Biota: Biologi dan Pendidikan Biologi
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Sintesis dan Optimisasi Produksi Kitosan Asal Cendawan Endofit Akar Cagar Alam ulau Dua Banten
No full textThe cell wall component of root endophytic fungi is a main source of chitosan synthesis. This research aimed to optimize chitosan synthesis from three different isolated species of root endophytic fungi in Pulau Dua Nature Reserve, Banten Indonesia. Three species of root endophytic fungi, namely, Aspergillus niger, Aureobasidium sp., Basipetospora sp., were cultured to produce chitosan. To optimize the production of fungal chitosan, cultures were treated with different conditions such as growth medium (Oat Meal broth, Potato Dextrose Broth, CMMY Broth), pH value (4, 5, 6, 7), and temperature (25, 30, 35, 40°C). Chitosan was extracted from dried mycelium after treated on NaOH 10% at 121°C for 2 min. The degree of deacetylation (DD) of chitosan was then examined and compared with the control (shrimp chitosan). The optimum fungal chitosan condition was higher in CMMY medium, pH 5, and 30°C. The DD value of three root endophytic fungi species, Aspergillus niger, Aureobasidium sp., Basipetospora sp. were 88.5, 83.3, 80.8, respectively, which was lower than DD value resulted from control. This study indicates that Aspergillus niger was a potential endophytic fungi for chitosan synthesis
The Effectiveness of SETS based-learning in Improving Students’ Higher-Order Thinking Skills
No full textThe aim of this study was to investigate a SETS (Science, environment, technology) based-learning approach for junior high school students on how well SETS based-learning improves thinking skill on the waste management. This study was conducted to 20 students of 7th grade. Data collection were employed by class observation (carried out pre and post test), essay analysis (based on several cognitive indicators such as analyze, evaluate, and create), and learning activities regarding environmental issues. The results showed that SETS based-learning could positively improve students critical thinking in terms of learning process and performance in creativity, motivation, critical thinking. If the number of subjects is increase, SETS based-learning strategy could be more challenging
Plankton Biodiversity in The Burai River of Ogan Ilir District, Sumatera Selatan
Full text linkThe purpose of this study was to determine the abundance of plankton in the Ogan Ilir regency of South Sumatra. Samples were obtained using a purposive random sampling method with plankton as the main material for measuring abundance and the Shannon Wiener Diversity Index (H), Simpson Dominance Index (C), and plankton abundance index. The results of the study obtained six classes consisting of 15 genera of plankton including Fragielaria, Synedra, Eunotia, Diatom, Fustulia, Oedogonium, Gonium, Chaetophora, Microspora, Allona, Neuplius, Lyngbya, Spirulina, Cyclotella and Dismidium. Diversity value at point 1 (1.75) with medium category, point 2 (3.5) with high category, point 3 (0.75) with the medium category. The diversity value of three points is known to be low in population
The Growth Comparison of Haematococcus Pluvialis in Two Different Medium
Full text linkMicroalgae is an aquatic microorganism that conducts photosynthesis. It plays a vital role as an oxygen producer in the marine ecosystem. A freshwater microalgae, Haematococcus pluvialis, has been utilized as a health supplement and industrial application which is beneficial for human. In addition to physical and chemical factors, nutrient composition is one crucial thing that contributes to the growth of microalgae. This present study aimed to determine and compare the growth rate of Haematococcus pluvialis cultivated in two culture medium, Walne’s and Guillard. The culture conditions observed were light intensity, photoperiod of light-dark hours, temperature, inoculum concentration of medium’s liquid, and cell density. This study confirmed that Walne’s media produced much higher biomass (247x104 cells/mL) than Guillard’s medium (209.6x104 cells/mL). The aspect to be further performed on H. pluvialis biomass is exploring its high-value bio compound. 
Notes of Some Macroscopic Fungi at IPB University Campus Forest: Diversity and Potency
Full text linkMacroscopic fungi is cosmopolitan-heterotrophic organisms which have an important ecological role in ecocystem. The aim of this study was to explore the diversity of species and the potency of Fungi in the Campus Forest of IPB Unversity. Observation was carried out in March to April 2019 using exploration method. Identification used morphological characteristic such as fruit body shape, hygrophnous, cap color, diameter, edge, and margin, wetness level, himenophore type. Result showed that 11 species of macroscopic fungi were identified, divided into 7 families, 4 order, and 1 class. The Group fungi were identified as Termitomyces sp. 1, Termitomyces sp. 2, Marasmius sp. 1, Marasmius sp. 2, Psathyrella sp., Geastrum sp., Stereum sp., Microporus sp., Polyporus sp., Ganoderma sp. 1 and Ganoderma sp. 2. All identified fungi were Basidiomycota. Some fungi found to be potentially used as food source, medicine, and also played an important role as a decomposer in the IPB university campus forest
Piebaldism in To Balo, South Sulawesi: Brief Report and Literature Review
Full text linkPiebaldism is an autosomal dominant congenital disorder in pigment as a result of mutations in KIT gene, mapped on the long arm of chromosome 4. To Balo lived as a carrier of piebaldism genes. There are only four individuals of To Balo communities remained on Pujananting district, South Sulawesi. This study was a brief report and literature review about suspects of Piebaldism in To Balo. Data was collected by observation of depigmentation, interview and then depicted in pedigree. Phenotypic observations resulted in piebaldism on three individuals of To Balo, showed the same pattern and location of white patches. It is concluded that normal individuals could appear in third-generation when a woman or man carrier of piebaldism married with a normal individual
