e-Journal Badan Penelitian dan Pengembangan Kesehatan / National Institute of Health Research and Development
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Kelangsungan Hidup Bayi dengan Berat Lahir Rendah (BBLR) Neonatal Berdasarkan Aspek Pelayanan Kesehatan
No full textLow birth weight (LBW) babies means infants that are succeptible to the diseases. Thus, their survival is low. Several previous studies have suggested that survival is related to the management of infants in the health care. Therefore, to measure LBW survival in Indonesia, this study was conducted. Sources of data in this study were Indonesia Demographic and Health Survey (SDKI) in 2002-2003 and 2007. This study used retrospective cohort as its design study. Based on inclusion criteria (singleton birth and birth weight between 1,500 and 2,499 grams) and exclusion (not last child and incomplete data), a number of 1,123 infants was selected. Survival was measured using cox proportional hazard analysis. The results of this study described that LBW survival was 97.33%. In addition, LBW survival rates in mothers using health services were lower than those of LBW with mothers who did not use them. Therefore, the quality of health services should be improved in order to improve the survival of baby LBW. Abstrak Bayi dengan berat lahir rendah (BBLR) merupakan bayi yang rentan terhadap penyakit. Dengan demikian, ketahanan hidupnya rendah. Beberapa studi mengatakan bahwa ketahanan hidup tersebut berhubungan dengan penatalaksanaan bayi di pelayanan kesehatan. Oleh karena itu, untuk mengukur ketahanan hidup BBLR di Indonesia tersebut, penelitian ini dilakukan. Sumber data dalam penelitan ini adalah Survei Demografi dan Kesehatan Indonesia (SDKI) pada tahun 2002-2003 dan 2007. Desain penelitian yang digunakan adalah studi kohort retrospektif. Berdasakan kriteria inklusi (kelahiran tunggal dan berat lahir 1.500 – 2.499 gram) serta eksklusi (bukan anak terakhir dan data tidak lengkap), diperoleh sampel sebesar 1.123 bayi. Ketahanan hidup diukur dengan menggunakan analisis survival dan cox proportional hazard. Hasil studi ini mengatakan ketahanan hidup BBLR sebesar 97,33%. Selain itu, angka ketahanan hidup BBLR pada ibu yang memanfaatkan pelayanan kesehatan lebih rendah dibandingkan dengan BBLR dengan ibu yang tidak memanfaatkannya. Oleh karena itu, kualitas pelayanan kesehatan harus ditingkatkan agar mampu meningkatkan ketahanan hidup bayi BBLR
Ketersediaan Sumber Daya Manusia Kesehatan pada Fasilitas Kesehatan Tingkat Pertama dalam Era Jaminan Kesehatan Nasional di Delapan Kabupaten-Kota di Indonesia
No full textImplementation of the National Health Insurance program causes various effects, including an increased number of visits to primary health facilities, so it takes an adequate distribution of human resources.The aims of this study is to describe the availability of human resources for health in primary health centers in the era of National Health Insurance. Type of research is quantitative-qualitative method withcross sectional approach. Data collecting has done by interviews and round table discussion. Researchlocations were selected purposively in eight districts/cities, namely Bekasi City and Bogor District (WestJava), South Tangerang City and Serang District (Banten), Yogyakarta City and Bantul District (DIY),Surakarta City and Sragen District (Central Java). Informants are leaders/representatives of primaryhealth centers, clinics, physicians and the district/city health office. The quantitative data were analyzed descriptively and qualitative data using content analysis. Health centers in eight districts/cities do notall have the human resources for health in accordance of Permenkes RI No. 75/2014, but general practitioners, midwives and nurses have been available in all health centers though the amount isstill lacking. With