Biomedical Photonics (BMP - E-Journal)
Not a member yet
274 research outputs found
Sort by
ВЛИЯНИЕ ФОТОАКТИВИРОВАННОГО БЕНГАЛЬСКОГО РОЗОВОГО НА МИКРОЦИРКУЛЯЦИЮ
Get PDFThe study of mean blood velocity changes in rat mesenteric venules (15–30 µm) due to photoactivation (λ = 532 nm, 0,175 W/cm2, 300 s) of i.v. injected Rose Bengal (17 mg/kg) is represented in the article. Using in vivo biomicroscopy the progressive decrease of mean blood velocity both during the laser irradiation and after it was registered. The calculation of thermal effects in the irradiated part of microvessel due to laser absorption by hemoglobin and Rose Bengal showed the rise of temperature was not exceed 0.2оC as compared to initial level. Thus, thermal effect has nearly no significant influence on microvascular disturbances caused by Rose Bengal photoactivation.В работе представлены исследования изменения кровотока в венулах (15–30 мкм) брыжейки крысы при фотоактивации (λ = 532 нм; 0,175 Вт/см2; 300 с) введенного внутривенно бенгальского розового (17 мг/кг). Методом прижизненной биомикроскопии регистрировали прогрессирующее уменьшение скорости кровотока как во время лазерного облучения, так и после прекращения процесса фотоактивации фотосенсибилизатора. Проведённые численные оценки тепловых эффектов в облучаемом участка микрососуда за счёт поглощения лазерного излучения гемоглобином крови и бенгальским розовым показали, что повышение температуры не превышает 0,2оС над исходным уровнем. Таким образом, термический эффект практически не оказывает существенного влияния на расстройство микроциркуляции, вызванное фотоактивацией бенгальского розового
ОЦЕНКА СОСТОЯНИЯ КРОВОТОКА НИЖНИХ КОНЕЧНОСТЕЙ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ МЕТОДОМ ФЛУОРЕСЦЕНТНОЙ АНГИОГРАФИИ В БЛИЖНЕМ ИНФРАКРАСНОМ ДИАПАЗОНЕ
Get PDFThe results of evaluation of the soft tissue perfusion of the foot in patients with diabetes and critical lower limb ischemia (CLI) via fluorescence angiography (FAG) in the near-infrared range are represented in the article. The study included 4 diabetic patients with CLI. All patients underwent lower-limb balloon percutaneous transluminal angioplasty. To evaluate the soft tissue perfusion of the foot via FAG the following parameters have been used: T0m - time to reach maximum for fluorescence intensity after intravenous administration of Indocyanine green; TIm- time of onset of maximum fluorescence intensity after its appearance in the area of interest; Im - the level of the maximum fluorescence intensity. The region of interest was the skin around the wound of the foot. The median FAG parameters in the region of interest on the foot were as follows: prior to surgery T0m = 164 sec (148–181), TIm = 48 sec (38–56); after surgery: T0m = 80 sec (69–92); TIm = 27 sec (20–39); (p <0,05). No adverse reactions were registered in the study. Despite the small sample we were able to achieve statistical significance in the difference between the parameters prior to and after surgery. Further prospective studies with extended samples of patients are needed
ФОТОДИНАМИЧЕСКАЯ ТЕРАПИЯ ПЛОСКОКЛЕТОЧНОГО РАКА КОЖИ ЩЕКИ (КЛИНИЧЕСКОЕ НАБЛЮДЕНИЕ)
Get PDFThe results of the clinical observation of a female patient with squamous cell carcinoma of the cheek skin treated by the method of photodynamic therapy (PDT) using the photosensitizer of fotoditazin are represented. The patient had single course of PDT. Fotoditazin was injected in a dose of 0.8 mg/kg of body weight as a single intravenous infusion in 0.9% sodium chloride. The irradiation was performed once, 2.5 h after injection of the photosensitizer (power density of laser irradiation – 200-300 mW/cm2, light dose of a single session of 300-350 J/ cm2). There were no side effects after photosensitizer injection and after irradiation. The result of the treatment was defined as partial tumor regression. For nowadays the follow-up period is 2 months. The repeated course of PDT is scheduled.