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    Assessing genetic diversity of Tecomella undulata (Sm) - an endangered tree species using amplified fragment length polymorphisms-based molecular markers

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    Tecomella undulata is an important agro-forestry tree in the western parts of India, which has been included in the list of endangered plant species due to over exploitation. There is an urgent need to formulate appropriate conservation and breeding strategies to save this versatile tree. Quantification of genetic diversity in the existing populations of T. undulata prevalent in India, is an essential first step towards this objective. The present work was undertaken to investigate the utility of amplified fragment length polymorphism (AFLP) markers in the assessment of genetic diversity and phenetic relationship among 42 T. undulata plants collected from different regions of India. Eight AFLP primer combinations generated a total of 338 bands across the 42 T. undulata accessions screened. On an average, 42 AFLP fragments were amplified per reaction. Of the total 338 AFLP fragments, 71.7% was polymorphic across all the accessions. From the AFLP data, a similarity matrix based on Jaccard's coefficient was calculated which was used for constructing a phenetic dendrogram using UPGMA analysis. T. undulata accessions represented high levels of genetic variation as indicated by GS values ranging from 0.721 to 0.83, at which 42 T. undulata accessions grouped together. The pattern of grouping in the phenogram was highly consistent as indicated by high values of cophenetic correlation (r = 0.87, P = 1) as also by high bootstrap values at the key nodes. Principal correspondence analysis also Cited By since 1996 This article has been cited 0 times in Scopus. Inform me when this document is cited in Scopus: E-mail Alert Find related documents In Scopus based on On the Web based on title Document Type: Article View references (31) references authors keywords authors keywords Page 1 of 5 Scopus - Current Science: Assessing genetic diversity of Tecomella undulata (Sm.) - An endangered... 02/04/2008 http://www.scopus.com/scopus/record/display.url?view=basic&eid=2-s2.0-3 revealed that T. undulata accessions were scattered on the plot. Based on our study, it may be concluded that there is a high level of genetic diversity in T. undulata accessions analysed, which may be attributed to its out-crossing nature. Our study may be useful in identifying diverse genetic stocks of T. undulata, which may then be conserved on priority basis. Author Keywords AFLP; Conservation; Genetic diversity; Molecular makers; Tecomella undulata References (31) view in table layout Select: Page 1. (1982) The Wealth of India, 10, pp. 134-136. Publications and Information Directorate, CSIR, New Delhi, Anon 2. Jindal, S.K., Kackar, N.L., Solanki, K.R. Evaluation of Tecomella undulata (Sm.) SEEM germplasm from western Rajasthan in situ (1985) Trans. Indian Soc. Desert Technol. Univ. Cent. Desert Stud, 10, pp. 33-37. 3. Jindal, S.K., Solanki, K.R., Kackar, N.L. Phenology and breeding systems of Rohida (Tecomella undulata (Sm.) SEEM) (1985) Indian J. For, 8, pp. 317-320. 4. Jindal, S.K., Kackar, N.L., Solanki, K.R. Germplasm collection and genetic variability in Rohida (Tecomella undulata (Sm.) Seem) in western Rajasthan (1987) Indian J. For, 10, pp. 52-55. 5. Rao, A., Kiran-Bala, V., Lahiri, A.N., Bala, K. Influence of trees on microorganisms of aridisol and its fertility (1989) Indian For, 115, pp. 680-683. 6. Azam, M.M. Anti-HIV agents and other compounds from Tecomella undulata (1999) Orient. J. Chem, 15, pp. 375-377. Cited 2 times. 7. Dushyent, G., Bohra, A. Toxic effects of various plant part extracts on the causal organism of typhoid fever (2000) Curr. Sci, 78, pp. 780-781. Cited 4 times. 8. Ahmad, F., Alam Khan, R., Rasheed, S. Preliminary screening of methanolic extracts of Celastrus paniculatus and Tecomella undulata for analgesic and antiinflammatory activities (1994) Journal of Ethnopharmacology, 42 (3), pp. 193-198. Cited 12 times. doi: 10.1016/0378-8741(94)90085-X 9. Jain, S.K., Rao, R.R. An assessment of threatened plants of India (1983) Botanical Survey of India, Howrah, p. 334. Page 2 of 5 Scopus - Cur

    Signal Durations and local Richter magnitudes in northeast india: An empirical approach