the exception of Bogor, the number of medical personnel throughout the clinic is inconformity with Permenkes RI No. 9/2014, but other types of human resources for health is still a lot that has not been available. Meanwhile, throughout the medical practitioners, the most human resources widely available are general practitioners and nurses. There are changes in procurement planning ofhuman resources in the era of National Health Insurance, increased workload and working hours, sothat it is needed planning and procurement of human resources based on needs.Keywords: human resources, primary health facilities, national health insuranceAbstrakPelaksanaan program Jaminan Kesehatan Nasional (JKN) menimbulkan berbagai dampak, termasuk meningkatnya jumlah kunjungan ke Fasilitas Kesehatan Tingkat Pertama (FKTP), sehingga dibutuhkan sumber daya manusia (SDM) kesehatan yang memadai. Tujuan penelitian adalah untuk memberi gambaran tentang ketersediaan SDM kesehatan di FKTP dalam era JKN. Jenis penelitian adalah kuantitatif-kualitatif dengan pendekatan cross sectional. Pengumpulan data dengan wawancara dan round table discussion. Lokasi penelitian dipilih secara purposive di 8 kabupaten/kota yaitu Kota Bekasi dan Kabupaten Bogor (Jawa Barat), Kota Tangerang Selatan dan Kabupaten Serang (Banten), KotaYogyakarta dan Kabupaten Bantul (DIY), serta Kota Surakarta dan Kabupaten Sragen (Jawa Tengah). Informan yaitu pimpinan/wakil institusi puskesmas, klinik, dan praktik dokter serta dinas kesehatan kabupaten/kota. Analisa data kuantitatif dilakukan secara deskriptif dan analisa data kualitatif dengan content analysis. Puskesmas di 8 kabupaten/kota belum seluruhnya memiliki SDM kesehatan sesuaiPermenkes RI Nomor 75 tahun 2014, namun dokter umum, bidan dan perawat telah tersedia di seluruh puskesmas meskipun dengan jumlah yang masih kurang. Kecuali Kabupaten Bogor, jumlah tenaga medis di seluruh klinik sudah sesuai Permenkes RI Nomor 9 Tahun 2014, namun jenis SDM kesehatan lain masih banyak yang belum tersedia. Sedangkan di seluruh praktik dokter, SDM kesehatan yangpaling banyak tersedia yaitu dokter umum dan perawat. Terdapat perubahan dalam perencanaan pengadaan SDM di daerah sesudah JKN, peningkatan beban kerja dan jam kerja, sehingga diperlukan perencanaan dan pengadaan SDM berbasis kebutuhan.Kata Kunci: SDM, FKTP, JK
Risiko Penularan Demam Berdarah Dengue berdasarkan Maya Indeks dan Indeks Entomologi di Kota Tangerang Selatan, Banten
No full textSouth Tangerang City become the highest contributor of dengue in 2014 in the province of Banten. The increasing of dengue cases in this city indicates that transmission still ongoing and the vector controlsless optimal. The aim of this study is to assess the transmission risk of dengue in endemic regions based Maya index and Entomology index. This cross sectional study was conducted in three health centers which highest dengue case at last three years that is Benda Baru, Bakti Jaya and Pondok Jagung in June2015. The survey larvae in containers has conducted in 100 houses in each area of the health center,so that the total sample taken is 300 houses. Containers were observed categorized into containers of controlled / Controllable Containers (CC) and containers used / Disposable Container (DC). Datawere analyzed descriptively to determine the proportion of the number and types of containers. Mayaindex obtained from categorization ratio of Breeding Risk Indicator (BRI) and Hygiene Risk Indicator(HRI). Container Index (CI), House Index (HI), Bruteau Index (BI), House Pupa Index (HPI), Pupa Index (PI) were calculated to assess the density of larvae. The results showed 833 containers with 785 containers belonging to CC and 48 included in the DC. The largest of positive Controllable Container inSouth Tangerang City is a bucket (22.7%), bath up (15.5%) and water reservoirs