Jurnal Fakultas Farmasi Umi (Universitas Muslim Indonesia)
Not a member yet
463 research outputs found
Sort by
TLC Bioautography Analysis of Sappan (Caesalpinia sappan L.) Wood Extract Against Propionibacterium acnes and Staphylococcus epidermidis Bacteria
Get PDFSappan wood (Caesalpinia sappan L.) is one of the plants that is widely used in traditional medicine in Indonesia. Sappan wood is known to have several benefits, including its ability to treat several infectious diseases caused by bacteria. The aim of this research is to determine the class of active compounds that have antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis using TLC Bioautography analysis. The TLC results showed that the ethanol extract of Sappan wood contains alkaloid, flavonoid and phenol compounds. Bioautography TLC results show that the ethanol extract of Sappan wood has the potential for good antibacterial activity against the bacteria Propionibacterium acnes and Staphylococcus epidermidis with Rf values of 1: 0.92, Rf 2: 0.78, Rf 3: 0.65, Rf 4: 0.50, Rf 5: 0.38, Rf 6: 0.23 and Rf 7: 0.09 for Propinibacterium acnes. And the values of Rf 1: 0.65, Rf 2: 0.50, Rf 3: 0.38, Rf 4: 0.23 and Rf 5: 0.09 for Staphylococcus epidermidis
UJI TOKSISITAS SUBKRONIS EKSTRAK ETANOL DAUN MENGKUDU (Morinda citrifolia L.) DENGAN PENETAPAN KADAR KREATININ DAN BUN TIKUS PUTIH
Get PDFThe subchronic toxicity test of Noni (Morinda citrifolia L.) ethanol leaf extract have been studied. The purpose of this study was to determine the effect of subchronic toxicity of noni ethanol leaf extract (Morinda citrifolia L.) on increased levels of creatinine and BUN (Blood Urea Nitrogen) of white rats. Noni leaves were extracted by using the maceration method with 96% ethanol dissolution for 3 times 24 hours while occasionally stirring until a thick extract was obtained. Then the blood creatinine and BUN levels were determined before administering the test preparation and on day 28. The research was carried out for 28 days then continued for 14 days for the satellite group. The results of the subchronic toxicity testing that was carried out showed a double increase in creatinine and BUN levels compared to normal values. Noni leaf extract treatment caused an increase in creatinine and BUN levels in the subchronic toxicity test at doses of 250, 500 and 1000 mg/kg BW, and the highest increase in levels occurred in the 1000 mg/kg BW grou
PENGARUH INTERVENSI EDUKASI BERBASIS APLIKASI PONSEL PINTAR TERHADAP TINGKAT KUALITAS HIDUP PENDERITA DIABETES MELITUS TIPE 2
Get PDFPoor quality of life is one of the leading causes of death in individuals with diabetes mellitus, so people with diabetes need to be educated about the disease to have a decent quality of life and avoid complications. Today's technological advances have resulted in the emergence of many new innovations, including in the health sector, such as the use of smartphone apps for patient education. This study seeks to describe the quality of life of type 2 diabetes mellitus patients in the outpatient installation at PKU Muhammadiyah Gamping Hospital and to ascertain whether there are any differences between the quality of life of these patientss' prior to and following the administration of educational interventions based on smartphone applications. This study falls under the category of pre-experimental research, with a one-group pretest and posttest design. Respondents for this study were recruited using the quota sampling approach, which yielded up to 50 participants based on the research requirements. This study was conducted at the PKU Muhammadiyah Gamping Hospital outpatient facility from October to December 2022. The Diabetes Quality of Life (DQoL) questionnaire was used to collect research data before and after the smartphone application-based educational intervention "Diary Diabetes" was given to respondents. According to the findings of this study's descriptive statistical analysis, out of 50 respondents, 37 (or 74%) had high levels of life satisfaction. The study's findings also revealed an increase in the average, median, maximum, and standard deviation of the respondents. According to the hypothesis test, the study's paired sample t- test had a significance value of 0.003, indicating that there was a difference between the patients' quality of life before and after receiving smartphone application-based educational interventions for type 2 diabetes mellitus in the outpatient facility of PKU Muhammadiyah Gamping Hospital
ISOLATION OF ENDOPHYTIC FUNGI FROM PATCHOULI LEAVES (POGOSTEMON CABLIN BENTH) AS ANTIBACTERIAL AGAINST PATHOGENIC BACTERIA BY BIOAUTOGRAPHY AND AGAR DIFFUSION
Get PDFPatchouli (Pogostemon cablin Benth) is a plant used as a substance in the pharmaceutical industry such as the manufacture of anti-inflammatory, antifungal, anti-insect and anti-inflammatory drugs. This study aimed to obtain endophytic fungi isolates having the potential as antibacterial by using TLC- Bioautography and agar diffusion methods. This research was conducted by isolating the endophytic fungi from Patchouli leaves and after that, purification and macroscopic tests were carried out and ten isolates were obtained pure. The results of the isolate screening test with codes IFDN 1, IFDN 3, IFDN 4, IFDN 6, IFDN 7, IFDN 8 and IFDN 9, showed the highest inhibition. Furthermore, the antibacterial activity was assayed by TLC-Bioautography. The results showed active spots (Rf 3 = 0.54; Rf 4 = 0.61; Rf 6 = 0.58; Rf 7 = 0.45; Rf 8 = 0.54; Rf 9 =0.47) against Eschericia coli, Staphylococcus aureus and Staphylococcus epidemics. The results of the antibacterial activity test by diffusion method obtained an inhibition zone for each bacterium, namely Staphylococcus aureus of 8.66 mm, Staphylococcus epidermidis of 8.38 mm, and Eschericia coli of12.23 mm.Key Words: Agar diffusion; Antibacterial; Endophytic fungi; skin infections; TLC Bioautograph.
