiry about on in silico screening of chemical compounds of Histamine H2 of Liquorice (Glycyrrhiza glabra L.), aimed to get the potential bioactive compounds found in plants The Liquorice (Glycyrrhiza glabra L.) as a potential inhibitor Histamine H2 with a screening in silico by Autodock Vina Docking process is carried out on enzyme Histamine H2 as receptors and 105 chemical compounds in plants Liquorice (Glycyrrhiza glabra L.) as ligands using Autodock Vina program. ∆Gbind value and lowest RMSD of each compound that has been in the docking taken the value of the free energy change (∆G) as a result of docking. Docking results showed that of the 105 chemical compounds of plant Liquorice (Glycyrrhiza glabra L.) are all potential as inhibitors Histamine H2 with free energy change (∆G) most low at Glabrene amounted to -9.6 kcal/mol, and the highest Isotachioside amounted to -4.5 kcal/mol
Potential of Extract of Tamarindus Indica L Leaves an Anti-Inflammatory on Carrageenan Induced Wistar Rats
Get PDFTamarind leaves are used in traditional medicine for inflammation, stomach pain, rheumatism, and sore throats. The aims of this study was to determined the potential of extract tamarind leaf as an anti-inflammatory in Wistar rats. This study was used experimental rats divided into 5 groups, namely group I was given Na. CMC, group II was given diclofenac sodium dose of 5.136 mg/kgBW, groups III, IV, and V were given ethanol extract of tamarind leaves at a doses of 250, 500 and 1000 mg/kgBW. The test animals induced with 1% lambda carrageenan by intraplantar after one hour given test preparation orally. Measurements of edema volume and thickness of the rats were carried out before and after induction for 7 hours at 1 hour intervals using a pletysnometer and calipers. The research data was calculated statistically using the one way anova test and Post Hoc LSD. The results of the study showed that the extract group at a dose of 1000 mg/kgBW had an anti-inflammatory effect not significantly different compared to the diclofenac sodium group at a dose of 5.136 mg/kgBW. Therefore, obtained results showed that the extract of tamarind leaf has an anti-inflammatory effect with an effective dose of 1000 mg/kgBW
IDENTIFICATION OF ENDOPYTIC FUNGI COMPOUND ON WHITE WEED LEAVES (Ageratum Conyzoides L.) CONTAINING THE POTENTIAL TO PRODUCE ANTIBIOTICS BY TLC-BIOAUTOGRAPHY.
Get PDFWhite weed (Ageratum conyzoides L.) is known to have antibacterial elements containing chemical compound such as saponin and flavonoids. This study aimed to examine the antibiotic activity of endophytic fungi isolates of white weed leaves by the TLC-Bioautography method. The results of endophytic fungi isolation of white weed leaves obtained 10 isolates consisting of IFDP 1, IFDP 2, IFDP 3, IFDP 4, IFDP 5, IFDP 6, IFDP 7, IFDP 8, IFDP 9, and IFDP 10. The results of the macroscopic examination of the ten isolates of endophytic fungi found different characteristics. The results of the screening using 9 samples obtained isolates that found activity showing high inhibitory power, such as IFDP 1, IFDP 2, and IFDP 4 isolates. Isolates of IFDP 1, IFDP 2 and IFDP 4 were fermented on MYB medium for 14 days. Then filtering and evaporation were carried out to produce an extract. Isolate fermentate extracts were identified using Thin Layer Chromatography with chloroform eluent: methanol (4:2). The antibiotic activity test was done using TLC-Bioautography method, and obtained an Rf value in isolate 1, at Rf 0.74 on Vibrio cholerae. Isolate 2 resulted rf value of 0.67 on Salmonella thypi, Vibrio cholerae, Eschericia coli, disentriae, Staphylococcus epidermis. Isolate 4 generated an Rf value of 0.74 on Pseudomonas aeruginosa and Vibrio cholerae. While the group of active chemical components contained in the endophytic fungi isolates of white weed leaves was lavonoids by using AlCl3 reagents, AICI3 reagent alkaloids, and Sulfuric acid for saponin identificatio
POTENSI EKSTRAK ETANOL BATANG WOLE WOE ASAL KABUPATEN HALMAHERA TENGAH SEBAGAI ANTIBAKTERI MENGGUNAKAN METODE KLT-BIOAUTOGRAFI
