Biota: Biologi dan Pendidikan Biologi
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Identification of Edible Macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia
Full text linkIndonesia is considered as a mega-biodiversity country that has a massive amount of vascular and non-vascular plants. The tropical environment condition of Indonesia could support the growth of macrofungi. Information about edible macrofungi from the Forest of Lombok Island is based on limited data. This research aims to characterize the edible macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia. This research was a descriptive and explorative study. The edible mushrooms were observed through the Cruise method by following the particular track inside the forest. The sample found in the forest then documented and evaluated. A morphological analysis procedure was performed to assess the profile and similarity between the microscopic evaluations with the mushrooms identification book. In this study, we also offered a phylogeny analysis based on morphological characters similarity. The Dendogram tree was reconstructed using PAST 3.0. software. The result showed that there are eight species of edible mushrooms found that were group into Basidiomycota, namely, Termitomyces clypeatus, Termitomyces umkowaan, Termitomyces sp.1, Pleorotus flabelatus, Pleurotus ostreatus, Coprinus desimenatus, Tremella fuciformis, and Polyporus sp. All of them involved in Basidiomycota. Based on characterization and identification, the species are grouped into three Ordos and five Families. Most of the edible macrofungi found at Kerandangan Protected Forest & Natural Park growth at soil and deadwood.
 
Rheotaxis Response Based on Sexual Dimorphism in the Green Swordtail Fish, Xiphophorus hellerii
Full text linkMorphological distinctions between males and females of a species are referred to by sexual dimorphism. It may result from various selection pressures affecting either sex or both and may occur in any dioecious species, including Green Swordtail fish, which are sexually reproductive. This study examined the different rheotaxis responses of Xiphophorus hellerii based on different sexes and morphological features. We analyzed ten adult males, ten gravid females, and ten non-gravid females of Xiphophorus helleri collected down the river and transferred into the column. We counted the number of the individual that performed positive rheotaxis (+), negative rheotaxis (-), and indifference response (0). The result showed different rheotaxis responses shown by male, non-gravid female, and gravid female X. hellerii. The highest percentage of positive rheotaxis response (movement against the current) was shown by non-gravid female X. hellerii, reaching up to 89%. Morphological differences between male, non-gravid female, and gravid female X. hellerii appear to affect the orientation and ability of X. hellerii in giving response against current and certainly has an impact on their survival in nature
Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure
Full text linkMethoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC
In-silico Approach for The Prediction of Chlorogenic Acid as PPAR-γ Activator
Full text linkCoffee is one of the essential crops commonly cultivated in Indonesia. Coffee contains diverse bioactive compounds, which are associated with human health benefits. One of the compounds is Chlorogenic acid, which able to decrease the risk of type 2 diabetes mellitus (T2DM). However, the mechanism of chlorogenic acid toward anti-diabetes still unclear. This study aimed to analyze and investigate the potential role of chlorogenic acid as anti-diabetes through their interaction with Peroxisome proliferator-activated receptor gamma (PPAR-γ) as an enzyme to phosphorylate and regulate the mechanism of T2DM. The physicochemical properties of chlorogenic acid also performed in this study. The PPAR-γ was downloaded from the PDB database, and the chlorogenic acid was retrieved from the PubChem database. The protein and ligand were prepared using the PyRx program and were docked using Hex 8.0.0 software. Discovery Studio client 4.1 software was used to analyze the interaction between chlorogenic acid and PPAR-γ protein. Based on the physicochemical properties, chlorogenic acid is highly permeable to the cell and easily absorbed. Thirteen amino acid residues of PPAR-γ (GLN410, SER394, ASP396, GLY395, GLU407, LEUA401, LEU400, VAL403, LYS373, LYS438, LEU377, LYS434, and GLY437) were identified on the chlorogenic acid-PPAR-γ interaction. Interestingly, the kind of interactions, including hydrophobic interaction, hydrogen bond, and van der Waals, which are supported by the tight interaction. Our study indicated that chlorogenic acid might have anti-diabetes activity through PPAR-γ interaction.
 
Biolarvacide of Culex quinquefasciatus
Full text linkBiolarvicide is the larvicide derived from plants and relatively safe. The objective of this study was to determine the effect of pletekan leaf powder (Ruellia tuberosa), mimosa (Mimosa pudica), papaya (Carica papaya), and guava (Psidium guajava) on Culex quiquefasciatus larval mortality. The method was an experimental with a completely randomized design consisted of 5 treatments (0 gr, 0,25 gr, 0,5 gr, 0,75 gr, and 1 gr) and 5 repetitions for each plant. The data were analyzed by analysis of variance. Based on the results, leaf powder used in the study, namely guava leaves powder (1 gr), mimosa (1 gr), leaves of pletekan (1 gr), and leaves of papaya (1 gr) showed a very significant effect (P < 0,05) on Culex quiquefasciatus larval mortality. It can be concluded that the pletekan, mimosa, papaya, and guava had the potential as biolarvicide of Culex quinquefasciatus.
 
English
No full textObesity caused by a high-fat diet leads to an altered reproductive hormonal profile, including impaired semen quality. Antioxidants can overcome these conditions. One of the well-known sources of antioxidants is in the rambutan fruit peel extract. This research aimed to figure the effect of rambutan fruit peel extract towards total sperm count in Wistar rats induced with high-fat feed. The study design used post-test only control group, subjects were 30 male Wistar rats divided into five groups: Group I (Positive Control) was given high-fat feed, Group II (Treatment Control Group) was given 15mg/kg BW rambutan fruit peel extract, whereas group III, IV, V (Treatment Group) were given an extract of rambutan skin with a dosage of 15, 30, and 60 mg/kg BW, respectively. Treatment was administered for 81 days. This study showed that rambutan fruit peel extract with doses of 15, 30, and 60 mg/kg BW has significantly increased total sperm count in Wistar rats induced with high-fat feed. The outcome using Kruskal Wallis shows a result of p-value 0,010 (CI 95%). Antioxidant compounds found in rambutan fruit peel extract significantly increase total sperm count in Wistar rats induced with high-fat feed, with the highest efficacy on the dose of 15mg/kg BW.
 