Приведены результаты клинического наблюдения за пациенткой с плоскоклеточным раком кожи щеки, пролеченной методом фотодинамической терапии с фотосенсибилизатором фотодитазин. Пациентке проведен однократный курс фотодинамической терапии. Фотодитазин вводили в дозе 0,8 мг/кг массы тела путем однократной внутривенной инфузии в 0,9%-ом растворе натрия хлорида. Облучение проводили однократно, через 2,5 ч после введения фотосенсибилизатора (плотность мощности лазерного излучения – 200-300 мВт/см2, световая доза одного сеанса – 300-350 Дж/см2. Побочных эффектов после введения фотосенсибилизатора и проведения облучения не зарегистрировано. Результат лечения оценен как частичная регрессия опухоли. В настоящее время пациентка находится под динамическим наблюдением, срок наблюдения 2 мес. Планируется проведение повторного курса ФДТ
ФИЗИЧЕСКИЕ МЕТОДЫ РЕАБИЛИТАЦИИ ОНКОЛОГИЧЕСКИХ БОЛЬНЫХ ПОСЛЕ КОМБИНИРОВАННОГО ЛЕЧЕНИЯ РАКА МОЛОЧНОЙ ЖЕЛЕЗЫ
Get PDFAccording to worldwide statistics breast cancer is the most common cancer in women. Despite the success in current methods of treatment post-operative period in major of patients is characterized by post-mastectomy syndrome which leads to impairment in daily activities, difficulties in social adaptation and consequently to dicrease of the quality of life. The specific causes, risk factors, characteristics of clinical and pathogenetic manifestations of post-mastectomy syndrome are described in detail in this review. Different approaches to post-mastectomy syndrome classification, its clinical and instrumental diagnosis are defined. Based on more than 40 publications of Russian and foreign authors, the issue of selection of appropriate treatment tactics for the syndrome is investigated and discussed in detail. A considerable part of the review is focused on the use of physical methods of treating the post-mastectomy syndrome. It is observed that an increasing number of specialists gives preference to methods of conservative treatment special of which are medical gymnastics, complex decongestive therapy and compression therapy. On the other hand, the absence of the unified treatment algorithms is challenging for clinicians. Therefore, its development with the focus on patient-specific approach is a crucial task for modern rehabilitology in breast cancer surgery
МОРФОФУНКЦИОНАЛЬНЫЕ ХАРАКТЕРИСТИКИ САРКОМЫ М-1 КРЫС ПОСЛЕ ФОТОДИНАМИЧЕСКОЙ ТЕРАПИИ С ПРОИЗВОДНЫМ БАКТЕРИОХЛОРОФИЛЛА А
Get PDFThe study of the functional morphology of rat sarcoma M-1 after photodynamic therapy using bacteriopurpurinimide disulfide derivative (disulfide-BPI) as a photosensitizer is described. The research methods included immunohistochemistry for PCNA and CD31, evaluation of mitotic activity and apoptosis of tumor cells, as well as computer analysis of microscopic images. Photoinduced antitumor effect was shown to be due to the destruction of sarcoma M-1 vascular bed, the rapid inhibition of proliferative activity and devitalization of tumor cells by apoptosis and necrosis. It is reasonable to suppose that in the early stages after photodynamic therapy destruction of the microvasculature and photocytostatic shock of tumor cells with subsequent development of necrosis are caused by direct influence of the light flux on sensitized cellular elements in parenchyma and stroma of tumors. The efficiency of photodynamic therapy with the novel photosensitizer is determined by the sequence of destructive and inflammatory changes in tumor parenchyma and surrounding tissues, as well as repopulation potential of tumor cells survived after treatment
Пресс-релиз V Всероссийской конференции с международным участием «Фотодинамическая терапия и фотодиагностика»
Get PDF.