Pengaruh Metode Ekstraksi Terhadap Senyawa Flavonoid Total Ekstrak Etanol Daun Patikan Kebo (Ephorbia hirta)
Get PDFEuphorbia (Euphorbia hirta) merupakan tanaman yang banyak digunakan dalam pengobatan tradisional. Salah satu senyawa aktif yang terdapat pada ekstrak etanol daun Euphorbia adalah flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh variasi metode ekstraksi terhadap kandungan flavonoid total pada ekstrak daun Euphorbia. Ekstraksi sampel menggunakan metode perkolasi dan refluks dengan nilai rendemen untuk metode perkolasi sebesar 4,3% dan metode refluks sebesar 5,3%. Analisis kualitatif sampel menggunakan Kromatografi Lapis Tipis (KLT). Penentuan kadar flavonoid total ekstrak etanol daun Euphorbia diperolehdari data spektrofotometer UV-Vis. Panjang gelombang maksimum yang digunakan adalah 429 nm. Hasil penelitian menunjukkan bahwa kandungan total flavonoid ekstrak etanol daun Euphorbia untuk metode perkolasi adalah 2,32% dan untuk metode refluks adalah 3,21%
IDENTIFICATION OF ACTIVE CHEMICAL COMPONENTS OF ETHANOL EXTRACT OF PAINTED NETTLE LEAVES (COLEUS SCUTELLARIOIDES L. BENTH) AGAINST GASTROINTESTINAL INFECTION BACTERIA USING TLCBIOAUTOGRAPHY AND AGAR DIFFUSION METHOD
Get PDFInfectious diseases are one of the public health problems caused by the transmission and multiplication of microorganisms. The research on the antibacterial activity of painted nettle leaves (Coleus scutellarioides L. Benth) extracts was carriedout to examine the activity and chemical components that provide inhibition against gastrointestinal infection bacteria. The extraction of painted nettles leaves performed maceration methd using a solvent of 96%. The extraction results were examined to determine the antibacterial activity at 1% concentration of the test bacteria: Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The results showed that 96% ethanol extract of painted nettle leaves inhibited Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The research conducted using agar diffusion indicated that painted nettleleaf extract showed activity against bacteria and had highly strong potential with diameters of 6-20 mm. The results of Thin Layer Chromatography (TLC) Bioautography test of ethanol extract of painted nettle leaves using chloroform:methanol eluent (3:1) showed inhibition of Escherichia coli, Shigella dysenteriae, and Salmonella thypi at Rf values of 0.90, 0.80, 0.56. Therefore, the identification test confirmed that the chemical componentsproviding antibacterial are from flavonoids, phenols, terpenoids, alkaloids, and steroidsKey words: Identification of chemical components, painted nettle leaves, gastrointestinal bacteria, TLC-Bioautography and Agar diffusio
Studi Penambatan Molekuler Senyawa Tectoquinone Terhadap Enzim α-Glukosidase
Get PDFThe investigation focused on conducting an in silico screening of chemical compounds isolated from Syzygium oblanceolatum (C.B.Rob) to identify potential bioactive compounds that could act as inhibitors of α-Glucosidase. This screening involved tectoquinone as the potential inhibitor for α-Glucosidase and employed the Autodock Vina Docking process. The target enzyme, α-Glucosidase, was used as the receptor with 20 binding sites on the enzyme receptor 1LWJ and the Autodock Vina program was utilized for this purpose. The values of ∆Gbind and the lowest RMSD were determined for each of the 20 targeted binding sites on 1LWJ, representing the free energy change (∆G) resulting from the docking. The docking results demonstrated that the free energy change (∆G) for the 20 targeted binding sites ranged from -6.346 kcal/mol to -9.720 kcal/mol. The most favorable free energy change (∆G) was observed at the 16th binding site with a value of -9.720 kcal/mol, while the highest was recorded at the 18th binding site with a value of -6.346 kcal/mol
Studi Komparasi Aktivitas Antiradikal Bebas Ekstrak Metanol Kulit Buah Pisang Ambon (Musa acuminata Colla) Muda dan Matang dengan Metode DPPH
Get PDFAmbonese banana fruit peel has antioxidant, antimicrobial, and anti-inflammatory activities. This search aims to investigate the potential of free radical scavengers based on the binding of DPPH free radicals (1,1-Diphenyl-2-picryl Hidrazil). Ambonese banana fruit peel was extracted by maceration using methanol. Free radical activity assay on sample measured by inhibition of DPPH using spectrophotometer UV-Vis at a wavelength at 516 nm. Identification by the TLC method showed that the peel extract of Ambonese banana was active as free radical scavengers where the DPPH solution changed in color from purple to yellow after being sprayed. The calculation results in IC50 values of each sample methanol extract of unripe Ambonese banana fruit peel is 11.78 µg/mL, while the methanol extract of the ripe Ambonese banana fruit peel is 151.56 µg/mL. The standard quercetin has IC50 values of 4.44 µg/mL. The result showed that the methanol extract from the Ambonese banana fruit peel (Musa acuminata Colla) has antioxidantactivity