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Determination and Comparison of Dissociation and Inhibition Constants of 12α-Hydroxysteroid Dehydrogenase with Inhibitors and Substrates
No full text12α-hydroxysteroid dehydrogenase (12α-HSDH) catalyzes the conversion of cholesterol into bile acids and the interconversion of deoxycholic acid into 12-oxolithocholic acid, making it an important enzyme of the human gut microbiome. Using fluorescence quenching, emission and excitation spectra for the enzyme and variable concentrations of multiple ligands – to include NADP+, NAD+, FAD, deoxycholic acid (DCA), and cholic acid (CA) – were collected and used to create corresponding protein binding saturation curves. Using these curves, the dissociation constants (Kd) for each of the ligands was calculated, with NADP+’s being 3.480 x 10-3 mM, NAD+’s 9.285 x 10-4 mM, FAD’s 3.123 x 10-2 mM, DCA’s 2.008 x 10-5 mM, and CA’s 3.775 x 10-10 mM. As a lower value indicates higher binding affinity, cholic acid appears to have the best affinity for the enzyme, but more research needs to be done to validate the results. Future work will also need to be done to fully compare these values with the inhibition constant (Ki) and Michaelis constant (Km) for each ligand, as they were unable to be ascertained due to time restrictions and technical difficulties
The Social Pot: A Social Media Application
Get PDFThe Social Pot is a web application that allows a user to post to Instagram and X simultaneously from one place. The user creates a Social Pot Account and from there can set their Instagram username and password within the home page. Once the user attempts to post, it will redirect them to login to X which once successful will make the tweet. Used the API \u27instagram-private-api\u27. User needed to give access to my X Project which in turn gave an Auth token (via X redirect URL). The auth token was then sent to my endpoint in order to get an access and refresh token for the user (via X endpoint). The refresh/access token, along with the post data, was then sent to the X endpoint to post the twee
Bridgewater College, Action photograph of tennis players, undated
No full textArchival photograph from Bridgewater College Special Collectionshttps://digitalcommons.bridgewater.edu/tennis_photos/1011/thumbnail.jp