Foot Print of Macro Fungi in The Coastal Forest of Bama, Baluran National Park, East Java
Full text linkBaluran National Park, West Java, as one of the conservation sites in Indonesia, has the attraction of the varied types of ecosystems, including fungi. This study aimed to analyze the diversity of fungi in Bama Coastal Forest, Baluran National Park. The method was explorative with plot purposive sampling technique. Parameters in this study include abundance, dominance, and diversity of fungi enriched with physical parameters of humidity and temperature. The fungi were documented and macroscopically observed. Data were analyzed using the abundance index, dominance index, and diversity index. This research identified 18 types of macrofungi in Bama Coastal forest, Baluran National Park East Java including Ganoderma, sp, Hexagonia tenuis, Trametes hirsute, Phellinus sp.1 and sp.2, Ganoderma applanatum, Phellinus igniarius, Pycnoporus cinnabarinus, Daedalea quercina, Tyromyces chioneus, Microporus xanthopus, Calvatia sp., Irpex lacteus, Trichaptum sp., Lentinus sp. Poria corticola, Tyromyces sp., and Lichemomphalia sp. One fungi species (Ganoderma sp.) has the highest abundance index (27.62). The Medium abundance index was found in three species (Hexagonia tenuis, Tyromyces chioneus, Tyromyces sp.) while the rest fungi were in low abundance index. Dominance index of all fungi species identified in Bama was in low category (D<0.5) with medium diversity (2.30). It can be concluded that the diversity of microfungi in the coastal forest of Bama, Baluran National park was a distribution medium of individual of each species and medium community stability. Environmental conditions in the Bama Coast Forest with 93% humidity and an average air temperature of 26-27oC could support for the fungi to grow quite well
Stomata Profile Comparisons in Abaxial and Adaxial Zones of Dendrobium aphyllum and Arachnis flos-aeris Leaves
Full text linkStomata activity in orchids is related to orchids' adaptation process to their environment. This study aimed to investigate the stomata profile, comprising of anatomical structure and density of the two orchids species, Dendrobium aphyllum, and Arachnis flos-aeris. Data was collected by printing the leaves' surface using glue and observed at 09.00 am and 09.00 pm, then analyzed using Image raster 3.0 software and MS. Excel. The stomata of D. aphyllum and A. flos-aeris were characterized by anomocytic type. Results showed that the highest stomata density was 1177.78 um2, found on the abaxial part of D. aphyllum. The adaxial leaves of both species were classified as low or no stomata. These two orchid species were epiphyte yet grow in different habitats. Arachnis flos-aeris exposed to direct sunlight tends to reduce transpiration rate, resulting in fewer stomata than D. aphyllum less exposed to sunlight
Karakteristik Habitat dan Konservasi Amfibi di Pulau Lombok
No full textThe previous study stated that there are 12 species of Amphibians found in Lombok Island, Indonesia. Four of which are the endemic species of Lesser Sunda Islands, namely Oreophryne monticola, Limnonectes kadarsani, Limnonectes dammermani dan Hylarana florensis. However, the information regarding the habitat characteristic which supports animal survival is limited. This study aimed to explore the types of habitat characteristics according to amphibian diversity in the Island of Lombok. The survey study was conducted at 9 locations (3 of habitat type) from March to July 2016. The collected data includes species composition and a sum of individuals of species. Amphibian diversity was analyzed using relative abundance and the Shannon-Wiener index. This study revealed that the nonforest habitat type was appropriate for five species, while secondary and primary forest habitat type was appropriate for seven species. Diversity of species index directly proportional to a natural level of habitat (H’ Nonforest = 1.3; H’ Secondary Forest = 1.7; H’ Primary Forest = 1.8). The highest species abundance was Bufo melanostictus (23.9%), and the lowest species abundance was Oreophryne monticola (0.9%). According to the distribution and relative abundance, the species that require conservation priorities are Oreophryne monticola and Hylarana florensis
Floristic Composition, Distribution, and Association of Pandanus (Pandanaceae) in Bukit Duabelas National Park Jambi
Full text linkPandanus species is widely distributed over Bukit Duabelas National Park in Jambi. Suku Anak Dalam (SAD), a local community living in Bukit Duabelas National Park area, use the natural fibers from Pandanus for many necessities, such as plaiting, building materials, and performing mytological rituals. This study aimed to examine floristic composition, distribution patterns, and association of pandanus species in Bukit Duabelas National Park. Sampling was conducted using a striped line, with a plot of 35 sample plots. Dominance of species was calculated using dominant type of important Value Index (IVI) based on the density, frequency, and dominance of sample plot enumeration. Distribution patterns was analyzed using the Morisita Index, while association patterns were measured by Contingency tables and Chi-square tests. Floristic composition was mostly found in the seedling strata consisting of 106 species. The species with highest important value index (IVI=14.53) was Palaquium gutta. There were two species of Pandanus (Benstonea kurzii and Benstonea atrocarpa) were spread in groups and three species of pandanus (Pandanus labyrinthicus, Pandanus furcatus, and Pandanus immersus) were spread evenly. The pandanus species found was associated with Benstonea kurzii was Litsea sp1. This present study suggested that the conservation of pandanus species should be conducted as a piece of rehabilitation of tropical forests in Bukit Duabelas National Park