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    Twenty four analog seismic stations are operated by the Regional Research Laboratory (Jorhat), National Geophysical Research Institute (Hyderabad) and by the India Meteorological Department (IMD) in the Northeastern region (NER) of India. 8000 seismograms of 1992 shallow (5-30km) earthquakes recorded by these stations during the period from January 1985 to December 1999, have been used to establish relationships between signal durations and the local Richter magnitudes (ML). In order to obtain the empirical relations for the determination of duration magnitudes (MD), signal duration estimates have been fitted using regression analysis to models of the form Model-I: MD = C0 + C1 Log10 (S.D) + C2 Δ + C3 h Model-II: MD = C0 + C1 Log10 (S.D) + C2 Δ + C3 h + C4 [Log10 (S.D)] (S.D)2, where S.D is the signal duration in seconds, Δ epicentral distance in degree and h focal depth in km. The models yielded duration magnitudes at each of the 24 stations having standard deviations as low as 0.07. For these stations, station factors are obtained by finding the average of the deviations of network magnitude (i.e. mean estimate of station magnitudes for each earthquake, denoted by MD A) from station magnitudes (MD) for the earthquake events in NER. Over - and under - estimations of station magnitudes with respect to ML are also determined for each station. It has been observed that the estimates of MD (A) scatter up to about 0.8units with respect to ML for both the models. Application of these factors reduced scatter down to ± 0.25 units for both the models. © Geol. Soc. India

    Dicarbonylrhodium(I) complexes of functionalized pyridine ligands and their catalytic activities

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    Reactions of dimeric complex [Rh(CO)2Cl]2 (1) with pyridine ester ligands methyl picolinate (a), methyl nicotinate (b), methyl isonicotinate (c),ethylpicolinate(d),ethylnicotinate(e)andethylisonicotinate(f)inthe1:2molarratioaffordthecomplexesofthetype[Rh(CO)2ClL](1a–f). Thecomplexes1a–fexhibittwoequallyintenseν(CO)bandsintherange1990–2091cm−1 indicatingcis-dispositionofthetwoterminalcarbonyl groups.Thecomplexes1aand1dundergopartialdecarbonylationreactioninsolutiontogivethecorrespondingchelatedmonocarbonylcomplexes [Rh(CO)Cl(methylpicolinate)](1a)and[Rh(CO)Cl(ethylpicolinate)](1d),respectively.Thecomplexes1a–fundergooxidativeadditionreaction withdifferenttypesofelectrophileslikeCH3I,C2H5I,C6H5CH2ClandI2 toyield[Rh(CO)(COCH3)ClIL](2a–f),[Rh(CO)(COC2H5)ClIL](3a–f), [Rh(CO)(COCH2C6H5)Cl2L] (4a–f) and [Rh(CO)ClI2L] (5a–f) complexes, respectively. The complexes have been characterized by elemental analysis,IRand 1HNMRspectroscopy.Thetimetakenbythedifferentcomplexes1a–fforthecompletionofoxidativeadditionreactionsofCH3I are different and the complex 1f took the shortest time while the complex 1b required the longest time. The catalytic activity of the complexes [Rh(CO)2ClL] (1) in carbonylation of methanol is higher (TON=844–1251) than the well known [Rh(CO)2I2]− species (TON=653). © 2006 Elsevier B.V. All rights reserved

    Effect of bioformulation in reclamation of crude oil contaminated soil and determination of metal contents in plants grown after reclamation

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    Crude oil is of organic origin and is an extremely complex structure of hydrocarbons. Besides carbon, hydrogen, nitrogen and sulphur many other elements are present in crude oil. In oil fields, during drilling, gathering, transporting or in some other operations, some quantity of the crude oil gets spilled over the neighboring areas. The spilled crude oil affects the bio-chemical nature of the soil. As a result no vegetation occurs in those areas. Therefore, a study was made with an objective to revive the soil condition for vegetation development. Biochemical nature of soil showed that soil is highly alkaline which is not suitable for seed germination, root and shoot development. Essential elements including heavy metals are detected in the contaminated soil samples. Further, beneficial microbes like nitrogen fixers, phosphate solubilizers, sulphur oxidizers and cellulose degraders and their activities which play a major role for plant growth and soil fertility are recorded less in such contaminated soils. As per the nature of soil sample a bioformulation was developed for reclamation of soil for seed germination and plant growth. Elements recorded in the soil were detected in various parts of the plant

    Iodine a simple, effective and inexpensive catalyst for the synthesis of substituted coumarins

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    Molecular iodine is developed as an e

    Synthesis of 3,4,5,6,7,8-hexahydro-1-substituted phenyl isoquinoline and 3,4,5,6,7,8-hexahydro-1-substituted benzyl isoquinoline

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    Isoquinoline derivatives are obtained from amides using AlCl3-KI in acetonitrile solvent. This cyclo dehydrating Lewis acid produced no side product and yield is above 95% isoquinoline derivative at room temperature

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