in dispenser (12.4%),while Disposable Container most positive larvae are buckets former (10.3%) and used goods (7.2%).Value Container Index (CI) was 11.7%, House Index (HI) 27.3%, Angka Bebas Jentik (ABJ) 72.7%,and Bruteau Index (BI) 32.3%. The number of pupae depicted with PI 29.3% and HPI 2.7% which is relatively low. Based on Maya index and Entomology index South Tangerang city has a moderate risk level in the transmission of dengue.Keywords: DBD, Entomology Index, Maya index, South Tangerang City AbstrakKota Tangerang Selatan menjadi penyumbang tertinggi DBD tahun 2014 di Provinsi Banten. Peningkatan kasus DBD tiap tahun di wilayah ini menunjukkan penularan masih berlangsung dan upaya pengendalian yang dilakukan kurang optimal. Oleh sebab itu dilakukan penelitian untuk menilai risiko penularan DBD di wilayah endemis DBD berdasarkan Maya Indeks dan indeks entomologi. Penelitian dilakukan dengan pendekatan potong lintang di tiga puskesmas endemis tertinggi tiga tahun terakhir yaitu Benda Baru,Bakti Jaya dan Pondok Jagung Kota Tangerang Selatan bulan Juni 2015. Survei jentik pada kontainerdi 100 rumah di masing-masing wilayah puskesmas sehingga total sampel yang diambil adalah 300rumah. Kontainer yang diamati dikategorikan menjadi kontainer terkendali/Controllable Containers (CC)dan kontainer bekas/Disposable Container (DC). Data dianalisa secara deskriptif untuk menentukan proporsi jumlah dan jenis kontainer. Maya Indeks diperoleh dari hasil pengkategorian rasio Breeding Risk Indicator (BRI) dan Hygiene Risk Indicator (HRI), Container Index (CI), House Index (HI), BruteauIndex (BI), House Pupa Index (HPI), Pupa Index (PI) dihitung untuk menilai kepadatan larva. Dari hasil pengamatan diperoleh 833 kontainer dengan 785 kontainer tergolong CC dan 48 termasuk dalam DC.Controllable Container yang positif larva terbanyak di Kota Tangerang Selatan adalah ember (22,7%),bak (15,5%) dan penampungan air di dispenser (12,4%), sedang Disposible Container yang paling banyak positif larva adalah ember bekas (10,3%) kemudian barang bekas (7,2%). Nilai Container Index(CI) sebesar 11,7% dan House Index (HI) 27,3%, Angka Bebas Jentik (ABJ) 72,7%, dan Bruteau Index(BI) 32,3%. Indeks pupa digambarkan dengan PI 29,3% dan HPI 2,7% yang masih relatif rendah.Wilayah endemis DBD Kota Tangerang Selatan memiliki tingkat risiko sedang dalam penularan DBD.Kata Kunci: DBD, indeks entomologi, Maya Indeks, Tangerang Selata
Pengaruh Penyalutan Terhadap Karakteristik Fisika Kimia dan Stabilitas Tablet Fraksi Etil Asetat Daun Gambir sebagai Agen Antidislipidemia
No full textGambir (Uncaria gambir, Roxb) with the main content of catechin compounds is a major comodity that is efficacious as an antidyslipidemia agent and reduce lesion atheloschlerosis. Catechins are found most often in gambir leaf extract, but are hygroscopic in order to increase the content and its stability is made in the fraction form and formulated in the film-coated tablet preparation. Coatings will protect the gambir from environmental influences. This study aims to find out the best formula of core tablets and film-coated tablets of ethyl acetate fraction of gambir leaf extract. The granulation method used using wet granulation. Optimization of the tablets coating formulation was carried out through three different formulas with the addition of 4, 6, and 8% coatings. The core tablet and the coat were tested for physical characteristics such as weight uniformity, crushed time, hardness, and tablet fragility, chemical characteristics of active substance content and accelerated stability test. The chemical physics evaluation of tablets shows all tablets meeting physical and chemical requirements. Accelerated stability test result obtained catechin content in the core tablets and coat membrane in the absence of loss potency more than 5%. The coated tablet