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Examunation of Effects of Hylocereus Polyrhizus Britt extract on serum pricnflammations cytokine in aging mice
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The stude of cognition of emergency of occupational hazard in different gender for graduating students
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Inhibitory Analysis of Lactic Acid Bacteria on Vaginal Pathogens
Get PDF論文口試委員會審定書 .................................................................................... i
授權書 ............................................................................................................... ii
致謝 .................................................................................................................. iii
中文摘要 .......................................................................................................... iv
Abstract ............................................................................................................... v
論文目錄 .......................................................................................................... vi
圖表目錄 ........................................................................................................ viii
第一章、緒論 .................................................................................................... 1
1.1.研究背景 ................................................................................................... 1
1.2.研究動機與目的 ....................................................................................... 5
第二章、相關議題探討 ................................................................................. 6
2.1.陰道炎與主要致病菌種 ........................................................................... 6
2.1.1.陰道炎之種類 ..................................................................................... 8
2.1.2.陰道炎主要致病菌種 ....................................................................... 11
2.2.乳酸菌 ..................................................................................................... 14
2.2.1.乳酸菌基本特性 ............................................................................... 16
2.2.2.各乳酸菌屬主要特性簡述 ............................................................... 18
2.2.3.乳酸菌在人體的生理活性 ............................................................... 23
2.2.4.乳酸菌抑菌機制 ............................................................................... 26
第三章、材料與方法 .................................................................................... 33
3.1.材料 ......................................................................................................... 33
3.2.實驗方法 ................................................................................................. 34
3.2.1.篩菌試驗 ........................................................................................... 34
3.2.2.生物膜 ............................................................................................... 35
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3.2.3.酸度 ................................................................................................... 36
3.2.4.產H2O2試驗 ..................................................................................... 36
3.2.5.抑菌試驗 ........................................................................................... 37
3.2.6.最小抑菌濃度(MIC) ......................................................................... 37
3.2.7.實驗結果 ........................................................................................... 38
第四章、討論與結論 ...................................................................................... 42
參考文獻 .......................................................................................................... 44[[abstract]]在近代的社會中，細菌性陰道炎是現代女性最常遇到的健康問題之一，加上台灣氣候潮濕悶熱，私密處困擾應該更加普遍。徹底清潔的觀念只會讓陰道感染更加惡化，即便以抗生素治療，不僅效果不彰且副作用大，覆發率更高達5成。乳酸菌是生殖道中的優勢共生菌種，藉由維持酸性環境、分泌抗菌物質來抑制陰道病原菌種生長或粘附在上皮組織中。本篇論文之目的就是利用乳酸菌所產的乳酸、H2O2、細菌素以及生物膜來對抗陰道病原菌的能力分析。
此實驗篩選出9種乳酸菌菌種共有82株菌。實驗結果顯示乳酸菌的pH值在3.6~4.55之間 (24小時)、3.5~4.5 (48小時)，酸度在0.198~0.045％之間。H2O2和乳酸對 Candida albicans、Gardnerella vaginalis 和Escherichia coli的MIC試驗，H2O2對 Candida albicans 的最小抑菌濃度為0.116mM，對 Gardnerella vaginalis 的最小抑菌濃度為0.029mM，對 Escherichia coli 的最小抑菌濃度為0.0116mM，乳酸對 Candida albicans 的最小抑菌濃度為85％，對 Gardnerella vaginalis 的最小抑菌濃度為4.25％，對 Escherichia coli 的最小抑菌濃度為2.125％。
最後在所有抗菌試驗中發現Lactobacillus reuteri此菌種針對Candida albicans和Gardnerella vaginalis抑制能力效果最佳；Lactobacillus rhamnosus對Escherichia coli的抑制能力最好。
In modern society, modern women bacterial vaginosis is the most commonly encountered health problems , in addition Taiwan climate moist sultry, the feminine private parts problems should be more common. The traditional concept thorough cleaning will only worsen vaginal infections, even with antibiotic treatment, and side effects is not only ineffective, the recurrence rate as high as 50 percent. Lactic acid bacteria are the advantages of the genital tract symbiotic bacteria, by maintaining the acidic environments, the secretion of antimicrobial substances to inhibit the growth of vaginal pathogenic bacteria species or adhesion in epithelial tissues. The purpose of this thesis is the use of lactic acid , H2O2, bacteriocin and the biofilm produced by lactic acid bacteria against the ability of vaginal pathogens.
This experiment screened 9 species of 82 strains of lactic acid bacteria strains. The results showed that lactic acid pH value between 3.6 ~ 4.55 (24h), 3.5 ~ 4.5 (48 h), acidity in the 0.198 ~ 0.045%. H2O2 and lactic acid on Candida albicans, Gardnerella vaginalis and Escherichia coli the minimum inhibitory concentration (MIC) test, H2O2 on the minimum inhibitory concentration of Candida albicans to 0.116mM, on the minimum inhibitory concentration of Gardnerella vaginalis as 0.029mM, of Escherichia coli minimum inhibitory concentration 0.0116mM, lactic acid on Candida albicans of the minimum inhibitory concentration of 85%, of Gardnerella vaginalis in the minimum inhibitory concentration of 4.25%, on the Escherichia coli minimum inhibitory concentration of 2.125%. Finally, all antimicrobial tests found that this strain Lactobacillus reuteri for Candida albicans and Gardnerella vaginalis rejection the best. Lactobacillus rhamnosus against Escherichia coli is the ability to inhibit the best