Get PDFThe development of natural materials both from plants and from other materials as traditional medicines can be done by taking habits from the community in utilizing natural materials empirically. The wole woe plant is one of the plants that comes from the forest and grows wildly and is empirically used as a traditional medicine by the Weda community in Central Halmahera Regency. This study aims to see the chromatogram of wole woe stem extract and the group of chemical components that have potential as antibacterial using KLT-Bioautography method. The results of the chromatogram of the ethanol extract of wole woe stem, obtained 6 spots and there are 2 spots with rf values of 0.65 and 0.09 which have activity against bacteria Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis and Pseudomonas aeruginosa. The results of the identification of chemical components using specific reagents, obtained chemical groups of anthraquinones, flavonoids, phenolics and alkaloids have potential as antibacterial, so that the ethanol extract of wole woe stem has potential as antibacterial
AKTIVITAS ANTIBAKTERI FERMENTAT FUNGI ENDOFIT DAUN KASUMBA TURATE (Carthamus tinctorius L.) ASAL GALESONG TERHADAP BAKTERI UJI PENYEBAB INFEKSI KULIT
Get PDFKasumba turate (Carthamus tinctorius L.) contains phenolic compounds of flavonoids and carotenoids which have antioxidant, antibacterial, antiviral, anti-inflammatory, antiallergic and anticancer activities. This study aims to obtain isolates of endophytic fungi that have potential as antibacterial using the TLC-Bioautography method. This research was conducted by isolating endophytic fungi from the leaves of kasumba turate (Carthamus tinctorius L.) and after that purification and macroscopic tests were carried out and eight pure isolates were obtained. The results of the isolate screening tests with IFDKT 5 and IFDKT 6 codes showed the highest inhibition. Then tested the antibacterial activity by TLC-Bioautography. The isolates with IFDKT code 5 showed five spots were active against Staphylococcus aureus and Pseudomonas aeruginosa (Rf1 0.83; Rf2 0.65; Rf3 0.47; Rf4 0.30; Rf5 0.07), and six spots were active against bacteria Staphylococcus epidermidis (Rf1 0.94; Rf2 0.83; Rf3 0.65; Rf4 0.47; Rf5 0.30, Rf6 0.07). Then, the isolates with code IFDKT 6 showed four active spots against Staphylococcus aureus and Pseudomonas aeruginosa (Rf1 0.80; Rf2 0.58; Rf3 0.40; Rf4 0.10), and five spots were active against Staphylococcus epidermidis (Rf1 0.96; Rf2 0.80; Rf3 0.58; Rf4 0 .40; Rf5 0.10). Therefore, fermentate isolate of the endophytic fungus of kasumba turate leaves (Carthamus tinctorius L.) has the potential to inhibit Staphylococcus aureus, Stahpyloccous epidermidis and Pseudomonas aeruginosa bacteria that cause skin infection
UJI AKTIVITAS ANTIOKSIDAN ETANOL BUAH TIN (Ficus carica) DENGAN METODE DPPH DAN FRAP
Get PDFIndonesia as a developing country has limitations in overcoming health problems. Based on the 2018 National Basic Health Research (Riskesdas), the prevalence of degenerative diseases is increasing. The main cause of death was stroke (15.4%) followed by hypertension, diabetes mellitus, and tumors. One of the causes of this degenerative disease is oxidative stress, namely the large number of oxidants in the body through an oxidation process. Antioxidants are compounds that prevent or postpone oxidation reaction of a molecule by inhibiting initiation or propagation process of the chain oxidation reaction The chemical structure of antioxidants, free radical sources, and physico-chemical properties of different sample preparations can provide various antioxidant activity test results. Therefore, a selective antioxidant activity analysis method is needed for a certain type of sample. The purpose of this study was to test the antioxidant activity of the ethanol extract of fig fruit using the DPPH and FRAP. Methods This research was conducted using the antioxidant activity test method DPPH and FRAP. Based on the research results of antioxidant activity using the DPPH method, the IC50 value of the ethanol extract of fig fruit was 121.74 μg/mL and the FRAP method obtained a value of 11.11 mgAAE/g extrac
PENGARUH SUHU PENYIMPANAN TERHADAP SIFAT FISIK TABLET EFFERVESCENT EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) DAN BUAH LEMON (Citrus limon L.)