has loss in potency smaller than core tablet. The greater the composition of the coating material used of coating the smaller the loss in potency of catechin content in tablets.AbstrakTanaman gambir (Uncaria gambir, Roxb) dengan kandungan utama senyawa katekin merupakan komoditas unggulan yang berkhasiat sebagai agen antidislipidemia dan mengurangi lesi aterosklerosis. Katekin ditemukan paling banyak pada ekstrak daun gambir, namun bersifat higroskopis sehingga untuk meningkatkan rendemen kandungan dan kestabilannya dibuat dalam bentuk fraksi dan diformulasikan dalam sediaan tablet salut selaput. Penyalut akan melindungi gambir dari pengaruh lingkungan. Penelitian ini bertujuan untuk mengetahui formula terbaik tablet inti dan tablet salut selaput fraksi etil asetat ekstrak daun gambir. Metode granulasi yang digunakan menggunakan granulasi basah. Optimasi formulasi penyalutan tablet dilakukan melalui tiga formula berbeda dengan penambahan bobot bahan penyalut 4, 6, dan 8%. Tablet inti dan salut dilakukan pengujian karakteristik fisika seperti keseragaman bobot, waktu hancur, kekerasan, kerapuhan tablet, karakteristik kimia berupa kadar zat aktif, dan uji stabilitas dipercepat. Evaluasi fisika kimia tablet menunjukkan semua tablet memenuhi persyaratan fisik dan kimia. Hasil uji stabilitas dipercepat diperoleh kandungan katekin dalam tablet inti maupun salut selaput tidak adanya loss in potency yang lebih dari 5%. Tablet salut memiliki loss in potency lebih kecil daripada tablet inti. Semakin besar komposisi bahan penyalut yang digunakan semakin kecil loss in potency kandungan katekin dalam tablet
Perbandingan Abbott Real-time High-risk HPV dan Cobas 4800 HPV Test untuk Deteksi Molekuler HPV pada Sampel Indonesia
No full textBased on the results of 2013 National Basic Health Research (Riskesdas 2013), as many as 10.3% of female death due to cancer in Indonesia caused by cervical cancer of Human Papillomavirus (HPV) became the main risk factor that was 99.7%. The incidence of cervical cancer can be prevented through early detection of HPV infection moleculary. This study aims to evaluate the ability of Abbot Real-time High-risk HPV (AR) and Roche Cobas HPV (CB) techniques to detect HPV DNA from Indonesia‘s Stored Biology. Furthermore, the results from both techniques are confirmed by using genotyping method using Linnear Array (LA). A total of 74 speciment of stored biological materials (BBT) of cervical swabs stored in Phosphate Buffered Saline (PBS) media were extracted using a special isolation kit from Abbott Real-time High-risk HPV, Cobas 4800 Human Papillomavirus Test and Linear Array. Concentration and purity of HPV viral DNA extraction results were measured by spectophotometry method. The amplification of the extraction results was carried by using a specific primer segment of the L1 gene as a target for detecting HPV DNA. The quality of HPV DNA extraction results using AR technique was 10-30 ng/μL concentration in average, the result was higher when compared to the CB technique which only range from 10-20 ng/μL, but the results using the LA technique had a concentration ≥ 30 ng/μL in average. The purity of HPV DNA extraction using AR and CB technique was similar more or less. The examination of 74 samples had found HPV 16, HPV 18, and other HPV type in each technique, which were 14 positive (9, 4, and 1) in AR technique, 5 positive (3,2, and 0) in CB technique, and 14 positive (8, 4, and 2) in LA technique. AR technique is more accurate and optimal than CB technique in detecting HPV virus and identifying HPV virus types. Both AR and CB technique have their own advantages and disadvantages, although they have uses an automated system of isolation and readout results analysis. It could be concluded that AR technique has better performance in detecting HPV when compared to CB technique. Abstrak