ФОТОСЕНСИБИЛИЗАТОРЫ КАК РАДИОСЕНСИБИЛИЗИРУЮЩИЕ АГЕНТЫ В ЭКСПЕРИМЕНТАЛЬНОЙ И КЛИНИЧЕСКОЙ НЕЙРООНКОЛОГИИ
Get PDFThe article summarizes the main mechanisms underlying the radiosensitizing effect of photosensitizers.According to literature data many authors consider the formation of reactive oxygen species to be the key element in the implementation of the antitumor effect of ionizing radiation, which transfers the photosensitizer molecule from the ground state to the excited state. Their formation leads to the development of oxidative stress. The consequence of the realization of the oxidative stress induced by ionizing radiation is apoptosis of tumor cells.The first photosensitizers, which radiosensitizing activity was confirmed, were hematoprofyrin and photophryn II. A detailed analysis of the results of experimental studies of radiosensitizing effect of photosensitizers on cell culture and laboratory animals with transplanted lines of malignant gliomas (glioma C6, gliosarcoma 9L, glioblastoma U87-MG) was made.The method of tumor radiosensitizing by administration of photosensitizers has been tested in patients with recurrent gliomas grade III-IV in a number of oncological centers. The obtained results show its good tolerability and antitumor efficacy.We have not found publications on the study of the radiosensitizing effect of chlorine-based photosensitizers in the available literature, that makes following studies in this field relevant
ИНТРАОПЕРАЦИОННАЯ ФОТОДИНАМИЧЕСКАЯ ТЕРАПИЯ БОЛЬНОЙ РАКОМ МОЛОЧНОЙ ЖЕЛЕЗЫ IIIC СТАДИИ (8-ЛЕТНИЙ ПЕРИОД БЕЗРЕЦИДИВНОГО НАБЛЮДЕНИЯ)
Get PDFThe article presents a clinical observation of the patient of 38 y.o. with cancer of the left breast stage IIIC урТ4bN3М0L1V1. On the 1st step of the treatment the patient had 2 courses of CAF neoadjuvant chemotherapy, on the 2nd step – extended radical mastectomy on the left with intraoperative photodynamic therapy and closure of the defect with ТRАМ-flap, on the 3rd step – continuation of the chemotherapy (8 courses), on the 4th step – radiation therapy to the chest wall on the left and zones of regional lymph drainage, targeted therapy with herceptin a (1 year). Four years later a silicone implant was inserted into the left breast. Corrective surgery (reduction mammoplasty on the right side) was performed in april, 2017. Currently, the patient has remission of the disease of the left breast, the period of remission accounts for 8 years. В статье представлено клиническое наблюдение пациентки 38 лет с диагнозом рак левой молочной железы IIIС стадии урТ4бN3М0L1V1.На первом этапе лечения пациентке выполнено 2 курса неодъювантной химиотерапии по схеме САF, на втором этапе – радикальная расширенная мастэктомия слева с интраоперационной фотодинамической терапией и закрытием дефекта ТRАМ-лоскутом, на третьем этапе – продолжение полихимиотерапии (8 курсов), на четвертом этапе – лучевая терапия на грудную стенку слева и зоны регионарного лимфооттока, таргетная терапия герцептином (1 год). Через 4 года выполнена установка силиконового эндопротеза в левую молочную железу. Корригирующая операция (редукционная маммопластика справа) проведена в апреле 2017 г. В настоящее время у пациентки ремиссия заболевания левой молочной железы – период ремиссии 8 лет
ФЛУОРЕСЦЕНТНАЯ ДИАГНОСТИКА РАКА МОЧЕВОГО ПУЗЫРЯ С ПРЕПАРАТОМ ГЕКСАСЕНС – РЕЗУЛЬТАТЫ МНОГОЦЕНТРОВОГО КЛИНИЧЕСКОГО ИССЛЕДОВАНИЯ