Get PDFEffervescent tablet is a dosage form that can produce bubbles originating from an acid and base reaction that forms a gas. The reaction between acids and bases in effervescent tablets is very sensitive to temperature if stored continuously. So that testing for drug stability is very important in evaluating drugs, one of which is by knowing the effect of temperature on drug stability, especially in the form of effervescent tablets. This study aims to determine the effect of storage temperature on the physical properties of effervescent tablets made from red dragon fruit peel extract (Hylocereus polyrhizus) combined with lemon fruit powder (Citrus limon L.). The research method was carried out by storing effervescent tablets at cold temperature (±120C), room temperature (±250C), and warm temperature (±400C). And observed for 1 month with testing every 1 week. The results of this study are storage temperature can affect the physical properties of effervescent tablets made from red dragon fruit peel extract (Hylocereus polyrhizus) with a combination of lemon fruit powder (Citrus limon L.). The best temperature is cold temperature around 120C because at that temperature the acid and base reactions in effervescent tablets are not excessive which results in effervescent tablets still in a stable conditio
Uji Aktivitas Penghambatan Dipeptidyl peptidase-4 (DPP-4) Senyawa dari Lima Tanaman Indonesia secara In-silico
Get PDFABSTRAKTeknik komputasi virtual screening dapat digunakan untuk memprediksi dan menjelaskan aktivitas senyawa apa yang berperan dalam penghambatan DPP-4. Lima tanaman Indonesia seperti Caesalpinia sappan, Mutingia calabura, Rheum palmatum, Ficus religiosa dan Tinospora crispa telah terbukti memiliki aktivitas penghambat dipeptidyl-peptidase-4 (DPP-4) secara in-vitro akan tetapi belum ada penjelasan senyawa apa yang berperan terhadap aktivitas tersebut. Penelitian ini bertujuan untuk memprediksi dan menjelaskan senyawa-senyawa dari lima tanaman yang memiliki aktivitas penghambatan DPP-4 secara In-silico. Metode docking menggunakan perangkat lunak Molegro Virtual Docker dengan 16 protokol kombinasi pencarian algortima dan fungsi nilai. Sebelum Docking dilakukan validasi metode dengan cara redocking native ligand protein penghambat DPP-4 dengan kode PDB 3G0B, kemudian dilanjutkan docking secara bersamaan 151 senyawa dari lima tanaman Indonesia dan 1 ligan standar alogliptin dengan reseptor dipeptidy lpeptidase-4 (kode PDB: 3G0B) dengan metode docking yang valid. Hasil validasi metode menunjukkan 16 protokol kombinasi memberikan nilai RMSD < 1 Å. Hasil docking berdasarkan rerank score paling rendah dan kemiripan interaksi residu asam amino dibandingkan ligan standar Alogliptin menunjukkan tiga senyawa 6'-O-Lactoyl Borapetoside B (rerank score -99.78 kcal/mol) dan Borapoteside A (rerank score -98.76 kcal/mol) dari tanaman Tinospora crispa dan Rhaponticin (rerank score -99.37) dari tanaman Rheum Palmatum sedangkan ligan standar Alogliptin memiliki rerank score -93.14 kcal/mol dengan interaksi residu asam amino Glu206, Glu206, Tyr547, Tyr632. Metode docking dengan mvd terbukti valid dan Physcion 8-gentiobioside, Luteolin 4'-metil eter 7-glukosida, 6'-O-Lactoyl borapetoside B, Rhaponticin dan Tinocrispol A diprediksi memiliki aktivitas penghambatan DPP-4.Kata Kunci: docking, penghambat DPP-4, Tinospora crispa, Rheum palmatu