Berdasarkan hasil Riset Kesehatan Dasar (Riskesdas) 2013, sebanyak 10,3% kematian wanita akibat kanker di Indonesia disebabkan oleh kanker serviks dengan infeksi Human Papillomavirus (HPV) menjadi faktor risiko terbesar yakni 99,7%. Kejadian kanker serviks dapat dicegah melalui deteksi awal infeksi virus HPV secara molekuler. Penelitian ini bertujuan untuk mengevaluasi kemampuan teknik Abbot Real-time High-risk HPV (AR) dan Roche Cobas HPV (CB) dalam mendeteksi DNA virus HPV dari Bahan Biologi Tersimpan (BBT) Indonesia. Selanjutnya hasil dari kedua teknik tersebut dikonfirmasi dengan menggunakan metode genotyping menggunakan Linear Array (LA). Sebanyak 74 spesimen BBT usap serviks yang disimpan dalam media Phosphate Buffered Saline (PBS) diekstraksi menggunakan kit isolasi khusus dari Abbott Real-time High-risk HPV, Cobas 4800 Human Papillomavirus Test, dan Linear Array. Konsentrasi dan kemurnian hasil ekstraksi DNA virus HPV diukur dengan metode spektofotometri. Amplifikasi hasil ekstraksi dilakukan dengan menggunakan primer spesifik segmen gen L1 sebagai target untuk mendeteksi DNA virus HPV. Kualitas hasil ekstraksi DNA virus HPV menggunakan teknik AR rata-rata mencapai konsentrasi 10-30 ng/μL, hasil tersebut lebih tinggi bila dibandingkan dengan teknik CB yang hanya berkisar pada 10-20 ng/μL namun hasil menggunakan teknik LA rata-rata memiliki konsentrasi ≥ 30 ng/μL. Kemurnian hasil ekstraksi DNA virus HPV menggunakan teknik AR dan teknik CB kurang lebih sama. Hasil pemeriksaan dari 74 sampel ditemukan tipe HPV 16, HPV 18, dan HPV lainnya pada masing-masing teknik, yaitu teknik AR 14 positif (9; 4; dan 1), teknik CB 5 positif (3;2; dan 0), dan teknik LA 14 postif (8;4; dan 2). Teknik AR lebih akurat dan optimum dibandingkan dengan teknik CB dalam mendeteksi virus HPV dan mengidentifikasi tipe virus HPV. Tiap teknik AR dan CB memiliki keunggulan dan kekurangan sendiri, walaupun kedua teknik telah menggunakan sistem automatis dari tahap isolasi dan pembacaan analisa hasil. Dapat disimpulkan bahwa teknik AR memiliki performa yang lebih baik dalam mendeteksi virus HPV bila dibandingkan dengan teknik CB
Diagnosis Schistosomiasis dengan Metode Dot Blot
No full textThe diagnosis of schistosomiasis to detect secretory excretory antigen (AgES) Schistosoma japonicum by ELISA method, has been developed since 2012 through laboratory tests with good result, so it is necessary to do field test. Based on this the researchers conducted field tests, of schistosomiasis with dot blot in Napu, Poso district, Central Sulawesi Province. This study was conducted for nine months (March-November 2014) with cross sectional design. This study aims to determine the sensitivity and specificity of ELISA developed (dot blot). Fecal and blood surveys were conducted in communities in selected endemic villages, using simple random sampling method, with a total sample of 325 people. Furthermore, the blood serum tested using ELISA with dot blot technique. The sensitivity and specificity of the blot technique was calculated againts the results of microscopic examination (gold standard). Field test result showed the sensitivity and specificity of dot blot was 74% and 78%. Conclusion dot blot technique can detect Schistosomiasis on a laboratory scale or on a field scale.AbstrakDiagnosis schistosomiasis untuk mendeteksi antigen ekskretori sekretori (AgES) Schistosoma japonicum (S. japonicum) dengan metode ELISA, telah dikembangkan sejak tahun 2012 melalui uji laboratorium dengan hasil cukup baik, sehingga perlu dilakukan uji lapangan. Berdasarkan hal tersebut peneliti melakukan uji lapangan diagnosis schistosomiasis dengan dot blot di Napu, Kabupaten Poso, Provinsi Sulawesi Tengah. Penelitian dilaksanakan selama sembilan bulan (Maret - November 2014) dengan desain cross sectional. Penelitian ini bertujuan untuk menentukan