Public Service Motivation: The Teaching Performance of Biology Teachers
Full text linkThe objective of this research was to explore the impact of Public Service Motivation (PSM) on the teaching performance of biology teachers at Madrasah Aliyah in Central Lombok Regency. The research method employed was a quantitative approach. This research was conducted on 32 samples of biology teachers at Madrasah Aliyah (High School) in Central Lombok Regency. Data were collected through a structured questionnaire containing statements filled out by respondents using a Likert Scale and scores of the respondent’s answer in each variable. Statistical calculations showed that the correlation value (r) between the Public Service Motivation (PSM) and the Teachers Performance variables was positive (0.278). However, the correlation between the Public Service Motivation and the Teachers Performance variables occurred was not significant. This present study suggests that Public Service Motivation has a positive correlation with the Teaching Performance
Diversity of Butterflies (Lepidoptera: Rhopalocera) in Sumber Clangap and Waduk Selorejo, East Java
Full text linkDiversity of butterflies in various habitats very diverse, and it is one of them in the region Sumber Clangapand Waduk Selorejo. It has seen from the combination of colors and which wing shape varies. In this, bothregions were chosen because it had been affected by Mount Kelud eruption, which causes damage to thesurrounding area. The purpose of this research is to know the diversity of butterflies (Lepidoptera:Rhopalocera) in the region of Sumber Clangap and Waduk Selorejo, East Java. This research wasconducted on 26-29 January 2019. Sampling has taken by the method of Belt transect along the transectpath randomly with a technique snapshot experiment and a sweeping technique. Based on theobservations obtained 3 of the same family, Nymphalidae, Pieridae, and Papillionidae. The butterflydiversity in Waduk Selorejo belongs to low, whereas in Sumber Clangap are classified by index Shannon-Winner = 0.38 H ' and H ' = 2.47. Found eight types of butterflies from 3 families with a total of 13 speciesand has a value of H ' =2.47
ISOLATION OF CHITOSAN SNAIL SHELL (Achatina fulica F) ON CHEMICAL CHARACTERISTICS AND BACTERIA OF Staphylococcus aureus KIRBY BAUER IN VITRO METHOD
No full textThe shell of snail contains chitin that can be transformed into chitosan through four stages, which are deproteination, demineralization, depigmentation, and deacetylation. Chitosan can be utilized as an antibacterial agent because it has amine groups. In this research, chitosan that prepared from the snail shell will be analyzed using FT-IR spectroscopy to determine the deacetylation degree (DD). The antibacterial activity test result of Staphylococcus aureus will be carried out on chitosan with various concentrations using in-vitro Kirby Bauer method and will be analyzed using One Way ANOVA with confidence level at 95%. The variations concentration of chitosan used is 300, 400, 500, 600, and 700 ppm. The result shows that the deacetylation degree of chitosan is 63.09% and the optimum concentration of chitosan that can inhibit the Staphylococcus aureus bacteria is 500 ppm with diameter inhibition is 34.33 mm.Cangkang bekicot mengandung kitin yang dapat diisolasi menjadi kitosan. Kitosan diketahui memiliki sifat antibakteri kerena memiliki gugus amina. Tujuan penelitian ini untuk mengetahui isolasi kitosan cangkang bekicot (Achatina fulica F) terhadap karakteristik kimia dan bakteri Staphylococcus aureus dengan metode kirby bauer secara In Vitro dan berapakah konsentrasi kitosan cangkang bekicot yang paling optimal dalam menghambat bakteri Staphylococcus aureus. Penelitian ini menggunakan jenis penelitian true experimen. Analisa data dengan uji One-Way ANOVA dengan taraf kepercayaan 95% menunjukkan terdapat perbedaan yang bermakna (p<0,05) antara berbagai konsentrasi, sehingga dilanjutkan dengan uji LSD untuk mengetahui adanya perbedaan pada masing-masing perlakuan. Hasil menunjukan bahwa derajat deasetilasi kitosan sebesar 64,24 %. Hasil uji LSD menunjukkan bahwa kosentrasi 300 ppm dan 400 ppm berbeda nyata dengan konsentrasi 500 ppm, 600 ppm, dan 700 ppm, sedangkan konsentrasi 500 ppm dan 600 ppm tidak berbeda nyata dengan konsentrasi 700 ppm. Kesimpulan penelitian ini bahwa ekstrak kitosan cangkang bekicot berpengaruh positif dalam menghambat dan membunuh bakteri Staphylococcus aureus dan konsentrasi paling optimal pada 500 ppm. Kata kunci : Cangkang bekicot (Achatina fullica), kitosan, Metode Kirby Bauer, Staphylococcus aureu
Pengaruh Ekstrak Daun Mimba (Azadirachta indica A. Juss) Terhadap Mortalitas Hama Lalat Buah Cabai (Bactrocera dorsalis L.)
Full text linkLalat buah cabai merupakan hama utama pada tanaman cabai. Lalat buah cabai dapat dikendalikan dengan berbagai jenis insektisida, salah satunya adalah insektisida nabati yang salah satunya dengan memanfaatkan daun mimba. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun mimba untuk mengendalikan hama lalat buah cabai pada stadium larva, pupa dan imago. Jenis penelitian ini adalah eksperimen dengan pendekatan kuantitatif. Populasi pada penelitian ini adalah semua larva, pupa dan imago hama lalat buah cabai yang dibiakkan. Penelitian ini menggunakan lima perlakuan dan lima ulangan dengan sampel penelitian 10 ekor untuk tiap ulangan. Adapun kesimpulan dari penelitian ini adalah ekstrak daun mimba tidak memliki pengaruh yang nyata untuk mengendalikan hama pupa lalat buah cabai sedangkan pada larva dan imago ekstrak daun mimba memliki pengaruh yang nyata untuk mengendalikan hama tersebut