Get PDFThe results of multicenter trial for efficacy of fluorescence diagnosis for bladder cancer with agent hexasens (SSC Scientific Research Institute Organic Intermediates and Dyes, Russia) based on hexyl ester of 5-aminolevulinic acid compared with routine cystoscopy are represented in the article. The study included 124 patients. All patients had intravesical instillation of 50 ml of 0,2% hexasens. The exposure time accounted for 1-2 h. After removal of the drug from bladder standard cystoscopy followed by fluorescence diagnosis was performed in every patient. During the local fluorescence spectroscopy the intravesical instillation of the agent hexasens was shown to induce selective accumulation of photoactive protoporphyrin IX in tumor cells comparing with intact bladder mucosa. During 1 h after intravesical instillation of hexasens solution the level of fluorescence of hexasens-induced protoporphyrin IX in the tumor was higher than that in surrounding intact mucosa at average in 5.8 fold. As compared with results of standard cystoscopy, fluorescence diagnosis improved the sensitivity of the method by 24.4% (from 75.6% to 100%), the accuracy – by 15.2% (from 83.3% to 98.5%) and negative predictive value – by 33.5% (from 66.5% to 100%). Fluorescence diagnosis gave an opportunity to detect additional tumor foci, which were invisible in white light, in 27.4% of patients. For fluorescence diagnosis in 4.0% of patients false-positive fluorescence of bladder mucosa was detected that was probably due to inflammation. None of patients received active dose of the drug hexasens had adverse reactions, changes in general well-being and blood and urine parameters. According to the results of the study fluorescence diagnosis with hexasens for bladder cancer is recommended for use in clinical practice
ИНТРАОПЕРАЦИОННАЯ ФЛУОРЕСЦЕНТНАЯ ДИАГНОСТИКА ПЕРИТОНЕАЛЬНОЙ ДИССЕМИНАЦИИ У БОЛЬНЫХ РАКОМ ЖЕЛУДКА
Get PDFThe study of diagnostic efficiency of intraoperative fluorescence diagnosis (IOFD) of peritoneal dissemination in patients with gastric cancer comparing with standard laparoscopy was performed in P.A. Hertsen MCRI (the branch of NMRRC). The study included 114 patients with verified diagnosis of gastric cancer stage III–IV. For IOFD the domestic medication alasens (the active agent – 5-aminolevulinic acid) was used orally at dose of 30 mg/kg body weight 3 h prior to the study procedure. As the result of fluorescence diagnosis 10 (6.9%) patients had peritoneal tumor dissemination verified morphologically and undetected by standard laporoscopy. The sensitivity of peritoneal revision for standard laparoscopy in patients with gastric cancer stage III–IV was 60.8%, the specificity – 75.8%, the overall accuracy – 69.1%. The sensitivity for IOFD in patients with gastric cancer stage III–IV was 91.1%, the specificity – 93.9%, the overall accuracy – 92.7%. Thus, fluorescence study allowed improving the specificity, the sensitivity and the overall accuracy of diagnostic test. In the trial IOFD had the maximal diagnostic value in patients with visually undetectable microdissemination in «whight» light and with solitary visible peritoneal foci (the average number of additionally detected metastatic lesions in one patient – 1.22 and 1.4, respectively). Moreover, in the case of fluorescence diagnosis the number of detected microfoci of peritoneal dissemination was showed to increase with enlargement of area of tumor invasion to gastric serosa (at average of 2.29 additionally detected lesions in one patient with serosal invasion more than 2 cm2). The obtained results confirm the perspective of use of fluorescence diagnosis for detection of peritoneal dissemination, determination of true tumor extent, staging of the tumor and also for adjustment of following treatment tactics for this group of patients