sensitivitas dan spesifisitas dot blot yang dikembangkan. Survei tinja dan darah dilakukan pada masyarakat di desa endemis terpilih dengan metode simple random sampling, dengan jumlah sampel sebanyak 325 orang. Selanjutnya serumnya diuji ELISA dengan teknik dot blot. Sensitivitas dan spesifisitas teknik dot blot dihitung terhadap hasil pemeriksaan mikroskopis (gold standard). Hasil uji lapangan menunjukkan nilai sensitivitas dan spesifisitas dari teknik dot blot adalah sebesar 74% dan 78%. Kesimpulan teknik dot blot dapat mendeteksi schistosomiasis pada skala laboratorium maupun pada skala lapangan
Detection of Genotype D8 Measles Virus in Indonesia in 2014
No full textLatar Belakang: Campak adalah salah satu penyakit menular dan dapat menyebabkan penyakit serius sampai kematian. Campak masih merupakan masalah kesehatan masyarakat karena wabah campak masih terjadi di seluruh Indonesia. Surveilans berbasis laboratorium berikut penyelidikan epidemiologi molekuler memiliki kontribusi besar untuk mencegah wabah campak. Studi sebelumnya telah mendokumentasikan kehadiran genotipe virus campak G2, G3 dan D9 di Indonesia, dan genotipe lainnya seperti B3, D4, D5, D8 dan H1 telah terdeteksi di negara-negara tetangga. Penelitian ini bertujuan untuk mengkarakterisasi virus campak yang menyebabkan wabah di Indonesia pada tahun 2014. Metode: Tujuh puluh empat spesimen urin yang dikumpulkan dari delapan provinsi dan diperiksa oleh satu langkah RT-PCR dan metode sequencing Sanger. Analisis sekuensing dilakukan menggunakan Bioedit 7.1; DNAstar 7.0 dan software MEGA5.0. Hasil: Hasil PCR menunjukkan 34 dari 74 spesimen klinis positif dari virus campak. Kami menemukan genotipe dari 34 virus campak milik genotipe D8, D9 dan G3. Kesimpulan: Campak pertama genotipe D8 telah terdeteksi dari Indonesia pada tahun 2014 meskipun campak lainnya genotipe masih dapat ditemukan di Indonesia. Kata kunci: virus campak, D8 genotipe, wabah, Indonesia Background: Measles is a highly contagious viral disease. It remains an important cause of death among young children globally, despite the availability of a safe and effective vaccine. Measles is still a public health problem in Indonesia and measles outbreak still reported from many areas throughout Indonesia.. Molecular epidemiology of measles viruses is an important component in outbreak investigations to to monitor the presence of circulating wild–type measles strains.Previous studies in Indonesia have documented the presence of measles virus genotypes G2, G3 and D9 in Indonesia, and the other genotypes such as B3, D4, D5, D8 and H1 have been detected in neighboring countries. This study aims to characterize the measles virus that causing outbreak in Indonesia in 2014. Methods: Seventy four urine specimens were collected from eight provinces and examined by one step RT-PCR and Sanger sequencing method. Sequencing analysis were conducted using Bioedit 7.1; DNA Star 7.0 and MEGA 5.0 software. Results: The PCR results showed 34 out of 74 clinical specimens positive of measles virus. We found the genotype of 34 measles viruses belongs to genotype D8, D9 and G3. Conclusion: The first measles genotype D8 has been detected from Indonesia in 2014 although other measles genotype still can be found in Indonesia. Keywords: Measles virus, D8 genotype, outbreak, Indonesia
In vitro study of eight Indonesian plants extracts as anti Dengue virus
No full textLatar Belakang: Demam Berdarah Dengue (DBD) yang disebabkan oleh virus dengue masih merupakan masalah utama di negara tropis. Sampai saat ini belum tersedia vaksin maupun terapi yang efektif untuk DBD. Oleh karena itu, penelitian untuk menemukan antivirus spesifik untuk virus dengue sangat diperlukan. Indonesia kaya akan tumbuhan herbal yang mungkin berpotensi memiliki aktivitas antivirus, diantaranya adalah Psidium guajava (Jambu biji), Euphorbia hirta (Patikan kerbau), Piper bettle L. (Sirih), Carica papaya (Pepaya), Curcuma longa L. (Kunyit/turmeric), Phyllanthus niruri L. (Meniran), Andrographis paniculata (Sambiloto), dan Cymbopogon citratus (Serai). Penelitian sebelumnya menunjukkan bahwa beberapa tumbuhan herbal tersebut memiliki khasiat antibakteri, antivirus maupun keduanya. Namun, penelitian yang mengeksplorasi potensi beberapa herbal tersebut dalam melawan virus dengue masih terbatas. Tujuan: Penelitian ini bertujuan untuk mengetahui apakah beberapa tanaman herbal tersebut berpotensi memiliki aktivitas antivirus terhadap virus dengue secara in vitro. Metode: Ekstrak daun dari delapan tanaman herbal asli Indonesia diperoleh dari Solo, Jawa Tengah. Ekstrak kasar herbal tersebut diuji secara in vitro terhadap dengue virus serotype 2 (DENV-2) strain NGC menggunakan cell line Huh7it-1. Aktivitas antivirus beberapa ekstrak kasar tersebut diskrining dengan dosis 20mg/mL. Ekstrak yang menunjukkan aktivitas penghambatan virus dengue, selanjutnya diuji dengan variasi dosis untuk menentukan CC50 and IC50. Hasil: Dari uji penapisan awal terhadap 8 ekstrak tanaman herbal dengan dosis 20 mg/mL, Psidium guajava (Jambu biji) dan Carica papaya (Pepaya) memiliki efek sitotoksik sebesar 11,3% dan 2,5% dan mampu menghambat replikasi virus dengue masing-masing hingga 92,6% dan 89,5%. Dose dependent assay pada P. guajava menunjukkan CC50, IC50 dan indeks selektivitas berturut-turut sebesar 153,18 μg/mL, 7,2 μg/mL dan 21,28. Sedangkan C. papaya menunjukkan CC50, IC50 dan indeks selektivitas berturut-turut sebesar 244,76 μg/mL, 6,57 μg/mL dan 37,25. Kesimpulan: Psidium guajava dan Carica papaya memiliki potensi aktivitas antivirus melawan virus dengue in vitro. Kata kunci : Virus Dengue, ekstrak herbal, aktivitas antivirus, Psidium guajava, Carica papaya. Background: Dengue hemorrhagic fever (DHF) caused by dengue viruses is still a major problem in tropical countries. Until nowadays, there is no vaccine or effective therapy is available as yet. Thus research on discovering specific antiviral against dengue is needed. Indonesia is rich in indigenous herbal plants, which may has potential antiviral activity, such as Psidium guajava (Jambu biji), Euphorbia hirta (Patikan kerbau), Piper bettle L. (Sirih), Carica papaya (Pepaya), Curcuma longa L. (Kunyit/turmeric), Phyllanthus niruri L. (Meniran), Andrographis paniculata (Sambiloto), and Cymbopogon citratus (Serai). Previous studies showed that these plants, some have antibacterial properties, antiviral properties or both. However, there is only limited study of these plants against dengue virus. Objective: The aim of this study is to know whether these plants have potential activity against dengue virus in vitro. Method: Leave extracts of eight indigenous herbal plants as mentioned before were originated from Solo, Central Java. The crude extracts were tested in vitro against dengue virus serotype 2 (DENV-2) strain NGC using Huh7it-1 cell line. Those crude extracts were screened for antiviral activity using doses of 20 mg/mL. Candidates that showed inhibition activity were further tested in various doses to determine CC50 and IC50. Result: From eight leave extracts tested with 20 mg/mL dose, Psidium guajava (Jambu biji) and Carica papaya (Pepaya) have cytotoxicity 11.3% and 2.5% respectively and inhibited virus replication up to 92.6% and 89.5% respectively. Dose dependent assay of Psidium guajava showed CC50, IC50 and selectivity index 153.18 μg/mL, 7.2 μg/mL and 21.28 respectively. Whereas, C. papaya showed CC50, IC50 and selectivity index 244.76 μg/mL, 6.57 μg/mL and 37.25 respectively. Conclusion: Psidium guajava and Carica papaya have potential antiviral activity against dengue virus in vitro. Keywords: Dengue virus, natural extract, antiviral activity, Psidium guajava, Carica papaya